BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,972,637	2 bp→GC	coding (430‑431/546 nt)	wecD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,972,637	0	C	G	100.0%	1638.8 / NA	486	R144A (CGT→GCT)	wecD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (223/263); total (223/263)
*	NC_000913	3,972,638	0	G	C	100.0%	1646.5 / NA	486	R144A (CGT→GCT)	wecD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (223/263); total (223/263)

Only 100 of 486 total aligned reads displayed.

CTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972550‑3972724

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 tCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTcgc‑g                                                                                   >  2:3445226/1‑93 (MQ=255)

   aaTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGt                                                                                 <  2:6342592/93‑1 (MQ=255)

    aTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTa                                                                                >  2:1342635/1‑93 (MQ=255)

      gCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTaaa                                                                              >  2:3122434/1‑93 (MQ=255)

      gCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTaaa                                                                              >  2:4030665/1‑93 (MQ=255)

       cGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTaaaa                                                                             <  2:1234794/93‑1 (MQ=255)

       cGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTaaaa                                                                             <  1:5810124/93‑1 (MQ=255)

        gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAaac                                                                            >  1:5666182/1‑93 (MQ=255)

        gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAaac                                                                            <  1:4036832/93‑1 (MQ=255)

         aCAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGGTaaaaa                                                                            >  2:5954949/1‑92 (MQ=255)

          cAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAaacaa                                                                          >  1:6275261/1‑93 (MQ=255)

           aGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAaacaac                                                                         >  1:2786081/1‑93 (MQ=255)

           aGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAaacaac                                                                         >  2:6549487/1‑93 (MQ=255)

            gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACt                                                                        <  1:1622814/93‑1 (MQ=255)

            gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACt                                                                        >  2:4103664/1‑93 (MQ=255)

              tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACttt                                                                      >  2:6248688/1‑93 (MQ=255)

              tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACttt                                                                      <  2:5762715/93‑1 (MQ=255)

              tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACttt                                                                      <  2:7058615/93‑1 (MQ=255)

                cgcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGc                                                                    <  1:3706631/93‑1 (MQ=255)

                 gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCg                                                                   >  2:1906800/1‑93 (MQ=255)

                   gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCggg                                                                 >  2:3838867/1‑93 (MQ=255)

                    aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGt                                                                <  2:6060288/93‑1 (MQ=255)

                    aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGt                                                                >  1:413391/1‑93 (MQ=255)

                      ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTgg                                                              >  1:2358663/1‑93 (MQ=255)

                      ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTgg                                                              <  2:148075/93‑1 (MQ=255)

                       tGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGc                                                             <  1:4505783/93‑1 (MQ=255)

                           ctgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGAcc                                                         >  1:1836928/1‑93 (MQ=255)

                            tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGAccc                                                        >  2:2144563/1‑93 (MQ=255)

                            tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGAccc                                                        >  1:5015293/1‑93 (MQ=255)

                             gctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCa                                                       >  1:546034/1‑93 (MQ=255)

                               tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     <  1:2198568/93‑1 (MQ=255)

                               tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     <  2:5388407/93‑1 (MQ=255)

                               tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     <  2:7257085/93‑1 (MQ=255)

                               tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     >  1:1379055/1‑93 (MQ=255)

                                 gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   <  2:4775761/93‑1 (MQ=255)

                                 gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   >  2:5530048/1‑93 (MQ=255)

                                   tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATggg                                                 <  1:5971413/93‑1 (MQ=255)

                                    ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  1:1966489/93‑1 (MQ=255)

                                      aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa                                              <  2:2576113/93‑1 (MQ=255)

                                      aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa                                              <  1:6487847/93‑1 (MQ=255)

                                       cgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAac                                             <  1:6195827/93‑1 (MQ=255)

                                        gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca                                            >  1:807175/1‑93 (MQ=255)

                                        gcgGTGCAGGTGCTGAGCTGATGCAAAC‑GCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac                                           <  2:6729060/93‑1 (MQ=255)

                                         cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac                                           <  1:2949792/93‑1 (MQ=255)

                                         cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac                                           <  2:739222/93‑1 (MQ=255)

                                          ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc                                          >  1:7049313/1‑93 (MQ=255)

                                           gTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg                                         >  1:7225088/1‑93 (MQ=255)

                                           gTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg                                         >  2:3904467/1‑93 (MQ=255)

                                           gTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg                                         >  1:4708393/1‑93 (MQ=255)

                                            tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgc                                        >  2:6760856/1‑93 (MQ=255)

                                            tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgc                                        <  1:3465706/93‑1 (MQ=255)

                                            tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgc                                        <  1:3234604/93‑1 (MQ=255)

                                             gCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc                                       >  1:971343/1‑93 (MQ=255)

                                             gCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc                                       >  2:7516537/1‑93 (MQ=255)

                                                ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCcgcg                                    >  1:1041985/1‑93 (MQ=255)

                                                ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCcgcg                                    >  2:5693713/1‑93 (MQ=255)

                                                ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCcgcg                                    <  2:3344802/93‑1 (MQ=255)

                                                    ctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTa                                <  1:3114320/93‑1 (MQ=255)

                                                     tgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa                               >  1:5856738/1‑93 (MQ=255)

                                                     tgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa                               <  2:283826/93‑1 (MQ=255)

                                                      gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa                              >  1:5220995/1‑93 (MQ=255)

                                                      gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa                              >  1:729781/1‑93 (MQ=255)

                                                      gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa                              <  2:2756232/93‑1 (MQ=255)

                                                       agctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc                             <  2:5261094/93‑1 (MQ=255)

                                                       agctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc                             <  1:7459935/93‑1 (MQ=255)

                                                        gctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACg                            >  1:1283900/1‑93 (MQ=255)

                                                         ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           <  1:4327614/93‑1 (MQ=255)

                                                           gaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         <  2:2977249/93‑1 (MQ=255)

                                                           gaTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:5578271/1‑93 (MQ=255)

                                                            aTGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        >  2:2661331/1‑93 (MQ=255)

                                                             tGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTTGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       <  1:2060824/93‑1 (MQ=255)

                                                             tGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       >  1:4881093/1‑93 (MQ=255)

                                                             tGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       <  1:3081960/93‑1 (MQ=255)

                                                             tGCAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGacaca                       <  1:6595634/93‑1 (MQ=255)

                                                               cAAACGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacata                     >  2:3729051/1‑93 (MQ=255)

                                                                aaaCGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacatac                    <  1:6528040/93‑1 (MQ=255)

                                                                 aaCGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   <  1:1551326/93‑1 (MQ=255)

                                                                 aaCGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   >  2:6967991/1‑93 (MQ=255)

                                                                  aCGGCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaa                  >  1:6800846/1‑93 (MQ=255)

                                                                    ggCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAg                <  1:3397211/93‑1 (MQ=255)

                                                                    ggCGCTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAg                >  2:1684097/1‑93 (MQ=255)

                                                                     gcgcTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgt               <  2:5620385/93‑1 (MQ=255)

                                                                     gcgcTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgt               >  2:3232951/1‑93 (MQ=255)

                                                                     gcgcTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgt               >  2:2367546/1‑93 (MQ=255)

                                                                      cgcTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtg              >  1:2451725/1‑93 (MQ=255)

                                                                      cgcTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtg              <  1:6424076/93‑1 (MQ=255)

                                                                      cgcTAAACTGCGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCAAAATGGGCCACACCGCCGCGCTTAAACGATACATACAAAgtg              <  2:4857495/93‑1 (MQ=255)

                                                                       gcTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  1:1169016/1‑93 (MQ=255)

                                                                       gcTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  1:5154303/1‑93 (MQ=255)

                                                                        cTAAACTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            >  2:2034984/1‑93 (MQ=255)

                                                                           aaCTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCg         >  2:5800813/1‑93 (MQ=255)

                                                                            aCTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGa        >  1:2655254/1‑93 (MQ=255)

                                                                             cTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGaa       >  2:4299180/1‑93 (MQ=255)

                                                                             cTGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGaa       <  2:111983/93‑1 (MQ=255)

                                                                              tGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      >  1:5368671/1‑93 (MQ=255)

                                                                              tGGGCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      <  2:4534144/93‑1 (MQ=255)

                                                                               gggCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATg     >  2:686511/1‑93 (MQ=255)

                                                                               gggCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATg     >  2:4399761/1‑93 (MQ=255)

                                                                                ggCGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGt    <  2:5121147/93‑1 (MQ=255)

                                                                                  cGTATGCTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAg  <  1:3892666/93‑1 (MQ=255)

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CTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGC‑GGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972550‑3972724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`