BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,253,742	Δ1 bp	coding (551/552 nt)	yhaNM ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,253,741	0	G	.	99.6%	1035.2 / ‑3.8	231	coding (552/552 nt)	yhaNM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (122/108); minor base C (1/0); total (123/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA  >  NC_000913/3253649‑3253829

aTAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCg                                                                                          >  2:7830128/1‑93 (MQ=255)

aTAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCg                                                                                          >  2:3459379/1‑93 (MQ=255)

  aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgtt                                                                                        >  2:219621/1‑91 (MQ=255)

  aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgtt                                                                                        <  1:3097222/93‑3 (MQ=255)

  aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgtt                                                                                        >  1:2994086/1‑91 (MQ=255)

   aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       <  2:5275329/93‑4 (MQ=255)

   aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       <  1:6186509/93‑4 (MQ=255)

   aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       <  1:2100767/93‑4 (MQ=255)

   aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       <  1:256660/93‑4 (MQ=255)

   aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       <  1:2864245/93‑4 (MQ=255)

    cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttct                                                                                      <  2:6643266/93‑5 (MQ=255)

     gCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTg                                                                                    >  1:355839/1‑93 (MQ=255)

       acaATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACCTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGa                                                                                  >  1:232006/1‑93 (MQ=255)

        caATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGaa                                                                                 >  2:491076/1‑93 (MQ=255)

         aaTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGaaa                                                                                <  1:3801004/93‑1 (MQ=255)

          aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAg                                                                               >  2:5543340/1‑93 (MQ=255)

          aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAg                                                                               >  1:3221369/1‑93 (MQ=255)

          aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAg                                                                               <  2:1464846/93‑1 (MQ=255)

           tttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGc                                                                              <  1:1003999/93‑1 (MQ=255)

           tttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCAATGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTTGAAAGc                                                                              >  2:1033992/1‑93 (MQ=255)

            ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCa                                                                             <  1:4320423/93‑1 (MQ=255)

            ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCa                                                                             <  1:43464/93‑1 (MQ=255)

            ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCa                                                                             <  2:5748882/93‑1 (MQ=255)

            ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCa                                                                             >  1:6915278/1‑93 (MQ=255)

            ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCa                                                                             <  1:7561258/93‑1 (MQ=255)

             tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAc                                                                            <  1:5804300/93‑1 (MQ=255)

             tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAc                                                                            <  2:2819553/93‑1 (MQ=255)

             tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAc                                                                            <  2:2672120/93‑1 (MQ=255)

              aGCTTCGCGGAATCGAACATAAAGCGGATCGCCGCAAACGGGACTTCTTTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGGAAGCAcc                                                                           <  2:7042254/93‑1 (MQ=255)

                 ttCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTc                                                                        <  2:2980738/93‑1 (MQ=255)

                  tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCa                                                                       <  2:5397000/93‑1 (MQ=255)

                    gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGc                                                                     >  1:4021127/1‑93 (MQ=255)

                    gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGc                                                                     >  1:680710/1‑93 (MQ=255)

                     cgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCg                                                                    <  1:7639551/93‑1 (MQ=255)

                      ggAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCgg                                                                   >  1:6250832/1‑93 (MQ=255)

                        aaTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgag                                                                 >  2:5189968/1‑93 (MQ=255)

                         aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgaga                                                                <  1:3453463/93‑1 (MQ=255)

                          tCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAc                                                               <  2:7218345/93‑1 (MQ=255)

                           cGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAct                                                              <  1:2641847/93‑1 (MQ=255)

                            gagaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActc                                                             >  2:4187045/1‑93 (MQ=255)

                             agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActct                                                            <  1:480764/93‑1 (MQ=255)

                             agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActct                                                            <  1:1980498/93‑1 (MQ=255)

                              gaATAAAGCGGATCTCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCtt                                                           <  1:5493994/93‑1 (MQ=255)

                              gaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCtt                                                           <  2:6378505/93‑1 (MQ=255)

                              gaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCtt                                                           <  1:7887880/93‑1 (MQ=255)

                               aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTg                                                          <  1:4978282/93‑1 (MQ=255)

                               aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTg                                                          <  2:3907519/93‑1 (MQ=255)

                               aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTg                                                          >  1:943844/1‑93 (MQ=255)

                                aTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCATGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGc                                                         <  2:6350565/93‑1 (MQ=255)

                                     gCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTcccc                                                    >  1:1869035/1‑93 (MQ=255)

                                       ggATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGcccc                                                  <  2:2329160/93‑2 (MQ=255)

                                        gATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGccccc                                                 <  2:6068741/93‑3 (MQ=255)

                                         aTCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCccct                                                <  1:1422027/93‑1 (MQ=255)

                                           cgccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGc                                              <  2:4160222/93‑1 (MQ=255)

                                            gccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGcc                                             >  1:6414746/1‑93 (MQ=255)

                                               gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcaca                                          >  1:651083/1‑93 (MQ=255)

                                                cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacac                                         <  1:7803305/93‑1 (MQ=255)

                                                 aaaCGGGGCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                        <  2:3517841/93‑1 (MQ=255)

                                                 aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                        <  2:4030822/93‑1 (MQ=255)

                                                 aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                        <  1:2110213/93‑1 (MQ=255)

                                                  aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacac                                       >  1:519095/1‑93 (MQ=255)

                                                  aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacac                                       <  2:823514/93‑1 (MQ=255)

                                                     gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                    <  1:6728651/93‑1 (MQ=255)

                                                      ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCct                                   <  2:1977867/93‑1 (MQ=255)

                                                      ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCct                                   >  1:5541908/1‑93 (MQ=255)

                                                       gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                  >  1:1183692/1‑93 (MQ=255)

                                                       gACTTCATGGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                  <  2:3717362/93‑1 (MQ=255)

                                                        aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                 <  2:2407649/93‑1 (MQ=255)

                                                        aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                 <  2:6924883/93‑1 (MQ=255)

                                                        aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                 <  1:177818/93‑1 (MQ=255)

                                                        aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                 >  2:4986035/1‑93 (MQ=255)

                                                        aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                 >  2:7665973/1‑93 (MQ=255)

                                                          ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                               >  2:4568989/1‑93 (MQ=255)

                                                          ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                               >  1:5152475/1‑93 (MQ=255)

                                                          ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                               >  2:7440562/1‑93 (MQ=255)

                                                          ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                               >  2:3648377/1‑93 (MQ=255)

                                                           tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                              >  2:1718153/1‑93 (MQ=255)

                                                            cATTGACGATCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTggg                             <  1:4460813/93‑1 (MQ=255)

                                                            cATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTggg                             >  2:4232307/1‑93 (MQ=255)

                                                              ttGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTa                           >  1:7722643/1‑93 (MQ=255)

                                                              ttGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTa                           <  1:3238378/93‑1 (MQ=255)

                                                               tGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaa                          >  1:465151/1‑93 (MQ=255)

                                                                gACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaaa                         >  2:1323789/1‑93 (MQ=255)

                                                                 aCGAACTTCCGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTAAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCCGGGTAAac                        <  2:7324947/93‑1 (MQ=255)

                                                                  cGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAaca                       <  1:4245014/93‑1 (MQ=255)

                                                                  cGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAaca                       >  1:3300217/1‑93 (MQ=255)

                                                                  cGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAaca                       >  1:7366814/1‑93 (MQ=255)

                                                                   gAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacac                      >  2:2118332/1‑93 (MQ=255)

                                                                      cTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCCCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacaccac                   <  2:6857474/93‑1 (MQ=255)

                                                                           ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt              >  1:7122874/1‑93 (MQ=255)

                                                                           ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt              >  1:5294537/1‑93 (MQ=255)

                                                                           agATCTCAGCCAGCGTC‑GCTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt              <  1:6098927/92‑1 (MQ=255)

                                                                            gacctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATc             <  2:4454950/90‑1 (MQ=255)

                                                                              tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt           >  1:7830144/1‑93 (MQ=255)

                                                                                tcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGc         >  1:846038/1‑93 (MQ=255)

                                                                                tcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGc         <  1:1302418/93‑1 (MQ=255)

                                                                                  agccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGt       >  2:2621679/1‑93 (MQ=255)

                                                                                    ccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct     <  1:2931785/93‑1 (MQ=255)

                                                                                     cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc    <  1:7015212/93‑1 (MQ=255)

                                                                                       gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa  <  1:151450/93‑1 (MQ=255)

ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA  >  NC_000913/3253649‑3253829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`