BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,255,616	Δ1 bp	intergenic (‑19/+312)	yhaO ← / ← yhaP	putative transport system permease protein/putative L‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,255,615	0	G	.	100.0%	1633.7 / NA	358	intergenic (‑18/+313)	yhaO/yhaP	putative transport system permease protein/putative L‑serine dehydratase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (145/213); total (145/213)

Only 100 of 379 total aligned reads displayed.

TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCA  >  NC_000913/3255522‑3255703

tCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAg                                                                                           >  1:1579320/1‑93 (MQ=255)

gcaaaaTCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAg                                                                                           <  1:7121183/89‑1 (MQ=255)

 cATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgc                                                                                          >  2:2801758/1‑92 (MQ=255)

     aTCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCt                                                                                     >  2:1036556/1‑93 (MQ=255)

       cACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGc                                                                                   <  1:7011801/93‑1 (MQ=255)

        ccccAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCa                                                                                  <  1:6248988/92‑1 (MQ=255)

        aCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCa                                                                                  >  1:5513673/1‑93 (MQ=255)

         cccAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCaa                                                                                 >  1:3829497/1‑93 (MQ=255)

          ccAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAt                                                                                >  1:7926612/1‑93 (MQ=255)

            aGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGa                                                                              <  2:7649587/93‑1 (MQ=255)

            aGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGa                                                                              <  1:267952/93‑1 (MQ=255)

             gCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAc                                                                             <  1:4014792/93‑1 (MQ=255)

             gCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAc                                                                             >  2:1099022/1‑93 (MQ=255)

              ccGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCC‑GGCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACg                                                                            >  1:416817/1‑93 (MQ=255)

               cGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGc                                                                           <  2:3364307/93‑1 (MQ=255)

                ggTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGcc                                                                          >  1:7832275/1‑93 (MQ=255)

                  tgtCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCtt                                                                        <  2:6127661/93‑1 (MQ=255)

                  tgtCAGTACTGTCGAATTTGATCGCTTCTCACCGCTCCCGCTCCCTC‑CGACATCCACGCCCCGCCGGTGTCGAAGG‑GTCTGCCATTACGGCtt                                                                        <  2:661874/93‑1 (MQ=255)

                    tCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa                                                                      >  1:3774452/1‑93 (MQ=255)

                    tCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa                                                                      <  1:4236754/93‑1 (MQ=255)

                    tCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa                                                                      <  2:3979569/93‑1 (MQ=255)

                     cAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAt                                                                     <  2:5674013/93‑1 (MQ=255)

                     cAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAt                                                                     <  2:7489697/93‑1 (MQ=255)

                      aGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAtt                                                                    <  2:6808623/93‑1 (MQ=255)

                      aGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAtt                                                                    <  2:7771836/93‑1 (MQ=255)

                      aGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAtt                                                                    <  1:4480566/93‑1 (MQ=255)

                       gTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTc                                                                   <  2:2237410/93‑1 (MQ=255)

                       gTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTc                                                                   <  2:6971160/93‑1 (MQ=255)

                        tACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCg                                                                  >  1:4791429/1‑93 (MQ=255)

                         aCTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGa                                                                 >  1:5358368/1‑93 (MQ=255)

                         aCTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGa                                                                 <  1:7744183/93‑1 (MQ=255)

                          cTGTCGAATTTGATCGCTTCTCGCCCCTCGCTCTCACTCATTCCA‑GCACGCCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAt                                                                <  1:1206910/93‑1 (MQ=255)

                           tGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATg                                                               >  1:5126762/1‑93 (MQ=255)

                            gTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGc                                                              <  1:492685/93‑1 (MQ=255)

                            gTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGc                                                              >  1:1403967/1‑93 (MQ=255)

                             tCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCa                                                             >  2:1383131/1‑93 (MQ=255)

                              cGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCaa                                                            <  1:5681144/93‑1 (MQ=255)

                              cGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCaa                                                            <  2:54939/93‑1 (MQ=255)

                               gAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATCCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑t                                                          <  1:187933/93‑1 (MQ=255)

                                 aTTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑ttt                                                        <  1:1655540/93‑1 (MQ=255)

                                 aTTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑ttt                                                        >  1:6702423/1‑93 (MQ=255)

                                   ttGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTcc                                                      >  2:5183869/1‑93 (MQ=255)

                                    tGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCa                                                     <  1:521256/93‑1 (MQ=255)

                                       tCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTTCCATa                                                   <  2:6429464/93‑1 (MQ=255)

                                       tCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATaa                                                  <  2:2526684/93‑1 (MQ=255)

                                         gCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAAtt                                                <  1:1032023/93‑1 (MQ=255)

                                         gCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAAtt                                                >  1:7439432/1‑93 (MQ=255)

                                          cTTCTCGCCCCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAAttt                                               <  1:6492294/93‑1 (MQ=255)

                                          cTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAAttt                                               <  1:4601522/93‑1 (MQ=255)

                                          cTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTGATTCGATGCAA‑TTGCCATAAttt                                               <  2:4093538/93‑1 (MQ=255)

                                          cTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCCTTATTCGATGCTA‑TTTCCATAAttt                                               >  1:2850423/1‑93 (MQ=255)

                                           ttCTCGCGCCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTtc                                              <  2:2300933/93‑1 (MQ=255)

                                           ttCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTtc                                              <  1:3246905/93‑1 (MQ=255)

                                           ttCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTtc                                              <  2:2953425/93‑1 (MQ=255)

                                            tctcGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTtct                                             <  1:6173116/93‑1 (MQ=255)

                                                gCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATGCGATGCAA‑TTTCCATAATTTCTCGCt                                         >  2:3427310/1‑93 (MQ=255)

                                                 ccACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTc                                        >  2:1796168/1‑93 (MQ=255)

                                                 ccACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTc                                        <  1:6109251/93‑1 (MQ=255)

                                                  cACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCa                                       >  2:6709110/1‑93 (MQ=255)

                                                  cACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCa                                       >  2:2733305/1‑93 (MQ=255)

                                                   ccTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCaa                                      <  2:1295321/92‑1 (MQ=255)

                                                    cTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAat                                     >  2:3846778/1‑93 (MQ=255)

                                                    cTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAat                                     <  1:6793205/93‑1 (MQ=255)

                                                    cTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTGTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAat                                     <  1:1141304/93‑1 (MQ=255)

                                                     tCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTTCATAATTTCTCGCTCAata                                    <  1:7661180/93‑1 (MQ=255)

                                                      cGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAatat                                   >  1:2022428/1‑93 (MQ=255)

                                                      cGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAatat                                   <  2:7066078/93‑1 (MQ=255)

                                                        ctctCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATAttt                                 >  2:4533404/1‑93 (MQ=255)

                                                         tctcACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATAtttt                                >  2:4199265/1‑93 (MQ=255)

                                                          ctcactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTg                               <  2:7280233/93‑1 (MQ=255)

                                                           tcactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GAGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGt                              >  2:4956203/1‑93 (MQ=255)

                                                            cactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTa                             <  2:162088/93‑1 (MQ=255)

                                                              ctcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAgg                           <  2:2421326/93‑1 (MQ=255)

                                                              ctcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAgg                           >  2:6205774/1‑93 (MQ=255)

                                                               tcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAggg                          <  1:6364827/93‑1 (MQ=255)

                                                               tcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAggg                          >  2:5392692/1‑93 (MQ=255)

                                                                caTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGc                         <  1:2596696/93‑1 (MQ=255)

                                                                 aTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCt                        >  2:6090097/1‑93 (MQ=255)

                                                                 aTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCt                        >  2:4876686/1‑93 (MQ=255)

                                                                  ttCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCCATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATCTTTTGTAGGGCtt                       >  2:3124059/1‑93 (MQ=255)

                                                                    tccagCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCtta                      <  2:4397927/89‑1 (MQ=255)

                                                                       cgCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCttatta                   <  1:2495560/92‑1 (MQ=255)

                                                                       agCACGACCCGCCGGGGTCGAA‑GCNTCTGNAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCttatta                   <  2:4618997/92‑1 (MQ=255)

                                                                       agCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCttatta                   <  2:5974288/92‑1 (MQ=255)

                                                                        gCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTtattat                  <  1:7519604/93‑1 (MQ=255)

                                                                         cACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTattatt                 <  2:2684914/93‑1 (MQ=255)

                                                                          ccGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAttt                <  2:1963659/92‑1 (MQ=255)

                                                                          aCGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAttt                <  2:5321756/93‑1 (MQ=255)

                                                                          aCGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAttt                <  2:3678382/93‑1 (MQ=255)

                                                                           cGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAtttt               <  1:893648/93‑1 (MQ=255)

                                                                           cGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAAGCCTTTATTCGATGCAA‑TTTCGATAATTTCTCGCTCTATGTTTTGTAGGGCTTATTAtttt               >  2:5846032/1‑93 (MQ=255)

                                                                            gACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAttttt              <  1:2343570/93‑1 (MQ=255)

                                                                             aCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAtttttt             >  1:4865729/1‑93 (MQ=255)

                                                                             aCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAtttttt             <  1:228243/93‑1 (MQ=255)

                                                                                  ccgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGa        <  1:1946943/93‑1 (MQ=255)

                                                                                    ggggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGc      >  2:910205/1‑93 (MQ=255)

                                                                                     gggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGcc     <  1:3316206/93‑1 (MQ=255)

                                                                                     gggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGcc     <  1:5062600/93‑1 (MQ=255)

                                                                                      ggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCg    >  2:6749204/1‑93 (MQ=255)

                                                                                        tCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCa  >  1:6955298/1‑93 (MQ=255)

TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAA‑TTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCA  >  NC_000913/3255522‑3255703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`