Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,061,961 Δ1 bp coding (1386/1458 nt) b3876 ← putative permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,061,9590C.99.5% 1625.8 / ‑1.0 361coding (1388/1458 nt)b3876putative permease
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base . (207/152);  total (209/152)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 386 total aligned reads displayed.

TTTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTA  >  NC_000913/4061867‑4062047
                                                                                            |                                                                                        
tttttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGc                                                                                          >  1:93462/1‑93 (MQ=255)
tttttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGc                                                                                          >  2:153963/1‑93 (MQ=255)
tttttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGc                                                                                          <  2:4255504/93‑1 (MQ=255)
 ttttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGcc                                                                                         >  2:7282105/1‑93 (MQ=255)
  tttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGccg                                                                                        >  2:7672075/1‑92 (MQ=255)
     cATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGccgttt                                                                                     >  1:2459635/1‑89 (MQ=255)
      ataataAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGtttt                                                                                   >  1:6642425/1‑93 (MQ=255)
      ataataAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGtttt                                                                                   >  2:3763694/1‑93 (MQ=255)
       taataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGttttt                                                                                  >  1:6209882/1‑93 (MQ=255)
       taataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGttttt                                                                                  <  1:2278168/93‑1 (MQ=255)
        aataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGtttttt                                                                                 >  1:7134570/1‑93 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCGTtttttt                                                                                 >  2:2876348/1‑93 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttc                                                                                <  2:4701480/93‑1 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttc                                                                                <  2:827807/93‑1 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttc                                                                                <  1:6392545/93‑1 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTAAAACGTTACGGAAG‑CCGTTTTttc                                                                                >  2:5647744/1‑93 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGACGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCGTtttttt                                                                                 >  2:3315293/1‑93 (MQ=255)
          taaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTCACATTTATAACGTTACGGAAG‑CCGTTTTttct                                                                               >  1:2707564/1‑93 (MQ=255)
           aaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttctt                                                                              >  1:1098272/1‑93 (MQ=255)
            aGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttcttc                                                                             <  1:6686118/93‑1 (MQ=255)
            aGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttcttc                                                                             <  2:514147/93‑1 (MQ=255)
             gTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTtcttct                                                                            <  2:4006966/93‑1 (MQ=255)
              tCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTg                                                                           >  2:490018/1‑93 (MQ=255)
                cATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTc                                                                         <  2:4717724/93‑1 (MQ=255)
                 aTTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCg                                                                        >  2:5456618/1‑93 (MQ=255)
                 aTTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCg                                                                        >  2:3107417/1‑93 (MQ=255)
                  ttttttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGc                                                                       <  1:7896685/93‑1 (MQ=255)
                    ttttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCa                                                                     >  2:6478898/1‑93 (MQ=255)
                    ttttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCa                                                                     >  2:2134078/1‑93 (MQ=255)
                    ttttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCa                                                                     >  2:4568610/1‑93 (MQ=255)
                     tttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAt                                                                    >  2:4200984/1‑93 (MQ=255)
                      ttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtc                                                                   <  1:611111/93‑1 (MQ=255)
                        aaaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtctc                                                                 <  1:1039898/93‑1 (MQ=255)
                        aaaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtctc                                                                 <  2:3828254/93‑1 (MQ=255)
                         aaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATctct                                                                >  2:387178/1‑93 (MQ=255)
                         aaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATctct                                                                <  1:6893391/93‑1 (MQ=255)
                            gTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTa                                                             >  1:4764083/1‑93 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            >  1:5022263/1‑93 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            >  1:4337127/1‑93 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            >  2:1387157/1‑93 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            <  2:7475691/93‑1 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            <  1:7668885/93‑1 (MQ=255)
                              aGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAtt                                                           >  2:3516477/1‑93 (MQ=255)
                              aGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAtt                                                           <  1:3949582/93‑1 (MQ=255)
                                ggAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAtttt                                                         <  1:7407569/93‑1 (MQ=255)
                                      cgccACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgtt                                                   <  1:2999517/93‑1 (MQ=255)
                                      cgccACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgtt                                                   <  2:2712421/93‑1 (MQ=255)
                                       gccACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttg                                                  >  1:5329197/1‑93 (MQ=255)
                                        ccacccacCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttgg                                                 <  1:4651021/93‑1 (MQ=255)
                                        ccacccacCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttgg                                                 >  1:2644776/1‑93 (MQ=255)
                                         cacccaccAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CAGTTTTTTCTTCTGTCGCCATCTCTTTCTTTTCGCgttggt                                                >  1:284357/1‑93 (MQ=255)
                                          ccccaccAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttggtt                                               <  2:364492/92‑1 (MQ=255)
                                          acccaccAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttggtt                                               >  1:1795379/1‑93 (MQ=255)
                                              accaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAtt                                           <  1:3869661/93‑1 (MQ=255)
                                              accaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAtt                                           >  2:5554345/1‑93 (MQ=255)
                                               ccaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAttt                                          >  1:3297099/1‑93 (MQ=255)
                                               ccaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAttt                                          >  2:4156685/1‑93 (MQ=255)
                                               ccaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAttt                                          >  1:548077/1‑93 (MQ=255)
                                                 aGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTcc                                        <  2:2459919/93‑1 (MQ=255)
                                                  ggATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCt                                       >  1:3730255/1‑93 (MQ=255)
                                                   gATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTc                                      >  2:319994/1‑93 (MQ=255)
                                                    aTGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCa                                     >  1:4240229/1‑93 (MQ=255)
                                                       gAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaata                                  >  2:7112191/1‑93 (MQ=255)
                                                       gAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaata                                  <  1:5265809/93‑1 (MQ=255)
                                                         aaTAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaataat                                >  1:2437770/1‑93 (MQ=255)
                                                          aTAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATc                               >  1:2782606/1‑93 (MQ=255)
                                                           tAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCa                              >  2:2562246/1‑93 (MQ=255)
                                                           tAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTACTCAATAATCa                              >  1:540185/1‑93 (MQ=255)
                                                             gCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAAt                            <  2:2615044/93‑1 (MQ=255)
                                                               ggCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGc                          <  1:3579671/93‑1 (MQ=255)
                                                               ggCTATTGACATTTATAACGTTACGGAAG‑CCGGTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGc                          >  2:347022/1‑93 (MQ=255)
                                                                gCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCa                         >  1:4224765/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       >  1:3418316/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       >  2:5915245/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       >  1:1436377/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       >  2:7828911/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACCTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       <  2:1653840/93‑1 (MQ=255)
                                                                   aTTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAc                      >  1:6441851/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAcc                     >  2:2030227/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAcc                     >  2:2246345/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAcc                     >  1:164940/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAcc                     >  2:6909783/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCCCAATAATCAATGCaaccg                     >  1:4553855/1‑90 (MQ=255)
                                                                     tGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCCTCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCg                    >  1:4906081/1‑93 (MQ=255)
                                                                      gACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGt                   >  2:1295293/1‑93 (MQ=255)
                                                                         aTTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGtttt                <  1:3479697/93‑1 (MQ=255)
                                                                          tttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGttttt               >  2:1829741/1‑93 (MQ=255)
                                                                          tttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGttttt               >  2:6403015/1‑93 (MQ=255)
                                                                           ttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTc              <  2:5810368/93‑1 (MQ=255)
                                                                           ttATAACGTTACGGAAG‑CCGCTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCCATAATCAATGCAAACCGTTTTTc              >  2:6081525/1‑93 (MQ=255)
                                                                            tataACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCa             <  1:823962/93‑1 (MQ=255)
                                                                               aaCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTg          <  1:5699828/93‑1 (MQ=255)
                                                                               aaCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTg          >  2:4964583/1‑93 (MQ=255)
                                                                               aaCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTg          >  1:1979316/1‑93 (MQ=255)
                                                                                aCGTTCCGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGa         <  2:6703797/93‑1 (MQ=255)
                                                                                aCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGa         >  2:618189/1‑93 (MQ=255)
                                                                                 cGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAg        <  1:5917729/93‑1 (MQ=255)
                                                                                   ttACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtg      >  1:5999114/1‑93 (MQ=255)
                                                                                    tACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtgt     <  1:495977/93‑1 (MQ=255)
                                                                                     aCGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtgtg    <  2:1104765/93‑1 (MQ=255)
                                                                                            |                                                                                        
TTTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTA  >  NC_000913/4061867‑4062047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑