Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,644,603 (G)6→5 coding (743/2313 nt) pbpC ← putative peptidoglycan enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,644,5980G.100.0% 1053.5 / NA 232coding (748/2313 nt)pbpCputative peptidoglycan enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (126/106);  total (126/106)

Only 100 of 265 total aligned reads displayed.

AAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTA  >  NC_000913/2644506‑2644686
                                                                                            |                                                                                        
 agcccGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgg                                                                                         <  2:5502463/90‑1 (MQ=255)
  gACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCggg                                                                                        >  1:6914139/1‑93 (MQ=255)
    ccGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCggggg                                                                                      >  1:6404469/1‑93 (MQ=255)
     gggCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCCTTATGCGCGAAAACAGCGGGGCCAGGTGCGGGATTTGTggggggc                                                                                     >  2:2783049/2‑92 (MQ=255)
     cGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggc                                                                                     <  1:1638627/93‑2 (MQ=255)
      ggCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggcc                                                                                    <  1:6052059/93‑3 (MQ=255)
       gCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggcca                                                                                   <  2:4320446/93‑4 (MQ=255)
       gCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggcca                                                                                   >  1:5382881/1‑90 (MQ=255)
       gCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggcca                                                                                   <  1:7799186/93‑4 (MQ=255)
        cATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggccag                                                                                  >  2:1807844/1‑89 (MQ=255)
          tCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagcc                                                                               >  2:3397516/1‑93 (MQ=255)
          tCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagcc                                                                               <  1:1467627/93‑1 (MQ=255)
           ccAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagcca                                                                              <  2:2664636/93‑1 (MQ=255)
             aaCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGa                                                                            <  1:524179/93‑1 (MQ=255)
              aCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAt                                                                           <  2:100396/93‑1 (MQ=255)
              aCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAt                                                                           >  1:4498112/1‑93 (MQ=255)
               cGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATg                                                                          <  1:7422380/93‑1 (MQ=255)
               cGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATg                                                                          >  1:578072/1‑93 (MQ=255)
                gtagtaGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGccggccagtggg                                                                         >  2:3998501/1‑89 (MQ=255)
                gtagtaGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATgg                                                                         <  1:3146606/93‑1 (MQ=255)
                gtagtaGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATgg                                                                         <  1:6870888/93‑1 (MQ=255)
                gtagtaGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATgg                                                                         >  1:6783660/1‑93 (MQ=255)
                gtagtaGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATgg                                                                         >  2:4147443/1‑93 (MQ=255)
                 tagtagTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATggg                                                                        >  1:1017504/1‑93 (MQ=255)
                   gtagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGtt                                                                      <  1:46239/93‑1 (MQ=255)
                   gtagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGtt                                                                      <  2:6121663/93‑1 (MQ=255)
                   gtagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGAGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCAAACTGGGtt                                                                      >  2:5872819/1‑93 (MQ=255)
                    tagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGttc                                                                     >  1:4055505/1‑93 (MQ=255)
                         aTTTTGGCGCTTTTGCTTTTCCCGAGCATCATGCGCGAAAACCGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTccc                                                                >  2:7100509/1‑93 (MQ=255)
                          ttttGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCt                                                               >  1:5598709/1‑93 (MQ=255)
                           tttGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCtt                                                              <  1:3238661/93‑1 (MQ=255)
                            ttGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCTGATGGGTTCTTCCCTTg                                                             >  1:7048176/1‑93 (MQ=255)
                                cGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActt                                                         >  2:2328693/1‑92 (MQ=255)
                                cGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActc                                                         <  2:3154713/93‑1 (MQ=255)
                                cGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActc                                                         <  1:7672433/93‑1 (MQ=255)
                                cGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActc                                                         <  1:3773251/93‑1 (MQ=255)
                                 gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActct                                                        >  1:6138632/1‑93 (MQ=255)
                                 gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActct                                                        >  2:6763093/1‑93 (MQ=255)
                                  cttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtt                                                       >  2:7446597/1‑93 (MQ=255)
                                  cttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtt                                                       >  1:2429466/1‑93 (MQ=255)
                                   ttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCttt                                                      <  1:2259025/93‑1 (MQ=255)
                                   ttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCttt                                                      <  2:5270621/93‑1 (MQ=255)
                                   ttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCttt                                                      >  2:6174379/1‑93 (MQ=255)
                                   ttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGCGCCAGCCAGATGCGCGCTTCCCTTGACTCttt                                                      >  2:2495836/1‑93 (MQ=255)
                                    tttgcttttACCGAGCATCATTCGCGAAAACAGCGATGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtttt                                                     >  1:1351122/1‑93 (MQ=255)
                                    tttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtttt                                                     >  2:6045775/1‑93 (MQ=255)
                                    tttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtttt                                                     >  2:4930776/1‑93 (MQ=255)
                                    tttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtttt                                                     <  2:1759566/93‑1 (MQ=255)
                                     ttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTa                                                    <  1:4428504/93‑1 (MQ=255)
                                     ttgcttttACCGAGCATCATGCGCGAAAACAGCGGTG‑CAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTAc                                                   <  2:2709299/93‑1 (MQ=255)
                                      tgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTAc                                                   >  1:1849240/1‑93 (MQ=255)
                                       gcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTAcc                                                  <  2:4618083/93‑1 (MQ=255)
                                       gcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTAcc                                                  <  2:1363444/93‑1 (MQ=255)
                                        cttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCt                                                 <  2:4044908/93‑1 (MQ=255)
                                        cttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCt                                                 >  2:2940702/1‑93 (MQ=255)
                                        cttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCt                                                 >  2:5559162/1‑93 (MQ=255)
                                        cttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGATCTTCCCTTGACTCTTTTACCt                                                 <  2:4981546/93‑1 (MQ=255)
                                          tttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGc                                               <  2:1876596/93‑1 (MQ=255)
                                          tttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGc                                               >  1:6987282/1‑93 (MQ=255)
                                            tACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTc                                             <  2:6397025/93‑1 (MQ=255)
                                             aCCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCa                                            <  1:2443408/93‑1 (MQ=255)
                                                gAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACgg                                         >  1:195751/1‑93 (MQ=255)
                                                gAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACgg                                         >  2:4594819/1‑93 (MQ=255)
                                                 aGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACTTGCTCACggg                                        <  1:4464513/93‑1 (MQ=255)
                                                 aGCATCATGCGCGAAAACAGCGGTGACAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACggg                                        <  1:5797694/93‑1 (MQ=255)
                                                  gCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGa                                       >  2:819724/1‑93 (MQ=255)
                                                  gCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGa                                       <  1:6256496/93‑1 (MQ=255)
                                                     tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACca                                    >  1:2498548/1‑93 (MQ=255)
                                                     tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACca                                    >  2:6191143/1‑93 (MQ=255)
                                                       atGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcaca                                  <  1:4793491/93‑1 (MQ=255)
                                                        tGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcacac                                 >  2:6343164/1‑93 (MQ=255)
                                                        tGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGCGACcacac                                 >  2:88892/1‑93 (MQ=255)
                                                           gcgAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACAcc                              <  1:7568892/93‑1 (MQ=255)
                                                             gAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCtt                            <  1:5938418/93‑1 (MQ=255)
                                                              aaaaCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTg                           >  2:4347303/1‑93 (MQ=255)
                                                                aaCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCa                         <  2:4305350/93‑1 (MQ=255)
                                                                aaCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCa                         >  2:3316601/1‑93 (MQ=255)
                                                                aaCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCa                         <  2:6931373/93‑1 (MQ=255)
                                                                  cAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACg                       >  2:207354/1‑93 (MQ=255)
                                                                  cAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACg                       >  1:3594587/1‑93 (MQ=255)
                                                                   aGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGGTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACgg                      >  1:2613018/1‑93 (MQ=255)
                                                                    gCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGc                     >  1:7465326/1‑93 (MQ=255)
                                                                    gCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGc                     <  1:4884982/93‑1 (MQ=255)
                                                                       gTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCAt                  >  1:1243743/1‑93 (MQ=255)
                                                                        tGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATc                 <  2:7130682/93‑1 (MQ=255)
                                                                        tGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATc                 <  1:2656243/93‑1 (MQ=255)
                                                                         gCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATcc                >  2:7603153/1‑93 (MQ=255)
                                                                         gCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATcc                >  2:3702417/1‑93 (MQ=255)
                                                                          ccAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCg               >  1:7224017/1‑93 (MQ=255)
                                                                           cAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGt              <  1:5573371/93‑1 (MQ=255)
                                                                           cAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGt              <  1:3415468/93‑1 (MQ=255)
                                                                             gTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTc            <  1:2867068/93‑1 (MQ=255)
                                                                              ttGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCg           >  2:4723499/1‑93 (MQ=255)
                                                                               tGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGa          <  1:2769712/93‑1 (MQ=255)
                                                                                 cGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGc        <  2:1533222/93‑1 (MQ=255)
                                                                                  ggCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCa       <  1:6480432/93‑1 (MQ=255)
                                                                                  ggCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCa       <  2:7717133/93‑1 (MQ=255)
                                                                                     aTTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACtt    >  1:6331530/1‑93 (MQ=255)
                                                                                       ttGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTtta  <  2:5826093/93‑1 (MQ=255)
                                                                                       ttGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTtta  <  2:7908322/93‑1 (MQ=255)
                                                                                            |                                                                                        
AAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTA  >  NC_000913/2644506‑2644686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑