BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,339,304	+C	coding (398/987 nt)	yrbH →	putative isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,339,301	1	.	C	98.9%	1097.9 / 2.5	354	coding (395/987 nt)	yrbH	putative isomerase
Reads supporting (aligned to +/- strand): ref base . (1/3); new base C (176/173); total (178/176)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 399 total aligned reads displayed.

GCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑CCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGAC  >  NC_000913/3339210‑3339388

 cTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑cc                                                                                       <  2:4194819/93‑1 (MQ=255)

  tATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑ccc                                                                                      <  1:818092/93‑1 (MQ=255)

  tATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑ccc                                                                                      <  1:4074289/93‑1 (MQ=255)

   aTCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑cccc                                                                                     >  2:7438085/1‑92 (MQ=255)

    tctcTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑ccccg                                                                                    >  1:7088413/1‑91 (MQ=255)

    tctcTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑ccccg                                                                                    >  1:6593660/1‑91 (MQ=255)

    tctcTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑ccccg                                                                                    >  2:5048985/1‑91 (MQ=255)

          aCTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGagc                                                                               <  2:7818887/93‑1 (MQ=255)

          aCTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGagc                                                                               <  1:9912/93‑1 (MQ=255)

            ctgggTGAATCCAGCGAAATCGCGGCCTTAATTCCAGTGCGTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGagcag                                                                             <  2:4453727/90‑1 (MQ=255)

             ctGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGagcagc                                                                            >  2:3763067/1‑93 (MQ=255)

             ctGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGagcagc                                                                            >  1:4151893/1‑93 (MQ=255)

              tGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAgcagca                                                                           <  2:765628/93‑1 (MQ=255)

              tGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAgcagca                                                                           <  2:2500065/93‑1 (MQ=255)

              tGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAgcagca                                                                           <  1:3098198/93‑1 (MQ=255)

              tGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAgcagca                                                                           <  1:3590148/93‑1 (MQ=255)

              tGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAgcagca                                                                           <  2:5253872/93‑1 (MQ=255)

               ggTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGCGCAGCAt                                                                          >  2:1524827/1‑93 (MQ=255)

               ggTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCAt                                                                          <  1:1679316/93‑1 (MQ=255)

                 tGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATgg                                                                        >  1:4905308/1‑93 (MQ=255)

                    aTCCAGCGATATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGgcgc                                                                     <  2:5330316/93‑1 (MQ=255)

                     tCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGgcgcg                                                                    >  2:7209513/1‑93 (MQ=255)

                         gttaaaTCACGGCCTTTATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGTGCAGCATGGCTCGcgcc                                                                >  2:7032848/4‑93 (MQ=255)

                         gCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGcgcc                                                                <  1:2268007/93‑1 (MQ=255)

                          cGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGcgccg                                                               >  1:7175851/1‑93 (MQ=255)

                           gAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGcgccgc                                                              <  1:7027644/93‑1 (MQ=255)

                           gAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGcgccgc                                                              >  1:5111462/1‑93 (MQ=255)

                            aaaTCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCa                                                             >  1:3711519/1‑93 (MQ=255)

                            aaaTCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCa                                                             >  2:125032/1‑93 (MQ=255)

                              aTCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGa                                                           <  1:7876373/93‑1 (MQ=255)

                              aTCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGa                                                           <  2:3109835/93‑1 (MQ=255)

                               tCACGGCCTTGATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGAt                                                          >  2:5091676/1‑93 (MQ=255)

                                 aCGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGAtgt                                                        >  1:1128202/1‑93 (MQ=255)

                                 aCGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGAtgt                                                        >  1:6804316/1‑93 (MQ=255)

                                  cGGCCTTAATTCCAGTGCTTAATCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGAtgtg                                                       >  1:1409746/1‑93 (MQ=255)

                                  cGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGAtgtg                                                       <  1:2544223/93‑1 (MQ=255)

                                   ggCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGc                                                      >  1:5314430/1‑93 (MQ=255)

                                   ggCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGc                                                      <  2:4560603/93‑1 (MQ=255)

                                      cTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATc                                                   <  2:2111634/93‑1 (MQ=255)

                                      cTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATc                                                   >  2:6264634/1‑93 (MQ=255)

                                       ttAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCt                                                  <  2:6513476/93‑1 (MQ=255)

                                       ttAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCt                                                  >  2:7075575/1‑93 (MQ=255)

                                         aaTTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgt                                                <  1:6242130/93‑1 (MQ=255)

                                          aTTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgtg                                               <  1:6427039/93‑1 (MQ=255)

                                           ttCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgtgt                                              <  1:260103/93‑1 (MQ=255)

                                              cAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgtgtggt                                           >  2:788448/1‑91 (MQ=255)

                                                   cTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGt                                      >  2:5842604/1‑93 (MQ=255)

                                                   cTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGt                                      <  2:4926695/93‑1 (MQ=255)

                                                   cTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGt                                      <  2:1373257/93‑1 (MQ=255)

                                                    ttAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTa                                     <  1:2465746/93‑1 (MQ=255)

                                                    ttAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTa                                     >  1:5220386/1‑93 (MQ=255)

                                                    ttAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTa                                     <  2:664218/93‑1 (MQ=255)

                                                    ctAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTa                                     <  2:5957283/92‑1 (MQ=255)

                                                     tAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAg                                    >  1:4038728/1‑93 (MQ=255)

                                                     tAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAg                                    >  2:2230947/1‑93 (MQ=255)

                                                      aaGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGc                                   >  2:6204152/1‑93 (MQ=255)

                                                       aGCGTCTTCACGTACCGTTAATTTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCg                                  >  1:5592993/1‑93 (MQ=255)

                                                         cGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGaa                                <  1:1582708/93‑1 (MQ=255)

                                                          gTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGaaa                               <  1:4285304/93‑1 (MQ=255)

                                                          gTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGaaa                               >  1:6387674/1‑93 (MQ=255)

                                                          gTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGaaa                               <  1:4516754/93‑1 (MQ=255)

                                                           tCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaag                              >  2:478260/1‑93 (MQ=255)

                                                           tCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaag                              >  1:4614999/1‑93 (MQ=255)

                                                           tCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaag                              <  1:1004012/93‑1 (MQ=255)

                                                           gctgcACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaag                              <  2:6882308/89‑1 (MQ=255)

                                                            cTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTTAAGTAGCGAaaga                             >  2:2892552/1‑93 (MQ=255)

                                                             ttCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaagaa                            <  2:5766930/93‑1 (MQ=255)

                                                             ttCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaagaa                            <  1:2730557/93‑1 (MQ=255)

                                                             ttCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaagaa                            <  2:5155407/93‑1 (MQ=255)

                                                               cACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGc                          <  2:5621218/93‑1 (MQ=255)

                                                                aCGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGcc                         >  1:5034170/1‑93 (MQ=255)

                                                                aCGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGcc                         <  1:5413516/93‑1 (MQ=255)

                                                                aCGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGcc                         <  2:1797199/93‑1 (MQ=255)

                                                                aCGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGcc                         >  1:3307343/1‑93 (MQ=255)

                                                                 cGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCt                        <  2:4049485/93‑1 (MQ=255)

                                                                  gTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTg                       >  2:6092287/1‑93 (MQ=255)

                                                                    aCCGTTAATCTGCATCACCGGTCAC‑CCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTc                     <  2:5405816/93‑1 (MQ=255)

                                                                     ccGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTcc                    >  2:3335527/1‑93 (MQ=255)

                                                                     ccGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTcc                    >  1:2078136/1‑93 (MQ=255)

                                                                     ccGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTNANAGTAGCGAAANAAGCCTGTcc                    >  1:6932963/1‑93 (MQ=255)

                                                                      cGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCg                   <  1:5511025/93‑1 (MQ=255)

                                                                       gTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGt                  <  1:3935917/93‑1 (MQ=255)

                                                                        ttAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGtt                 >  1:7638937/1‑93 (MQ=255)

                                                                        ttAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGtt                 <  1:478260/93‑1 (MQ=255)

                                                                         tAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTa                >  1:5847688/1‑93 (MQ=255)

                                                                          aaTCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAg               <  2:3585025/93‑1 (MQ=255)

                                                                          aaTCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAg               <  1:6134456/93‑1 (MQ=255)

                                                                           aTCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAgg              >  1:2337290/1‑93 (MQ=255)

                                                                            tCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAggg             >  2:6344048/1‑93 (MQ=255)

                                                                             cTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGc            <  1:1067296/93‑1 (MQ=255)

                                                                             cTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGc            <  1:1939138/93‑1 (MQ=255)

                                                                               gCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTg          <  1:3810290/93‑1 (MQ=255)

                                                                               gCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGACTGTCCGTTAGGGcgg          >  1:1291429/1‑91 (MQ=255)

                                                                                  tCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCa       >  2:1145731/1‑93 (MQ=255)

                                                                                   cACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCAc      <  2:2035834/93‑1 (MQ=255)

                                                                                   cACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCAc      >  2:7565200/1‑93 (MQ=255)

                                                                                   cACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCAc      >  2:6454304/1‑93 (MQ=255)

                                                                                    aCCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCAcc     >  2:3468858/1‑93 (MQ=255)

                                                                                    aCCGGGC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGGGTTAAAGTATCGTAAGAAGCCTGTCCGTTAGGCGTGGCAcg     >  2:6593303/1‑92 (MQ=255)

                                                                                       ggTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGAc  >  2:55030/1‑93 (MQ=255)

GCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑CCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGAC  >  NC_000913/3339210‑3339388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`