BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,554,088	+C	coding (2/624 nt)	yhgKJ ←	RNA phosphage cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,554,088	1	.	C	99.3%	841.9 / ‑1.8	276	coding (2/624 nt)	yhgKJ	RNA phosphage cyclase
Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (119/153); total (120/156)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 323 total aligned reads displayed.

CGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT  >  NC_000913/3553996‑3554174

cGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCg                                                                                          >  1:2529814/1‑93 (MQ=255)

  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgca                                                                                        <  2:2007471/93‑3 (MQ=255)

  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgca                                                                                        >  1:1571286/1‑91 (MQ=255)

  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgca                                                                                        <  1:3131097/93‑3 (MQ=255)

  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgca                                                                                        <  2:5199264/93‑3 (MQ=255)

   cAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcag                                                                                       <  1:3481472/93‑4 (MQ=255)

   cAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcag                                                                                       <  2:3791241/93‑4 (MQ=255)

   cAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcag                                                                                       >  1:996005/1‑90 (MQ=255)

   cAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcag                                                                                       <  1:7639815/93‑4 (MQ=255)

    aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcagg                                                                                      <  1:4637534/93‑5 (MQ=255)

    aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcagg                                                                                      >  1:2067689/1‑89 (MQ=255)

    aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcagg                                                                                      >  1:7867292/1‑89 (MQ=255)

    aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcagg                                                                                      >  1:629414/1‑89 (MQ=255)

      ggTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAggcg                                                                                    >  2:6616310/1‑93 (MQ=255)

       gTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAggcgg                                                                                   >  1:818687/1‑93 (MQ=255)

        tAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAggcggg                                                                                  >  2:4006947/1‑92 (MQ=255)

          aaaaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGgcgc                                                                                <  1:4847479/93‑1 (MQ=255)

            aaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGCTAAAAT‑CCGCAGGCGGCGCCg                                                                              >  2:5891119/1‑93 (MQ=255)

             aCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGa                                                                             <  1:3322931/93‑1 (MQ=255)

              ccGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGAc                                                                            >  1:4063635/1‑93 (MQ=255)

              ccGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGAc                                                                            >  2:4364937/1‑93 (MQ=255)

                gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACgg                                                                          >  1:5875338/1‑93 (MQ=255)

                gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACgg                                                                          <  2:7108558/93‑1 (MQ=255)

                gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACgg                                                                          >  1:1862388/1‑93 (MQ=255)

                 tGGCGTANCAGCGTGGTTTGCNGNTGNATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACggg                                                                         <  1:3640573/93‑1 (MQ=255)

                 tGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGCTAAAAT‑CCGTAGGCGGCGCCGACggg                                                                         >  1:2626179/1‑93 (MQ=255)

                 tGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACggg                                                                         <  1:2279115/93‑1 (MQ=255)

                    cGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTa                                                                      <  2:4912845/93‑1 (MQ=255)

                      tAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATc                                                                    <  1:2985458/93‑1 (MQ=255)

                        aCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCgg                                                                  >  2:3105270/1‑93 (MQ=255)

                        aCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCgg                                                                  <  2:2382767/93‑1 (MQ=255)

                        aCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGAGGATAAAAT‑CCGCAGGCGGCGCCGATGGGTTATCgg                                                                  >  2:4183465/1‑93 (MQ=255)

                         cAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCggg                                                                 >  2:2828084/1‑92 (MQ=255)

                         cAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGt                                                                 <  1:3920346/93‑1 (MQ=255)

                          aGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtg                                                                <  2:6125962/93‑2 (MQ=255)

                           gCGTGGTTTGCTGATGAATTCCTATTTTCCCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtgc                                                               <  2:3418857/93‑3 (MQ=255)

                            cGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtgcc                                                              <  2:5952734/93‑4 (MQ=255)

                            cGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtgcc                                                              <  1:342853/93‑4 (MQ=255)

                             gTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtgccg                                                             >  1:6893634/1‑89 (MQ=255)

                              tGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGGTATCGGt‑gccg‑c                                                          >  2:3652426/1‑93 (MQ=255)

                               ggTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                         >  1:5820635/1‑92 (MQ=255)

                                 tttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccgc                                                       <  1:5705079/93‑4 (MQ=255)

                                  ttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACCGGGTATCGGt‑gccg‑ccgct                                                      >  1:3053362/1‑89 (MQ=255)

                                      tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACt                                                   >  2:3329543/1‑93 (MQ=255)

                                      tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAC‑CACCCGGCGGCTAAAAT‑GCGCAGGCAGCGCCCACGGGTTATCGGT‑GCCGCCGCTCACt                                                   >  2:1355230/1‑93 (MQ=255)

                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  1:6977695/1‑93 (MQ=255)

                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  2:545271/1‑93 (MQ=255)

                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  2:530499/1‑93 (MQ=255)

                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtc                                                  >  1:1440177/1‑92 (MQ=255)

                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGG‑GGCCGCCGCTCACtt                                                  >  2:6837683/1‑93 (MQ=255)

                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGAGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  2:1853502/1‑93 (MQ=255)

                                        atgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTc                                                 <  2:6971299/93‑1 (MQ=255)

                                        atgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTc                                                 >  2:6730897/1‑93 (MQ=255)

                                           aaTTCCTATGTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                              <  1:1914806/93‑1 (MQ=255)

                                            aTTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                             <  2:756923/93‑1 (MQ=255)

                                              tCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcg                                           >  1:2789640/1‑93 (MQ=255)

                                               ccTATTTTCGCCAGCAGCGGCTCCAGTCACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcg                                           <  2:6347659/93‑1 (MQ=255)

                                                cTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgcg                                         >  1:4324470/1‑93 (MQ=255)

                                                 tATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGa                                        <  2:4628966/93‑1 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  2:562508/1‑93 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      <  2:7653509/93‑1 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  1:2178055/1‑93 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  1:2501137/1‑93 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  2:7380892/1‑93 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  1:1773624/1‑93 (MQ=255)

                                                     ttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGt                                    <  2:1164765/93‑1 (MQ=255)

                                                     ttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGt                                    <  1:641154/93‑1 (MQ=255)

                                                      tCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTc                                   <  1:4915411/93‑1 (MQ=255)

                                                       cGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTcc                                  <  2:1261524/93‑1 (MQ=255)

                                                        gCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                 <  1:3766266/93‑1 (MQ=255)

                                                        gCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                 <  2:6170089/93‑1 (MQ=255)

                                                         ccAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTTAACACGCGAAGGTCCAt                                >  2:479386/1‑93 (MQ=255)

                                                         ccAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                >  1:5988828/1‑93 (MQ=255)

                                                         ccAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                <  1:4455036/93‑1 (MQ=255)

                                                          cagcagCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  2:2270082/93‑1 (MQ=255)

                                                          cagcagCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  1:5178464/93‑1 (MQ=255)

                                                          cagcagCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  1:7464618/93‑1 (MQ=255)

                                                          cagcagCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               >  2:1423817/1‑93 (MQ=255)

                                                          cagcagCGCCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  2:3028899/93‑1 (MQ=255)

                                                           agcagcGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                              <  2:6892954/93‑1 (MQ=255)

                                                           agcagcGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                              <  2:2724092/93‑1 (MQ=255)

                                                            gcagcGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                             >  2:1954379/1‑93 (MQ=255)

                                                                  gCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                       >  1:1280686/1‑93 (MQ=255)

                                                                   cTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACca                      <  2:494389/93‑1 (MQ=255)

                                                                   cTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACca                      <  2:392357/93‑1 (MQ=255)

                                                                    tCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcac                     >  2:1642190/1‑93 (MQ=255)

                                                                      cAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  2:778142/93‑1 (MQ=255)

                                                                      cAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  1:1967168/93‑1 (MQ=255)

                                                                      cAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  1:6736135/93‑1 (MQ=255)

                                                                      cAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  2:4246956/93‑1 (MQ=255)

                                                                       aG‑CACCCGGCGGATAAAAT‑CCNCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgc                  <  1:1063808/93‑1 (MQ=255)

                                                                          cgcccGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcgc                <  2:2216569/91‑1 (MQ=255)

                                                                           aCCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGcgcg               >  1:6159226/1‑93 (MQ=255)

                                                                            cccGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg              <  1:5243141/93‑1 (MQ=255)

                                                                            cccGACGGATAAAATACAC‑AGGCGGCGCCGACGGGTTATCGGAACCGC‑CGCTCACTTCAACACGCGAAGGTCCATCAGCAAACCACAGcacga              <  2:7037722/93‑2 (MQ=255)

                                                                              cggcggATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgc            <  2:6249280/93‑1 (MQ=255)

                                                                               ggcggATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgca           >  2:3577745/1‑93 (MQ=255)

                                                                               ggcggATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgca           >  1:4214801/1‑93 (MQ=255)

                                                                                   gATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAc       >  1:7816421/1‑93 (MQ=255)

                                                                                    aTAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAcc      >  2:5109746/1‑93 (MQ=255)

CGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT  >  NC_000913/3553996‑3554174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`