BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,575,446	+G	coding (912/942 nt)	gntR ←	GntR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,575,445	1	.	G	99.7%	1120.8 / ‑6.1	358	C305R (TGC→CGC)	gntR	GntR
Reads supporting (aligned to +/- strand): ref base . (0/1); major base G (214/141); minor base C (0/1); total (215/143)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 403 total aligned reads displayed.

ATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACC  >  NC_000913/3575353‑3575532

aTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                          <  1:7280516/93‑1 (MQ=255)

aTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                          <  2:7389032/93‑1 (MQ=255)

aTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                          <  2:6758011/93‑1 (MQ=255)

aTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                          <  2:3471283/93‑1 (MQ=255)

 tttGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑g                                                                                        >  2:7606974/1‑93 (MQ=255)

 tttGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑g                                                                                        >  1:3161191/1‑93 (MQ=255)

  ttGTGGGCTTAAATAGATCCGCCCGGTGCCAAGGTGAAACCTAAATCTAACATTTTCGGTGTCCCAGATTCGCCACGAATACGCGCcagca‑gg                                                                                       <  1:7017381/93‑2 (MQ=255)

       ggCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTc                                                                                   <  1:737327/93‑1 (MQ=255)

        gCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCa                                                                                  <  2:6275034/93‑1 (MQ=255)

        gCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCa                                                                                  <  1:5158532/93‑1 (MQ=255)

        gCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCa                                                                                  <  2:3427349/93‑1 (MQ=255)

          ttAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAgc                                                                                >  1:2791133/1‑93 (MQ=255)

          ttAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAgc                                                                                <  2:3615149/93‑1 (MQ=255)

          ttAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAgc                                                                                >  2:445188/1‑93 (MQ=255)

           tAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAgcg                                                                               >  1:453281/1‑93 (MQ=255)

            aaaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAgcgc                                                                              >  1:1025826/1‑93 (MQ=255)

             aaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGcc                                                                             >  2:4673843/1‑93 (MQ=255)

             aaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGcc                                                                             >  1:919834/1‑93 (MQ=255)

              aTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCa                                                                            >  2:2659222/1‑93 (MQ=255)

               tAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCaa                                                                           >  1:5546046/1‑93 (MQ=255)

               tAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCaa                                                                           >  1:5267987/1‑93 (MQ=255)

               tAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCaa                                                                           >  2:5603279/1‑93 (MQ=255)

                 gATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATa                                                                         <  1:3633581/93‑1 (MQ=255)

                  aTCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATAc                                                                        <  1:6889946/93‑1 (MQ=255)

                  aTCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATAc                                                                        <  1:7562298/93‑1 (MQ=255)

                  aTCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATAc                                                                        <  2:1035574/93‑1 (MQ=255)

                  aTCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATAc                                                                        <  2:1827009/93‑1 (MQ=255)

                    ccGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTg                                                                      <  2:4726863/93‑1 (MQ=255)

                     cGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGc                                                                     >  1:4909110/1‑93 (MQ=255)

                      gCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGcc                                                                    <  1:3762738/93‑1 (MQ=255)

                        ccGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCa                                                                  >  2:5999656/1‑93 (MQ=255)

                         cGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCAt                                                                 >  2:1222793/1‑93 (MQ=255)

                          ggTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATc                                                                <  2:2509282/93‑1 (MQ=255)

                               cAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTc                                                           >  2:5234130/1‑93 (MQ=255)

                               cAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTc                                                           >  1:2689873/1‑93 (MQ=255)

                                aaGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCa                                                          >  2:1323823/1‑93 (MQ=255)

                                aaGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCa                                                          >  2:4542963/1‑93 (MQ=255)

                                aaGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGCTCAGCGCCAATACTGACCATCCGCTCa                                                          >  1:1268212/1‑93 (MQ=255)

                                 aGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAc                                                         >  1:5070003/1‑93 (MQ=255)

                                   gTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcgc                                                       <  1:1152894/93‑1 (MQ=255)

                                   gTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcgc                                                       <  1:6685377/93‑1 (MQ=255)

                                   gTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcgc                                                       <  1:2168003/93‑1 (MQ=255)

                                   gTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcgc                                                       <  1:1649368/93‑1 (MQ=255)

                                    tGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcgcg                                                      <  1:2053535/93‑1 (MQ=255)

                                       aaCCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACgcggcg                                                   >  2:5433695/1‑93 (MQ=255)

                                        aCCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGt                                                  >  1:4250300/1‑93 (MQ=255)

                                         ccTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTc                                                 <  1:4379893/93‑1 (MQ=255)

                                          cTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCACGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCa                                                <  1:7652124/93‑1 (MQ=255)

                                             aaTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCa                                             <  1:6802305/93‑1 (MQ=255)

                                             aaTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCa                                             <  1:2951220/93‑1 (MQ=255)

                                             aaTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCa                                             <  2:2973977/93‑1 (MQ=255)

                                              aTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCAc                                            >  1:7912961/1‑93 (MQ=255)

                                                cTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGc                                          <  2:5499258/93‑1 (MQ=255)

                                                 tAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCt                                         >  2:6467872/1‑93 (MQ=255)

                                                 tAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCt                                         <  2:1446581/93‑1 (MQ=255)

                                                   aCATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCg                                       <  1:3387608/93‑1 (MQ=255)

                                                      ttttCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCCAGCGGCGTCAGCACGCTCGCag                                    >  2:6858179/1‑92 (MQ=255)

                                                      ttttCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCaa                                    >  2:7008427/1‑93 (MQ=255)

                                                      ttttCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCaa                                    >  2:7759042/1‑93 (MQ=255)

                                                       tttCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCCGTCAGCGCCAATCCTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAg                                   <  1:6574156/93‑1 (MQ=255)

                                                           ggTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGt                               <  1:6440094/93‑1 (MQ=255)

                                                           ggTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGt                               <  2:4317669/93‑1 (MQ=255)

                                                           ggTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGt                               >  2:3821723/1‑93 (MQ=255)

                                                           ggTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGt                               <  2:5911430/93‑1 (MQ=255)

                                                            gtgtCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTg                              >  1:5749289/1‑93 (MQ=255)

                                                             tgtCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTgg                             >  1:3856724/1‑93 (MQ=255)

                                                              gtCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGc                            >  2:2919558/1‑93 (MQ=255)

                                                               tCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCt                           >  1:4673716/1‑93 (MQ=255)

                                                               tCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCt                           >  1:5903805/1‑93 (MQ=255)

                                                               tCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCt                           >  2:6167692/1‑93 (MQ=255)

                                                               tCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCt                           >  1:5395384/1‑93 (MQ=255)

                                                                 acaGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTcc                         <  2:2001256/93‑1 (MQ=255)

                                                                 acaGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTcc                         >  2:1682593/1‑93 (MQ=255)

                                                                  caGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCa                        >  2:4979119/1‑93 (MQ=255)

                                                                   aGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCAt                       <  2:421452/93‑1 (MQ=255)

                                                                   aGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCAt                       >  2:7894600/1‑93 (MQ=255)

                                                                    gATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATc                      >  1:1788026/1‑93 (MQ=255)

                                                                    gATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATc                      >  2:875941/1‑93 (MQ=255)

                                                                     aTTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTACCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCa                     >  2:2353018/1‑93 (MQ=255)

                                                                         gCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTg                 >  2:7200326/1‑93 (MQ=255)

                                                                         gCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTg                 <  1:4106692/93‑1 (MQ=255)

                                                                          gcACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGa                <  1:2376048/92‑1 (MQ=255)

                                                                          ccACGAATACGCGCCAGCATGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGa                >  2:4825721/1‑93 (MQ=255)

                                                                          ccACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGa                >  1:4778213/1‑93 (MQ=255)

                                                                           cACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGTGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAc               >  2:5977160/1‑93 (MQ=255)

                                                                           cACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGAACGCTCGCAAGTCGTGGCTCCATCACCTGAc               >  2:7645245/1‑93 (MQ=255)

                                                                            aCGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAcc              >  1:2581599/1‑93 (MQ=255)

                                                                            aCGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAcc              >  2:5672052/1‑93 (MQ=255)

                                                                             cGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCa             >  2:214172/1‑93 (MQ=255)

                                                                               aaTACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAAt           >  2:3686898/1‑93 (MQ=255)

                                                                               aaTACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAAt           >  1:1582877/1‑93 (MQ=255)

                                                                                aTACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATg          >  2:3129295/1‑93 (MQ=255)

                                                                                aTACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATg          >  1:5638451/1‑93 (MQ=255)

                                                                                   cgcgCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCa       <  1:6028514/93‑1 (MQ=255)

                                                                                    gcgcCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCAt      <  2:7815024/93‑1 (MQ=255)

                                                                                     cgcCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATg     >  1:4006112/1‑93 (MQ=255)

                                                                                     cgcCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATg     <  1:3040757/93‑1 (MQ=255)

                                                                                       ccAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAc   >  1:6835123/1‑93 (MQ=255)

                                                                                        cagcagGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAcc  >  2:1926749/1‑93 (MQ=255)

                                                                                        cagcagGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAcc  >  1:1389872/1‑93 (MQ=255)

ATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACC  >  NC_000913/3575353‑3575532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`