BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,297	(C)_8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,289	1	.	C	90.8%	791.9 / 75.5	294	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (9/18); new base C (80/186); total (90/204)
Fisher's exact test for biased strand distribution p-value = 8.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGG  >  NC_000913/3980197‑3980379

 attattAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑c                                                                                           <  1:6626420/93‑1 (MQ=255)

 attattAGAAGTTGTGACAATGCGAAGGTGGCGGAATGGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑c                                                                                           <  1:3517836/93‑1 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑A‑‑‑cc                                                                                          >  1:7076422/1‑93 (MQ=255)

   tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑ccc                                                                                         <  1:5042998/93‑1 (MQ=255)

   tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑T‑‑‑ccc                                                                                         >  2:7174556/1‑93 (MQ=255)

    attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑cccc                                                                                        <  2:5969709/93‑1 (MQ=255)

      taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑cccccc                                                                                      <  1:3115272/93‑1 (MQ=255)

       aGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑ccccccc                                                                                     >  1:3166591/1‑93 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑cccccccc                                                                                    >  1:1680748/1‑93 (MQ=255)

         aaGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑ccccccccc                                                                                   <  2:3544197/93‑2 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑cccccccccc                                                                                  >  2:7670038/1‑91 (MQ=255)

           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑ccccccccctc                                                                                 <  1:2595070/93‑4 (MQ=255)

            ttGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑T‑‑‑tccccccccccc                                                                                >  2:2929454/1‑89 (MQ=14)

              ttgACAATGGGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGca                                                                               <  2:4022519/92‑1 (MQ=11)

                 aCAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGcacca                                                                            >  1:6384956/1‑93 (MQ=16)

                 aCAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGcacca                                                                            <  2:5072833/93‑1 (MQ=16)

                  cAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGcaccac                                                                           <  1:2347347/93‑1 (MQ=16)

                   aaTGCGAAGGTGGCGGAATTGGTAGGCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACg                                                                          <  1:1064226/93‑1 (MQ=255)

                    aTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGa                                                                         <  2:2179182/93‑1 (MQ=16)

                    aTGCGAAGGTGGCAGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGa                                                                         <  1:4026607/93‑1 (MQ=255)

                      gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑CCCCCCCCTCGCACCACGACtt                                                                      <  2:4925529/93‑1 (MQ=37)

                       ggAAGGGGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACtt                                                                      <  2:3064098/92‑1 (MQ=11)

                        gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACttt                                                                     <  2:1293166/93‑1 (MQ=255)

                        gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TA‑‑CCCCCCCCTCCCACCACGACttt                                                                     >  2:817038/1‑93 (MQ=255)

                        gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑GCCCCCCCTCGCCCCACGACTTTa                                                                    >  2:5398521/1‑93 (MQ=255)

                         aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGGCTTTa                                                                    >  2:6087277/1‑93 (MQ=255)

                           ggTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTaaa                                                                  <  1:1962269/93‑1 (MQ=255)

                           ggTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTaaa                                                                  <  1:4826702/93‑1 (MQ=255)

                            gTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAg                                                                 >  2:5233692/1‑93 (MQ=255)

                             tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGa                                                                <  2:6011446/93‑1 (MQ=255)

                              ggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGCC‑‑‑CCCCCCCCTCGCACCACGACTTTAAAGaa                                                               <  2:975495/93‑1 (MQ=255)

                              ggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TCC‑CCCCCCCCTCGCACCACGACTTTAAAGa                                                                <  2:1865664/93‑1 (MQ=14)

                              ggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGaa                                                               >  1:5428826/1‑93 (MQ=255)

                                cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAAtt                                                             <  2:4104021/93‑1 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGa                                                           <  1:2398198/93‑1 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGa                                                           <  1:5922703/93‑1 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGa                                                           >  1:3652180/1‑93 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGa                                                           <  2:1007885/93‑1 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGa                                                           >  1:6518190/1‑93 (MQ=255)

                                   aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGaa                                                          <  1:4302752/93‑1 (MQ=255)

                                   aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑TCC‑CCCCCCCCTCGCACCCCCACTTTAAAGAATTGa                                                           >  2:2098988/1‑93 (MQ=14)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCCCAACTTTAAAAAATTTAAc                                                         >  2:3265360/1‑93 (MQ=14)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAAc                                                         >  1:855076/1‑93 (MQ=255)

                                        gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTaaa                                                     <  1:382134/93‑1 (MQ=255)

                                        gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAAATaaa                                                     >  2:793309/1‑93 (MQ=255)

                                         tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGCCGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCCCCCCG‑CTTTTAAGAATTGAACTaaaaa                                                   <  1:1511878/93‑1 (MQ=14)

                                         tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaa                                                    <  1:445511/93‑1 (MQ=255)

                                          aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TCC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaa                                                    <  1:3277013/93‑1 (MQ=18)

                                           gACGCGCTAGCTTCAGGCGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TG‑‑CCCCTCCCTCGCACCCCGACTTTAAAGAATTGAACTAACAAt                                                  >  1:5242446/1‑93 (MQ=18)

                                            aCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAtt                                                 <  1:1013585/93‑1 (MQ=255)

                                             cgcgCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTc                                                >  2:2378024/1‑93 (MQ=255)

                                              gcgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCa                                               >  1:2040670/1‑93 (MQ=255)

                                               cgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaa                                              <  2:1509492/93‑1 (MQ=255)

                                               cgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaa                                              <  1:4693203/93‑1 (MQ=255)

                                               cgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaa                                              <  1:6438173/93‑1 (MQ=255)

                                                gcTAGCTTCAGGTGTTAGTGCCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaa                                             <  2:5280304/93‑1 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                            <  1:2894768/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                           <  2:4729691/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                           <  1:750153/93‑1 (MQ=255)

                                                   aGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑TC‑‑CCCCCCCCTCGCACCCCCACTTTTAAAAAATTAACTAAAAAATCaaaaaa                                          >  2:3208340/1‑92 (MQ=14)

                                                    gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGc                                         <  2:6395348/93‑1 (MQ=255)

                                                     cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                        <  1:6214247/93‑1 (MQ=255)

                                                      ttCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                       >  1:6861054/1‑93 (MQ=255)

                                                      ttCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                       >  2:3871069/1‑93 (MQ=255)

                                                      ttCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                       <  1:7904618/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAATTAAAAATTCAAAAAGCAGTa                                     >  1:1182732/1‑93 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                     >  2:752569/1‑93 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                     <  1:416794/93‑1 (MQ=255)

                                                         aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAt                                    <  2:6809099/93‑1 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TCCCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                     <  2:5531851/93‑1 (MQ=25)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                   <  2:2821741/93‑1 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                   <  2:1693531/93‑1 (MQ=255)

                                                           gtgtTAGTTTCCTTACGGATGTGGGGGTTCAAGCC‑‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                  <  2:6728047/93‑1 (MQ=37)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                  <  2:3651975/93‑1 (MQ=255)

                                                            tgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                 <  2:4329507/93‑1 (MQ=255)

                                                            tgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                 <  1:2776263/93‑1 (MQ=255)

                                                            tgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTAAAAAAGCAGTATTTc                                 <  2:6982956/93‑1 (MQ=25)

                                                            tgtTAGTCTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCCCCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                 <  1:2725750/93‑1 (MQ=37)

                                                             gtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                >  1:6778684/1‑93 (MQ=255)

                                                              ttAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                               <  2:6653615/93‑1 (MQ=255)

                                                                aGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCg                             <  2:4583844/93‑1 (MQ=255)

                                                                  tgtCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                           >  1:5442113/1‑93 (MQ=255)

                                                                  ggtCCTTACGGACGTGGGGGTTCAAG‑TCC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                            <  1:5715140/92‑1 (MQ=37)

                                                                    tCCTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                         <  2:5686668/93‑1 (MQ=255)

                                                                     ccTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                        <  1:7311268/93‑1 (MQ=255)

                                                                      cTTACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                       <  2:4437776/93‑1 (MQ=255)

                                                                      cTTACGGACGTGGGGGTTCAAG‑T‑‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAGATTCAAAAAGCAGTATTTCGGCGAGTAgcg                      >  2:7731212/1‑93 (MQ=255)

                                                                       ttACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcg                      >  1:3819884/1‑93 (MQ=255)

                                                                       ttACGGACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcg                      >  2:2569544/1‑93 (MQ=255)

                                                                            gACGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGGAGCGAAGCt                 >  1:6240417/1‑93 (MQ=38)

                                                                             gcGTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt                <  1:2524509/92‑1 (MQ=255)

                                                                               gTGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTgg              <  1:7668046/93‑1 (MQ=255)

                                                                                tGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGt             >  1:1386/1‑93 (MQ=255)

                                                                                tGGGGGTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGt             >  1:2101982/1‑93 (MQ=255)

                                                                                  ggggTTCAAG‑T‑‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGGGTAGCGCAGCTTGGTAgc          >  2:6213275/1‑93 (MQ=255)

                                                                                   gggTTCAAGCC‑‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgc          <  2:5756462/93‑1 (MQ=255)

                                                                                    ggTTCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgcg         <  2:2144116/93‑1 (MQ=255)

                                                                                       tCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa      <  2:7232522/93‑1 (MQ=255)

                                                                                       tCAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa      >  2:1346104/1‑93 (MQ=255)

                                                                                        cAAG‑TC‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc     <  1:2247651/93‑1 (MQ=255)

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGG  >  NC_000913/3980197‑3980379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`