Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,404 +G coding (487/516 nt) b4555 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4021.G97.9% 295.6 / ‑1.1 95coding (485/516 nt)b4555hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (35/58);  total (35/60)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGC‑‑‑G‑‑‑‑AAC‑T‑GG‑CTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTA  >  NC_000913/4008346‑4008481
                                                                   |                                                                                       
gcTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtt                                                          <  1:2469917/92‑1 (MQ=255)
 cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGttt                                                         <  1:6824227/93‑2 (MQ=255)
 cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGttt                                                         <  1:1059237/93‑2 (MQ=255)
 cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGttt                                                         <  2:5755912/93‑2 (MQ=255)
  tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtttg                                                        <  2:5036371/93‑3 (MQ=255)
  tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtttg                                                        <  1:6350927/93‑3 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtttgg                                                       <  1:176140/93‑4 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtttgg                                                       >  2:1261370/1‑90 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtttgg                                                       >  2:137114/1‑90 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtttgg                                                       <  1:1061422/93‑4 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtttgg                                                       <  1:251791/93‑4 (MQ=255)
    gAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtttggg                                                      >  1:2317758/1‑89 (MQ=255)
    gAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGtttggg                                                      >  2:5683019/1‑89 (MQ=255)
          aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                <  1:5170210/93‑1 (MQ=255)
          aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                >  2:3179687/1‑93 (MQ=255)
          aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                <  2:3772951/93‑1 (MQ=255)
          aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                >  2:502144/1‑93 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGa                                               <  1:3150267/93‑1 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGa                                               <  1:3177291/93‑1 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGa                                               <  1:7051633/93‑1 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGa                                               <  2:4595843/93‑1 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGa                                               <  1:2285960/93‑1 (MQ=255)
            ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGAt                                              <  2:5824838/93‑1 (MQ=255)
            ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGAt                                              <  2:4703577/93‑1 (MQ=255)
             gcgggcggGGTTTCAAAACGCCATGCGCTGGAAGCGGTGGCTAAGA‑‑‑AAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATg                                             <  2:2141291/93‑1 (MQ=255)
              cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGa                                            <  1:2852182/93‑1 (MQ=255)
               gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                           >  1:7618833/1‑93 (MQ=255)
               gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                           <  1:6162743/93‑1 (MQ=255)
               gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                           <  1:297305/93‑1 (MQ=255)
               gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                           <  2:4150200/93‑1 (MQ=255)
               gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                           <  1:6448749/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGGTGGAAGCGGTGGC‑‑‑GAAGAAACGG‑GG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                          <  1:7078414/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                          <  1:2454381/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                          <  1:7078413/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                          <  2:5450451/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                          >  2:7848379/1‑93 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                          >  1:2114222/1‑93 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGAGGAac                                          >  2:2476240/1‑93 (MQ=255)
                 gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacg                                         <  2:7488011/93‑1 (MQ=255)
                 gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacg                                         >  2:2387781/1‑93 (MQ=255)
                  cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                        <  2:184452/93‑1 (MQ=255)
                  cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                        <  1:1673796/93‑1 (MQ=255)
                  cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                        <  1:4611868/93‑1 (MQ=255)
                  cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                        >  1:4484584/1‑93 (MQ=255)
                  cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                        <  1:3005645/93‑1 (MQ=255)
                   ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                       <  1:6985594/93‑1 (MQ=255)
                   ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                       >  1:4823526/1‑93 (MQ=255)
                    gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacg                                      >  1:3319815/1‑93 (MQ=255)
                    gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacc                                      >  1:5719364/1‑92 (MQ=255)
                     cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGc                                     <  2:5618425/93‑1 (MQ=255)
                     cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGc                                     <  1:3574992/93‑1 (MQ=255)
                     cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGc                                     >  2:3399617/1‑93 (MQ=255)
                     cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GAATTGCGTTTGGTGACGGGATGAACGACGc                                     <  2:2986547/93‑1 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcg                                    >  1:1056564/1‑92 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcc                                    <  1:2079819/93‑1 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcc                                    <  2:6601284/93‑1 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcc                                    >  1:2358208/1‑93 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGCGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcg                                    >  2:5160899/1‑92 (MQ=255)
                        ttcaaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGa                                  <  1:2136365/89‑1 (MQ=255)
                        ttcaaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGa                                  <  2:4918077/89‑1 (MQ=255)
                         tcaaaaGGCCATGCGCTGGAAGTGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaa                                 >  1:1052812/4‑93 (MQ=255)
                         tcaaaaGGCCATGCGCTGGAAGTGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaa                                 >  1:4635586/4‑93 (MQ=255)
                         tcaaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaa                                 >  2:1515050/4‑93 (MQ=255)
                         tcaaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaa                                 <  2:6658377/90‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                <  1:5041729/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                >  1:553209/3‑93 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                >  1:3649935/3‑93 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                <  2:1472139/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                <  1:4433613/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                <  1:7277919/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                <  2:6859924/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                <  2:1777780/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                <  2:7352998/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                <  1:5401855/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                <  1:4920707/91‑1 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTTGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                               <  1:975371/92‑1 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GGAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACCACGCCGAAAt                               >  2:6936036/2‑93 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                               <  1:5409834/92‑1 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                               >  2:4664414/2‑93 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAAAGACGCCGAAAt                               >  2:3481560/2‑93 (MQ=255)
                            aaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGCCGCCGAAATg                              >  2:2449115/1‑93 (MQ=255)
                            aaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg                              >  1:7112816/1‑93 (MQ=255)
                            aaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg                              <  1:6346996/93‑1 (MQ=255)
                            aaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg                              >  2:3147096/1‑93 (MQ=255)
                            aaaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg                              >  1:6421733/1‑93 (MQ=255)
                             aaGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGc                             >  2:7165808/1‑93 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATTGG‑ATTGAGTTTGGGGACGGGATGAACGACGCCGAAGTGCt                            >  1:6774615/1‑93 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt                            >  2:4224177/1‑93 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt                            <  1:48196/93‑1 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt                            >  2:3129636/1‑93 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                           <  1:6027781/93‑1 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                           <  1:2850945/93‑1 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                           <  1:642075/93‑1 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGC‑‑‑GAAGAAAC‑TGGG‑CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                           <  1:4692973/93‑1 (MQ=255)
                                                           acaaaC‑T‑GGTCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTa  <  2:5612232/90‑1 (MQ=255)
                                                                   |                                                                                       
TCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGC‑‑‑G‑‑‑‑AAC‑T‑GG‑CTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTA  >  NC_000913/4008346‑4008481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑