Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 485,069 (G)5→6 coding (85/648 nt) acrR → acrAB operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913485,0641.G82.5% 699.2 / 156.2 268coding (80/648 nt)acrRacrAB operon repressor
Reads supporting (aligned to +/- strand):  ref base . (22/25);  new base G (117/104);  total (139/129)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 317 total aligned reads displayed.

AATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGA  >  NC_000913/484972‑485154
                                                                                             |                                                                                          
aaTTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca                                                                                             <  1:113185/93‑1 (MQ=255)
aaTTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca                                                                                             <  1:763771/93‑1 (MQ=255)
aaTTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca                                                                                             >  2:6382458/1‑93 (MQ=255)
aaTTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca                                                                                             >  2:2296582/1‑93 (MQ=255)
aaTTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca                                                                                             <  2:4383075/93‑1 (MQ=255)
 aTTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca‑g                                                                                           <  2:2225481/93‑1 (MQ=255)
  ttCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑gg                                                                                          <  1:5230227/93‑1 (MQ=255)
   tCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑ggg                                                                                         <  1:3158047/93‑1 (MQ=255)
   tCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑ggg                                                                                         <  2:7109949/93‑1 (MQ=255)
    cACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑gggg                                                                                        <  1:236522/93‑1 (MQ=255)
    cACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑gggg                                                                                        <  2:7307532/93‑1 (MQ=255)
     aCGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑ggggg                                                                                       <  2:3565428/93‑1 (MQ=255)
     aCGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑ggggg                                                                                       >  1:8848/1‑93 (MQ=255)
     aCGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑ggggg                                                                                       >  1:3747134/1‑93 (MQ=255)
       gAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑gggggca                                                                                     >  2:6024076/1‑91 (MQ=255)
        aaCATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑ggggggta                                                                                    <  2:1129739/93‑4 (MQ=255)
        aaCATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑GGGGGTat                                                                                    >  2:3917895/1‑93 (MQ=255)
         aCATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑ggggggtat                                                                                   >  1:5864649/1‑89 (MQ=255)
         aCATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑ggggggtat                                                                                   <  2:295458/93‑5 (MQ=255)
         aCATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAAAGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑ggggggtat                                                                                   >  1:3575994/1‑89 (MQ=255)
          aatatGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑GGGGGTatca                                                                                  <  1:6783131/92‑1 (MQ=255)
                  aCGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCt                                                                           >  2:3770835/1‑93 (MQ=255)
                    gAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCg                                                                         >  2:5958191/1‑93 (MQ=255)
                    gAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCg                                                                         >  2:2416707/1‑93 (MQ=255)
                    gAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCg                                                                         >  2:4889646/1‑93 (MQ=255)
                    gAAAAACCAAACAAGAAGAGCAAGAAACGCGCCAATACATCCTCGATATGGCTCTACGTATTTTCTCACAGAAGGGGGGTATCATCCACCtag                                                                         >  1:96627/1‑91 (MQ=255)
                     aaaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGc                                                                        <  2:1279684/93‑1 (MQ=255)
                     aaaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGc                                                                        >  1:7770551/1‑93 (MQ=255)
                     aaaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGc                                                                        >  1:599106/1‑93 (MQ=255)
                      aaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCt                                                                       >  1:4782186/1‑93 (MQ=255)
                      aaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCt                                                                       <  2:5081666/93‑1 (MQ=255)
                       aaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTgg                                                                     <  2:2586471/93‑1 (MQ=255)
                         aCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTggg                                                                    >  2:4494093/1‑93 (MQ=255)
                            aaaCAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCga                                                                 <  1:6953171/93‑1 (MQ=255)
                             aaCAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCgag                                                                >  2:6285680/1‑93 (MQ=255)
                              aCAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCgaga                                                               <  2:5564596/93‑1 (MQ=255)
                              aCAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCgaga                                                               <  2:7558845/93‑1 (MQ=255)
                              aCAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCgaga                                                               >  2:3847947/1‑93 (MQ=255)
                               cAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGAt                                                              <  2:6144076/93‑1 (MQ=255)
                               cAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGAt                                                              <  2:1956468/93‑1 (MQ=255)
                                  gaagCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGc                                                           >  1:3923302/1‑93 (MQ=255)
                                     gcgcAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCaaa                                                        <  1:1881681/93‑1 (MQ=255)
                                     gcgcAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCaaa                                                        >  1:2670887/1‑93 (MQ=255)
                                     gcgcAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCaaa                                                        >  1:7294208/1‑93 (MQ=255)
                                       gcAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAag                                                      >  1:7922049/1‑93 (MQ=255)
                                         aaGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAagca                                                    >  2:360809/1‑93 (MQ=255)
                                         aaGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAagca                                                    <  1:3029457/93‑1 (MQ=255)
                                         aaGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAagca                                                    <  1:6610411/93‑1 (MQ=255)
                                          aGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAagcag                                                   >  1:1260146/1‑93 (MQ=255)
                                            aaaCGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCt                                                 <  1:4333777/93‑1 (MQ=255)
                                             aaCGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTg                                                <  2:856247/93‑1 (MQ=255)
                                             aaCGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTg                                                >  1:3372365/1‑93 (MQ=255)
                                                  gcCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGtt                                           >  1:1011122/1‑93 (MQ=255)
                                                    cAACACGTCCTCTATGTGGCTCTACGGCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAc                                         <  1:1657238/93‑1 (MQ=255)
                                                    cAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAc                                         <  2:1569420/93‑1 (MQ=255)
                                                    cAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAc                                         <  1:630014/93‑1 (MQ=255)
                                                    cAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAc                                         >  1:5768789/1‑93 (MQ=255)
                                                     aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcg                                        >  2:49333/1‑93 (MQ=255)
                                                     aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcg                                        >  1:561814/1‑93 (MQ=255)
                                                     aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcg                                        >  1:1798459/1‑93 (MQ=255)
                                                     aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcg                                        >  2:6982760/1‑93 (MQ=255)
                                                     aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgc                                       >  2:3314047/1‑93 (MQ=255)
                                                       cacaTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                      >  1:492621/1‑93 (MQ=255)
                                                        acaTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                     >  1:4248918/1‑93 (MQ=255)
                                                          aTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCggg                                  >  1:5056882/1‑92 (MQ=255)
                                                           tCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGt                                  >  2:3348640/1‑93 (MQ=255)
                                                           tCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGt                                  >  1:4125731/1‑93 (MQ=255)
                                                           tCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTg                                 >  1:3226176/1‑93 (MQ=255)
                                                           tCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTCGCGTTACGCGCGGTg                                 >  1:7492694/1‑93 (MQ=255)
                                                            ccTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTg                                 <  1:717966/93‑1 (MQ=255)
                                                              tCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCa                               >  2:3694148/1‑93 (MQ=255)
                                                              tCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCa                               >  2:5501555/1‑93 (MQ=255)
                                                              tCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCa                               >  1:7121489/1‑93 (MQ=255)
                                                              tCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCa                               >  1:5814340/1‑93 (MQ=255)
                                                                  tgtgGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCt                           <  1:2052865/93‑1 (MQ=255)
                                                                  tgtgGCTCTACGTCTTTTCTCACAGCAGGGGGGGATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCt                           >  1:2502088/1‑93 (MQ=255)
                                                                  tgtgGCTCTACGTCTTTTCTCACAGCAGGGGGGCATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCt                           >  1:2273296/1‑93 (MQ=255)
                                                                    tgGCTCTGCGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTAc                         <  1:7456424/93‑1 (MQ=255)
                                                                    tgGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTAc                         <  2:4688986/93‑1 (MQ=255)
                                                                    tgGCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTAc                         >  2:2140932/1‑93 (MQ=255)
                                                                    tgGCTCTACGTCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACt                        <  1:4508893/93‑1 (MQ=255)
                                                                     ggCTCTGCGTCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTg                       <  2:465952/93‑1 (MQ=255)
                                                                     ggCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACt                        <  2:2808919/93‑1 (MQ=255)
                                                                      gCTCTACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTg                       <  2:7747630/93‑1 (MQ=255)
                                                                        tctACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGc                     >  2:1818323/1‑93 (MQ=255)
                                                                        tctACGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGc                     <  1:2949039/93‑1 (MQ=255)
                                                                           aCGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCAtt                  <  2:6685402/93‑1 (MQ=255)
                                                                           aCGTCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCAtt                  <  2:7387426/93‑1 (MQ=255)
                                                                           aCGTCTTTTCTCACAGCAGGGGGGCATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCAtt                  >  2:6877604/1‑93 (MQ=255)
                                                                              tCTTTTCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTa               >  1:6553051/1‑93 (MQ=255)
                                                                              tCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTaa              >  2:2792001/1‑93 (MQ=255)
                                                                              tCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTaa              >  2:6736384/1‑93 (MQ=255)
                                                                                ttgtctcACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTaaa             <  2:4138195/90‑1 (MQ=255)
                                                                                  ttCTCACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGa           <  2:1739289/93‑1 (MQ=255)
                                                                                   tctcACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGAc          <  1:210366/93‑1 (MQ=255)
                                                                                   tctcACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGAc          <  2:5291570/93‑1 (MQ=255)
                                                                                    ctcACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACaa        <  2:3249803/93‑1 (MQ=255)
                                                                                     tcACAGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACaa        >  1:2899402/1‑93 (MQ=255)
                                                                                       acaGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGt      <  1:3491416/93‑1 (MQ=255)
                                                                                       acaGCAGGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGt      >  1:2505730/1‑93 (MQ=255)
                                                                                             |                                                                                          
AATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCA‑GGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGA  >  NC_000913/484972‑485154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑