BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,448,541	+C	intergenic (+46/‑92)	ytfQ → / → ytfR	putative LACI‑type transcriptional regulator/YtfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,448,541	1	.	C	100.0%	731.3 / NA	258	intergenic (+46/‑92)	ytfQ/ytfR	putative LACI‑type transcriptional regulator/YtfR
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (152/106); total (152/106)

Only 100 of 293 total aligned reads displayed.

ATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCG‑‑G‑‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG  >  NC_000913/4448449‑4448623

 tACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCgc                                                                                       <  2:912985/93‑2 (MQ=255)

 tACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCgc                                                                                       >  1:124011/1‑92 (MQ=255)

 tACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCgc                                                                                       >  1:3386877/1‑92 (MQ=255)

 tACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGGTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCgc                                                                                       >  2:2124876/1‑92 (MQ=255)

            aagaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCgg                                                                          <  1:7236198/93‑1 (MQ=37)

            aagaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCgg                                                                          >  1:5232036/1‑93 (MQ=37)

             agaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGc                                                                         >  1:7341899/1‑93 (MQ=37)

              gaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCg                                                                        >  2:4724125/1‑93 (MQ=37)

              gaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCg                                                                        >  2:6483612/1‑93 (MQ=37)

               aaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGa                                                                       >  1:6002155/1‑93 (MQ=37)

                aTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGAt                                                                      <  1:2440038/93‑1 (MQ=37)

                 ttAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATg                                                                     >  2:5339996/1‑93 (MQ=37)

                 atAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATg                                                                     <  1:1854015/92‑1 (MQ=37)

                  tAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATgg                                                                    >  1:3062856/1‑93 (MQ=37)

                   agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATggg                                                                   >  2:3049534/1‑92 (MQ=37)

                    gagaAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTg                                                                  >  2:3076490/1‑93 (MQ=37)

                    gagaAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTg                                                                  >  1:555944/1‑93 (MQ=37)

                    gagaAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTg                                                                  >  2:7488105/1‑93 (MQ=37)

                    gagaAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTg                                                                  >  2:3682072/1‑93 (MQ=37)

                     agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGc                                                                 >  2:3758707/1‑93 (MQ=37)

                     agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGc                                                                 <  2:1083064/93‑1 (MQ=37)

                      gaagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCa                                                                >  2:597976/1‑93 (MQ=37)

                      gaagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCa                                                                >  2:2305972/1‑93 (MQ=37)

                      gaagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCa                                                                <  1:771801/93‑1 (MQ=37)

                       aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCAc                                                               <  1:3977068/93‑1 (MQ=37)

                        agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACt                                                              >  1:1038437/1‑93 (MQ=37)

                        agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACt                                                              <  1:3833497/93‑1 (MQ=37)

                        agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACt                                                              >  1:4602525/1‑93 (MQ=37)

                        agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACt                                                              <  1:2327270/93‑1 (MQ=37)

                        agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACt                                                              >  2:188809/1‑93 (MQ=37)

                         gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGCTAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTg                                                             >  1:2984298/1‑93 (MQ=37)

                           agaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGaa                                                           >  2:7926496/1‑93 (MQ=37)

                           agaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGaa                                                           <  1:6466417/93‑1 (MQ=37)

                             aaaaaTATGGGGTATTGAGGGTTGCTATGCCTGATCCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGc                                                         <  1:710522/93‑1 (MQ=25)

                              aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGcc                                                        >  1:7466711/1‑93 (MQ=37)

                              aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGcc                                                        <  2:5468116/93‑1 (MQ=37)

                              aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGcc                                                        <  2:6112765/93‑1 (MQ=37)

                              aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGcc                                                        <  1:2178163/93‑1 (MQ=37)

                               aaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCt                                                       <  1:3817306/93‑1 (MQ=37)

                                aaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTg                                                      >  2:1541898/1‑93 (MQ=37)

                                 atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGa                                                     <  2:5096756/93‑1 (MQ=37)

                                 atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGa                                                     >  1:4745802/1‑93 (MQ=37)

                                 atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGa                                                     <  2:4031006/93‑1 (MQ=37)

                                  tatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGAt                                                    >  1:7195309/1‑93 (MQ=37)

                                  tatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGAt                                                    >  2:6578295/1‑93 (MQ=37)

                                  tatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCCGCGATGGTGCACTGAAGCCTGAt                                                    >  2:4344637/1‑93 (MQ=37)

                                    tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                  >  1:7555510/1‑93 (MQ=25)

                                    tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                  >  2:3321444/1‑93 (MQ=25)

                                    tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                  >  2:6220307/1‑93 (MQ=25)

                                      gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCgc                                                >  2:1484466/1‑92 (MQ=25)

                                      gggTATGGGGGTTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAGGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGa                                                <  2:7086388/93‑1 (MQ=21)

                                            tGAGGGTTGCTTTGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTa                                          <  1:6696907/93‑1 (MQ=25)

                                            tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTa                                          >  1:2095654/1‑93 (MQ=25)

                                             gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc                                         <  2:3916901/93‑1 (MQ=25)

                                             gAGGGTTGCTATGCCTGATGCCGATGCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc                                         <  1:7786268/93‑1 (MQ=25)

                                              aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                        >  2:4525337/1‑93 (MQ=25)

                                              aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                        >  2:6048445/1‑93 (MQ=25)

                                               gggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcg                                       >  1:3219828/1‑93 (MQ=25)

                                                ggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                      <  1:6813432/93‑1 (MQ=25)

                                                ggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                      <  2:2424486/93‑1 (MQ=25)

                                                 gTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgcg                                     >  2:3388860/1‑93 (MQ=25)

                                                  ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt                                    >  2:3407024/1‑93 (MQ=25)

                                                  ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt                                    >  1:6172497/1‑93 (MQ=25)

                                                  ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt                                    >  2:7736296/1‑93 (MQ=25)

                                                    gCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt                                  <  1:926894/93‑1 (MQ=25)

                                                    gCTATGCCTGATGCCGATTCGTAGGCCGAATAAGGCGCCCGC‑GC‑CGCAGCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt                                  <  1:3144851/93‑1 (MQ=25)

                                                     cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCtt                                 >  1:3625221/1‑93 (MQ=25)

                                                      taggCCTGATGCGGTTTCGTAGGCGGGATAGGGCGCCCGC‑GC‑CGCACCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa                                <  2:6936004/90‑1 (MQ=21)

                                                      tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa                                >  1:3553549/1‑93 (MQ=21)

                                                      tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa                                >  2:7290185/1‑93 (MQ=21)

                                                       aTGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTAt                               <  1:6621791/93‑1 (MQ=25)

                                                        tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc                              >  1:4535941/1‑93 (MQ=21)

                                                         gCCTGATGCCGATTCGTAGGCCGGATAGGGCGCTCGCCC‑‑CGCATCGGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             <  2:1575048/93‑1 (MQ=21)

                                                         gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             <  2:4665575/93‑1 (MQ=21)

                                                         gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             <  1:6923308/93‑1 (MQ=21)

                                                         gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             <  2:6402489/93‑1 (MQ=25)

                                                            tGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc                          >  2:5750224/1‑93 (MQ=21)

                                                            tGATGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc                          >  1:1338147/1‑93 (MQ=21)

                                                               tGCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa                       >  2:5852204/1‑93 (MQ=21)

                                                                gCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTAc                      <  1:5809390/93‑1 (MQ=21)

                                                                gCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTAc                      <  1:5415723/93‑1 (MQ=18)

                                                                gCCGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTAc                      >  1:6233847/1‑93 (MQ=21)

                                                                 ccGATTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACt                     >  2:6365253/1‑93 (MQ=21)

                                                                  cGATGCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg                    <  2:674921/93‑1 (MQ=18)

                                                                    aTTCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                  >  2:6904323/1‑93 (MQ=21)

                                                                     ttCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGa                 <  2:2720901/93‑1 (MQ=21)

                                                                     ttCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGa                 <  1:3281777/93‑1 (MQ=21)

                                                                      tCGTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAg                <  1:399698/93‑1 (MQ=21)

                                                                        gTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCa              >  1:4349517/1‑93 (MQ=21)

                                                                        gTAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCa              <  1:740817/93‑1 (MQ=21)

                                                                         tAGGCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAc             <  2:3790887/93‑1 (MQ=21)

                                                                            gCCGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCt          <  2:742197/93‑1 (MQ=21)

                                                                              cGGATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCttt        <  1:1125834/93‑1 (MQ=21)

                                                                               ggATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTa       <  2:7625164/93‑1 (MQ=21)

                                                                                gATAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTac      >  2:78219/1‑93 (MQ=21)

                                                                                  tAAGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTacac    >  1:4133565/1‑93 (MQ=25)

                                                                                   aaGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACAcc   <  1:4945423/93‑1 (MQ=25)

                                                                                   aaGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACAcc   >  1:1719559/1‑93 (MQ=25)

                                                                                    aGGCGCTCGC‑GC‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCg  >  2:4267591/1‑93 (MQ=25)

                                                                                    aGGCGCTCG‑‑GCCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCg  <  1:149198/93‑1 (MQ=37)

ATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCG‑‑G‑‑CGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG  >  NC_000913/4448449‑4448623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`