BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	541,786	+G	coding (675/1146 nt)	ybbZ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	541,782	1	.	G	98.6%	685.3 / 0.3	221	coding (671/1146 nt)	ybbZ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (94/124); total (95/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 262 total aligned reads displayed.

CGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑GGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT  >  NC_000913/541690‑541869

cGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACa                                                                                          <  2:7026132/93‑1 (MQ=255)

cGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACa                                                                                          <  2:1382031/93‑1 (MQ=255)

  ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg                                                                                       >  2:5493406/1‑93 (MQ=255)

  ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg                                                                                       <  1:5616966/93‑1 (MQ=255)

  ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg                                                                                       <  1:4400319/93‑1 (MQ=255)

  ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg                                                                                       >  1:3775050/1‑93 (MQ=255)

  ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gg                                                                                       >  2:2911881/1‑93 (MQ=255)

   tGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑ggg                                                                                      <  2:718519/93‑1 (MQ=255)

    gATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gggg                                                                                     <  2:104695/93‑1 (MQ=255)

     aTAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑ggggg                                                                                    <  2:2325548/93‑2 (MQ=255)

      tAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gggggc                                                                                   <  1:2270322/93‑3 (MQ=255)

       aaCCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑gggggcg                                                                                  >  2:6754571/1‑90 (MQ=255)

            gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTTAACAGGGGGCTGCaaa                                                                              >  1:7046841/1‑93 (MQ=255)

            gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCaaa                                                                              >  2:6727016/1‑93 (MQ=255)

                gTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTa                                                                          <  1:2533504/93‑1 (MQ=255)

                gTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTa                                                                          <  1:1969550/93‑1 (MQ=255)

                   ggggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGc                                                                       <  1:1294289/93‑1 (MQ=255)

                   ggggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGc                                                                       >  1:149857/1‑93 (MQ=255)

                    gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGcc                                                                      <  2:6593780/93‑1 (MQ=255)

                    gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGcc                                                                      <  1:573471/93‑1 (MQ=255)

                    gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGcc                                                                      >  1:1669627/1‑93 (MQ=255)

                     ggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGccc                                                                     <  1:905178/93‑1 (MQ=255)

                     ggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGccc                                                                     <  2:6149079/93‑1 (MQ=255)

                       cACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCgt                                                                   <  2:3586414/93‑1 (MQ=255)

                        aCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCgtg                                                                  >  2:4206210/1‑93 (MQ=255)

                          gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGtt                                                                >  1:206245/1‑93 (MQ=255)

                            ggcgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGtttt                                                              >  1:7917415/1‑93 (MQ=255)

                            ggcgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGtttt                                                              <  2:7451618/93‑1 (MQ=255)

                               gcggcggcgGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTaca                                                           <  1:672978/93‑1 (MQ=255)

                                cggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTacaa                                                          <  2:5663544/93‑1 (MQ=255)

                                 ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTacaac                                                         <  1:1809787/93‑1 (MQ=255)

                                   cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAg                                                       <  2:2167425/93‑1 (MQ=255)

                                    ggcggTGTTTGTCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGc                                                      <  2:2217652/93‑1 (MQ=255)

                                       ggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCaaa                                                   <  1:4707860/93‑1 (MQ=255)

                                       ggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCaaa                                                   <  2:1478316/93‑1 (MQ=255)

                                       ggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCaaa                                                   >  2:5753370/1‑93 (MQ=255)

                                        gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                  >  1:4573135/1‑93 (MQ=255)

                                        gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                  <  1:3518884/93‑1 (MQ=255)

                                        gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                  <  1:6854815/93‑1 (MQ=255)

                                        gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGGGTTTTACAACAGCCAAc                                                  >  2:51982/1‑93 (MQ=255)

                                          gtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                                >  1:939342/1‑93 (MQ=255)

                                          gtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                                >  2:2066865/1‑93 (MQ=255)

                                          gtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                                >  1:104082/1‑93 (MQ=255)

                                           tttGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGa                                               >  1:2995185/1‑93 (MQ=255)

                                           tttGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGa                                               >  2:7512567/1‑93 (MQ=255)

                                           tttGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGa                                               >  2:4063351/1‑93 (MQ=255)

                                             tGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                             >  1:6180798/1‑93 (MQ=255)

                                             tGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                             <  2:3673278/93‑1 (MQ=255)

                                             tGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                             <  1:5085005/93‑1 (MQ=255)

                                              ggCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGTAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAAt                                            >  1:6627894/1‑93 (MQ=255)

                                               gCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaagt                                           <  2:4700371/93‑3 (MQ=255)

                                               gCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAAtt                                           >  2:5900754/1‑93 (MQ=255)

                                                  cGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAAt                                        <  1:6465995/93‑1 (MQ=255)

                                                    cAAAAAGGGGCATCGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGt                                      <  1:1623584/93‑1 (MQ=255)

                                                     aaaaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTc                                     <  2:855592/93‑1 (MQ=255)

                                                      aaaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCt                                    >  1:7317349/1‑93 (MQ=255)

                                                       aaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTg                                   <  1:6437613/93‑1 (MQ=255)

                                                          ggggCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCa                                >  1:1550529/1‑93 (MQ=255)

                                                           gggCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAg                               <  1:3751090/93‑1 (MQ=255)

                                                            ggCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                              <  1:6556844/93‑1 (MQ=255)

                                                            ggCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                              <  1:7386547/93‑1 (MQ=255)

                                                             gCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                             <  2:7537858/93‑1 (MQ=255)

                                                             gCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                             <  2:2851572/93‑1 (MQ=255)

                                                              cAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATg                            <  2:13583/93‑1 (MQ=255)

                                                              cAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATg                            >  2:631610/1‑93 (MQ=255)

                                                              cAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATg                            <  2:4492172/93‑1 (MQ=255)

                                                               aaCGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATgg                           <  1:7890568/93‑1 (MQ=255)

                                                                aCGCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggc                          >  1:4066070/1‑93 (MQ=255)

                                                                  gCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgg                        <  2:943769/93‑1 (MQ=255)

                                                                  gCCGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgg                        >  1:3229228/1‑93 (MQ=255)

                                                                   ccGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcggg                       <  2:1172733/93‑1 (MQ=255)

                                                                   ccGGAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcggg                       >  2:5555546/1‑93 (MQ=255)

                                                                     ggAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgggcg                     <  1:262717/93‑1 (MQ=255)

                                                                     ggAGATGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgggcg                     >  1:4260374/1‑93 (MQ=255)

                                                                      gagaTGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                    >  1:225149/1‑93 (MQ=255)

                                                                      gagaTGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                    >  1:7655071/1‑93 (MQ=255)

                                                                      gagaTGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                    <  1:6913145/93‑1 (MQ=255)

                                                                      gagaTGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                    >  1:6480540/1‑93 (MQ=255)

                                                                       agaTGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggc                   <  2:1411293/93‑1 (MQ=255)

                                                                        gaTGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcg                  >  1:4689838/1‑93 (MQ=255)

                                                                        gaTGGTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcg                  >  1:6149092/1‑93 (MQ=255)

                                                                           ggTCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGgcggcggcg               <  2:24012/93‑1 (MQ=255)

                                                                             tCGAAGAACTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCt             <  2:6077308/93‑1 (MQ=255)

                                                                               gaagaaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgc           <  1:5662532/93‑1 (MQ=255)

                                                                                aagaaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg          <  1:5414351/93‑1 (MQ=255)

                                                                                aagaaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg          >  1:522261/1‑93 (MQ=255)

                                                                                aagaaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg          >  2:1206958/1‑93 (MQ=255)

                                                                                aagaaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg          >  1:6111791/1‑93 (MQ=255)

                                                                                 agaaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg         >  1:6023316/1‑93 (MQ=255)

                                                                                 agaaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg         >  2:3862449/1‑93 (MQ=255)

                                                                                  gaaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg        >  1:4197295/1‑93 (MQ=255)

                                                                                  gaaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg        >  2:1640975/1‑93 (MQ=255)

                                                                                   aaCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc       <  1:2426835/93‑1 (MQ=255)

                                                                                    aCTTGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggcg      <  2:2525883/93‑1 (MQ=255)

                                                                                      ttGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt    <  1:100593/93‑1 (MQ=255)

                                                                                      ttGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt    >  2:312034/1‑93 (MQ=255)

                                                                                      ttGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt    >  2:7840212/1‑93 (MQ=255)

                                                                                      ttGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt    >  2:4825427/1‑93 (MQ=255)

                                                                                       tGAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTa   <  2:7229194/93‑1 (MQ=255)

                                                                                        gAACAGGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt  <  1:4352473/93‑1 (MQ=255)

CGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACA‑GGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT  >  NC_000913/541690‑541869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`