BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	547,835	+G	coding (255/261 nt)	b4507 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	547,831	1	.	G	99.2%	805.6 / ‑1.6	261	coding (251/261 nt)	b4507	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (145/114); total (145/116)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 282 total aligned reads displayed.

GATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTG  >  NC_000913/547739‑547918

  tGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg                                                                                       <  2:5461212/93‑1 (MQ=255)

   gACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg                                                                                      >  1:3109408/1‑93 (MQ=255)

      cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGGAGCGCAGGCGCTGGCAATACTGGAGCA‑ggggga                                                                                   >  1:4517314/1‑91 (MQ=255)

      cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggggga                                                                                   >  2:526980/1‑91 (MQ=255)

        gACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gggggaag                                                                                 >  2:6297664/1‑89 (MQ=255)

             aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAAc                                                                             >  2:2772701/1‑93 (MQ=255)

             aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAAc                                                                             <  1:6459695/93‑1 (MQ=255)

              tGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACt                                                                            >  1:4227575/1‑93 (MQ=255)

               gAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt                                                                           >  2:2034129/1‑93 (MQ=255)

               gAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt                                                                           >  1:5266763/1‑93 (MQ=255)

                   ggggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCAtt                                                                       <  1:1967264/93‑1 (MQ=255)

                   ggggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCAtt                                                                       >  2:4744028/1‑93 (MQ=255)

                    gggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTTAACTTCATTc                                                                      <  1:4005572/93‑1 (MQ=255)

                     ggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTcc                                                                     >  1:4428798/1‑93 (MQ=255)

                      gcgtgcgtGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCt                                                                    <  1:7769738/93‑1 (MQ=255)

                       cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                                                   <  2:3547913/93‑1 (MQ=255)

                       cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                                                   <  2:453929/93‑1 (MQ=255)

                       cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                                                   <  2:7420973/93‑1 (MQ=255)

                        gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTg                                                                  >  2:7139405/1‑93 (MQ=255)

                        gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTg                                                                  <  2:5283374/93‑1 (MQ=255)

                            gtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAc                                                              >  2:3182761/1‑93 (MQ=255)

                             tgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc                                                             >  2:442803/1‑93 (MQ=255)

                              gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGTAGGGGGAAGTGAACTTAATTCCTTGTCACCa                                                            <  1:6551178/93‑1 (MQ=255)

                              gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa                                                            <  2:3736104/93‑1 (MQ=255)

                                 cgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgt                                                         >  1:4757730/1‑93 (MQ=255)

                                  gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                                        >  1:7103231/1‑93 (MQ=255)

                                  gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                                        >  2:7219081/1‑93 (MQ=255)

                                  gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                                        >  2:6048004/1‑93 (MQ=255)

                                  gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                                        >  1:6067818/1‑93 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCTTGTGaa                                                      <  2:7680597/93‑1 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                      <  2:805005/93‑1 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                      <  2:5677096/93‑1 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                      <  1:1311093/93‑1 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                      <  1:5846954/93‑1 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                      >  2:7879214/1‑93 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                      <  2:6195066/93‑1 (MQ=255)

                                     tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTTATTCCTTGTCACCATGTGAAt                                                     >  2:6860467/1‑93 (MQ=255)

                                     tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                                                     >  1:2031571/1‑93 (MQ=255)

                                     tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                                                     >  2:1252360/1‑93 (MQ=255)

                                      ttCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg                                                    <  1:6706699/92‑1 (MQ=255)

                                      gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg                                                    >  2:6962245/1‑93 (MQ=255)

                                       tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTTACCATGTGAATGc                                                   >  2:2391463/1‑93 (MQ=255)

                                        cTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc                                                  >  1:6242799/1‑93 (MQ=255)

                                         tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg                                                 >  1:598005/1‑93 (MQ=255)

                                         tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg                                                 >  1:3403011/1‑93 (MQ=255)

                                          gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATgaagt                                                >  1:7642625/1‑89 (MQ=255)

                                           ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                               >  1:976140/1‑93 (MQ=255)

                                           ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                               <  2:7552129/93‑1 (MQ=255)

                                           ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                               >  1:5030804/1‑93 (MQ=255)

                                           ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTACTTGTCACCATGTGAATGCCGTc                                               >  2:5655687/1‑93 (MQ=255)

                                             cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                                             <  1:1443501/93‑1 (MQ=255)

                                             cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                                             <  2:1929306/93‑1 (MQ=255)

                                              gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg                                            >  2:3073292/1‑93 (MQ=255)

                                              gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTAGTCACCATGTGAATGCCGTCggg                                            >  2:4287971/1‑93 (MQ=255)

                                              gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCC‑TGTCACCATGTGAATGCCGTCGGGc                                           <  1:2723014/93‑1 (MQ=255)

                                              gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGCGAACTTCATTCCTTGTCACCATGGCAATGCCGTCggg                                            >  2:929833/1‑93 (MQ=255)

                                               aaGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                                           >  1:5759476/1‑93 (MQ=255)

                                                aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                                          >  2:3463120/1‑93 (MQ=255)

                                                 ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa                                         >  1:2119209/1‑93 (MQ=255)

                                                 ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa                                         <  1:6124433/93‑1 (MQ=255)

                                                 ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa                                         >  2:4059116/1‑93 (MQ=255)

                                                   ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt                                       <  2:645454/93‑1 (MQ=255)

                                                    tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg                                      >  1:6909110/1‑93 (MQ=255)

                                                    tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg                                      <  1:5504682/93‑1 (MQ=255)

                                                     gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg                                     >  1:1051764/1‑93 (MQ=255)

                                                      ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGCCGGCCCAATggg                                    >  1:6801520/1‑93 (MQ=255)

                                                       gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc                                   <  1:243566/93‑1 (MQ=255)

                                                        cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg                                  <  1:2901478/93‑1 (MQ=255)

                                                           aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTa                               <  1:1926726/93‑1 (MQ=255)

                                                            aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCACTGTGCGGTAt                              >  2:5097063/1‑93 (MQ=255)

                                                            aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt                              <  1:3680821/93‑1 (MQ=255)

                                                            aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt                              >  2:5347688/1‑93 (MQ=255)

                                                             gATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAtt                             >  1:660968/1‑93 (MQ=255)

                                                              aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTa                            <  1:1623421/93‑1 (MQ=255)

                                                              aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTa                            >  2:256991/1‑93 (MQ=255)

                                                               tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc                           <  1:4642224/93‑1 (MQ=255)

                                                               tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc                           >  2:6335146/1‑93 (MQ=255)

                                                               ataaGCGCAGGCGCTGGCAATAGTGGAGCAGGGGGAAGTGACCATCATTCCTTGTAACCATGTGAATGCCGTCGTGCCAATGGGCGGTATTAc                           <  2:1343945/91‑1 (MQ=255)

                                                                gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt                          <  2:1682359/93‑1 (MQ=255)

                                                                gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt                          >  1:3498277/1‑93 (MQ=255)

                                                                       aGGCGCTGGCAATACTGGAGCAGGGGGCAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTCCCa                   >  2:6535025/1‑93 (MQ=255)

                                                                        ggCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCTTCCTGGGAATGCCGTCGGGCCAATGCGCGTTATTACTTCTGCCat                  >  2:6764330/1‑92 (MQ=255)

                                                                        ggCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg                  >  2:7389713/1‑93 (MQ=255)

                                                                        ggCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg                  >  2:2622249/1‑93 (MQ=255)

                                                                          cacTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTa                <  2:3850136/91‑1 (MQ=255)

                                                                            cTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATg              >  2:557657/1‑93 (MQ=255)

                                                                             tGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGc             <  1:7054784/93‑1 (MQ=255)

                                                                              ggCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGcc            <  2:2238555/93‑1 (MQ=255)

                                                                                cAATACTGGAGTAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGa          >  1:1876075/1‑93 (MQ=255)

                                                                                cAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGa          >  2:4176736/1‑93 (MQ=255)

                                                                                 aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGAt         >  1:7149565/1‑93 (MQ=255)

                                                                                 aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGAt         <  2:2876695/93‑1 (MQ=255)

                                                                                 aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGAt         <  1:3438936/93‑1 (MQ=255)

                                                                                  aTACTGGAGTAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATg        <  2:5909593/93‑1 (MQ=255)

                                                                                  aTACTGGAGTAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATg        <  1:4046219/93‑1 (MQ=255)

                                                                                  aTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATg        >  1:3340743/1‑93 (MQ=255)

                                                                                  aTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGCATTACTTCTGCCAGTATGCCGATg        >  2:6143474/1‑93 (MQ=255)

                                                                                   tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGc       >  1:1116704/1‑93 (MQ=255)

                                                                                   aaCTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGc       <  2:7778444/92‑1 (MQ=255)

                                                                                    aCTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCt      >  1:7612508/1‑93 (MQ=255)

GATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTG  >  NC_000913/547739‑547918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`