BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	955,785	+A	coding (71/1761 nt)	ycaO ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	955,782	1	.	A	98.7%	821.5 / 1.6	225	coding (74/1761 nt)	ycaO	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/1); new base A (102/120); total (104/121)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

ACAGTGCGCACTCTTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑G‑AAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAG  >  NC_000913/955690‑955869

 caGTGCGCACTCTTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑G‑a                                                                                        >  2:2793238/1‑93 (MQ=255)

  aGTGCGCACTCTTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑G‑aa                                                                                       <  2:157104/93‑1 (MQ=255)

   gTGCGCACTCTTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑G‑aaa                                                                                      >  2:6784404/1‑93 (MQ=255)

       gcACTCTTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑G‑aaaagtt                                                                                  >  2:5318173/1‑89 (MQ=255)

         aCTCTTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑G‑AAAAgtttt                                                                                <  2:7279115/93‑1 (MQ=255)

         aCTCTTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑G‑AAAAgtttt                                                                                >  2:1669439/1‑93 (MQ=255)

          ctctTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGttttt                                                                                <  1:7137986/93‑1 (MQ=255)

           tctTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCTGG‑AAAGTTTTTg                                                                               >  2:3229221/1‑93 (MQ=255)

           tctTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTg                                                                               <  1:3507421/93‑1 (MQ=255)

            ctTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGc                                                                              <  2:6188204/93‑1 (MQ=255)

             tttGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCt                                                                             <  1:2194392/93‑1 (MQ=255)

             tttGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCt                                                                             <  2:7597456/93‑1 (MQ=255)

             tttGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCt                                                                             <  1:1202053/93‑1 (MQ=255)

              ttGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTg                                                                            >  1:6531320/1‑93 (MQ=255)

              ttGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTg                                                                            >  2:4659797/1‑93 (MQ=255)

               tGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTgg                                                                           >  2:4741704/1‑93 (MQ=255)

                gTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGa                                                                          >  1:2917572/1‑93 (MQ=255)

                gTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGa                                                                          >  2:2566881/1‑93 (MQ=255)

                  cgcgAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAag                                                                        <  1:7597695/93‑1 (MQ=255)

                   gctaaTATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAagc                                                                       <  1:1286948/90‑1 (MQ=255)

                      aaTATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAagcgag                                                                    >  2:767698/1‑93 (MQ=255)

                       atatGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAagcgagc                                                                   <  1:5437138/93‑1 (MQ=255)

                        tatGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAagcgagcg                                                                  >  1:4092411/1‑93 (MQ=255)

                         atGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAgcgagcga                                                                 <  2:3137234/93‑1 (MQ=255)

                          tGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAgcgagcgag                                                                >  1:3876923/1‑92 (MQ=255)

                          tGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGAt                                                                <  1:188583/93‑1 (MQ=255)

                           gTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATg                                                               <  2:1153992/93‑1 (MQ=255)

                           gTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATg                                                               <  2:6439666/93‑1 (MQ=255)

                           gTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGGTTTTGCTGGAAGCGAGCGCTg                                                               >  2:6666058/1‑93 (MQ=255)

                            tACAGACCAGGCGTTAGGCACGGGCTTCAGACAGGAGGCCCCTTCAATCTGACAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCATCCGATgg                                                              >  2:5401885/1‑93 (MQ=255)

                             aCAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGa                                                             <  2:3491374/93‑1 (MQ=255)

                              cagaccagacGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGaa                                                            <  1:7777022/93‑1 (MQ=255)

                              cagaccagacGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGaa                                                            <  2:4866149/93‑1 (MQ=255)

                               agaccagacGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAAt                                                           <  2:2126129/93‑1 (MQ=255)

                               agaccagacGTTAGGCACGGGATTCAACCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAAt                                                           >  1:4908061/1‑93 (MQ=255)

                                gaccagacGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATc                                                          <  2:7488099/93‑1 (MQ=255)

                                 accagacGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCt                                                         <  2:4962944/93‑1 (MQ=255)

                                  ccagacGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCtt                                                        >  1:5927350/1‑93 (MQ=255)

                                   cagacGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTc                                                       <  2:2877229/93‑1 (MQ=255)

                                        gTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCagag                                                  <  1:3732688/93‑1 (MQ=255)

                                         ttAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGc                                                 <  1:6298864/93‑1 (MQ=255)

                                         ttAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGc                                                 <  2:7839658/93‑1 (MQ=255)

                                         ttAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGc                                                 >  2:7177790/1‑93 (MQ=255)

                                         ttAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGc                                                 >  2:1053957/1‑93 (MQ=255)

                                         ttAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGCAATCTTCCAGAGc                                                 >  2:3996573/1‑93 (MQ=255)

                                            ggCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCgga                                              >  2:285100/1‑92 (MQ=255)

                                            ggCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGc                                              <  1:2206225/93‑1 (MQ=255)

                                            ggCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGc                                              >  2:231418/1‑93 (MQ=255)

                                            ggCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGc                                              >  1:6793757/1‑93 (MQ=255)

                                             gCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCa                                             <  2:3883637/93‑1 (MQ=255)

                                              cACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCAt                                            >  1:5134792/1‑93 (MQ=255)

                                               aCGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATc                                           >  2:4566140/1‑93 (MQ=255)

                                               aCGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATc                                           <  1:6657542/93‑1 (MQ=255)

                                               aCGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATc                                           <  1:978156/93‑1 (MQ=255)

                                                cGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCt                                          <  1:7276186/93‑1 (MQ=255)

                                                cGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCt                                          >  1:867264/1‑93 (MQ=255)

                                                 gggATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCtt                                         <  2:3601219/93‑1 (MQ=255)

                                                  ggATTCAGCCAGGAGGCCTCTNCNATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCttt                                        <  1:2601633/93‑1 (MQ=255)

                                                   gATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTg                                       <  1:1227705/93‑1 (MQ=255)

                                                   gATTCAGCCAGAAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTg                                       >  2:7800251/1‑93 (MQ=255)

                                                     ttCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGcc                                     <  1:3659700/93‑1 (MQ=255)

                                                     ttCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGcc                                     <  1:4536192/93‑1 (MQ=255)

                                                         gCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCgggg                                 >  1:1775154/1‑93 (MQ=255)

                                                           cAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGAt                               <  1:1068344/93‑1 (MQ=255)

                                                           cAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGAt                               <  1:3103289/93‑1 (MQ=255)

                                                           cAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGAt                               >  2:5908854/1‑93 (MQ=255)

                                                           cAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGAt                               <  1:480541/93‑1 (MQ=255)

                                                              gaggCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATaaa                            <  2:877386/93‑1 (MQ=255)

                                                                 gCCTCTTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGt                         >  1:7499115/1‑93 (MQ=255)

                                                                   ctctTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGttt                       <  2:6399493/93‑1 (MQ=255)

                                                                   ctctTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGttt                       <  2:5266557/93‑1 (MQ=255)

                                                                   ctctTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGttt                       <  2:7908936/93‑1 (MQ=255)

                                                                    tctTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTg                      >  1:7910002/1‑93 (MQ=255)

                                                                    tctTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTg                      >  1:5325895/1‑93 (MQ=255)

                                                                    tctTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTg                      >  1:6059738/1‑93 (MQ=255)

                                                                     ctTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGc                     >  2:951577/1‑93 (MQ=255)

                                                                     ctTCAATCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGc                     <  1:5787377/93‑1 (MQ=255)

                                                                         aaTCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCa                 <  1:1424675/93‑1 (MQ=255)

                                                                          aTCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCAt                <  1:5170022/93‑1 (MQ=255)

                                                                          aTCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCAt                >  1:3978062/1‑93 (MQ=255)

                                                                           tCTGAAAGCCGGGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATg               >  2:3695105/1‑93 (MQ=255)

                                                                           tCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATg               <  2:4206150/93‑1 (MQ=255)

                                                                           tCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATg               <  1:3632311/93‑1 (MQ=255)

                                                                           tCTGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATg               >  2:2723346/1‑93 (MQ=255)

                                                                             tGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGaa             <  1:2752765/93‑1 (MQ=255)

                                                                             tGAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGaa             >  2:6355719/1‑93 (MQ=255)

                                                                              gAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGaaa            >  1:5310616/1‑93 (MQ=255)

                                                                              gAAAGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGaaa            >  2:4424965/1‑93 (MQ=255)

                                                                               aaaGCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGaaaa           <  1:3892218/93‑1 (MQ=255)

                                                                                  gCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCa        <  1:3327511/93‑1 (MQ=255)

                                                                                  gCCGAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCa        <  1:6700581/93‑1 (MQ=255)

                                                                                     gAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACtt     >  2:7714107/1‑93 (MQ=255)

                                                                                     gAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACtt     <  1:3198005/93‑1 (MQ=255)

                                                                                     gAGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACtt     >  1:2511237/1‑93 (MQ=255)

                                                                                      aGGTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACttt    >  1:6097025/1‑93 (MQ=255)

                                                                                       ggTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTa   <  1:4567578/93‑1 (MQ=255)

                                                                                       ggTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTa   <  1:7685237/93‑1 (MQ=255)

                                                                                        gTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAg  <  1:4539944/93‑1 (MQ=255)

                                                                                        gTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAg  >  1:3727535/1‑93 (MQ=255)

                                                                                        gTCT‑GAAAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAg  >  2:5682734/1‑93 (MQ=255)

ACAGTGCGCACTCTTTGTCGCGAATATGTACAGACCAGACGTTAGGCACGGGATTCAGCCAGGAGGCCTCTTCAATCTGAAAGCCGAGGTCT‑G‑AAAGTTTTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAG  >  NC_000913/955690‑955869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`