Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,096,622	(G)7→8	intergenic (+570/+166)	ycdU → / ← serX	hypothetical protein/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,096,615	1	.	G	93.8%	28.1 / ‑2.7	16	intergenic (+563/+173)	ycdU/serX	hypothetical protein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (5/10);  total (6/10)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCG  >  NC_000913/1096536‑1096636                                                                                 |                      ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGa           >  1:4520341/1‑93 (MQ=0) ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGa           <  2:4457588/93‑1 (MQ=0)  tctcATCCCCCCGGTGTGTGCCATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGAc          >  2:3705512/1‑93 (MQ=255)  tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTGaac         >  2:125568/1‑91 (MQ=255)   ctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACt         <  1:2732024/93‑1 (MQ=255)    tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtc        >  1:5653729/1‑93 (MQ=255)      aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgc      >  1:5131560/1‑93 (MQ=255)      aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGACtcgc      >  2:2990941/1‑93 (MQ=11)      aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgc      >  1:3253857/1‑93 (MQ=255)      aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgc      <  1:540580/93‑1 (MQ=255)       tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGACtcgct     >  1:340318/1‑93 (MQ=11)        ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgctt    <  2:3211946/93‑1 (MQ=16)        ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgctt    <  2:5115964/93‑1 (MQ=255)         cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttc   <  1:2507574/93‑1 (MQ=12)         cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttc   <  2:2975717/93‑1 (MQ=12)         cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttc   >  2:212819/1‑93 (MQ=12)         cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttc   <  1:734464/93‑1 (MQ=12)         cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttc   <  1:6631218/93‑1 (MQ=255)         cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttc   <  1:1986705/93‑1 (MQ=255)          ccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgatttg  >  1:5223738/1‑91 (MQ=11)                                                                                 |                      TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCG  >  NC_000913/1096536‑1096636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑