BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,209,664	(G)_5→6	coding (96/834 nt)	mcrA →	McrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,209,659	1	.	G	98.0%	470.8 / 1.1	151	coding (91/834 nt)	mcrA	McrA
Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (81/67); total (83/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

TTATGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGT  >  NC_000913/1209567‑1209744

ttatGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑t                                                                                         <  1:654943/93‑1 (MQ=255)

ttatGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑t                                                                                         >  2:4698800/1‑93 (MQ=255)

ttatGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑t                                                                                         <  2:1279695/93‑1 (MQ=255)

 tatGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑g                                                                                      >  2:6853220/1‑93 (MQ=255)

 tatGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑g                                                                                      >  2:6191473/1‑93 (MQ=255)

 tatGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑g                                                                                      >  2:53869/1‑93 (MQ=255)

  atGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑gg                                                                                     >  1:1086797/1‑93 (MQ=255)

  atGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑gg                                                                                     <  1:5478927/93‑1 (MQ=255)

   tGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑ggg                                                                                    <  2:7622279/93‑1 (MQ=255)

   tGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑ggg                                                                                    <  2:4749056/93‑1 (MQ=255)

   tGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑ggg                                                                                    <  2:7622277/93‑1 (MQ=255)

    gCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑gggg                                                                                   >  1:5992718/1‑93 (MQ=255)

    gCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑gggg                                                                                   >  2:5926955/1‑93 (MQ=255)

    gCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑gggg                                                                                   >  1:282943/1‑93 (MQ=255)

      aTGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑gggggg                                                                                 <  1:3392739/93‑2 (MQ=255)

      aTGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑gggggg                                                                                 >  2:614041/1‑92 (MQ=255)

       tGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑ggggggc                                                                                >  2:7362743/1‑91 (MQ=255)

       tGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑ggggggc                                                                                <  1:1025999/93‑3 (MQ=255)

       tGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑ggggggc                                                                                >  1:6932986/1‑91 (MQ=255)

       tGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑ggggggc                                                                                >  2:4580150/1‑91 (MQ=255)

             tGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTg                                                                           >  2:5717764/1‑93 (MQ=255)

              gATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGa                                                                          >  1:7736494/1‑93 (MQ=255)

              gATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGa                                                                          >  1:1389594/1‑93 (MQ=255)

               ataataATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGAc                                                                         >  1:4474476/1‑93 (MQ=255)

                taataaTGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGaga                                                                        >  1:7445145/1‑91 (MQ=255)

                 aataatGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAg                                                                       >  1:5858982/1‑93 (MQ=255)

                   taatGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGaa                                                                     >  2:6665633/1‑93 (MQ=255)

                    aatGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGaaa                                                                    >  1:347011/1‑93 (MQ=255)

                     atGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAAc                                                                   >  2:673310/1‑93 (MQ=255)

                      tGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACa                                                                  >  2:3833258/1‑93 (MQ=255)

                      tGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACa                                                                  <  2:6541754/93‑1 (MQ=255)

                         aaTTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAg                                                               <  2:5317569/93‑1 (MQ=255)

                         aaTTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAg                                                               <  1:2938024/93‑1 (MQ=255)

                           ttGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGAt                                                             <  1:6974549/93‑1 (MQ=255)

                           ttGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGAt                                                             >  2:2466250/1‑93 (MQ=255)

                              aaCTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTAc                                                          <  2:3644463/93‑1 (MQ=255)

                                cTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACaa                                                        <  2:681099/93‑1 (MQ=255)

                                cTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACaa                                                        <  1:4756993/93‑1 (MQ=255)

                                 tGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAat                                                       >  2:6438955/1‑93 (MQ=255)

                                 tGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAat                                                       >  2:32327/1‑93 (MQ=255)

                                 tGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAat                                                       >  1:2169650/1‑93 (MQ=255)

                                  gAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAata                                                      >  2:3346377/1‑93 (MQ=255)

                                  gAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAata                                                      >  1:3315597/1‑93 (MQ=255)

                                  gAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAata                                                      <  1:2731076/93‑1 (MQ=255)

                                    aaGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATc                                                    <  2:3191238/93‑1 (MQ=255)

                                    aaGCTGAGTGTTCGATAGGTGAAGAGGATGGGGTTTATGGTCTAATCCTTGAGTCGAG‑‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATc                                                    >  1:4122664/1‑93 (MQ=255)

                                     aGCTGTGTGTGCGATAGGTTAAGAGGATGGTGGTTATGGTCTAATCCCTGAGTCG‑TG‑GGGGGCCGGGTTTCAGAAACAAAGATTACAATATCg                                                   >  1:7021928/1‑93 (MQ=255)

                                     aGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCg                                                   <  1:2103273/93‑1 (MQ=255)

                                      gCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGc                                                  <  2:2822773/93‑1 (MQ=255)

                                       ttGAGTGTTCGATAGTTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGct                                                 <  2:2841821/92‑1 (MQ=255)

                                        tGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGctc                                                >  2:5405677/1‑93 (MQ=255)

                                        tGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGctc                                                <  1:1787096/93‑1 (MQ=255)

                                         gAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGctct                                               >  2:3438119/1‑93 (MQ=255)

                                          aGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCtt                                              <  1:238479/93‑1 (MQ=255)

                                          aGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCtt                                              >  2:1134725/1‑93 (MQ=255)

                                           gtgtTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGGGACAGAAACAAAGATTACAATATCGCTCTTg                                             >  1:445317/1‑93 (MQ=255)

                                             gtTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGAt                                           <  1:2319187/93‑1 (MQ=255)

                                             gtTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGAt                                           >  2:2495613/1‑93 (MQ=255)

                                              ttCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGAtt                                          >  2:7239409/1‑93 (MQ=255)

                                               tCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATta                                         <  1:5177872/93‑1 (MQ=255)

                                               tCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATta                                         <  1:6306128/93‑1 (MQ=255)

                                                 gATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATtata                                       <  2:924707/93‑1 (MQ=255)

                                                 gATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATtata                                       >  2:306643/1‑93 (MQ=255)

                                                 gATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGACGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATtata                                       <  1:5533671/93‑1 (MQ=255)

                                                   tAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TGGGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTatat                                      <  1:4912003/93‑1 (MQ=255)

                                                   tAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATc                                     >  1:7581899/1‑93 (MQ=255)

                                                   tAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATc                                     >  2:4525385/1‑93 (MQ=255)

                                                    aGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCa                                    <  1:6236505/93‑1 (MQ=255)

                                                      gTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCAtt                                  <  2:2623677/93‑1 (MQ=255)

                                                       tGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTg                                 >  2:1504332/1‑93 (MQ=255)

                                                        gAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGa                                >  2:2157932/1‑93 (MQ=255)

                                                         aaGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGaa                               >  2:6200233/1‑93 (MQ=255)

                                                          agagGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAAc                              >  2:4432294/1‑93 (MQ=255)

                                                          agagGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAAc                              >  2:3711684/1‑93 (MQ=255)

                                                          agagGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAAc                              >  2:2215664/1‑93 (MQ=255)

                                                           gagGATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACg                             >  1:7162450/1‑93 (MQ=255)

                                                             ggATGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACggt                           >  2:1865136/1‑93 (MQ=255)

                                                                tGGTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACggttgg                        >  2:5306585/1‑93 (MQ=255)

                                                                 ggTGTTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACggttggt                       <  2:4976670/93‑1 (MQ=255)

                                                                    gtTTATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGa                    <  2:2903287/93‑1 (MQ=255)

                                                                     tttATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGAt                   >  1:2713581/1‑93 (MQ=255)

                                                                     tttATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGAt                   >  1:1248301/1‑93 (MQ=255)

                                                                     tttATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGAt                   <  2:2364127/93‑1 (MQ=255)

                                                                      ttATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGAtt                  >  2:2220034/1‑93 (MQ=255)

                                                                       tATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTc                 >  1:1898705/1‑93 (MQ=255)

                                                                       tATGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTc                 <  2:6487438/93‑1 (MQ=255)

                                                                        aTGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCt                >  2:1065124/1‑93 (MQ=255)

                                                                         tGGTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTg               <  2:4610057/93‑1 (MQ=255)

                                                                          ggTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTgg              <  1:6704146/93‑1 (MQ=255)

                                                                          ggTCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTgg              >  2:6963617/1‑93 (MQ=255)

                                                                            tCTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGgtg            >  1:3321677/1‑93 (MQ=255)

                                                                             cTAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGgtgt           <  1:5055300/93‑1 (MQ=255)

                                                                              tAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTa          >  1:578048/1‑93 (MQ=255)

                                                                              tAATCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAAAGGATGGTTGATTCTGGTGTa          >  2:6961530/1‑93 (MQ=255)

                                                                               aaTCCTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTAt         >  2:2691102/1‑93 (MQ=255)

                                                                                   cTTGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCa     >  2:4134324/1‑93 (MQ=255)

                                                                                    ttGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCaa    <  2:2078581/93‑1 (MQ=255)

                                                                                    ttGAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCaa    >  2:1378445/1‑93 (MQ=255)

                                                                                    ttGAGTAG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCaa    <  1:5628087/93‑1 (MQ=255)

                                                                                      gAGTCG‑TG‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGt  <  2:7719115/93‑1 (MQ=255)

TTATGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCG‑T‑‑GGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGT  >  NC_000913/1209567‑1209744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`