BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	115,968	+G	coding (1132/1386 nt)	hofB ←	HofB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	115,964	1	.	G	98.5%	834.3 / 3.6	265	coding (1136/1386 nt)	hofB	HofB
Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (141/120); total (143/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

GACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑GG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCA  >  NC_000913/115872‑116051

gACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑t                                                                                            <  1:2761612/93‑1 (MQ=255)

gACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑t                                                                                            >  2:4773175/1‑93 (MQ=255)

 aCGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑g                                                                                          <  2:4416760/93‑1 (MQ=255)

   gAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑g                                                                                       <  2:1860733/93‑1 (MQ=255)

   gAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑g                                                                                       >  1:2047272/1‑93 (MQ=255)

   gAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑g                                                                                       >  2:2024418/1‑93 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑gg                                                                                      >  1:2885833/1‑93 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑gg                                                                                      >  2:2652182/1‑93 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCACAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAT‑T‑gg‑gg                                                                                      >  2:5596287/1‑93 (MQ=255)

       gACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑gggca                                                                                   <  1:141564/93‑4 (MQ=255)

       gACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑gggca                                                                                   >  1:4390002/1‑90 (MQ=255)

       gACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑gg‑gggca                                                                                   <  2:3141041/93‑4 (MQ=255)

          cGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAgcg                                                                                >  1:5941239/1‑93 (MQ=255)

          cGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAgcg                                                                                <  1:6229611/93‑1 (MQ=255)

           ggCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAgcgg                                                                               <  1:1779513/93‑1 (MQ=255)

            gCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAgcggc                                                                              >  1:3770590/1‑93 (MQ=255)

             cGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAgcggcg                                                                             <  1:2869736/93‑1 (MQ=255)

              gTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGa                                                                            >  1:5864432/1‑93 (MQ=255)

               ttATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGAt                                                                           <  2:6142991/93‑1 (MQ=255)

               ttATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGAt                                                                           <  2:6862262/93‑1 (MQ=255)

                tATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATg                                                                          >  1:5540179/1‑93 (MQ=255)

                 aTGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAACCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATgg                                                                         <  1:4876324/93‑1 (MQ=255)

                  tGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGc                                                                        >  1:2894398/1‑93 (MQ=255)

                   gggCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGcc                                                                       >  2:2091428/1‑93 (MQ=255)

                   gggCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGcc                                                                       >  2:6227208/1‑93 (MQ=255)

                   gggCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGcc                                                                       >  1:7276203/1‑93 (MQ=255)

                    ggCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCa                                                                      >  1:5531591/1‑93 (MQ=255)

                     gCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCAt                                                                     >  2:7207188/1‑93 (MQ=255)

                       aGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATAc                                                                   >  1:407865/1‑93 (MQ=255)

                       aGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATAc                                                                   >  2:3724198/1‑93 (MQ=255)

                        gAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACACCCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACa                                                                  <  2:839933/93‑1 (MQ=255)

                        gAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACa                                                                  <  1:2627640/93‑1 (MQ=255)

                         aaCTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACAt                                                                 <  1:1211483/93‑1 (MQ=255)

                          aCTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑GG‑GGACAGCGGCGATGGCCATACAtt                                                                <  2:7782953/93‑1 (MQ=255)

                            ttCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGt                                                              >  2:7835523/1‑93 (MQ=255)

                            ttCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGt                                                              >  2:1678816/1‑93 (MQ=255)

                            ttCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGt                                                              <  2:3403999/93‑1 (MQ=255)

                            ttCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGt                                                              <  2:4220853/93‑1 (MQ=255)

                               aaaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTg                                                           <  1:6544269/93‑1 (MQ=255)

                               aaaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTg                                                           >  2:161348/1‑93 (MQ=255)

                               aaaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTg                                                           <  2:3858182/93‑1 (MQ=255)

                                aaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTgg                                                          >  2:7694876/1‑93 (MQ=255)

                                aaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTgg                                                          <  1:1551043/93‑1 (MQ=255)

                                 aTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGa                                                         >  1:3330987/1‑93 (MQ=255)

                                  tAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGaa                                                        >  1:3581270/1‑93 (MQ=255)

                                  tAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGaa                                                        <  2:465554/93‑1 (MQ=255)

                                   aaGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATCCATTGTCTGGagg                                                       >  1:1910486/1‑91 (MQ=255)

                                    aGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAAtg                                                      <  2:6064662/93‑1 (MQ=255)

                                     ggCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAAtgg                                                     >  2:5743890/1‑92 (MQ=255)

                                      gCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAAtgtg                                                    <  1:6117060/93‑1 (MQ=255)

                                        cGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGa                                                  <  1:7160248/93‑1 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 <  1:6805882/93‑1 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 >  2:2263976/1‑93 (MQ=255)

                                          tACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATg                                                >  2:5971826/1‑93 (MQ=255)

                                             gACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGc                                             <  2:6465183/93‑1 (MQ=255)

                                              aCCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCt                                            >  2:5837294/1‑93 (MQ=255)

                                              aCCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCt                                            >  2:6169449/1‑93 (MQ=255)

                                              aCCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCt                                            >  1:5235825/1‑93 (MQ=255)

                                               ccATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTc                                           >  1:1106144/1‑93 (MQ=255)

                                               ccATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTc                                           <  2:6323001/93‑1 (MQ=255)

                                                cATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 >  1:4574862/1‑86 (MQ=255)

                                                 aTAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTccc                                         >  2:2755344/1‑93 (MQ=255)

                                                 aTAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTccc                                         <  2:4624390/93‑1 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                        >  2:2442558/1‑93 (MQ=255)

                                                     aaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtg                                     <  2:1795885/93‑1 (MQ=255)

                                                      aaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgc                                    <  1:4538087/93‑1 (MQ=255)

                                                      aaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgc                                    <  1:4798262/93‑1 (MQ=255)

                                                      aaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgc                                    <  2:1238889/93‑1 (MQ=255)

                                                       aCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgct                                   <  2:1388723/93‑1 (MQ=255)

                                                       aCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgct                                   >  2:7435782/1‑93 (MQ=255)

                                                       aCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgct                                   >  2:1512054/1‑93 (MQ=255)

                                                         cGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgctgc                                 <  1:1806200/93‑1 (MQ=255)

                                                         cGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgctgc                                 <  1:4236241/93‑1 (MQ=255)

                                                          gTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGcc                                >  1:5155218/1‑93 (MQ=255)

                                                          gTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGcc                                <  2:3498277/93‑1 (MQ=255)

                                                          gTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGcc                                >  1:7610383/1‑93 (MQ=255)

                                                            ggTAGCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCgg                              <  1:5672711/93‑1 (MQ=255)

                                                              tAGCAATGTACACAACCGGGTGCCTGCCAGCG‑GG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATATGGATGGGCTCCCCTTGCTGCCGGCg                            >  1:937007/1‑93 (MQ=255)

                                                                gCAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGAc                          >  1:637912/1‑93 (MQ=255)

                                                                gCAATGTACACAACCGGGTGCCTGCCAG‑T‑GG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACa                         >  1:2101184/1‑93 (MQ=255)

                                                                 cAATGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACa                         >  2:2864953/1‑93 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        <  1:6479768/93‑1 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        <  1:6776889/93‑1 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        <  2:4713534/93‑1 (MQ=255)

                                                                   aTGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAt                       >  1:5079092/1‑93 (MQ=255)

                                                                    tGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtg                      <  2:5086588/93‑1 (MQ=255)

                                                                    tGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtg                      >  2:7594768/1‑93 (MQ=255)

                                                                    tGTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtg                      >  2:6568005/1‑93 (MQ=255)

                                                                     gTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtgt                     <  1:7433404/93‑1 (MQ=255)

                                                                     gTACACAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtgt                     <  1:3054408/93‑1 (MQ=255)

                                                                      tACACAGCCGGGTGCCTGCCAG‑T‑GGAGT‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtgtg                    <  1:6960108/93‑1 (MQ=255)

                                                                       acacAACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTgg                   <  1:5420775/93‑1 (MQ=255)

                                                                        cacaACCGGGTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTggg                  >  1:1680373/1‑93 (MQ=255)

                                                                         acaACCGGGTGCCTGCGAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGc                 >  2:5218746/1‑93 (MQ=255)

                                                                               gggTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGtt           >  2:5017819/1‑93 (MQ=255)

                                                                                ggTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGttt          <  1:2262562/93‑1 (MQ=255)

                                                                                 gTGCCTGCCAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTg         <  1:2531701/93‑1 (MQ=255)

                                                                                    cctgccAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGt      >  2:1011701/1‑93 (MQ=255)

                                                                                     ctgccAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTa     >  1:7913916/1‑93 (MQ=255)

                                                                                       gccAG‑TGGG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCTGGCGACAATGTGGGCAAAGTTTGCGTAcc   <  2:6726709/93‑1 (MQ=255)

GACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAG‑T‑GG‑GG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCA  >  NC_000913/115872‑116051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`