BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,033	(C)_5→6	coding (1149/7152 nt)	b1978 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,028	1	.	C	93.2%	519.5 / 30.1	176	coding (1144/7152 nt)	b1978	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (4/8); new base C (106/58); total (110/66)
Fisher's exact test for biased strand distribution p-value = 5.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 212 total aligned reads displayed.

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT  >  NC_000913/2043936‑2044115

tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACtat‑a                                                                                            >  2:1331614/1‑93 (MQ=255)

tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACtat‑a                                                                                            >  2:7870205/1‑93 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c                                                                                          <  2:1943135/93‑1 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c                                                                                          <  2:3767986/93‑1 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c                                                                                          <  1:2945117/93‑1 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c                                                                                          >  1:2971691/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑c                                                                                        >  1:1568702/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑c                                                                                        >  2:7139004/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑c                                                                                        <  1:7072797/93‑1 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑c                                                                                     >  2:5895758/1‑93 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑c                                                                                     >  2:7006129/1‑93 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑c                                                                                     >  1:7334194/1‑93 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑c                                                                                     >  2:4272559/1‑93 (MQ=255)

     gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑cc                                                                                    >  2:4091847/1‑93 (MQ=255)

       aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑ccct                                                                                  >  2:467867/1‑91 (MQ=255)

       aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑ccct                                                                                  >  1:5200933/1‑91 (MQ=255)

         aGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑cccttc                                                                                <  2:3406473/93‑5 (MQ=255)

         aGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑c‑cc‑cccttc                                                                                <  1:1062838/93‑5 (MQ=255)

           tattatGGCGATGAAGTTGCCCTGTTCGATAAAGACGATCGGCAAAGCAATCCTCATGCCATAACCGCCGGACTTAAATAT‑A‑C‑CCACCTTccc                                                                               >  2:2464175/1‑93 (MQ=255)

            attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCg                                                                              <  2:7922147/93‑1 (MQ=255)

             ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGc                                                                             >  2:6234896/1‑93 (MQ=255)

              tatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCt                                                                            <  1:6972486/93‑1 (MQ=255)

              tatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCt                                                                            <  1:7791856/93‑1 (MQ=255)

               atGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCtg                                                                           >  2:6904584/1‑93 (MQ=255)

                tGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCtga                                                                          >  1:2215535/1‑93 (MQ=255)

                    gATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGAc                                                                      >  1:6500656/1‑93 (MQ=255)

                     aTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATCC‑C‑CC‑CCTTCCCGCTGATGAcc                                                                     <  2:141841/93‑1 (MQ=255)

                         aGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCa                                                                 >  2:7307562/1‑93 (MQ=255)

                            ggCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAgcg                                                              <  1:39198/93‑1 (MQ=255)

                             gCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAgcgc                                                             <  1:4573109/93‑1 (MQ=255)

                               ccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCgg                                                           >  1:187833/1‑93 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGa                                                          <  2:2806609/93‑1 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGa                                                          >  1:6607194/1‑93 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGa                                                          >  1:5836723/1‑93 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGa                                                          >  2:4992776/1‑93 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAg                                                         >  1:1621718/1‑93 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAg                                                         >  1:6316376/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  2:3557882/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  2:6692699/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  2:493544/1‑93 (MQ=255)

                                     cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAAc                                                     <  2:6570148/93‑1 (MQ=255)

                                     cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAAc                                                     >  2:4811409/1‑93 (MQ=255)

                                      gATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACg                                                    >  1:4284406/1‑93 (MQ=255)

                                      gATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACg                                                    >  1:7467200/1‑93 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATCC‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                   <  2:4018661/93‑1 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                   >  1:3401772/1‑93 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                   >  1:962045/1‑93 (MQ=255)

                                            gACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAggg                                              >  2:1372249/1‑93 (MQ=255)

                                            gACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAggg                                              >  1:6044053/1‑93 (MQ=255)

                                             aCGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAggga                                             >  1:6080055/1‑92 (MQ=255)

                                              cGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTa                                            <  1:5388123/93‑1 (MQ=255)

                                                aTCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTaaa                                          <  2:5745143/93‑1 (MQ=255)

                                                  cgcgaaaGTAATCCTCATGCCATAACCGCTGGACTTAACTATGC‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACa                                        <  2:299877/89‑1 (MQ=255)

                                                  cGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACa                                        <  2:5600766/93‑1 (MQ=255)

                                                  cGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCACGGTAAACa                                        >  1:4010256/1‑93 (MQ=255)

                                                   ggCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAg                                       >  2:3980051/1‑93 (MQ=255)

                                                   ggCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTGCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAg                                       <  1:7608908/93‑1 (MQ=255)

                                                    gCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAgg                                      <  2:3923613/93‑1 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    >  1:5242290/1‑93 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    >  2:2412798/1‑93 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    <  2:7516893/93‑1 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    >  1:3710901/1‑93 (MQ=255)

                                                        aGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGa                                  >  2:1863710/1‑93 (MQ=255)

                                                         gTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaa                                 >  2:581832/1‑93 (MQ=255)

                                                          tAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaa                                <  1:1111387/93‑1 (MQ=255)

                                                          tAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaa                                >  2:60200/1‑93 (MQ=255)

                                                          tAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaa                                >  1:2496134/1‑93 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               <  1:2100207/93‑1 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               >  1:7477029/1‑93 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               <  1:7422832/93‑1 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               <  1:5499190/93‑1 (MQ=255)

                                                            aTCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAt                              <  2:6422993/93‑1 (MQ=255)

                                                             tCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATg                             >  1:7358677/1‑93 (MQ=255)

                                                             tCCTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATg                             >  1:2876582/1‑93 (MQ=255)

                                                               cTCATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGac                           >  1:4216427/1‑93 (MQ=255)

                                                                 cATGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGacac                         <  1:3308310/93‑1 (MQ=255)

                                                                  aTGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAcc                        >  1:3776853/1‑93 (MQ=255)

                                                                   tGCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAccc                       >  2:2200889/1‑93 (MQ=255)

                                                                    gCCATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCg                      >  1:6522561/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    >  2:1266047/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    >  2:6477660/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    >  1:1973957/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTAT‑A‑CACC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    >  2:7586049/1‑93 (MQ=255)

                                                                       aTAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGttt                   >  1:6422548/1‑93 (MQ=255)

                                                                        tAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                  <  1:1659724/93‑1 (MQ=255)

                                                                        tAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                  >  2:1655454/1‑93 (MQ=255)

                                                                        tAACCGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                  <  2:5660707/93‑1 (MQ=255)

                                                                            cGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCg              <  1:4420668/93‑1 (MQ=255)

                                                                            cGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCg              <  1:5642403/93‑1 (MQ=255)

                                                                            cGCTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCg              <  1:7680506/93‑1 (MQ=255)

                                                                             gCTGGACTTAACTAT‑A‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTc            >  2:2007584/1‑93 (MQ=255)

                                                                              cTGGACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTc            >  1:6726908/1‑93 (MQ=255)

                                                                               tGGACTTAACTATGC‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCg           <  2:6709646/93‑1 (MQ=255)

                                                                                ggACTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGa          >  1:5103280/1‑93 (MQ=255)

                                                                                  aCTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAtt        <  1:3677365/93‑1 (MQ=255)

                                                                                   cTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAttt       >  1:7283441/1‑93 (MQ=255)

                                                                                   cTTAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAttt       >  1:1356631/1‑93 (MQ=255)

                                                                                    ttAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAtttt      >  1:2276160/1‑93 (MQ=255)

                                                                                     tAACTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTa     >  1:4118182/1‑93 (MQ=255)

                                                                                       aCTAT‑ACC‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTacc   >  2:6821286/1‑93 (MQ=255)

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑C‑CC‑CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT  >  NC_000913/2043936‑2044115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`