Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,376,227 +G coding (1053/1671 nt) menD ← 2‑oxoglutarate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,376,2251.G99.1% 660.3 / ‑1.7 215coding (1055/1671 nt)menD2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (117/96);  total (118/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 243 total aligned reads displayed.

GTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑GGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTC  >  NC_000913/2376133‑2376312
                                                                                             |                                                                                       
gTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCa                                                                                          <  2:2403282/93‑1 (MQ=255)
gTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCa                                                                                          <  1:3994212/93‑1 (MQ=255)
gTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCa                                                                                          >  1:4315515/1‑93 (MQ=255)
 ttCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑g                                                                                        <  1:7417725/93‑1 (MQ=255)
  tCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑gg                                                                                       >  1:1195492/1‑93 (MQ=255)
   cAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑ggg                                                                                      <  1:651870/93‑2 (MQ=255)
    aGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑gggc                                                                                     <  1:3660189/93‑3 (MQ=255)
      gCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑gggcgc                                                                                   <  1:5161169/93‑5 (MQ=255)
         gATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCggg                                                                                 >  2:3623657/1‑93 (MQ=255)
         gATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCggg                                                                                 >  1:4732235/1‑93 (MQ=255)
            aGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGAtt                                                                              <  2:7570342/93‑1 (MQ=255)
            aGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGAtt                                                                              >  1:2388197/1‑93 (MQ=255)
             gTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGAttt                                                                             >  2:4092224/1‑93 (MQ=255)
             gTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGAttt                                                                             >  2:6271604/1‑93 (MQ=255)
               cgaaGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCa                                                                           <  2:4810139/90‑1 (MQ=255)
                gCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCaa                                                                          >  2:1953383/1‑93 (MQ=255)
                  aGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACg                                                                        >  1:6495954/1‑93 (MQ=255)
                   gatgcgatgcGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGc                                                                       >  1:2382979/1‑93 (MQ=255)
                     tgcgatgcgCCAGTTGCCCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCAc                                                                     <  2:2143716/93‑1 (MQ=255)
                      gcgatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCAcc                                                                    <  2:1417449/93‑1 (MQ=255)
                      gcgatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCAcc                                                                    <  1:263608/93‑1 (MQ=255)
                      gcgatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCAcc                                                                    <  2:2807937/93‑1 (MQ=255)
                        gatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAg                                                                  <  1:5793866/93‑1 (MQ=255)
                        gatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAg                                                                  <  2:2533806/93‑1 (MQ=255)
                        gatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAg                                                                  <  2:3199500/93‑1 (MQ=255)
                        gatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAg                                                                  <  1:1462231/93‑1 (MQ=255)
                         atgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAgg                                                                 >  1:99578/1‑93 (MQ=255)
                         atgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAgg                                                                 <  1:5649641/93‑1 (MQ=255)
                         atgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAgg                                                                 >  2:7643004/1‑93 (MQ=255)
                         atgcgCCAGTTGCGCTGCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAgg                                                                 <  2:2277714/93‑1 (MQ=255)
                            cgcCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCt                                                              <  1:254948/93‑1 (MQ=255)
                             gcCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTg                                                             <  1:1749422/93‑1 (MQ=255)
                              ccAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTgg                                                            >  2:5209592/1‑93 (MQ=255)
                               cAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTggg                                                           >  1:3006353/1‑92 (MQ=255)
                               cAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGc                                                           <  1:6406530/93‑1 (MQ=255)
                                 gTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGt                                                         >  1:1018679/1‑93 (MQ=255)
                                  ttGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGtt                                                        >  1:1523053/1‑93 (MQ=255)
                                   tGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGttt                                                       >  1:5072878/1‑93 (MQ=255)
                                    gcgcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGtttt                                                      <  1:289422/93‑1 (MQ=255)
                                     cgcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGttttt                                                     >  1:955537/1‑93 (MQ=255)
                                      gcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTc                                                    <  1:5425281/93‑1 (MQ=255)
                                      gcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTc                                                    >  2:3656938/1‑93 (MQ=255)
                                      gcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTc                                                    <  2:1320250/93‑1 (MQ=255)
                                         tCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGc                                                 <  2:6833340/93‑1 (MQ=255)
                                         tCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGc                                                 >  2:4582203/1‑93 (MQ=255)
                                            ccAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGCTTTCAACGCACCAGGGCTGGCGTTTTTCTGCCgg                                              >  1:5515867/1‑93 (MQ=255)
                                            ccAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCgg                                              >  1:6091939/1‑93 (MQ=255)
                                             cAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGa                                             >  2:5551316/1‑93 (MQ=255)
                                             cAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGa                                             >  2:5305509/1‑93 (MQ=255)
                                               aaCGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATg                                           >  1:6713219/1‑93 (MQ=255)
                                                aCGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGc                                          >  1:1991382/1‑93 (MQ=255)
                                                 cGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCa                                         <  1:2926210/93‑1 (MQ=255)
                                                  gCATCACGGCGGGCAATAACAGCCTGCATTGCCTGTTCAGCCAGGGCGCGGGATTTCAACGCAACAGGGATGGCGTTTTTCTGCCGGATGCAg                                        <  1:4979934/93‑1 (MQ=255)
                                                   cATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGc                                       <  1:6161898/93‑1 (MQ=255)
                                                   cATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGc                                       <  1:2664707/93‑1 (MQ=255)
                                                    aTCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCt                                      >  1:2288754/1‑93 (MQ=255)
                                                     tCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTc                                     >  2:6032369/1‑93 (MQ=255)
                                                       aCGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTcca                                   <  1:7485091/93‑1 (MQ=255)
                                                        cggcggGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccag                                  >  2:270700/1‑93 (MQ=255)
                                                        cggcggGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGACTGGCGTTTTTCTGCCGGATGCAGCTccag                                  >  2:3914561/1‑93 (MQ=255)
                                                         ggcggGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagc                                 <  2:7103393/93‑1 (MQ=255)
                                                         ggcggGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagc                                 >  2:347426/1‑93 (MQ=255)
                                                           ggggCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagcca                               <  2:1856638/92‑1 (MQ=255)
                                                           cggGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagcca                               <  1:5399062/93‑1 (MQ=255)
                                                           cggGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagcca                               <  2:6508636/93‑1 (MQ=255)
                                                           cggGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagcca                               <  2:1472085/93‑1 (MQ=255)
                                                            gggCAATACCCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagccag                              <  1:2527604/93‑1 (MQ=255)
                                                              gCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTc                            <  2:6935526/93‑1 (MQ=255)
                                                              gCAATAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTc                            >  1:5317693/1‑93 (MQ=255)
                                                                aaTAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCgg                          >  2:7449257/1‑93 (MQ=255)
                                                                aaTAACCGCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCgg                          >  2:3296947/1‑93 (MQ=255)
                                                                       gCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATAtt                   <  2:7276373/93‑1 (MQ=255)
                                                                       gCCTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATAtt                   <  2:1745901/93‑1 (MQ=255)
                                                                        ccTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTg                  >  2:7754692/1‑93 (MQ=255)
                                                                        ccTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTg                  <  2:6795997/93‑1 (MQ=255)
                                                                         cTGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTgg                 >  1:434844/1‑93 (MQ=255)
                                                                          tGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGc                >  1:4756518/1‑93 (MQ=255)
                                                                          tGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGc                >  2:6233763/1‑93 (MQ=255)
                                                                          tGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGc                >  1:3485595/1‑93 (MQ=255)
                                                                          tGCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGc                <  2:3079327/93‑1 (MQ=255)
                                                                           gCATTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCa               >  2:4951449/1‑93 (MQ=255)
                                                                             aTTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAAt             >  2:2675885/1‑93 (MQ=255)
                                                                             aTTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAAt             >  1:6790376/1‑93 (MQ=255)
                                                                             aTTGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAAt             <  1:420029/93‑1 (MQ=255)
                                                                             aTTCCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAAt             <  2:4674037/93‑1 (MQ=255)
                                                                              ttGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAAtt            <  1:1240091/93‑1 (MQ=255)
                                                                              ttGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAAtt            >  1:2907939/1‑93 (MQ=255)
                                                                               tGCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTa           <  2:5835378/93‑1 (MQ=255)
                                                                                gCCTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTaa          >  2:5601961/1‑93 (MQ=255)
                                                                                 ccTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAg         <  1:7758102/93‑1 (MQ=255)
                                                                                  cTGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGc        <  2:4443772/93‑1 (MQ=255)
                                                                                   tGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCg       >  2:2111809/1‑93 (MQ=255)
                                                                                   tGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCg       >  1:3092680/1‑93 (MQ=255)
                                                                                   tGTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCg       >  1:4571243/1‑93 (MQ=255)
                                                                                    gTTCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGa      <  1:4532977/93‑1 (MQ=255)
                                                                                     ttCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGAc     <  1:3276219/93‑1 (MQ=255)
                                                                                     ttCCGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGAc     <  1:5819850/93‑1 (MQ=255)
                                                                                      tCCGCCAGGGCGCGGGATTTCAACGCTCCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACg    <  2:1270035/93‑1 (MQ=255)
                                                                                       ccGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGt   >  2:6400602/1‑93 (MQ=255)
                                                                                       ccGCCAGGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGt   <  2:5936907/93‑1 (MQ=255)
                                                                                             |                                                                                       
GTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑GGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTC  >  NC_000913/2376133‑2376312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑