BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,458,650	+G	intergenic (‑161/+20)	b2342 ← / ← b2343	putative acyltransferase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,458,646	1	.	G	98.4%	578.6 / 1.2	186	intergenic (‑157/+24)	b2342/b2343	putative acyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (111/72); total (113/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 209 total aligned reads displayed.

ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA  >  NC_000913/2458554‑2458734

aCATTTAGCAAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACa                                                                                             <  1:5779965/93‑1 (MQ=255)

 cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g                                                                                           >  2:750198/1‑93 (MQ=255)

 cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g                                                                                           >  2:2874033/1‑93 (MQ=255)

  aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑g                                                                                         >  1:939074/1‑93 (MQ=255)

  aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑g                                                                                         >  2:3779993/1‑93 (MQ=255)

  aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑g                                                                                         >  1:1685575/1‑93 (MQ=255)

  aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑g                                                                                         <  2:3531772/93‑1 (MQ=255)

   tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑g‑g                                                                                       <  1:4965555/93‑1 (MQ=255)

    ttAGCCAAGTGGAGAAAAGGGAAAGTGGGATCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑g‑gg                                                                                      >  1:6739990/1‑93 (MQ=255)

          aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGcgtac                                                                                 >  2:7739692/1‑93 (MQ=255)

          aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGcgtac                                                                                 >  1:6590722/1‑93 (MQ=255)

          aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGcgtac                                                                                 >  1:33417/1‑93 (MQ=255)

           aGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGcgtacg                                                                                >  1:4911657/1‑93 (MQ=255)

            gTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGcgtacgt                                                                               >  1:7885791/1‑93 (MQ=255)

            gTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGcgtacgt                                                                               >  2:4578569/1‑93 (MQ=255)

            gTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGcgtacgt                                                                               >  1:6744066/1‑93 (MQ=255)

             tGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGcgtacgta                                                                              >  2:7640561/1‑93 (MQ=255)

                   aaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCgag                                                                        >  1:6137656/1‑93 (MQ=255)

                   aaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGACCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCgag                                                                        >  1:680933/1‑93 (MQ=255)

                    aaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCgaga                                                                       >  1:4423380/1‑93 (MQ=255)

                    aaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCgaga                                                                       >  2:7120072/1‑93 (MQ=255)

                        gAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGaa                                                                   <  1:249153/93‑1 (MQ=255)

                         aaaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAAt                                                                  <  1:6681601/93‑1 (MQ=255)

                          aaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAAta                                                                 >  1:7078562/1‑92 (MQ=255)

                           aGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTa                                                                <  2:6868876/93‑1 (MQ=255)

                            atGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTaa                                                               <  1:1939897/92‑1 (MQ=255)

                             tGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAAc                                                              <  1:1235113/93‑1 (MQ=255)

                               gtaGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGc                                                            >  2:6963923/3‑93 (MQ=255)

                                gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCa                                                           >  2:5637336/1‑93 (MQ=255)

                                gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCa                                                           <  1:5468964/93‑1 (MQ=255)

                                gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCa                                                           >  1:6335492/1‑93 (MQ=255)

                                  gCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGa                                                         <  1:7853374/93‑1 (MQ=255)

                                  gCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGa                                                         >  1:1325170/1‑93 (MQ=255)

                                   cTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGAc                                                        >  1:389687/1‑93 (MQ=255)

                                    tATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                       >  1:5929054/1‑93 (MQ=255)

                                    tATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                       <  2:6811429/93‑1 (MQ=255)

                                      tGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                                     <  2:4031457/93‑1 (MQ=255)

                                         cacaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                  >  1:1139452/1‑93 (MQ=255)

                                         cacaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                  >  1:1804571/1‑93 (MQ=255)

                                          acaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                 <  2:6425560/93‑1 (MQ=255)

                                          acaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                 >  2:1605459/1‑93 (MQ=255)

                                          acaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGGACGTACCCGAGAGGAATTAACGCAGACCCAggcg                                                 >  2:5224557/1‑90 (MQ=255)

                                          acaGAGAAAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                 <  1:3033563/93‑1 (MQ=255)

                                           caGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTgg                                                >  2:1171831/1‑93 (MQ=255)

                                            agagagAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                               >  1:4795285/1‑93 (MQ=255)

                                            agagagAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                               >  2:7815128/1‑93 (MQ=255)

                                            agagagAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                               >  1:816371/1‑93 (MQ=255)

                                             gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                              <  2:4805853/93‑1 (MQ=255)

                                             gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                              >  1:231908/1‑93 (MQ=255)

                                                agaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                           <  2:6308394/93‑1 (MQ=255)

                                                 gaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                          >  2:7163570/1‑93 (MQ=255)

                                                  aaaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                         <  2:2761832/93‑1 (MQ=255)

                                                  aaaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                         >  2:2302675/1‑93 (MQ=255)

                                                   aaaaaaaaAGAGAAAGGAAGACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACCCAGACCCAGCTGGAAAATCAg                                        <  2:5707888/93‑1 (MQ=255)

                                                   aaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                                        >  1:7064733/1‑93 (MQ=255)

                                                   aaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                                        >  2:2374080/1‑93 (MQ=255)

                                                   aaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                                        <  2:616032/93‑1 (MQ=255)

                                                   aaaGAAGAAGAGAAAAAAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                                        <  2:185866/93‑1 (MQ=255)

                                                     agaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GTG‑GGCGGACGTACTCGAGAGGAAATTACGCAGACCCAGCTGGAAAATTAgta                                      >  2:7255256/1‑91 (MQ=255)

                                                     agaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAt                                     >  2:6855416/1‑93 (MQ=255)

                                                      gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAt                                     >  2:2470231/1‑93 (MQ=255)

                                                      gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAt                                     <  2:5482916/93‑1 (MQ=255)

                                                       aagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAtt                                    <  1:7538073/93‑1 (MQ=255)

                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAttt                                   >  2:165546/1‑93 (MQ=255)

                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAttt                                   >  1:2409438/1‑93 (MQ=255)

                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAttt                                   <  2:4900363/93‑1 (MQ=255)

                                                         gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                  <  1:779971/93‑1 (MQ=255)

                                                         gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                  >  2:7436076/1‑93 (MQ=255)

                                                         aaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                  <  1:2943566/92‑1 (MQ=255)

                                                          aagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                 <  2:444191/93‑1 (MQ=255)

                                                           agagAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGAATTTCAg                                >  2:6239166/1‑93 (MQ=255)

                                                           agagAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATAAGCATTTCac                                >  1:1780568/1‑92 (MQ=255)

                                                            gaaaaaaaaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                               <  1:5756648/90‑1 (MQ=255)

                                                               aaaagaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                            >  1:1751880/1‑93 (MQ=255)

                                                               aaaagaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                            <  1:7159270/93‑1 (MQ=255)

                                                               aaaaaaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                            <  1:6985746/93‑1 (MQ=255)

                                                                aaagaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                           >  2:598635/1‑93 (MQ=255)

                                                                 aagaaaaCGCCCCTGCCCGCAACTGACC‑G‑GCGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                          <  1:1631120/93‑1 (MQ=255)

                                                                 aagaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                          >  2:6681549/1‑93 (MQ=255)

                                                                 aagaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                          >  1:3562370/1‑93 (MQ=255)

                                                                  agaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                         <  2:1382712/93‑1 (MQ=255)

                                                                  agaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                         >  1:2647468/1‑93 (MQ=255)

                                                                  agaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                         >  2:588844/1‑93 (MQ=255)

                                                                   gaaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                        <  1:4628256/93‑1 (MQ=255)

                                                                    aaaaCGCCCCTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGCGGAATTAACGCAGACCCAGCTGGAAAATCAGCAGTTCAGCTTCGCAgg                       >  1:6975124/1‑93 (MQ=255)

                                                                          ccccTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                 <  2:132210/93‑1 (MQ=255)

                                                                           cccTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt                <  1:4098201/93‑1 (MQ=255)

                                                                           cccTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt                <  1:5246223/93‑1 (MQ=255)

                                                                             cTGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa              <  2:3883791/93‑1 (MQ=255)

                                                                              tGCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaa             <  2:2858952/93‑1 (MQ=255)

                                                                               gCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa            <  2:2199036/93‑1 (MQ=255)

                                                                               gCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa            <  1:374238/93‑1 (MQ=255)

                                                                               gCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa            >  2:1676008/1‑93 (MQ=255)

                                                                               gCCAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa            <  2:3050150/93‑1 (MQ=255)

                                                                                ccAGCAACTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt           >  2:1976225/1‑93 (MQ=255)

                                                                                     aaCTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGata      <  2:4225764/93‑1 (MQ=255)

                                                                                     aaCTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGata      >  1:7363303/1‑93 (MQ=255)

                                                                                     aaCTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGAAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGata      >  2:5810201/1‑93 (MQ=255)

                                                                                      aCTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat     <  1:4607709/93‑1 (MQ=255)

                                                                                       cTGACAGG‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGAGAAATCGATATc    >  2:5221444/1‑93 (MQ=255)

ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑G‑GGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA  >  NC_000913/2458554‑2458734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`