BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,563,585	(C)_5→6	coding (1320/1404 nt)	b2451 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,563,580	1	.	C	96.5%	685.9 / 15.7	227	coding (1325/1404 nt)	b2451	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (4/4); new base C (104/115); total (108/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 265 total aligned reads displayed.

GCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑T‑‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAA  >  NC_000913/2563488‑2563667

gCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑t                                                                                            <  1:7763885/93‑1 (MQ=255)

   aaTACCGCATTCAGGAAGGAAATGCGAGGGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGAGAACCAATGACGATATA‑G‑T‑‑ctc                                                                                       >  1:1068026/1‑91 (MQ=255)

    aTACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCACAAAGAGGCGTACCAATGTCGATATA‑G‑T‑‑c‑ccc                                                                                     >  1:4974256/1‑93 (MQ=255)

      aCCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑T‑‑c‑ccccc                                                                                   <  1:512464/93‑2 (MQ=255)

      aCCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑T‑‑c‑ccccc                                                                                   >  2:1574831/1‑92 (MQ=255)

       ccGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑T‑‑c‑cccccg                                                                                  <  1:2816477/93‑3 (MQ=255)

           aTTCAGGAAGGAAATGCGAGTGATCTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCcgcgcg                                                                               >  2:7586599/1‑93 (MQ=255)

            ttCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCgg                                                                              >  2:1308797/1‑93 (MQ=255)

             tCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGa                                                                             >  2:4552583/1‑93 (MQ=255)

                ggaaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACaa                                                                          <  1:620282/93‑1 (MQ=255)

                 gaaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGG‑C‑‑C‑CCCCGCGCGGACAAt                                                                         >  2:4212742/1‑93 (MQ=255)

                 gaaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAAt                                                                         <  1:434182/93‑1 (MQ=255)

                    ggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAATAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCAc                                                                      >  2:7005705/1‑93 (MQ=255)

                    ggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCAc                                                                      <  1:1947144/93‑1 (MQ=255)

                    ggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCAc                                                                      >  2:4931445/1‑93 (MQ=255)

                     gaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACt                                                                     >  2:3289463/1‑93 (MQ=255)

                      aaaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACtt                                                                    <  2:3802430/93‑1 (MQ=255)

                      aaaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACtt                                                                    <  2:2287329/93‑1 (MQ=255)

                       aaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTc                                                                   >  1:5627928/1‑93 (MQ=255)

                       aaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTc                                                                   <  1:1798277/93‑1 (MQ=255)

                       aaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTc                                                                   >  2:4072284/1‑93 (MQ=255)

                          gCGAGTGATTTCACCTTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTc                                                                <  2:104461/93‑1 (MQ=255)

                            gAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCaa                                                              <  1:2099856/93‑1 (MQ=255)

                             aGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAAt                                                             <  2:1943296/93‑1 (MQ=255)

                              gTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATg                                                            <  2:5130228/93‑1 (MQ=255)

                               tGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGa                                                           <  1:3237274/93‑1 (MQ=255)

                               tGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGa                                                           >  2:3468003/1‑93 (MQ=255)

                                gATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGAc                                                          <  1:522098/93‑1 (MQ=255)

                                 aTTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACt                                                         <  1:7679705/93‑1 (MQ=255)

                                   ttCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGc                                                       >  2:6047830/1‑93 (MQ=255)

                                    tcaccgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGcc                                                      <  2:4481641/93‑1 (MQ=255)

                                      cccgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCaa                                                    <  2:7294946/92‑1 (MQ=255)

                                      accgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCaa                                                    <  1:5885606/93‑1 (MQ=255)

                                      accgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCaa                                                    <  1:904951/93‑1 (MQ=255)

                                        cgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACg                                                  <  2:791278/93‑1 (MQ=255)

                                        cgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACg                                                  >  1:4454151/1‑93 (MQ=255)

                                         gtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACgg                                                 <  1:4622007/93‑1 (MQ=255)

                                         gtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACgg                                                 <  1:7568794/93‑1 (MQ=255)

                                            cccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAg                                              <  2:6377731/92‑1 (MQ=255)

                                            accgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAg                                              <  1:537174/93‑1 (MQ=255)

                                             ccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgt                                             <  1:3069417/93‑1 (MQ=255)

                                             ccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgt                                             >  1:3335600/1‑93 (MQ=255)

                                             ccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgt                                             >  2:5800613/1‑93 (MQ=255)

                                              cgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgtt                                            <  1:5703808/93‑1 (MQ=255)

                                              cgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgtt                                            <  1:4369501/93‑1 (MQ=255)

                                                gCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑T‑‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgttgtt                                         <  1:6875486/93‑1 (MQ=255)

                                                 cacaACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgttgtt                                         <  1:7196789/93‑1 (MQ=255)

                                                   caACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTtgttgt                                       <  1:7460862/93‑1 (MQ=255)

                                                   caACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTtgttgt                                       <  2:5410452/93‑1 (MQ=255)

                                                   caACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTtgttgt                                       <  1:7131153/93‑1 (MQ=255)

                                                   caACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTtgttgt                                       <  2:3534346/93‑1 (MQ=255)

                                                   caACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTtgttgt                                       <  2:3812526/93‑1 (MQ=255)

                                                    aaCCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTa                                      >  1:6236020/1‑93 (MQ=255)

                                                    aaCCAATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTTAATGACTGCCAACGGGAGTTGTTGTa                                      <  2:2956969/93‑1 (MQ=255)

                                                      ccGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGc                                    <  2:1382945/93‑1 (MQ=255)

                                                       cGATCCGCCAAAAAGAGGCGTACCAATGTCGATATC‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCt                                   <  1:5835222/93‑1 (MQ=255)

                                                       cGATCCGCCAAAAAGAGGCGGACCAATGTCGATATA‑GCC‑‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGGTGGTGTAGCt                                   >  1:847472/1‑93 (MQ=255)

                                                        gATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtg                                  >  2:1453620/1‑93 (MQ=255)

                                                        gATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtg                                  >  2:5278641/1‑93 (MQ=255)

                                                         aTCCACCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtgt                                 <  1:1161308/93‑1 (MQ=255)

                                                          tCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtgtg                                <  2:6972657/93‑1 (MQ=255)

                                                           ccGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTgg                               <  2:2868372/93‑1 (MQ=255)

                                                            cGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTggg                              >  1:3796049/1‑93 (MQ=255)

                                                             gCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGgc                             >  1:3420550/1‑93 (MQ=255)

                                                             gCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGgc                             >  1:7604957/1‑93 (MQ=255)

                                                             gCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGgc                             >  1:4580426/1‑93 (MQ=255)

                                                              ccAAAAAGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGgcg                            >  1:651800/1‑93 (MQ=255)

                                                                aaaaaGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGca                          <  1:735976/93‑1 (MQ=255)

                                                                  aaaGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcacc                        >  1:4063855/1‑91 (MQ=255)

                                                                   aaGAGGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaaca                       >  2:5898114/1‑93 (MQ=255)

                                                                    agagGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaacaa                      >  1:6798683/1‑93 (MQ=255)

                                                                    agagGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaacaa                      >  2:275812/1‑93 (MQ=255)

                                                                     gagGCGTACCAATGTCGATATA‑G‑T‑‑CTCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCaacaac                     >  2:6092813/1‑93 (MQ=255)

                                                                      agGCGTACCAATGTCGATATA‑G‑TCCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCaacaac                     >  2:5643978/1‑93 (MQ=255)

                                                                      agGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAacaaca                    <  2:5006249/93‑1 (MQ=255)

                                                                      agGCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAacaaca                    <  2:2087255/93‑1 (MQ=255)

                                                                       ggCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACAt                   >  1:723933/1‑93 (MQ=255)

                                                                       ggCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACAt                   <  2:6452309/93‑1 (MQ=255)

                                                                        gCGTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATg                  >  2:1547662/1‑93 (MQ=255)

                                                                          gTACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGcc                <  2:4437092/93‑1 (MQ=255)

                                                                           tACCAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGccc               >  1:2343289/1‑93 (MQ=255)

                                                                             ccAATGTCGATATA‑G‑TC‑C‑CCCCGCGCTGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCag             <  2:4124414/93‑1 (MQ=255)

                                                                             ccAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCag             >  2:6684280/1‑93 (MQ=255)

                                                                             ccAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCag             >  1:2570928/1‑93 (MQ=255)

                                                                              cAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCaga            >  1:134190/1‑93 (MQ=255)

                                                                              cAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCaga            <  1:4161821/93‑1 (MQ=255)

                                                                              cAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCaga            <  2:3441782/93‑1 (MQ=255)

                                                                              cAATGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCaga            >  2:1748152/1‑93 (MQ=255)

                                                                               aaTGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAGCGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCagag           >  1:3627213/1‑93 (MQ=255)

                                                                                aTGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGc          <  2:263743/93‑1 (MQ=255)

                                                                                 tGTCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCt         >  1:2043589/1‑93 (MQ=255)

                                                                                   tCGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCttt       >  1:2610941/1‑93 (MQ=255)

                                                                                    cGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTa      <  1:4899612/93‑1 (MQ=255)

                                                                                    cGATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTGGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTa      <  1:3678710/93‑1 (MQ=255)

                                                                                     gATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAc     >  2:5985313/1‑93 (MQ=255)

                                                                                     gATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAc     <  1:2926614/93‑1 (MQ=255)

                                                                                     gATATA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAc     >  2:239753/1‑93 (MQ=255)

                                                                                      atatA‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAcc    <  2:7498217/93‑1 (MQ=255)

                                                                                       tata‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCa   >  1:6006687/1‑93 (MQ=255)

                                                                                       tata‑G‑TC‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCa   >  1:1002528/1‑93 (MQ=255)

GCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATA‑G‑T‑‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAA  >  NC_000913/2563488‑2563667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`