BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,044	+G	coding (498/1458 nt)	ascF →	PTS system enzyme IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,043	1	.	G	99.3%	893.9 / ‑1.9	284	coding (497/1458 nt)	ascF	PTS system enzyme IIBC
Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (145/137); total (147/137)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 308 total aligned reads displayed.

GATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑G‑CATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGA  >  NC_000913/2837951‑2838132

gaTGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc                                                                                             >  1:1536322/1‑93 (MQ=255)

  tGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑gg                                                                                          >  1:7433067/1‑92 (MQ=255)

  tGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑gg                                                                                          >  2:5736246/1‑92 (MQ=255)

  tGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑gg                                                                                          >  2:6843689/1‑92 (MQ=255)

     gcggcgTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑ggcat                                                                                       <  2:7261558/93‑5 (MQ=255)

       ggcgTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCt                                                                                     <  2:814536/93‑1 (MQ=255)

        gcgTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTg                                                                                    <  1:6780536/93‑1 (MQ=255)

          gTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGcc                                                                                  >  2:1510136/1‑93 (MQ=255)

           tGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCcg                                                                                 >  1:2609718/1‑93 (MQ=255)

           tGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCcg                                                                                 >  1:1393176/1‑93 (MQ=255)

             cTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCcgcc                                                                               >  2:6547978/1‑92 (MQ=255)

              tGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCcgcca                                                                              >  1:5478821/1‑91 (MQ=255)

                aCAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCcgccatc                                                                            >  2:3037460/1‑89 (MQ=255)

                aCAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCcgccatc                                                                            <  1:1161103/93‑5 (MQ=255)

                 cAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCa                                                                          <  2:5243863/93‑1 (MQ=255)

                 cAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCa                                                                          >  2:6468134/1‑93 (MQ=255)

                  aaaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCaa                                                                         >  1:2014227/1‑93 (MQ=255)

                   aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCaaa                                                                        >  2:7706669/1‑93 (MQ=255)

                    aaGGATCGCCGACCTTAACCATTCTGAATGTTATTGGTGACGGTTCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAAt                                                                       <  1:1982729/93‑1 (MQ=255)

                    aaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAAt                                                                       >  2:5635217/1‑93 (MQ=255)

                    aaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAAt                                                                       >  1:998661/1‑93 (MQ=255)

                    aaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAAt                                                                       <  1:7035886/93‑1 (MQ=255)

                            ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAAcc                                                               >  2:2519221/1‑93 (MQ=255)

                            ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAAcc                                                               <  1:3214121/93‑1 (MQ=255)

                              gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCaa                                                             >  1:7631040/1‑93 (MQ=255)

                                ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAata                                                           <  1:4153702/93‑1 (MQ=255)

                                ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAata                                                           >  2:5465033/1‑93 (MQ=255)

                                ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAata                                                           <  1:7294130/93‑1 (MQ=255)

                                 cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAatat                                                          <  1:4338670/93‑1 (MQ=255)

                                 cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAatat                                                          >  2:4620136/1‑93 (MQ=255)

                                 cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCGGCCGC‑CATCAAATTTAAAACCAatat                                                          <  2:1856515/93‑1 (MQ=255)

                                  ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                         <  1:4245527/93‑1 (MQ=255)

                                  ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                         >  1:2801438/1‑93 (MQ=255)

                                  ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                         >  2:338414/1‑93 (MQ=255)

                                   tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                                        <  2:2243634/93‑1 (MQ=255)

                                   tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                                        <  2:2882166/93‑1 (MQ=255)

                                   tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                                        <  2:3273202/93‑1 (MQ=255)

                                    aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTc                                                       <  1:1349175/93‑1 (MQ=255)

                                     aCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCg                                                      >  1:2044375/1‑93 (MQ=255)

                                      ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                                     <  2:3900780/93‑1 (MQ=255)

                                      ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                                     <  1:6468135/93‑1 (MQ=255)

                                       cATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCt                                                    >  1:5683492/1‑93 (MQ=255)

                                        aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                   >  2:4472772/1‑93 (MQ=255)

                                        aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                   <  2:6903103/93‑1 (MQ=255)

                                         ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTgg                                                  >  2:455282/1‑93 (MQ=255)

                                          tCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                 <  2:4071315/93‑1 (MQ=255)

                                          tCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                 <  2:4899889/93‑1 (MQ=255)

                                          tCTGAATGGGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                 <  1:3503975/93‑1 (MQ=255)

                                            tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGa                                               <  1:3330569/93‑1 (MQ=255)

                                            tGAATGTGATGGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGa                                               <  2:7462596/93‑1 (MQ=255)

                                             gAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAt                                              <  2:1072905/93‑1 (MQ=255)

                                              aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAtt                                             >  1:2582036/1‑93 (MQ=255)

                                                   gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                        <  1:3784256/93‑1 (MQ=255)

                                                   gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                        <  2:2144348/93‑1 (MQ=255)

                                                   gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                        <  2:4860040/93‑1 (MQ=255)

                                                   gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                        >  1:3593201/1‑93 (MQ=255)

                                                    aTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATc                                       >  2:1720413/1‑93 (MQ=255)

                                                      tGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgc                                     >  1:4707666/1‑93 (MQ=255)

                                                         tGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCTGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCggg                                  <  2:6055463/93‑1 (MQ=255)

                                                         tGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCggg                                  <  1:7355054/93‑1 (MQ=255)

                                                          gACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgt                                 <  1:2539987/93‑1 (MQ=255)

                                                          gACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgt                                 <  2:5980527/93‑1 (MQ=255)

                                                            cGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtgt                               >  2:1115331/1‑93 (MQ=255)

                                                             ggTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGtgtg                              <  1:5990443/93‑1 (MQ=255)

                                                             ggTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGACATCGCGGGtgtg                              >  1:4535765/1‑93 (MQ=255)

                                                              gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                             >  2:6700650/1‑93 (MQ=255)

                                                              gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                             >  2:7891932/1‑93 (MQ=255)

                                                              gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                             >  1:5315765/1‑93 (MQ=255)

                                                              gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                             >  1:1215474/1‑93 (MQ=255)

                                                               tGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCt                            <  1:7813441/93‑1 (MQ=255)

                                                               tGCTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCt                            <  1:5078733/93‑1 (MQ=255)

                                                                 cTTTCTTCTTCCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTgg                          <  2:6070335/93‑1 (MQ=255)

                                                                   ttcttcttcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTa                        >  2:4356123/1‑93 (MQ=255)

                                                                    tcttcttcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTAc                       <  1:6192994/93‑1 (MQ=255)

                                                                    tctactccCTGCCGCTGATGCTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTAc                       <  2:3744605/93‑1 (MQ=255)

                                                                     cttcttcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACa                      >  2:4974840/1‑93 (MQ=255)

                                                                       tcttcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                    >  2:4320668/1‑93 (MQ=255)

                                                                       tctccCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAACCCAATATGTCGCTGCCGATTGCCATCGCGGGTGTGCTGGTACATc                    <  2:7583665/93‑1 (MQ=255)

                                                                        cttcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATcc                   >  1:2351194/1‑93 (MQ=255)

                                                                         ttcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCg                  >  1:5609273/1‑93 (MQ=255)

                                                                         ttcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGGGTGCTGGTACATCCg                  >  1:683938/1‑93 (MQ=255)

                                                                          tcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGa                 <  2:1311064/93‑1 (MQ=255)

                                                                          tcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGa                 >  1:2128054/1‑93 (MQ=255)

                                                                          tcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGa                 >  1:2938014/1‑93 (MQ=255)

                                                                          tcCTGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGa                 <  2:1932388/93‑1 (MQ=255)

                                                                            cTGCCGCTGATGGTCGC‑GTCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATGGCCATCGCGGGTGTGCTGGTACATCCGAGt               <  1:1125031/93‑1 (MQ=255)

                                                                             tGCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt              >  2:3605742/1‑93 (MQ=255)

                                                                              gCCGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGttt             >  1:5799421/1‑93 (MQ=255)

                                                                                cGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa           >  1:2479887/1‑93 (MQ=255)

                                                                                cGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa           >  1:7572568/1‑93 (MQ=255)

                                                                                cGCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa           >  1:3993655/1‑93 (MQ=255)

                                                                                 gCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAt          <  2:6243746/93‑1 (MQ=255)

                                                                                 gCTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAt          <  1:3131780/93‑1 (MQ=255)

                                                                                  cTGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAtt         >  1:694052/1‑93 (MQ=255)

                                                                                   tGATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg        >  1:4930811/1‑93 (MQ=255)

                                                                                    gATGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa       >  2:2970888/1‑93 (MQ=255)

                                                                                     aTGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGaa      <  2:3538122/93‑1 (MQ=255)

                                                                                      tGGTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAAc     >  1:1017310/1‑93 (MQ=255)

                                                                                       ggTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACt    >  1:1850509/1‑93 (MQ=255)

                                                                                        gTCGCGG‑CATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTg   >  1:1747229/1‑93 (MQ=255)

GATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑G‑CATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGA  >  NC_000913/2837951‑2838132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`