BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,115,116	+C	coding (2013/2028 nt)	b2974 ←	putative endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,115,115	1	.	C	99.4%	1048.0 / ‑2.1	330	coding (2014/2028 nt)	b2974	putative endoglucanase
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (167/161); total (168/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 354 total aligned reads displayed.

GTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115023‑3115202

 tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑c                                                                                        >  1:5551314/1‑93 (MQ=255)

   tGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCTCATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑cct                                                                                      <  1:4773257/93‑3 (MQ=255)

     cTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑cctgc                                                                                    <  2:1407604/93‑5 (MQ=255)

     cTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑cctgc                                                                                    <  1:442324/93‑5 (MQ=255)

     cTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑cctgc                                                                                    >  1:1315223/1‑89 (MQ=255)

        gTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGa                                                                                  <  2:828179/93‑1 (MQ=255)

         tATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGAt                                                                                 <  1:7174818/93‑1 (MQ=255)

          aTCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATc                                                                                >  1:389584/1‑93 (MQ=255)

           tCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCg                                                                               >  1:5226039/1‑93 (MQ=255)

           tCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCg                                                                               >  2:2640533/1‑93 (MQ=255)

             tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCgag                                                                             <  1:4334691/93‑1 (MQ=255)

               cACGCAGCGGGATTGCATAAGGATCGTTACCCGCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGAt                                                                           <  2:4983396/93‑1 (MQ=255)

               accGCAGCGGGGTTGCATAAGGATCACTACCCACCTGAGTCCCATATTCAAAGCCGTTAAGGATCAGCAGGGGCA‑T‑TTCCTGGGGATCGAGAt                                                                           <  1:4624921/91‑1 (MQ=255)

                ccGCAGCGGGATGGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGGTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATc                                                                          <  2:5988953/92‑1 (MQ=255)

                aCGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATc                                                                          <  1:3005529/93‑1 (MQ=255)

                   cAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGca                                                                       >  2:3750588/1‑92 (MQ=255)

                    cgCGGGATTTCATAAGGATCGTTACCCACCTGGGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCa                                                                      <  2:1930411/92‑1 (MQ=255)

                     gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCAt                                                                     <  1:7717419/93‑1 (MQ=255)

                     gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCAt                                                                     >  1:329250/1‑93 (MQ=255)

                     gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCAt                                                                     <  2:91688/93‑1 (MQ=255)

                       gggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAg                                                                   >  1:929516/1‑93 (MQ=255)

                        ggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGc                                                                  >  1:2624655/1‑93 (MQ=255)

                        ggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGc                                                                  >  2:6422039/1‑93 (MQ=255)

                        ggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGc                                                                  >  2:767607/1‑93 (MQ=255)

                         gATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCt                                                                 >  2:4165452/1‑93 (MQ=255)

                          aTTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTa                                                                >  2:106869/1‑93 (MQ=255)

                          aTTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTa                                                                <  1:120170/93‑1 (MQ=255)

                          aTTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTa                                                                >  2:7352986/1‑93 (MQ=255)

                          aTTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTa                                                                >  1:2489520/1‑93 (MQ=255)

                           ttGCTTAAGGATCGTTACCCACCCGAGTCACATATTCAAAGCCGGTAAGGATCAGCAGCGGCAGG‑AT‑CCGCGGATCGAGATCGTCATAGCTAc                                                               >  2:7039955/1‑93 (MQ=255)

                           ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTAc                                                               >  2:2786052/1‑93 (MQ=255)

                            tGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACt                                                              <  1:669704/93‑1 (MQ=255)

                             gCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACtt                                                             <  2:183503/93‑1 (MQ=255)

                               aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  1:6277558/1‑93 (MQ=255)

                               aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  1:3732115/1‑93 (MQ=255)

                               aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  1:3206272/1‑93 (MQ=255)

                               aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  2:567456/1‑93 (MQ=255)

                                tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  1:5340694/1‑93 (MQ=255)

                                tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          <  1:1486945/93‑1 (MQ=255)

                                tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  1:7590741/1‑93 (MQ=255)

                                   ggATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGc                                                       <  2:6872883/93‑1 (MQ=255)

                                   ggATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGc                                                       >  1:7370642/1‑93 (MQ=255)

                                     aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCta                                                     >  2:200091/1‑92 (MQ=255)

                                     aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     <  2:692458/93‑1 (MQ=255)

                                     aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     <  1:3518266/93‑1 (MQ=255)

                                     aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     >  1:6507245/1‑93 (MQ=255)

                                      tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           <  1:3615303/86‑1 (MQ=255)

                                      tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  2:3615302/1‑86 (MQ=255)

                                      tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCa                                                    <  1:2553292/93‑1 (MQ=255)

                                      tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCa                                                    >  1:2170702/1‑93 (MQ=255)

                                        gtgaCCCACCTCAGACACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAAAGCGCCATAGCTACTTAAATGCTCAAc                                                  <  2:3356993/90‑1 (MQ=255)

                                        gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                  >  1:5030960/1‑93 (MQ=255)

                                        gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                  <  1:3925362/93‑1 (MQ=255)

                                        gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                  >  1:2126780/1‑93 (MQ=255)

                                          tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                >  1:3800916/1‑93 (MQ=255)

                                          tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                <  1:6422040/93‑1 (MQ=255)

                                           aCCCACCTGAGTCACATATTTAAAGCCGGTAAGGGTCAGAAGTGGCA‑T‑TTCCTGCGGGTCGAGATCGCCGTCGCTAGTTAAATGCTCAACata                                               >  1:3190307/1‑91 (MQ=255)

                                           aCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                               <  2:5052424/93‑1 (MQ=255)

                                           aCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                               <  2:4076661/93‑1 (MQ=255)

                                           aCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                               <  1:1715337/93‑1 (MQ=255)

                                             cccccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             <  1:5933117/90‑1 (MQ=255)

                                             ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             >  2:7764892/1‑93 (MQ=255)

                                             ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             <  2:5174560/93‑1 (MQ=255)

                                               cccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATg                                           <  2:5582377/92‑1 (MQ=255)

                                               aCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATg                                           >  2:2985179/1‑93 (MQ=255)

                                                catGAGTCACATATACAAAGCCGTTAAGCATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGc                                          <  2:1282210/91‑1 (MQ=255)

                                                 cTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGcc                                         <  2:6237333/93‑1 (MQ=255)

                                                 cTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGcc                                         >  2:5735538/1‑93 (MQ=255)

                                                 cTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGcc                                         >  1:547343/1‑93 (MQ=255)

                                                   gAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGACATAGCTACTTAAATGCTCAACAGAGATGCCCg                                       >  2:3879779/1‑93 (MQ=255)

                                                    aGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGc                                      >  1:1982102/1‑93 (MQ=255)

                                                      tCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaa                                    >  2:2733367/1‑93 (MQ=255)

                                                      tCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATACCCGCaa                                    <  1:7055291/93‑1 (MQ=255)

                                                        acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  <  2:3130209/93‑1 (MQ=255)

                                                         caTATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAt                                 <  2:5388355/93‑1 (MQ=255)

                                                          atatTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATc                                >  2:7163122/1‑93 (MQ=255)

                                                           tatTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATcc                               >  2:2868384/1‑93 (MQ=255)

                                                           tatTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATcc                               >  2:7865241/1‑93 (MQ=255)

                                                              tCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGa                            >  2:1660029/1‑93 (MQ=255)

                                                                 aaGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                         >  1:3345660/1‑93 (MQ=255)

                                                                  aGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGa                        >  2:1315230/1‑93 (MQ=255)

                                                                   gCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                       >  1:1095818/1‑93 (MQ=255)

                                                                   gCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                       >  1:2681659/1‑93 (MQ=255)

                                                                    ccGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAg                      <  2:6799598/93‑1 (MQ=255)

                                                                     cGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGt                     >  1:7520053/1‑93 (MQ=255)

                                                                      gTTAAGGATCAGCAGCGTCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                    <  1:4676095/93‑1 (MQ=255)

                                                                       ttAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  2:4582528/1‑93 (MQ=255)

                                                                       ttAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  2:2502991/1‑93 (MQ=255)

                                                                         aaGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                 <  2:4390441/93‑1 (MQ=255)

                                                                         aaGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                 <  2:5959792/93‑1 (MQ=255)

                                                                          aGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                >  2:2203955/1‑93 (MQ=255)

                                                                             aTCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  2:7296612/93‑1 (MQ=255)

                                                                             aTCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  1:6656181/93‑1 (MQ=255)

                                                                              tCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAg            >  1:6929628/1‑93 (MQ=255)

                                                                               cagcagCGGCA‑TGTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgc           <  2:48209/93‑1 (MQ=255)

                                                                                 gcagcGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcgc         <  2:4636619/93‑1 (MQ=255)

                                                                                 gcagcGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcgc         >  1:581365/1‑93 (MQ=255)

                                                                                   agcGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTg       >  2:1596147/1‑93 (MQ=255)

                                                                                     cGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGtt     <  1:4584054/93‑1 (MQ=255)

                                                                                       gCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTc   >  2:2338069/1‑93 (MQ=255)

GTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115023‑3115202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`