BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,219,876	G→A	G257D (GGC→GAC)	ebgR →	EbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,219,876	0	G	A	99.7%	1000.2 / ‑5.6	289	G257D (GGC→GAC)	ebgR	EbgR
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (155/131); total (156/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.08e-01

Only 100 of 298 total aligned reads displayed.

AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCA  >  NC_000913/3219786‑3219965

aaaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGAcc                                                                                         <  2:4461017/93‑1 (MQ=255)

aaaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGAcc                                                                                         <  1:2469601/93‑1 (MQ=255)

 aaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCt                                                                                        <  2:5751853/93‑1 (MQ=255)

 aaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCt                                                                                        <  2:493386/93‑1 (MQ=255)

    gCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGaa                                                                                     <  2:3715817/93‑1 (MQ=255)

     cTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAAc                                                                                    >  2:1324122/1‑93 (MQ=255)

      gggCGCGGGAAGACTATGCTAAGGCACTGTCTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATGCATGAACGAGACCTGAACa                                                                                   <  1:4081659/92‑1 (MQ=255)

        gcgcGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATc                                                                                 <  1:1823450/93‑1 (MQ=255)

         cgcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATcc                                                                                >  2:2158472/1‑93 (MQ=255)

           cgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCca                                                                              <  1:1188340/93‑1 (MQ=255)

            gggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcac                                                                             >  2:1570391/1‑93 (MQ=255)

            gggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcac                                                                             >  2:2824918/1‑93 (MQ=255)

            gggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcac                                                                             <  2:2319087/93‑1 (MQ=255)

             ggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcaca                                                                            >  2:4537858/1‑93 (MQ=255)

             ggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcaca                                                                            >  2:2550232/1‑93 (MQ=255)

              gAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAg                                                                           >  1:4995199/1‑93 (MQ=255)

                aGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGa                                                                         >  2:4335871/1‑93 (MQ=255)

                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        <  1:2326884/93‑1 (MQ=255)

                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        >  2:74002/1‑93 (MQ=255)

                  aCTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCCATTCATGAACGAGACCTGAACATCCCACAGGata                                                                       >  1:6847569/1‑93 (MQ=255)

                  aCTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGata                                                                       <  2:1014639/93‑1 (MQ=255)

                  aCTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGata                                                                       <  2:6272405/93‑1 (MQ=255)

                    tATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAtt                                                                     <  1:6582937/93‑1 (MQ=255)

                    tATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAtt                                                                     >  2:667934/1‑93 (MQ=255)

                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   <  2:4152703/93‑1 (MQ=255)

                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   >  2:5236446/1‑93 (MQ=255)

                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   <  1:2756305/93‑1 (MQ=255)

                         gAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCt                                                                <  1:5393023/93‑1 (MQ=255)

                          aaGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               <  1:4614827/93‑1 (MQ=255)

                          aaGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               <  1:3173816/93‑1 (MQ=255)

                           aGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTa                                                              <  1:4929518/93‑1 (MQ=255)

                            ggCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAt                                                             >  2:6978599/1‑93 (MQ=255)

                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  2:7567058/93‑1 (MQ=255)

                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  1:1428657/93‑1 (MQ=255)

                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  1:6246572/93‑1 (MQ=255)

                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  2:4771769/93‑1 (MQ=255)

                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            >  2:7540291/1‑93 (MQ=255)

                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  1:2168068/93‑1 (MQ=255)

                              cACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCa                                                           <  2:1665803/93‑1 (MQ=255)

                              cACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCa                                                           >  1:3071097/1‑93 (MQ=255)

                              cACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCa                                                           <  1:3564178/93‑1 (MQ=255)

                               aCTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  2:5997462/93‑1 (MQ=255)

                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  1:1905320/1‑93 (MQ=255)

                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  2:162065/1‑93 (MQ=255)

                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  1:4524005/1‑93 (MQ=255)

                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  2:3930471/1‑93 (MQ=255)

                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  1:7005024/1‑93 (MQ=255)

                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  1:3415926/1‑93 (MQ=255)

                                  ttttcttGCTTCCGATTCAATTGTTATCCGCATTCTGCGGGCCATTCAGGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGt                                                       <  2:3304286/92‑1 (MQ=255)

                                  gtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGt                                                       >  1:2940001/1‑93 (MQ=255)

                                     tgttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTaa                                                    <  1:7925816/93‑1 (MQ=255)

                                        tgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                                                 >  1:3336831/1‑93 (MQ=255)

                                        tgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                                                 >  2:639199/1‑93 (MQ=255)

                                         gCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGat                                                >  1:3939947/1‑93 (MQ=255)

                                         gCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGaa                                                >  1:4632084/1‑92 (MQ=255)

                                          cTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGata                                               <  2:6546332/93‑1 (MQ=255)

                                          cTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGata                                               <  1:253594/93‑1 (MQ=255)

                                            tCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATc                                             <  2:7223671/93‑1 (MQ=255)

                                              cGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATccc                                           <  1:6658335/93‑1 (MQ=255)

                                               gATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATcccc                                          >  2:3027782/1‑93 (MQ=255)

                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         >  2:3201466/1‑93 (MQ=255)

                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         <  1:3255348/93‑1 (MQ=255)

                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         >  1:7289949/1‑93 (MQ=255)

                                                 ttCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAc                                        <  1:7582568/93‑1 (MQ=255)

                                                    cATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgc                                     >  1:2266932/1‑93 (MQ=255)

                                                    cATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgc                                     <  2:7118892/93‑1 (MQ=255)

                                                      ttGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTCACGATATCCCCACcgcgc                                   >  1:7713142/1‑93 (MQ=255)

                                                      ttGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcgc                                   >  2:6096753/1‑93 (MQ=255)

                                                      ttGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcgc                                   >  1:1361268/1‑93 (MQ=255)

                                                       tGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcgcg                                  >  2:771440/1‑93 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGCtt                               >  2:4075261/1‑93 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:6471018/1‑93 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:3631934/1‑93 (MQ=255)

                                                            tCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTa                             <  2:6945143/93‑1 (MQ=255)

                                                            tCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTa                             >  2:6343342/1‑93 (MQ=255)

                                                             cGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAc                            <  2:1905419/93‑1 (MQ=255)

                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           <  1:4016312/93‑1 (MQ=255)

                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           >  2:5144510/1‑93 (MQ=255)

                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           <  2:975526/93‑1 (MQ=255)

                                                               gCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCt                          >  2:1443192/1‑93 (MQ=255)

                                                                 gTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCttt                        <  1:605678/93‑1 (MQ=255)

                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       >  2:6151324/1‑93 (MQ=255)

                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       >  2:2421148/1‑93 (MQ=255)

                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       >  2:58363/1‑93 (MQ=255)

                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       <  2:5919411/93‑1 (MQ=255)

                                                                   aCTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTcc                      <  1:5677840/93‑1 (MQ=255)

                                                                    cTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccg                     >  2:5497572/1‑93 (MQ=255)

                                                                    cTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccg                     >  1:2705881/1‑93 (MQ=255)

                                                                     tGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccgc                    >  1:4820428/1‑93 (MQ=255)

                                                                        gggCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCcgccgc                 >  1:4398070/1‑93 (MQ=255)

                                                                        gggCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCcgccgc                 >  2:3769084/1‑93 (MQ=255)

                                                                         ggCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGct                >  2:2490638/1‑93 (MQ=255)

                                                                          gCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGctc               >  2:7741102/1‑93 (MQ=255)

                                                                            aaTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCtctc             >  2:1801252/1‑93 (MQ=255)

                                                                            aaTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCtctc             <  2:202709/93‑1 (MQ=255)

                                                                              ttCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCa           >  2:3553101/1‑93 (MQ=255)

                                                                                  tGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGt       >  2:1379040/1‑93 (MQ=255)

                                                                                  tGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGt       <  1:7131236/93‑1 (MQ=255)

                                                                                    aaCGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTgc     >  1:510207/1‑93 (MQ=255)

                                                                                    aaCGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTgc     >  1:1156844/1‑93 (MQ=255)

AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCA  >  NC_000913/3219786‑3219965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`