BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,228,614	G→A	A696T (GCT→ACT)	ygjK →	putative isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,228,614	0	G	A	99.1%	1189.4 / ‑3.5	348	A696T (GCT→ACT)	ygjK	putative isomerase
Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (171/174); total (171/177)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 361 total aligned reads displayed.

GCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGGCTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGTGAT  >  NC_000913/3228524‑3228706

 ccAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACtt                                                                                           <  2:5058870/93‑1 (MQ=255)

  cAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACttt                                                                                          >  1:112307/1‑93 (MQ=255)

  cAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACttt                                                                                          <  2:62690/93‑1 (MQ=255)

     tGCCGACGCGGTGGTGAAGGGGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTgg                                                                                       >  2:4669498/1‑93 (MQ=255)

     tGCCGACGCGGTGGGGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGAGGCCTTAACCAATCCGACTTTTgg                                                                                       >  2:5947871/1‑93 (MQ=255)

         gACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCt                                                                                   <  1:5293424/93‑1 (MQ=255)

          aCGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTg                                                                                  >  1:7481404/1‑93 (MQ=255)

           cgcgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGa                                                                                 <  2:252547/93‑1 (MQ=255)

           cgcgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGa                                                                                 <  2:5114626/93‑1 (MQ=255)

              ggtggtGAAGGTGATGGTCGATCCTAAAGAGGTCAACCCCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGatat                                                                              <  1:4383728/93‑1 (MQ=255)

              ggtggtGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGatat                                                                              >  2:1831185/1‑93 (MQ=255)

               gtggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATc                                                                             <  1:5353454/93‑1 (MQ=255)

               gtggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATc                                                                             <  2:5305178/93‑1 (MQ=255)

                  gtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTAc                                                                          >  1:7155092/1‑93 (MQ=255)

                  gtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTAc                                                                          <  2:4322535/93‑1 (MQ=255)

                    gAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTg                                                                        <  2:6511901/93‑1 (MQ=255)

                    gAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTg                                                                        >  2:2812667/1‑93 (MQ=255)

                    gAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTg                                                                        <  2:7658833/93‑1 (MQ=255)

                     aaGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGTGGGGTTAACCAATCCGACTTTTGGCGCTGATATCTACTgg                                                                       >  1:5518673/1‑93 (MQ=255)

                     aaGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTgg                                                                       <  1:6093217/93‑1 (MQ=255)

                         tGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTg                                                                   <  1:820630/93‑1 (MQ=255)

                             gCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGgcg                                                               <  1:3622584/93‑1 (MQ=255)

                                 gATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                           >  2:1764186/1‑93 (MQ=255)

                                 gATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                           <  1:7766428/93‑1 (MQ=255)

                                  gtCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTAt                                                          <  1:4448948/92‑1 (MQ=255)

                                  aTCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTAt                                                          <  2:1921342/93‑1 (MQ=255)

                                  aTCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTAt                                                          >  2:5563363/1‑93 (MQ=255)

                                  aTCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTAt                                                          >  2:3499321/1‑93 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                         >  1:476664/1‑93 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                         <  2:5219466/93‑1 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                         >  1:6797761/1‑93 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                         <  2:3194458/93‑1 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                         >  1:6034762/1‑93 (MQ=255)

                                    ccTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                        >  1:6924489/1‑93 (MQ=255)

                                    ccTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                        >  2:3012156/1‑93 (MQ=255)

                                    ccTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                        >  1:1537764/1‑93 (MQ=255)

                                    ccTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                        <  1:4091267/93‑1 (MQ=255)

                                    ccTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                        >  2:3479708/1‑93 (MQ=255)

                                     cTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATggg                                                       <  1:1687981/93‑1 (MQ=255)

                                       aaaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTg                                                     <  2:2661566/93‑1 (MQ=255)

                                       aaaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTg                                                     >  1:6619628/1‑93 (MQ=255)

                                       aaaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTg                                                     <  2:659011/93‑1 (MQ=255)

                                       aaaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTg                                                     <  2:5796416/93‑1 (MQ=255)

                                        aaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTgg                                                    >  2:5973568/1‑93 (MQ=255)

                                        aaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTgg                                                    >  2:6430607/1‑93 (MQ=255)

                                           agTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGCTc                                                 >  2:4122934/1‑93 (MQ=255)

                                            gTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCa                                                <  1:7362348/93‑1 (MQ=255)

                                             ttCAGCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAg                                               <  1:196471/93‑1 (MQ=255)

                                             ttCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAg                                               >  2:3672307/1‑93 (MQ=255)

                                              tCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGt                                              >  1:2279365/1‑93 (MQ=255)

                                              tCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGt                                              >  2:1686284/1‑93 (MQ=255)

                                              tCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGt                                              <  2:2176844/93‑1 (MQ=255)

                                               cAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGtt                                             <  1:2634442/93‑1 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                            <  1:781657/93‑1 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                            <  2:1283093/93‑1 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                            <  2:3259061/93‑1 (MQ=255)

                                                 acacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCt                                           <  2:4429657/93‑1 (MQ=255)

                                                 acacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCt                                           >  2:786961/1‑93 (MQ=255)

                                                  cacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTg                                          <  2:4787841/93‑1 (MQ=255)

                                                  cacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGCGGGCGCGTATGGGTGGATCAGTTCTg                                          >  1:2482539/1‑93 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                         >  2:3855185/1‑93 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                         >  2:4063234/1‑93 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                         >  1:210675/1‑93 (MQ=255)

                                                    ccTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGt                                        >  2:2007251/1‑93 (MQ=255)

                                                    ccTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGt                                        <  1:2519699/93‑1 (MQ=255)

                                                     cTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGtt                                       >  1:5891496/1‑93 (MQ=255)

                                                     cTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGtt                                       >  2:6110961/1‑93 (MQ=255)

                                                      tttGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGttt                                      <  2:5477676/93‑1 (MQ=255)

                                                      tttGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGttt                                      >  1:1471169/1‑93 (MQ=255)

                                                       ttGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTg                                     <  1:5200824/93‑1 (MQ=255)

                                                          tCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTc                                  >  1:6344203/1‑93 (MQ=255)

                                                           cccGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCt                                 <  1:6522205/93‑1 (MQ=255)

                                                           cccGCGGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCt                                 <  2:3375079/93‑1 (MQ=255)

                                                             cGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGa                               >  2:572505/1‑93 (MQ=255)

                                                             cGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGa                               <  2:6822525/93‑1 (MQ=255)

                                                              gCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaa                              <  1:3058781/93‑1 (MQ=255)

                                                                tGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAg                            >  1:2415516/1‑93 (MQ=255)

                                                                tGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAg                            >  2:7312479/1‑93 (MQ=255)

                                                                tGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAg                            >  1:6698482/1‑93 (MQ=255)

                                                                  ggAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAggg                          <  2:4522105/93‑1 (MQ=255)

                                                                   gAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGa                         <  1:3990789/93‑1 (MQ=255)

                                                                       ggcggcGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGa                     <  2:4606457/93‑1 (MQ=255)

                                                                       ggcggcGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGa                     <  1:1264235/93‑1 (MQ=255)

                                                                          ggcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCg                  >  1:2823861/1‑93 (MQ=255)

                                                                           gcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGt                 >  2:7489323/1‑93 (MQ=255)

                                                                           gcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGt                 >  2:1466145/1‑93 (MQ=255)

                                                                             gTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTa               >  1:316209/1‑93 (MQ=255)

                                                                             gTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTa               >  1:3232600/1‑93 (MQ=255)

                                                                              ttAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTAc              >  1:1874706/1‑93 (MQ=255)

                                                                               tAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACg             <  1:4974974/93‑1 (MQ=255)

                                                                                aaCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACgg            >  1:3907557/1‑93 (MQ=255)

                                                                                aaCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACgg            >  1:969642/1‑93 (MQ=255)

                                                                                 aCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGt           >  1:352244/1‑93 (MQ=255)

                                                                                   cAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTa         <  1:3434096/93‑1 (MQ=255)

                                                                                    aaTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTAt        <  1:5768670/93‑1 (MQ=255)

                                                                                    aaTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGAACAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTAt        <  1:547483/93‑1 (MQ=255)

                                                                                     aTCCGACTTTTGGTGCTGATATCTTCTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATc       <  1:4883246/93‑1 (MQ=255)

                                                                                     aTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATc       >  2:4562372/1‑93 (MQ=255)

                                                                                     aTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATc       >  2:7137184/1‑93 (MQ=255)

                                                                                       ccGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGt     <  2:3962805/93‑1 (MQ=255)

GCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGGCTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGTGAT  >  NC_000913/3228524‑3228706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`