BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,253,781	T→C	E171G (GAG→GGG)	yhaNM ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,253,781	0	T	C	99.2%	813.7 / ‑5.1	260	E171G (GAG→GGG)	yhaNM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (139/119); minor base A (0/2); total (139/121)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC  >  NC_000913/3253690‑3253870

  cgccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGc                                                                                       >  2:5450251/1‑93 (MQ=255)

      gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcaca                                                                                   >  1:651083/1‑93 (MQ=255)

       cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacac                                                                                  <  1:7803305/93‑1 (MQ=255)

       cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacac                                                                                  >  1:883321/1‑93 (MQ=255)

        aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                                                                 <  1:5270675/93‑1 (MQ=255)

        aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                                                                 <  2:4030822/93‑1 (MQ=255)

        aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                                                                 <  1:2110213/93‑1 (MQ=255)

         aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacac                                                                                <  2:823514/93‑1 (MQ=255)

         aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacac                                                                                >  1:519095/1‑93 (MQ=255)

         aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacac                                                                                >  1:2978024/1‑93 (MQ=255)

          aCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacacc                                                                               >  2:2123378/1‑92 (MQ=255)

           cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGc                                                                              >  2:1339717/1‑93 (MQ=255)

            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCCCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             <  1:1809043/93‑1 (MQ=255)

            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCTGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             >  1:6211567/1‑93 (MQ=255)

            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             >  1:2757842/1‑93 (MQ=255)

            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             <  2:7131153/93‑1 (MQ=255)

            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             <  1:483685/93‑1 (MQ=255)

             ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCct                                                                            <  1:4239609/93‑1 (MQ=255)

              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                                                           >  1:1183692/1‑93 (MQ=255)

              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                                                           <  2:351546/93‑1 (MQ=255)

              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                                                           <  2:3717363/93‑1 (MQ=255)

              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                                                           >  1:3248223/1‑93 (MQ=255)

               aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGCAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                                                          >  1:7637074/1‑93 (MQ=255)

               aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                                                          <  2:2407649/93‑1 (MQ=255)

               aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                                                          >  2:7665973/1‑93 (MQ=255)

                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCcttctg                                                                        <  2:1176014/93‑1 (MQ=255)

                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        >  2:3648377/1‑93 (MQ=255)

                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        >  2:7440562/1‑93 (MQ=255)

                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        >  1:3411169/1‑93 (MQ=255)

                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        >  2:4568989/1‑93 (MQ=255)

                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  1:7184843/1‑93 (MQ=255)

                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  2:1598903/1‑93 (MQ=255)

                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  2:1718153/1‑93 (MQ=255)

                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  1:4402945/1‑93 (MQ=255)

                   cATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTggg                                                                      >  2:4232307/1‑93 (MQ=255)

                     ttGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTa                                                                    >  2:1023903/1‑93 (MQ=255)

                      tGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaa                                                                   >  2:4865493/1‑93 (MQ=255)

                      tGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaa                                                                   <  2:4145370/93‑1 (MQ=255)

                      tGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaa                                                                   >  1:6712400/1‑93 (MQ=255)

                       gACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGCCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaaa                                                                  <  2:6613015/93‑1 (MQ=255)

                         cGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAaca                                                                >  1:7366814/1‑93 (MQ=255)

                         cGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAaca                                                                >  1:3300217/1‑93 (MQ=255)

                          gAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacac                                                               >  2:2118332/1‑93 (MQ=255)

                             cTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCCCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacaccac                                                            <  2:6857474/93‑1 (MQ=255)

                              ttCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTACCACACACGCCTGCTGGGTAAacaccaca                                                           >  2:6955931/1‑93 (MQ=255)

                               tCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacaccacac                                                          >  1:3142553/1‑93 (MQ=255)

                                  ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                       >  1:2912148/1‑93 (MQ=255)

                                  ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                       <  1:5609413/93‑1 (MQ=255)

                                  agATCTCAGCCAGCGTC‑GCTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                       <  1:6098927/92‑1 (MQ=255)

                                    aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                     >  2:3968211/1‑93 (MQ=255)

                                     tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt                                                    >  2:2839402/1‑93 (MQ=255)

                                     tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt                                                    >  1:7830144/1‑93 (MQ=255)

                                      ctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTg                                                   >  1:3466862/1‑93 (MQ=255)

                                          gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc                                               >  1:448261/1‑93 (MQ=255)

                                            cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             <  1:7015212/93‑1 (MQ=255)

                                            cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             >  1:584120/1‑93 (MQ=255)

                                            cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             <  1:6358339/93‑1 (MQ=255)

                                            cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             >  2:5313006/1‑93 (MQ=255)

                                             agcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctca                                            >  1:4982342/1‑92 (MQ=255)

                                              gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa                                           <  1:1110960/93‑1 (MQ=255)

                                                 gtcgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGata                                         >  2:1962820/4‑93 (MQ=255)

                                                  tcgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGatat                                        >  2:5259024/3‑93 (MQ=255)

                                                     ttCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGca                                     <  2:2369651/93‑1 (MQ=255)

                                                      tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcac                                    >  2:5999814/1‑93 (MQ=255)

                                                      tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcac                                    <  1:1968922/93‑1 (MQ=255)

                                                      tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcac                                    >  1:3638377/1‑93 (MQ=255)

                                                        tGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACaa                                  >  1:4027763/1‑93 (MQ=255)

                                                         ggAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAAt                                 >  2:4484031/1‑93 (MQ=255)

                                                          gAAAGCACCGTCAGCGGAGACTGTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATg                                >  2:4978130/1‑93 (MQ=255)

                                                           aaaGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGt                               >  2:5026581/1‑93 (MQ=255)

                                                            aaGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGtt                              <  1:5449998/93‑1 (MQ=255)

                                                             aGCTCCGTCAGCGGAGACTCTTACTCGCCACCTGCATCACACGCCTGCTGGGTAAACCCCACACCATCGTGCGTCTCGATAGGCACAATGTTg                             <  2:4167407/93‑1 (MQ=255)

                                                             aGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTg                             >  1:257522/1‑93 (MQ=255)

                                                             aGCACCGTCAGCGGAGACTCTTGCTCGCCCACTGCCACACACGCCTGCTGGGTAAACACCACCCCATCGTGCGTCTCGATATGCACAATGTTg                             <  2:7567602/93‑1 (MQ=255)

                                                              gCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTgg                            <  2:1420905/93‑1 (MQ=255)

                                                              gCACCGTCAGCGGAGACTCTTGCTCGCCCACTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTgg                            <  1:828210/93‑1 (MQ=255)

                                                                gccGTCAGCGGAGGCTCTTGCTCGCCCCCTGCCACACGCGCCTGCTGGGTAAACACCACACCATCGTCCGTCTCGATATGCAAAATGTTGGTa                          <  2:2910664/92‑1 (MQ=255)

                                                                cccGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                          <  1:1688394/92‑1 (MQ=255)

                                                                 ccGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAt                         <  1:2428515/93‑1 (MQ=255)

                                                                 ccGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAt                         <  2:5615810/93‑1 (MQ=255)

                                                                    tCAGCGGAGCCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      <  2:7067595/93‑1 (MQ=255)

                                                                    tCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      >  2:1491841/1‑93 (MQ=255)

                                                                    tCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      >  2:7871285/1‑93 (MQ=255)

                                                                    tCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      >  2:7378726/1‑93 (MQ=255)

                                                                      aGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCccg                    <  2:232006/93‑1 (MQ=255)

                                                                          gagaCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcga                >  2:379203/1‑93 (MQ=255)

                                                                          gagaCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcga                >  1:7236177/1‑93 (MQ=255)

                                                                           agaCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgac               <  1:1933050/93‑1 (MQ=255)

                                                                             aCTGTTGCCCGCCCCCTGCCACACACGCCTGCTGGGTAAACGCCCCGCAATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga             <  2:6369349/93‑1 (MQ=255)

                                                                              ctctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGAt            <  1:2227816/93‑1 (MQ=255)

                                                                              ctctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGAt            <  1:7100842/93‑1 (MQ=255)

                                                                               tctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATg           >  1:4656741/1‑93 (MQ=255)

                                                                               tctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTCGGTATGCCCGCCGACGATg           >  1:7697438/1‑93 (MQ=255)

                                                                                ctggCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg          <  1:1476314/90‑1 (MQ=255)

                                                                                ctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg          >  1:4645782/1‑93 (MQ=255)

                                                                                   gCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGa       <  2:2606211/93‑1 (MQ=255)

                                                                                       gCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacaca   >  2:3236679/1‑93 (MQ=255)

                                                                                       cccccCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacaca   <  2:3569316/92‑1 (MQ=255)

                                                                                       cccccCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacaca   <  1:2011934/92‑1 (MQ=255)

                                                                                       cccccCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGACTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacaca   <  2:6696038/92‑1 (MQ=255)

ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC  >  NC_000913/3253690‑3253870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`