BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,263,134	A→G	V179A (GTG→GCG)	tdcB ←	threonine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,134	0	A	G	99.5%	1159.7 / ‑3.6	370	V179A (GTG→GCG)	tdcB	threonine dehydratase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (177/191); total (178/192)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 378 total aligned reads displayed.

TGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATA  >  NC_000913/3263044‑3263223

tGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATcgcg                                                                                         >  2:3127076/1‑93 (MQ=255)

tGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATcgcg                                                                                         >  2:1770530/1‑93 (MQ=255)

  tACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGtt                                                                                       >  1:5371335/1‑93 (MQ=255)

   aCGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTa                                                                                      >  1:3857797/1‑93 (MQ=255)

   aCGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTa                                                                                      <  2:2366807/93‑1 (MQ=255)

   aCGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTa                                                                                      <  2:4257497/93‑1 (MQ=255)

   aCGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTa                                                                                      >  1:5174416/1‑93 (MQ=255)

    cGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTAt                                                                                     >  1:3357651/1‑93 (MQ=255)

      ccAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCTCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCg                                                                                   <  1:6363332/93‑1 (MQ=255)

      ccAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACAGCCACCACCAATTGGCACAATCGCGTTATca                                                                                   >  1:6796378/1‑92 (MQ=255)

       cAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGa                                                                                  >  2:7387572/1‑93 (MQ=255)

        aaTAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAc                                                                                 >  1:2602264/1‑93 (MQ=255)

        aaTAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAc                                                                                 >  2:5745825/1‑93 (MQ=255)

        aaTAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAc                                                                                 <  1:5583772/93‑1 (MQ=255)

         aTAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAca                                                                                <  1:6449392/93‑1 (MQ=255)

          tAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAcat                                                                               >  2:5405506/1‑93 (MQ=255)

          tAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAcat                                                                               >  2:2876469/1‑93 (MQ=255)

          tAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAcat                                                                               >  1:4420249/1‑93 (MQ=255)

           aaCACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAcatc                                                                              >  2:2656030/1‑93 (MQ=255)

            acacGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAcatca                                                                             <  1:7793996/93‑1 (MQ=255)

            acacGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAcatca                                                                             >  2:2830380/1‑93 (MQ=255)

            acacGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAcatca                                                                             >  1:5406951/1‑93 (MQ=255)

             cacGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGAcatcat                                                                            <  1:5707211/93‑1 (MQ=255)

              acGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAACCCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATa                                                                           <  2:13201/93‑1 (MQ=255)

              acGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATa                                                                           <  1:713390/93‑1 (MQ=255)

                gAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATaga                                                                         >  2:100242/1‑93 (MQ=255)

                gAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATaga                                                                         >  1:3528666/1‑93 (MQ=255)

                    ggTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATc                                                                     <  2:1823484/93‑1 (MQ=255)

                    ggTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATc                                                                     <  1:5928139/93‑1 (MQ=255)

                       cGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTc                                                                  <  1:2922006/93‑1 (MQ=255)

                        gggTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTcc                                                                 >  2:456964/1‑93 (MQ=255)

                        gggTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTcc                                                                 >  1:2690041/1‑93 (MQ=255)

                        gggTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTcc                                                                 >  1:611462/1‑93 (MQ=255)

                        gggTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTcc                                                                 <  2:6219430/93‑1 (MQ=255)

                        gggTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTcc                                                                 >  1:7714628/1‑93 (MQ=255)

                         ggTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCa                                                                <  2:6902461/93‑1 (MQ=255)

                           ttAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATa                                                              >  1:169397/1‑93 (MQ=255)

                           ttAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATa                                                              >  1:4171095/1‑93 (MQ=255)

                            tAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATaa                                                             >  2:951461/1‑93 (MQ=255)

                            tAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATaa                                                             >  2:1220604/1‑93 (MQ=255)

                                aGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTc                                                         >  2:5035257/1‑93 (MQ=255)

                                 gATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTcc                                                        <  2:1507407/93‑1 (MQ=255)

                                  aTTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCa                                                       <  1:6882499/93‑1 (MQ=255)

                                   tttAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAg                                                      >  2:7216377/1‑93 (MQ=255)

                                   tttAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAg                                                      >  1:1883080/1‑93 (MQ=255)

                                    ttAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGa                                                     <  2:7523348/93‑1 (MQ=255)

                                    ttAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAAAGATCTTCCATAATTTCCAGa                                                     >  1:1326805/1‑93 (MQ=255)

                                     tAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGAc                                                    <  1:5737476/93‑1 (MQ=255)

                                     tAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGAc                                                    >  2:4127526/1‑93 (MQ=255)

                                      aaTTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGAcc                                                   >  1:4495641/1‑93 (MQ=255)

                                       aTTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCa                                                  >  2:4952219/1‑93 (MQ=255)

                                       aTTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCa                                                  >  2:7426342/1‑93 (MQ=255)

                                       aTTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCa                                                  >  1:4009670/1‑93 (MQ=255)

                                        ttGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCaa                                                 >  2:3520002/1‑93 (MQ=255)

                                        ttGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCaa                                                 >  1:4250644/1‑93 (MQ=255)

                                         tGCCACCGCAATAGCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAAt                                                <  1:1619704/93‑1 (MQ=255)

                                         tGCCACCGCAATACCAGCAATTAATCCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAAt                                                <  2:7841866/93‑1 (MQ=255)

                                          gCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATc                                               >  2:6392753/1‑93 (MQ=255)

                                             aCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCTACATCATAGAGATCTTCCATAATTTCCAGACCAATCGtt                                            <  2:3865353/93‑1 (MQ=255)

                                             aCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGtt                                            >  2:6051823/1‑93 (MQ=255)

                                               cGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTcc                                          <  1:2825609/93‑1 (MQ=255)

                                                gCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTccc                                         <  2:2148853/93‑1 (MQ=255)

                                                gCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTccc                                         <  1:7221723/93‑1 (MQ=255)

                                                gCAATACCAGCAATTAAACCGCCACCACCAATTAGCACTATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTccc                                         <  2:7014028/93‑1 (MQ=255)

                                                gCAAAACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACTTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTccc                                         <  2:6684299/93‑1 (MQ=255)

                                                  aaTACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTg                                       >  1:7884158/1‑93 (MQ=255)

                                                  aaTACCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTg                                       >  2:5195068/1‑93 (MQ=255)

                                                    aaCCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGc                                     <  1:6631382/92‑1 (MQ=255)

                                                     aCCAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGcc                                    >  2:3775677/1‑93 (MQ=255)

                                                      ccAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCa                                   >  1:6981990/1‑93 (MQ=255)

                                                       cAGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAg                                  <  1:6749877/93‑1 (MQ=255)

                                                        aGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGc                                 >  2:3252252/1‑93 (MQ=255)

                                                        aGCAATTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGc                                 >  1:364768/1‑93 (MQ=255)

                                                           aaTTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAAt                              >  2:5652947/1‑93 (MQ=255)

                                                            aTTAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATc                             >  2:5289957/1‑93 (MQ=255)

                                                             ttAAACCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCa                            >  2:3365724/1‑93 (MQ=255)

                                                                aaCCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACtt                         >  2:194984/1‑93 (MQ=255)

                                                                 aCCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACttt                        <  2:5537909/93‑1 (MQ=255)

                                                                 aCCGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACttt                        <  1:4760262/93‑1 (MQ=255)

                                                                  ccGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTc                       <  1:6569887/93‑1 (MQ=255)

                                                                   caccaccaccaATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCg                      >  2:5682722/3‑93 (MQ=255)

                                                                   cGCCACCACCAATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCg                      <  1:1441837/93‑1 (MQ=255)

                                                                      caccaccaATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGat                   <  2:260242/93‑1 (MQ=255)

                                                                      caccaccaATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGat                   >  2:1657165/1‑93 (MQ=255)

                                                                      caccaccaATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGat                   >  1:4840116/1‑93 (MQ=255)

                                                                         caccaATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatcat                >  2:2011739/1‑93 (MQ=255)

                                                                         caccaATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatcat                <  1:1073838/93‑1 (MQ=255)

                                                                          cccaATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatcatc               <  1:2220434/92‑1 (MQ=255)

                                                                          accaATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatcatc               <  1:3090990/93‑1 (MQ=255)

                                                                            caATTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGt             >  2:773498/1‑93 (MQ=255)

                                                                             aaTTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTa            >  2:6153041/1‑93 (MQ=255)

                                                                             aaTTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTa            <  2:5896584/93‑1 (MQ=255)

                                                                              aTTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTaa           <  1:1516008/93‑1 (MQ=255)

                                                                              aTTGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTaa           >  1:495110/1‑93 (MQ=255)

                                                                               ttGGCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAg          >  1:3203130/1‑93 (MQ=255)

                                                                                  gCACAATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTg       >  1:5285978/1‑93 (MQ=255)

                                                                                   cacaATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTgg      >  2:7933808/1‑93 (MQ=255)

                                                                                    acaATCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTggg     >  2:2231688/1‑93 (MQ=255)

                                                                                       aTCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATa  >  1:7538599/1‑93 (MQ=255)

                                                                                       aTCGCGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATa  <  2:3737621/93‑1 (MQ=255)

TGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATA  >  NC_000913/3263044‑3263223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`