BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,263,328	T→C	V114V (GTA→GTG)	tdcB ←	threonine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,328	0	T	C	99.1%	1071.1 / ‑2.0	343	V114V (GTA→GTG)	tdcB	threonine dehydratase
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (170/170); total (172/171)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 353 total aligned reads displayed.

CATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTT  >  NC_000913/3263238‑3263417

cATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCAc                                                                                         >  1:5390992/1‑93 (MQ=255)

 aTTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACt                                                                                        <  1:5291747/93‑1 (MQ=255)

  tttCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACtt                                                                                       >  1:1004651/1‑93 (MQ=255)

  tttCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACtt                                                                                       >  2:7317964/1‑93 (MQ=255)

  tttCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACtt                                                                                       <  2:1692758/93‑1 (MQ=255)

    tCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTg                                                                                     <  2:6060846/93‑1 (MQ=255)

      gACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGa                                                                                   <  1:1220604/93‑1 (MQ=255)

       aCAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAt                                                                                  >  1:6364393/1‑93 (MQ=255)

        cAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAtt                                                                                 <  2:812648/93‑1 (MQ=255)

         aaTTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAttt                                                                                >  2:6331095/1‑93 (MQ=255)

         aaTTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAttt                                                                                >  2:2558241/1‑93 (MQ=255)

         aaTTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAttt                                                                                >  2:7584296/1‑93 (MQ=255)

          aTTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAtttt                                                                               <  2:2690040/93‑1 (MQ=255)

          aTTTCGCCCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAtttt                                                                               <  1:4952220/93‑1 (MQ=255)

           tttCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTg                                                                              >  1:6080624/1‑93 (MQ=255)

           tttCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTg                                                                              >  2:6006125/1‑93 (MQ=255)

           tttCGCTCACTTTAGCGATAGTGTCGTTGAAGATATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTg                                                                              >  2:1377134/1‑93 (MQ=255)

            ttCGCTCACTTTGGCGATAGTGTGTTTGAAGTTACCACCATGCAGACCGACTTCTGCGGAGTAGTCGCCCGTTGCCGCCACTTGGGATTTTgg                                                                             <  2:1796024/93‑1 (MQ=255)

            ttCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTgg                                                                             <  1:7661859/93‑1 (MQ=255)

             tCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTAGCACGTTGCCGCCCCTTTGGATTTTGgc                                                                            >  1:504709/1‑93 (MQ=255)

               gCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGgcgc                                                                          <  1:1946602/93‑1 (MQ=255)

                cTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCa                                                                         >  1:7336891/1‑93 (MQ=255)

                cTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCa                                                                         <  1:2011740/93‑1 (MQ=255)

                cTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCa                                                                         <  1:3520003/93‑1 (MQ=255)

                 tCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGTAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCAc                                                                        <  2:4470636/93‑1 (MQ=255)

                 tCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCAc                                                                        <  2:3178947/93‑1 (MQ=255)

                 tCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCAc                                                                        >  2:4712806/1‑93 (MQ=255)

                  cACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCAcc                                                                       <  1:5832397/93‑1 (MQ=255)

                   ccTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCt                                                                      <  1:515113/92‑1 (MQ=255)

                    cTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCtt                                                                     <  2:7167780/93‑1 (MQ=255)

                     tttAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCttt                                                                    >  1:2714746/1‑93 (MQ=255)

                      ttAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCtttt                                                                   <  1:1591070/93‑1 (MQ=255)

                      ttAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCtttt                                                                   <  1:4537985/93‑1 (MQ=255)

                       tAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTg                                                                  >  2:4771734/1‑93 (MQ=255)

                       tAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTg                                                                  <  1:5663856/93‑1 (MQ=255)

                       tAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTg                                                                  <  2:2343634/93‑1 (MQ=255)

                         gCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGc                                                                <  2:560105/93‑1 (MQ=255)

                          cGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCa                                                               >  2:6774733/1‑93 (MQ=255)

                           gATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCAt                                                              >  1:5566796/1‑93 (MQ=255)

                           gATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCAt                                                              >  1:3401356/1‑93 (MQ=255)

                           gATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCAt                                                              >  2:4646820/1‑93 (MQ=255)

                           gATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCACACCTTTTGGCAt                                                              >  2:3080531/1‑93 (MQ=255)

                           gATAGTGTCGTTGAAGTTATCACCATGCAGAACGACCTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCAt                                                              <  2:617410/93‑1 (MQ=255)

                            aTAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATc                                                             >  2:7422617/1‑93 (MQ=255)

                             tAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATca                                                            >  2:1209374/1‑93 (MQ=255)

                               gtgtCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATcacc                                                          >  2:1031268/1‑93 (MQ=255)

                                tgtCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATcacca                                                         >  2:3767287/1‑93 (MQ=255)

                                tgtCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATcacca                                                         >  1:6450229/1‑93 (MQ=255)

                                 gtCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATcaccac                                                        <  1:2974810/93‑1 (MQ=255)

                                   cGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACtt                                                      <  2:4825162/93‑1 (MQ=255)

                                   cGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACtt                                                      >  1:5348046/1‑93 (MQ=255)

                                    gTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACttt                                                     >  2:687062/1‑93 (MQ=255)

                                      tGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAc                                                   <  2:3215307/93‑1 (MQ=255)

                                      tGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAc                                                   >  2:6616849/1‑93 (MQ=255)

                                      tGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAc                                                   <  2:5798868/93‑1 (MQ=255)

                                       gAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAcc                                                  >  2:5062463/1‑93 (MQ=255)

                                       gAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAcc                                                  <  2:5253768/93‑1 (MQ=255)

                                        aaGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCg                                                 <  2:2423352/93‑1 (MQ=255)

                                          gTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTc                                               <  1:748042/93‑1 (MQ=255)

                                           ttATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCg                                              >  1:3989714/1‑93 (MQ=255)

                                            tATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGa                                             <  1:3127077/93‑1 (MQ=255)

                                            tATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTGGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTcta                                             <  2:2199921/93‑3 (MQ=255)

                                            tATCACCATGCAGAACGACTTCTGCGGAGTAGGCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGa                                             <  1:2170298/93‑1 (MQ=255)

                                             aTCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGAt                                            >  2:7670578/1‑93 (MQ=255)

                                              tCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGAtc                                           >  2:7754963/1‑92 (MQ=255)

                                                 ccATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAccc                                        >  1:4198714/1‑93 (MQ=255)

                                                 ccATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAccc                                        <  1:172933/93‑1 (MQ=255)

                                                 ccATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAccc                                        >  1:5300319/1‑93 (MQ=255)

                                                  cATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCa                                       >  1:6961156/1‑93 (MQ=255)

                                                    tGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGc                                     >  1:2118511/1‑93 (MQ=255)

                                                     gCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCa                                    <  2:4115657/93‑1 (MQ=255)

                                                     gCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCa                                    <  2:3545256/93‑1 (MQ=255)

                                                     gCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCa                                    >  2:519961/1‑93 (MQ=255)

                                                        gAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATcg                                 >  2:5427069/1‑93 (MQ=255)

                                                          aCGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATcgcg                               <  2:4260295/93‑1 (MQ=255)

                                                            gACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCa                             >  1:2676260/1‑93 (MQ=255)

                                                               ttCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAgca                          >  1:1110099/1‑91 (MQ=255)

                                                               ttCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGga                          <  1:7216378/93‑1 (MQ=255)

                                                                tCTGCGGAGTAGTCGCACGTTGCTGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGgag                         <  2:4677374/93‑1 (MQ=255)

                                                                 cTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGgaga                        <  2:5692810/93‑1 (MQ=255)

                                                                 cTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGgaga                        >  1:1311692/1‑93 (MQ=255)

                                                                  tGCGGAGTAGTCGCACGTTGCCGCCACTTTTGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGagag                       <  1:3887421/93‑1 (MQ=255)

                                                                  tGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGagag                       >  2:2738536/1‑93 (MQ=255)

                                                                  tGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGagag                       <  1:3032714/93‑1 (MQ=255)

                                                                   gCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAgg                      <  1:1549941/93‑1 (MQ=255)

                                                                    cGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAggg                     >  1:581123/1‑93 (MQ=255)

                                                                      gAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGaa                   >  1:6676424/1‑93 (MQ=255)

                                                                      gAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGaa                   >  1:4678914/1‑93 (MQ=255)

                                                                      gAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGaa                   >  1:7818006/1‑93 (MQ=255)

                                                                          agtCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAccc               <  2:444186/93‑1 (MQ=255)

                                                                            tCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCt             <  2:4791496/93‑1 (MQ=255)

                                                                            tCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCt             <  1:1842852/93‑1 (MQ=255)

                                                                              gCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTg           <  1:5195069/93‑1 (MQ=255)

                                                                               cACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTgc          <  2:312358/93‑1 (MQ=255)

                                                                               cACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTgc          <  1:5009868/93‑1 (MQ=255)

                                                                                   ttGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCAt      >  2:1942825/1‑93 (MQ=255)

                                                                                   ttGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCAt      >  2:3425814/1‑93 (MQ=255)

                                                                                     gccgccACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATgg    <  1:6189079/93‑1 (MQ=255)

                                                                                      ccGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGt   <  1:5682723/93‑1 (MQ=255)

                                                                                      ccGCCACTTTGGATTATGGCGCACCTTTTGGCATCACCACTTAACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATAGt   <  2:7043738/93‑1 (MQ=255)

CATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTT  >  NC_000913/3263238‑3263417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`