BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	361,946	A→G	L153P (CTG→CCG)	lacY ←	galactoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	361,946	0	A	G	100.0%	809.7 / NA	259	L153P (CTG→CCG)	lacY	galactoside permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (124/135); total (124/135)

Only 100 of 286 total aligned reads displayed.

AAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCT  >  NC_000913/361856‑362035

aaaaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgc                                                                                         >  2:233920/1‑93 (MQ=255)

aaaaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgc                                                                                         >  2:5233659/1‑93 (MQ=255)

    cGGCGAGGATGAGTGCACGGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGccc                                                                                     <  2:6216723/93‑1 (MQ=255)

     ggCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCa                                                                                    >  2:4214895/1‑93 (MQ=255)

     ggCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCa                                                                                    <  2:3417005/93‑1 (MQ=255)

       cGAGGATGAGTGCACGGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGc                                                                                  <  1:619993/93‑1 (MQ=255)

        gAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGcc                                                                                 >  2:5070087/1‑93 (MQ=255)

         aGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCa                                                                                <  2:5623114/93‑1 (MQ=255)

         aGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCa                                                                                >  2:2981145/1‑93 (MQ=255)

          ggATGAGTGCACGGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCaa                                                                               >  2:1050944/1‑93 (MQ=255)

           gATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAac                                                                              <  1:115507/93‑1 (MQ=255)

             tGAGTGCACGGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAacac                                                                            >  1:4912285/1‑93 (MQ=255)

             tGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAacac                                                                            >  2:821062/1‑93 (MQ=255)

             tGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAacac                                                                            >  2:5986003/1‑93 (MQ=255)

                 tGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGcc                                                                        >  1:215109/1‑93 (MQ=255)

                 tGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGcc                                                                        >  2:6489058/1‑93 (MQ=255)

                    acaGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCaaa                                                                     <  1:6561321/93‑1 (MQ=255)

                    acaGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCaaa                                                                     >  1:580372/1‑93 (MQ=255)

                       gCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACAt                                                                  <  1:7907002/93‑1 (MQ=255)

                        ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATc                                                                 <  2:2839304/93‑1 (MQ=255)

                         cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGGACACGGCGCCCAGCCAACACAGACAAACATcc                                                                >  1:3989303/1‑93 (MQ=255)

                         cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATcc                                                                <  2:7924912/93‑1 (MQ=255)

                           gagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgc                                                              >  2:1658187/1‑93 (MQ=255)

                           gagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgc                                                              >  1:4064333/1‑93 (MQ=255)

                           gagCCCAGCCAGAAAACAAACTGATTATTGATGGTAAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgc                                                              <  2:3219048/93‑1 (MQ=255)

                             gCCCAGACAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgc                                                            <  1:4117659/93‑1 (MQ=255)

                               ccagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCATCCAACACAGCCAAACATCcgcgcgc                                                          <  2:894668/93‑1 (MQ=255)

                               ccagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcgc                                                          <  1:4650839/93‑1 (MQ=255)

                               ccagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcgc                                                          <  2:5390194/93‑1 (MQ=255)

                                  gccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGAc                                                       <  1:7365642/93‑1 (MQ=255)

                                    cagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCa                                                     >  1:45390/1‑93 (MQ=255)

                                     agAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaa                                                    >  1:2640691/1‑93 (MQ=255)

                                     agAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaa                                                    >  1:1675225/1‑93 (MQ=255)

                                      gAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaaa                                                   <  2:1346995/93‑1 (MQ=255)

                                       aaaaCAAACTGATTTTTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAt                                                  <  2:857106/93‑1 (MQ=255)

                                       aaaaCAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAt                                                  <  1:3273059/93‑1 (MQ=255)

                                       aaaaCAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAt                                                  >  1:1443919/1‑93 (MQ=255)

                                       aaaaCAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAt                                                  <  1:7171913/93‑1 (MQ=255)

                                        aaacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAtt                                                 >  1:1280766/1‑93 (MQ=255)

                                        aaacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAtt                                                 >  1:3383357/1‑93 (MQ=255)

                                         aacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTc                                                >  2:6681883/1‑93 (MQ=255)

                                          acaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCg                                               >  1:2828948/1‑93 (MQ=255)

                                           caaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGa                                              <  1:6235599/93‑1 (MQ=255)

                                           caaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGa                                              <  1:4185685/93‑1 (MQ=255)

                                             aacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGaaa                                            >  1:565831/1‑93 (MQ=255)

                                              acTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAAt                                           <  1:1951221/93‑1 (MQ=255)

                                               cTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAAtt                                          >  2:5022092/1‑93 (MQ=255)

                                               cTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAAtt                                          >  2:1966927/1‑93 (MQ=255)

                                                tGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         >  1:1084933/1‑93 (MQ=255)

                                                tGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         >  2:7490248/1‑93 (MQ=255)

                                                tGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         >  2:2112414/1‑93 (MQ=255)

                                                tGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         <  2:3804284/93‑1 (MQ=255)

                                                 gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTAc                                        <  1:6893848/93‑1 (MQ=255)

                                                 gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTAc                                        >  2:7278116/1‑93 (MQ=255)

                                                 gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTAc                                        <  1:2739165/93‑1 (MQ=255)

                                                 gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTAc                                        >  2:3852373/1‑93 (MQ=255)

                                                  attattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACt                                       <  2:4861405/93‑1 (MQ=255)

                                                    tattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGc                                     <  1:3919424/93‑1 (MQ=255)

                                                    tattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGc                                     <  2:1763770/93‑1 (MQ=255)

                                                    tattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGc                                     <  1:2336807/93‑1 (MQ=255)

                                                    tattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGc                                     <  2:3092018/93‑1 (MQ=255)

                                                     attGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCg                                    <  1:4413027/93‑1 (MQ=255)

                                                     attGATGGTGAACATGATGCCGACAATCGAGGCACACGGCACCCAGCCAACACAGCCAAAGATCCGCGCGCGACCAAATTCGCAATTACTggg                                    >  2:5867843/1‑91 (MQ=255)

                                                      ttGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGa                                   <  2:306313/93‑1 (MQ=255)

                                                       tGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGAc                                  <  2:6009432/93‑1 (MQ=255)

                                                        gATGGTGAACATGATGCCGACAATCGAGGCAAACGGCGCCCAGCCAACACAGCCACACATCCGCGCGCGACCAAATTCGAAATTACTGCGACg                                 >  2:678252/1‑93 (MQ=255)

                                                         aTGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACgg                                >  2:4566198/1‑93 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               >  1:1059086/1‑93 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               >  1:7538287/1‑93 (MQ=255)

                                                               aaCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACt                          >  1:7313759/1‑93 (MQ=255)

                                                                aCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACtt                         >  2:5170853/1‑93 (MQ=255)

                                                                 cATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGTGCGACCAAATTCGAAATTACTGCGACGGCTGACttt                        >  2:7602040/1‑93 (MQ=255)

                                                                  atgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtc                       >  1:7494987/1‑93 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      >  1:3087931/1‑93 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      >  1:5132263/1‑93 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      >  1:931413/1‑93 (MQ=255)

                                                                     atgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCa                    <  1:3560246/93‑1 (MQ=255)

                                                                      tgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCaa                   <  1:4298164/93‑1 (MQ=255)

                                                                         cGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaa                <  2:1885281/93‑1 (MQ=255)

                                                                          gACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaaa               <  1:3408066/93‑1 (MQ=255)

                                                                            cAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATg             <  2:221369/93‑1 (MQ=255)

                                                                            cAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATg             >  2:3579750/1‑93 (MQ=255)

                                                                             aaTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGc            >  2:7051105/1‑93 (MQ=255)

                                                                             aaTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGc            <  2:1582348/93‑1 (MQ=255)

                                                                              aTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc           >  1:2927370/1‑93 (MQ=255)

                                                                              aTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc           >  2:7222718/1‑93 (MQ=255)

                                                                               tCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCct          >  2:1478199/1‑93 (MQ=255)

                                                                                cGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctc         <  2:4689353/93‑1 (MQ=255)

                                                                                 gAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctct        >  1:1998592/1‑93 (MQ=255)

                                                                                  aGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTa       <  2:4137491/93‑1 (MQ=255)

                                                                                  aGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTa       <  1:821062/93‑1 (MQ=255)

                                                                                   ggCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCTCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAc      <  1:6512532/93‑1 (MQ=255)

                                                                                   ggCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAc      <  1:1523949/93‑1 (MQ=255)

                                                                                   ggCACACGGCGCCCAGCCAACACAGCCAAAAATCCGCGCGCGACCAAATTCGAAATTAATGCGACGGCTGACTTTCTCAATAAATGCCTCTAc      >  1:5305105/1‑93 (MQ=255)

                                                                                    gCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAct     <  1:6396295/93‑1 (MQ=255)

                                                                                    gCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAct     <  2:3524184/93‑1 (MQ=255)

                                                                                      ccacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgc   <  1:6328384/92‑1 (MQ=255)

                                                                                      acacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgc   >  2:5516271/1‑93 (MQ=255)

                                                                                      acacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgc   <  2:448319/93‑1 (MQ=255)

                                                                                       cacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgct  >  1:5072781/1‑93 (MQ=255)

AAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCT  >  NC_000913/361856‑362035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`