BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,266,308	T→C	S85P (TCT→CCT)	yhaC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,266,308	0	T	C	99.6%	865.5 / ‑5.1	273	S85P (TCT→CCT)	yhaC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (165/107); total (165/108)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

Only 100 of 296 total aligned reads displayed.

ACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAA  >  NC_000913/3266218‑3266397

  gTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCctct                                                                                       <  1:2089028/93‑1 (MQ=255)

    tACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTc                                                                                     <  1:4464489/93‑1 (MQ=255)

       aaCCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTc                                                                                  >  1:1489423/1‑93 (MQ=255)

       aaCCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTc                                                                                  >  1:3484428/1‑93 (MQ=255)

        aCCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCt                                                                                 <  1:1714422/93‑1 (MQ=255)

        aCCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCt                                                                                 <  1:5082991/93‑1 (MQ=255)

        aCCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCt                                                                                 <  1:280402/93‑1 (MQ=255)

         ccTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTg                                                                                >  1:3169662/1‑93 (MQ=255)

           tggtggAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGa                                                                              <  1:6212096/93‑1 (MQ=255)

            ggtggAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGAt                                                                             <  2:760181/93‑1 (MQ=255)

              tggAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGAttt                                                                           >  2:3257605/1‑93 (MQ=255)

              tggAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGAttt                                                                           >  1:7800329/1‑93 (MQ=255)

               ggAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGAtttt                                                                          >  2:1608997/1‑93 (MQ=255)

                 aaaaTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGAtttttt                                                                        >  1:683123/1‑93 (MQ=255)

                  aaaTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTa                                                                       <  2:7803290/93‑1 (MQ=255)

                  aaaTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTa                                                                       <  2:3665976/93‑1 (MQ=255)

                   aaTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAg                                                                      >  1:3679469/1‑93 (MQ=255)

                   aaTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCCTCTCTTCGTCTGGATTTTTTAg                                                                      >  1:6533284/1‑93 (MQ=255)

                    aTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAgg                                                                     >  1:4251378/1‑93 (MQ=255)

                    aTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAgg                                                                     <  2:6718105/93‑1 (MQ=255)

                    aTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAgg                                                                     >  2:5216944/1‑93 (MQ=255)

                      gCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGtt                                                                   >  2:5461218/1‑93 (MQ=255)

                       cATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTta                                                                  <  1:3391007/93‑1 (MQ=255)

                         ttATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTtata                                                                >  2:2546995/1‑93 (MQ=255)

                         ttATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTtata                                                                >  1:1283474/1‑93 (MQ=255)

                         ttATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTtata                                                                >  2:5811267/1‑93 (MQ=255)

                         ttAGCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTtata                                                                >  2:1080166/1‑93 (MQ=255)

                          tATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATaa                                                               >  1:358968/1‑93 (MQ=255)

                           aTCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAAc                                                              <  2:3361135/93‑1 (MQ=255)

                                 ggTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCaa                                                        <  2:4339624/93‑1 (MQ=255)

                                    gATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAatt                                                     <  2:2367121/93‑1 (MQ=255)

                                     aTTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAatta                                                    >  2:6248874/1‑93 (MQ=255)

                                     aTTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAatta                                                    <  1:3010486/93‑1 (MQ=255)

                                       tttCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAattatt                                                  <  1:531257/93‑1 (MQ=255)

                                        ttCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTc                                                 >  1:4827755/1‑93 (MQ=255)

                                        ttCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTc                                                 <  1:2663372/93‑1 (MQ=255)

                                         tCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCa                                                >  2:4143443/1‑93 (MQ=255)

                                         tCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCa                                                <  2:6369752/93‑1 (MQ=255)

                                         tCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCa                                                >  2:3416522/1‑93 (MQ=255)

                                          cAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCaa                                               <  2:5318663/93‑1 (MQ=255)

                                           aaaaaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAAc                                              <  2:2586186/93‑1 (MQ=255)

                                            aaaaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACg                                             <  2:2737198/93‑1 (MQ=255)

                                             aaaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGa                                            >  1:5999384/1‑93 (MQ=255)

                                             aaaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGa                                            <  2:6568875/93‑1 (MQ=255)

                                             aaaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGa                                            <  1:7705674/93‑1 (MQ=255)

                                              aaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGAt                                           >  1:4078795/1‑93 (MQ=255)

                                                gAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCg                                         >  2:3841016/1‑93 (MQ=255)

                                                gAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCg                                         <  1:4053629/93‑1 (MQ=255)

                                                gAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCg                                         >  1:3819722/1‑93 (MQ=255)

                                                 aaTTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCgg                                        >  2:1953397/1‑93 (MQ=255)

                                                  aTTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGa                                       <  1:860954/93‑1 (MQ=255)

                                                  aTTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGa                                       >  1:5863504/1‑93 (MQ=255)

                                                   ttAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGAc                                      >  2:7182877/1‑93 (MQ=255)

                                                       cGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATa                                  >  1:2335421/1‑93 (MQ=255)

                                                        gTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATAc                                 <  2:7461932/93‑1 (MQ=255)

                                                         tACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACt                                >  1:405018/1‑93 (MQ=255)

                                                         tACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACt                                >  2:7155686/1‑93 (MQ=255)

                                                         tACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACt                                >  1:4980389/1‑93 (MQ=255)

                                                          aCTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACtt                               >  1:5381643/1‑93 (MQ=255)

                                                           cTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTtg                              >  1:2610681/1‑93 (MQ=255)

                                                            ttACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTtgt                             <  2:4305772/93‑1 (MQ=255)

                                                             tACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTCGATTTTTTACGTTATAACGCCCAAATTATTCCACGATCGGGCAATACTtgtg                            >  2:5360060/1‑93 (MQ=255)

                                                              aCAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGa                           >  1:1180644/1‑93 (MQ=255)

                                                               cAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGaa                          <  2:180766/93‑1 (MQ=255)

                                                               cAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGaa                          >  2:1227592/1‑93 (MQ=255)

                                                               cAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGaa                          >  2:4249463/1‑93 (MQ=255)

                                                                aaaaTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAAc                         >  2:245301/1‑93 (MQ=255)

                                                                 aaaTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACt                        >  1:330195/1‑93 (MQ=255)

                                                                   aTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTa                      >  2:5636828/1‑93 (MQ=255)

                                                                   aTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTa                      <  1:562390/93‑1 (MQ=255)

                                                                   aTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTa                      >  2:7432855/1‑93 (MQ=255)

                                                                    tGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTAc                     >  1:1954688/1‑93 (MQ=255)

                                                                    agTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTAc                     <  2:6473365/92‑1 (MQ=255)

                                                                     gTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGGCCAAATTATTCAACGATCGCACAATACTTGTGAACTTAcc                    >  1:7055122/1‑93 (MQ=255)

                                                                     gTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTAcc                    <  1:7022777/93‑1 (MQ=255)

                                                                      tAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAACTTATTCAACGATCGGACAATACTTGTGCACTTACCa                   >  1:5969270/1‑93 (MQ=255)

                                                                      tAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCa                   >  1:1087873/1‑93 (MQ=255)

                                                                         ttCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTa                >  2:7081621/1‑93 (MQ=255)

                                                                         ttCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTa                >  2:1302906/1‑93 (MQ=255)

                                                                           cTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAt              <  2:3391255/93‑1 (MQ=255)

                                                                           cTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAt              <  2:3114387/93‑1 (MQ=255)

                                                                             ttATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGa            <  2:4441682/93‑1 (MQ=255)

                                                                             ttATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAAATATTCAACCATCGGACAATACTTTTGTACTTACCATTAATGa            >  1:7146970/1‑93 (MQ=255)

                                                                              tataATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGaa           <  2:398604/93‑1 (MQ=255)

                                                                               ataATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAAc          <  1:395682/93‑1 (MQ=255)

                                                                                taATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAAcc         <  1:7556372/93‑1 (MQ=255)

                                                                                taATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAAcc         >  1:7464377/1‑93 (MQ=255)

                                                                                  aTGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGt       <  2:4624071/93‑1 (MQ=255)

                                                                                  aTGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGt       >  1:224393/1‑93 (MQ=255)

                                                                                   tGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGtt      <  1:1561107/93‑1 (MQ=255)

                                                                                   tGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGtt      >  2:278346/1‑93 (MQ=255)

                                                                                   tGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAAACGtt      >  2:6079842/1‑93 (MQ=255)

                                                                                     tctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaa    >  2:7291274/1‑93 (MQ=255)

                                                                                     tctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaa    <  2:4857009/93‑1 (MQ=255)

                                                                                     tctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaa    >  2:2161464/1‑93 (MQ=255)

                                                                                      ctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaaa   <  1:2436226/93‑1 (MQ=255)

                                                                                      ctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaaa   <  1:7364411/93‑1 (MQ=255)

                                                                                      ctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaaa   >  1:1001732/1‑93 (MQ=255)

                                                                                       tcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaaaa  >  1:7244368/1‑93 (MQ=255)

                                                                                       tcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaaaa  <  2:909728/93‑1 (MQ=255)

ACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAA  >  NC_000913/3266218‑3266397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`