BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,300,625	G→A	V172I (GTC→ATC)	yhbV →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,300,625	0	G	A	99.7%	1066.7 / NA	300	V172I (GTC→ATC)	yhbV	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (160/139); minor base C (0/1); total (160/140)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

TGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACC  >  NC_000913/3300535‑3300714

tgtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATc                                                                                         <  1:1614925/93‑1 (MQ=255)

tgtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATc                                                                                         >  1:4074098/1‑93 (MQ=255)

 gtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATcc                                                                                        >  1:3181473/1‑93 (MQ=255)

  tATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCt                                                                                       <  1:1504930/93‑1 (MQ=255)

  tATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCt                                                                                       <  2:3492752/93‑1 (MQ=255)

   aTGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTg                                                                                      >  2:796928/1‑93 (MQ=255)

    tGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGa                                                                                     <  1:7206640/93‑1 (MQ=255)

      ccAGTGGAGCTCTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGCCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGc                                                                                   <  1:4960753/93‑1 (MQ=255)

       cAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCt                                                                                  <  2:4849698/93‑1 (MQ=255)

        aGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTa                                                                                 <  2:1346198/93‑1 (MQ=255)

        aGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTa                                                                                 >  1:5398172/1‑93 (MQ=255)

        aGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTa                                                                                 <  1:3025606/93‑1 (MQ=255)

         gTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTAc                                                                                <  2:5352453/93‑1 (MQ=255)

          tGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACg                                                                               >  2:5628601/1‑93 (MQ=255)

            gAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGt                                                                             <  2:7394776/93‑1 (MQ=255)

             aGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTTAACTGGAAATCCTGAGCTATGGTc                                                                            >  1:170187/1‑93 (MQ=255)

             aGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTc                                                                            >  1:2775801/1‑93 (MQ=255)

             aGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTc                                                                            >  1:2226043/1‑93 (MQ=255)

              gCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCa                                                                           <  1:2146339/93‑1 (MQ=255)

               cTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCAt                                                                          <  2:2777332/93‑1 (MQ=255)

                tttCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATc                                                                         <  2:3144896/93‑1 (MQ=255)

                tttCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATc                                                                         <  2:2910097/93‑1 (MQ=255)

                tttCCCGCGACTGGCTGGTGAATCAGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATc                                                                         <  2:1473040/93‑1 (MQ=255)

                   cccGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGc                                                                      <  2:422498/93‑1 (MQ=255)

                    ccGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGcc                                                                     <  2:3222591/93‑1 (MQ=255)

                      gcgACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGc                                                                   >  2:5108736/1‑93 (MQ=255)

                       cgACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCt                                                                  >  2:5717389/1‑93 (MQ=255)

                         aCTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTgg                                                                >  2:3423685/1‑93 (MQ=255)

                            ggctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCt                                                             >  2:1537257/1‑93 (MQ=255)

                            ggctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCt                                                             >  2:7104988/1‑93 (MQ=255)

                             gctggTGAATCTGCTTTATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTa                                                            >  2:6290809/1‑93 (MQ=255)

                             gctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTa                                                            <  2:6444099/93‑1 (MQ=255)

                             gctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTa                                                            >  1:5792038/1‑93 (MQ=255)

                             gctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTa                                                            >  2:5498594/1‑93 (MQ=255)

                                ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                         >  2:6102403/1‑93 (MQ=255)

                                ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                         <  1:7672762/93‑1 (MQ=255)

                                ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                         <  1:5514480/93‑1 (MQ=255)

                                 gTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTCAGCTACGGTCATCTGCCGCTGGCCTACTcc                                                        >  2:1690816/1‑93 (MQ=255)

                                  tGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccg                                                       >  2:4810547/1‑93 (MQ=255)

                                  tGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTcac                                                       >  1:6529211/1‑91 (MQ=255)

                                    aaTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcc                                                     <  1:5073203/93‑1 (MQ=255)

                                    aaTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcc                                                     <  1:6896903/93‑1 (MQ=255)

                                      tCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcccg                                                   >  1:6424133/1‑93 (MQ=255)

                                       cTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcccgc                                                  <  2:3961925/93‑1 (MQ=255)

                                        tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                                 >  1:1849424/1‑93 (MQ=255)

                                        tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                                 >  2:5219990/1‑93 (MQ=255)

                                        tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                                 >  1:2370647/1‑93 (MQ=255)

                                         gCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctg                                                >  1:7822441/1‑93 (MQ=255)

                                         gCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctg                                                >  2:5916305/1‑93 (MQ=255)

                                         gCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctg                                                <  2:1887718/93‑1 (MQ=255)

                                          cTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctgc                                               >  2:4685705/1‑93 (MQ=255)

                                           ttAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctgct                                              >  2:2357202/1‑93 (MQ=255)

                                             aaTCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCttt                                            >  1:2896327/1‑93 (MQ=255)

                                             aaTCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCttt                                            <  2:7453864/93‑1 (MQ=255)

                                             aaTCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCttt                                            <  1:6542759/93‑1 (MQ=255)

                                              aTCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTa                                           >  1:4602012/1‑93 (MQ=255)

                                               tCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTAc                                          <  1:3785514/93‑1 (MQ=255)

                                               tCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTAc                                          <  1:851784/93‑1 (MQ=255)

                                               tCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGCAAATCCTCAGCTACTGTCATCTGCCGCTGGCCTACTCCGTCCGCTACTTTAc                                          >  2:4292831/1‑93 (MQ=255)

                                                   tGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACcgcg                                      >  2:7337587/1‑93 (MQ=255)

                                                      gATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGt                                   >  2:7228575/1‑93 (MQ=255)

                                                       aTGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGtt                                  <  1:6721373/93‑1 (MQ=255)

                                                         gAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCg                                >  1:6176487/1‑93 (MQ=255)

                                                         gAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCg                                <  1:312297/93‑1 (MQ=255)

                                                          aGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCgg                               >  2:2125398/1‑93 (MQ=255)

                                                            ctaggCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGaa                             <  1:5965576/90‑1 (MQ=255)

                                                            cTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGaa                             <  1:1354338/93‑1 (MQ=255)

                                                            cTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGaa                             <  1:4834381/93‑1 (MQ=255)

                                                             tGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAg                            >  1:3066224/1‑93 (MQ=255)

                                                              gggCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGa                           <  2:7725386/93‑1 (MQ=255)

                                                               ggCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGAc                          <  1:4384504/93‑1 (MQ=255)

                                                                gCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGAcc                         <  1:3759318/93‑1 (MQ=255)

                                                                 cATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCg                        >  2:2552562/1‑93 (MQ=255)

                                                                 cATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCg                        <  1:2079844/93‑1 (MQ=255)

                                                                   ttCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCTGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGcc                      >  2:6573134/1‑93 (MQ=255)

                                                                   ttCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGcc                      <  1:4008539/93‑1 (MQ=255)

                                                                   ttCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGcc                      <  1:2476597/93‑1 (MQ=255)

                                                                    tCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGccc                     >  2:3205905/1‑93 (MQ=255)

                                                                       tAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGaa                  >  1:1105589/1‑93 (MQ=255)

                                                                         aCCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAg                >  1:3943666/1‑93 (MQ=255)

                                                                          ccAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCTCTGGCCTACTCCGCCCGCTGCTTTCCCGCGAGTTCGGAAGACCGCCCGAAAGa               <  2:3786729/93‑1 (MQ=255)

                                                                          ccAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGa               >  2:4589824/1‑93 (MQ=255)

                                                                          ccAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGa               >  2:4903070/1‑93 (MQ=255)

                                                                            aGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATg             <  1:2332688/93‑1 (MQ=255)

                                                                            aGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATg             <  1:4219982/93‑1 (MQ=255)

                                                                            aGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATg             >  1:5844598/1‑93 (MQ=255)

                                                                            aGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATg             >  2:6204494/1‑93 (MQ=255)

                                                                            aGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATg             <  2:3635754/93‑1 (MQ=255)

                                                                             gTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGa            >  1:5688726/1‑93 (MQ=255)

                                                                             gTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGa            >  2:3063460/1‑93 (MQ=255)

                                                                               ttGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgt          <  2:2295455/93‑1 (MQ=255)

                                                                               ttGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgg          >  2:6663319/1‑92 (MQ=255)

                                                                                 gAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgtgt        <  1:745145/93‑1 (MQ=255)

                                                                                 gAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgtgt        <  2:4080000/93‑1 (MQ=255)

                                                                                   aGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGa      >  2:6825478/1‑93 (MQ=255)

                                                                                   aGTGGAAATCCTGAGCTACGGTAATCTGACGCTGGCCTACTACGCCCGCTGCTTTACCGCGCGATCGGAAGACCGCCCGAAAGATGAGTGTGa      <  1:7017513/93‑1 (MQ=255)

                                                                                    gTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGACAGATGAGTGTGaa     >  1:4433258/1‑93 (MQ=255)

                                                                                    gTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGaa     >  1:7548272/1‑93 (MQ=255)

                                                                                      ggAAATCCTGTGCTGCGGTCATCGGCCGCTGGCCTACTCCGCCCGCTGCTTTACCTCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAAc   <  1:1013252/93‑1 (MQ=255)

                                                                                      ggAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAAc   <  2:6019728/93‑1 (MQ=255)

TGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACC  >  NC_000913/3300535‑3300714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`