Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,304,194 A→G R453R (CGT→CGC)  deaD ← inducible ATP‑independent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,304,1940AG99.7% 1031.4 / ‑5.5 326R453R (CGT→CGC) deaDinducible ATP‑independent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (144/181);  total (144/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 334 total aligned reads displayed.

ACGGTCGTTACGATCGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAG  >  NC_000913/3304104‑3304283
                                                                                          |                                                                                         
   gTCGTTACGATCGCGCGGACCACGGTCATCACGGTCACAGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTc                                                                                      >  2:431458/1‑93 (MQ=255)
     cGTTACGATCGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCAc                                                                                    <  1:5001412/93‑1 (MQ=255)
     cGTTACGATCGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCAc                                                                                    <  1:4797902/93‑1 (MQ=255)
         aCGATCGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCtg                                                                                >  1:1296247/1‑93 (MQ=255)
          cGATCGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCtgt                                                                               <  1:1224924/93‑1 (MQ=255)
           gATCGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCtgtg                                                                              <  1:6675842/93‑1 (MQ=255)
            aTCGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGc                                                                             >  2:3940200/1‑93 (MQ=255)
                cgcgGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCAtt                                                                         >  1:5906631/1‑93 (MQ=255)
                cgcgGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCAtt                                                                         <  2:5078017/93‑1 (MQ=255)
                   ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTc                                                                      <  2:2451257/93‑1 (MQ=255)
                     aCCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGCACGATCAGAGTGCGTTCACCCTGTGCCATTTTcag                                                                    <  2:1057125/93‑1 (MQ=255)
                       cACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTcagca                                                                  <  1:7932731/93‑1 (MQ=255)
                       cACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTcagca                                                                  <  1:4216603/93‑1 (MQ=255)
                        aCGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTcagcag                                                                 <  1:5992462/93‑1 (MQ=255)
                        aCGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTcagcag                                                                 >  2:1087482/1‑93 (MQ=255)
                         cGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGt                                                                <  1:6842265/93‑1 (MQ=255)
                         cGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGt                                                                <  2:5875134/93‑1 (MQ=255)
                          ggTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTg                                                               <  2:1528990/93‑1 (MQ=255)
                           gTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGc                                                              <  2:2107502/93‑1 (MQ=255)
                            tcatcaCGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGcc                                                             >  1:497296/1‑93 (MQ=255)
                            tcatcaCGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGcc                                                             >  1:3003829/1‑93 (MQ=255)
                            tcatcaCGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGcc                                                             >  1:6302274/1‑93 (MQ=255)
                             catcaCGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCg                                                            >  1:3697300/1‑93 (MQ=255)
                              atcaCGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGc                                                           >  2:4665744/1‑93 (MQ=255)
                              atcaCGGTCACGGAATTCACGTTTCGGACGCATCCGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGTCGTGCCGc                                                           >  2:4931000/1‑93 (MQ=255)
                               tcacggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCGGCa                                                          >  1:7561891/1‑93 (MQ=255)
                               tcacggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCa                                                          >  2:7479841/1‑93 (MQ=255)
                               tcacggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCa                                                          >  2:6805706/1‑93 (MQ=255)
                               tcacggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCa                                                          >  2:4071477/1‑93 (MQ=255)
                                cacggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAg                                                         <  1:1745672/93‑1 (MQ=255)
                                cacggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAg                                                         >  2:1591220/1‑93 (MQ=255)
                                 acggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGc                                                        >  2:7383969/1‑93 (MQ=255)
                                 acggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGc                                                        <  1:6006725/93‑1 (MQ=255)
                                 acggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGc                                                        <  1:7738079/93‑1 (MQ=255)
                                 acggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGc                                                        >  1:2666193/1‑93 (MQ=255)
                                 acggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGc                                                        <  1:5722549/93‑1 (MQ=255)
                                  cggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGcc                                                       >  1:1101862/1‑93 (MQ=255)
                                   ggtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa                                                      <  2:958492/93‑1 (MQ=255)
                                    gtcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCag                                                     >  2:7802392/1‑93 (MQ=255)
                                     tgacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCaga                                                    <  1:1556015/91‑1 (MQ=255)
                                     tcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCaga                                                    <  2:4401156/93‑1 (MQ=255)
                                     tcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCaga                                                    <  1:3477823/93‑1 (MQ=255)
                                     tcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCaga                                                    <  2:6158473/93‑1 (MQ=255)
                                     tcacggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCaga                                                    <  2:904013/93‑1 (MQ=255)
                                        cggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt                                                 <  1:746005/93‑1 (MQ=255)
                                         ggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt                                                <  1:2616665/93‑1 (MQ=255)
                                         ggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCAGCAGCCAGAGttt                                                >  2:3280462/1‑93 (MQ=255)
                                            aTTCACGTTTCGGACGAATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCga                                             <  1:6999006/93‑1 (MQ=255)
                                             ttCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCgag                                            >  1:2512256/1‑93 (MQ=255)
                                              tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCgaga                                           <  1:7853963/93‑1 (MQ=255)
                                                aCGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc                                         <  1:5375094/93‑1 (MQ=255)
                                                aCGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc                                         >  2:472314/1‑93 (MQ=255)
                                                aCGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc                                         >  1:1873924/1‑93 (MQ=255)
                                                aCGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc                                         >  1:6505331/1‑93 (MQ=255)
                                                 cGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATcc                                        <  1:3570614/93‑1 (MQ=255)
                                                  gTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCa                                       <  1:4284694/93‑1 (MQ=255)
                                                  gTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCa                                       >  2:6888672/1‑93 (MQ=255)
                                                   tttCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAg                                      <  2:3444654/93‑1 (MQ=255)
                                                   tttCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAg                                      >  1:2098044/1‑93 (MQ=255)
                                                      cGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGctc                                   <  2:508907/93‑1 (MQ=255)
                                                       ggACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGctct                                  <  1:4543909/93‑1 (MQ=255)
                                                       ggACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGctct                                  <  1:4512687/93‑1 (MQ=255)
                                                        gACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCtt                                 <  1:7400456/93‑1 (MQ=255)
                                                        gACGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCtt                                 >  2:7062471/1‑93 (MQ=255)
                                                          cGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCa                               >  1:1429767/1‑93 (MQ=255)
                                                          cGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCa                               >  2:3549333/1‑93 (MQ=255)
                                                          cGCATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCa                               <  1:3917720/93‑1 (MQ=255)
                                                           ccATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc                              <  2:6962959/92‑1 (MQ=255)
                                                            cATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAcc                             <  1:3404771/93‑1 (MQ=255)
                                                            cATCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAcc                             <  1:5253597/93‑1 (MQ=255)
                                                              tCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt                           >  1:7692664/1‑93 (MQ=255)
                                                              tCGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCACTGCCGCAGCCAGAGATTCGAGATCCAGCTCTTCACCtt                           >  1:214639/1‑93 (MQ=255)
                                                              tCGGCGCATCTGGCGGTACGATCAGAGTGCGGTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt                           <  1:2753769/93‑1 (MQ=255)
                                                               cGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTc                          <  1:3164760/93‑1 (MQ=255)
                                                               cGGCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTc                          >  2:1743550/1‑93 (MQ=255)
                                                                ggCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTca                         <  2:3827365/93‑1 (MQ=255)
                                                                ggCGCATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTca                         <  1:5598855/93‑1 (MQ=255)
                                                                 gcgcATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcag                        >  2:3047487/1‑93 (MQ=255)
                                                                 gcgcATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcag                        >  2:1331038/1‑93 (MQ=255)
                                                                  cgcATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcagc                       <  1:5472571/93‑1 (MQ=255)
                                                                   gcATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcagca                      >  1:6091647/1‑93 (MQ=255)
                                                                   gcATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcagca                      <  2:1282791/93‑1 (MQ=255)
                                                                    cATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcagcag                     <  1:5761547/93‑1 (MQ=255)
                                                                    cATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcagcag                     >  2:3352191/1‑93 (MQ=255)
                                                                    cATCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcagcag                     >  2:6312318/1‑93 (MQ=255)
                                                                     aTCTGGCGGTACGATCAGAGTGCGTTCGCCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGt                    <  2:268857/93‑1 (MQ=255)
                                                                      tCTGGCGGTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTc                   >  2:6620404/1‑93 (MQ=255)
                                                                           cagTACGATCAGAGTGCGTACAACCTGTGCCATTTCCAGCAGGGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTACAGCAGTCGGGTg              <  2:525703/91‑1 (MQ=255)
                                                                             gTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGaa            <  2:546117/93‑1 (MQ=255)
                                                                             gTACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGaa            <  2:5572285/93‑1 (MQ=255)
                                                                              tACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAt           <  2:3690209/93‑1 (MQ=255)
                                                                              tACGATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAt           >  1:4729086/1‑93 (MQ=255)
                                                                                 gATCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtttt        >  2:2291991/1‑93 (MQ=255)
                                                                                  aTCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTg       >  1:7190176/1‑93 (MQ=255)
                                                                                  aTCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTg       <  1:283983/93‑1 (MQ=255)
                                                                                   tCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGc      >  2:1232472/1‑93 (MQ=255)
                                                                                   tCAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGc      >  1:4357168/1‑93 (MQ=255)
                                                                                    cAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCt     <  2:1017967/93‑1 (MQ=255)
                                                                                    cAGAGTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCt     <  2:6570863/93‑1 (MQ=255)
                                                                                     agagTGCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTc    <  2:2016955/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACGGTCGTTACGATCGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAG  >  NC_000913/3304104‑3304283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑