BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,365,636	A→G	R59G (AGG→GGG)	yhcG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,365,636	0	A	G	99.7%	1124.8 / ‑5.6	358	R59G (AGG→GGG)	yhcG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (140/217); total (140/218)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 379 total aligned reads displayed.

GAAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGC  >  NC_000913/3365546‑3365725

gaAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCggg                                                                                         >  2:809955/1‑93 (MQ=255)

gaAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCggg                                                                                         >  1:2283922/1‑93 (MQ=255)

  aaCGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGc                                                                                       <  2:3553037/93‑1 (MQ=255)

  aaCGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGc                                                                                       >  2:555198/1‑93 (MQ=255)

     ggTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGc                                                                                    <  1:6744325/93‑1 (MQ=255)

     ggTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGc                                                                                    <  1:4656439/93‑1 (MQ=255)

     ggTACGTAGAGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGc                                                                                    >  2:5383338/1‑93 (MQ=255)

       tACCTAGCGTTAACGCGTTAATGACCGAGACGTAACAGGAAATTAGACGACGACTTGTAGAATTTGAAAAAGGTGGCGAGGCCGGAGCTGCGt                                                                                  >  2:3868255/1‑93 (MQ=255)

        aCGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTa                                                                                 <  2:1990005/93‑1 (MQ=255)

        aCGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTa                                                                                 >  2:3133480/1‑93 (MQ=255)

          gTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATg                                                                               <  1:650508/93‑1 (MQ=255)

          gTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATg                                                                               >  2:785811/1‑93 (MQ=255)

          gTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATg                                                                               >  1:5075474/1‑93 (MQ=255)

          gTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATg                                                                               >  1:7407186/1‑93 (MQ=255)

           tAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATgg                                                                              >  2:924150/1‑93 (MQ=255)

           gaGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATgg                                                                              <  1:2169958/92‑1 (MQ=255)

            aGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGt                                                                             <  2:3242937/93‑1 (MQ=255)

             gCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTg                                                                            >  1:5993124/1‑93 (MQ=255)

             gCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTg                                                                            <  2:6362618/93‑1 (MQ=255)

             gCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGCATGCGTATGGTg                                                                            >  1:2811845/1‑93 (MQ=255)

             gCGTTAACGCGTTAATGACCGCGACGTACCACGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGACGCCGGGTCTGCGTATGGTg                                                                            >  2:1802499/1‑93 (MQ=255)

                ttAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTgcgc                                                                         <  1:872561/93‑1 (MQ=255)

                  aaCGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAg                                                                       >  1:3904517/1‑93 (MQ=255)

                  aaCGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAg                                                                       >  2:712023/1‑93 (MQ=255)

                   aCGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGc                                                                      <  2:3035828/93‑1 (MQ=255)

                    cgcgTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCt                                                                     <  2:1527043/93‑1 (MQ=255)

                      cgTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTaa                                                                   >  2:6076960/1‑93 (MQ=255)

                       gTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAAt                                                                  >  2:7579128/1‑93 (MQ=255)

                       gTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAAt                                                                  <  1:3215276/93‑1 (MQ=255)

                        ttAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATc                                                                 <  1:7547360/93‑1 (MQ=255)

                        ttAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATc                                                                 <  2:7248343/93‑1 (MQ=255)

                         tAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCa                                                                >  1:7306482/1‑93 (MQ=255)

                           aTGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAg                                                              <  1:5894751/93‑1 (MQ=255)

                            tGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGc                                                             <  2:5140429/93‑1 (MQ=255)

                            tGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGc                                                             <  1:5259008/93‑1 (MQ=255)

                               ccGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGAc                                                          <  1:7469976/93‑1 (MQ=255)

                               ccGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGAc                                                          <  1:6252528/93‑1 (MQ=255)

                                cgcgACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACt                                                         >  1:4376910/1‑93 (MQ=255)

                                  cgACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTAt                                                       >  2:3694911/1‑93 (MQ=255)

                                   gACGTACCAGGAAATGGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATc                                                      <  2:5289662/93‑1 (MQ=255)

                                    aCGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCa                                                     >  2:1374436/1‑93 (MQ=255)

                                    aCGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCa                                                     >  2:4001911/1‑93 (MQ=255)

                                      gTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGGGACTATCaaa                                                   >  2:165438/1‑93 (MQ=255)

                                      gTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCaaa                                                   <  1:4155017/93‑1 (MQ=255)

                                       tACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAg                                                  >  1:1222584/1‑93 (MQ=255)

                                       tACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAg                                                  >  2:250287/1‑93 (MQ=255)

                                       tACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAg                                                  <  1:3050126/93‑1 (MQ=255)

                                       tACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAg                                                  >  1:2871586/1‑93 (MQ=255)

                                       tACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAg                                                  >  2:5504967/1‑93 (MQ=255)

                                       tACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAg                                                  <  1:69354/93‑1 (MQ=255)

                                        aCCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAgg                                                 <  1:4842796/93‑1 (MQ=255)

                                          cAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTTATCAAGCGACTATCAAAGGAt                                               >  2:3727148/1‑93 (MQ=255)

                                          cAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAt                                               <  2:50729/93‑1 (MQ=255)

                                          cAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAt                                               <  1:4754564/93‑1 (MQ=255)

                                           aGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAtt                                              >  1:167869/1‑93 (MQ=255)

                                             gAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTa                                            <  2:5997652/93‑1 (MQ=255)

                                             gAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTa                                            <  2:2114706/93‑1 (MQ=255)

                                               aaTTGGCCGGCGAATTGGCGAATTTGAACAAGGGGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATg                                          <  2:6838460/93‑1 (MQ=255)

                                               aaTTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATg                                          >  1:1012007/1‑93 (MQ=255)

                                                   ggCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTa                                      <  1:2524983/93‑1 (MQ=255)

                                                   ggCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTa                                      <  1:7809917/93‑1 (MQ=255)

                                                   ggCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTa                                      <  2:1776462/93‑1 (MQ=255)

                                                    gCCGAGGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTaa                                     <  2:1270209/93‑1 (MQ=255)

                                                    gCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGGGTATGGTGCGCAGCTAATCAAGCGACTATCAAGGGATTTATGTCTag                                     >  2:2745463/1‑92 (MQ=255)

                                                    gCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTaa                                     <  1:5291397/93‑1 (MQ=255)

                                                    gCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTaa                                     <  1:5377189/93‑1 (MQ=255)

                                                     ccGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAg                                    <  2:6267114/93‑1 (MQ=255)

                                                     ccGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAg                                    <  2:4882668/93‑1 (MQ=255)

                                                     ccGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAg                                    <  1:7920933/93‑1 (MQ=255)

                                                      cgacgaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAgg                                   <  1:6113484/93‑1 (MQ=255)

                                                       gacgaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGt                                  >  2:2623529/1‑93 (MQ=255)

                                                       gacgaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGt                                  >  2:2436729/1‑93 (MQ=255)

                                                        acgaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGta                                 >  2:458649/1‑93 (MQ=255)

                                                        acgaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGta                                 <  2:4699217/93‑1 (MQ=255)

                                                        acgaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGta                                 >  1:448528/1‑93 (MQ=255)

                                                          gaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGtata                               <  2:6709946/93‑1 (MQ=255)

                                                          gaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGtata                               >  1:1799403/1‑93 (MQ=255)

                                                          gaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGtata                               <  2:3427731/93‑1 (MQ=255)

                                                          gaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGtata                               <  1:3463363/93‑1 (MQ=255)

                                                           aaTTGTCGAATGTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATaa                              <  2:2239773/93‑1 (MQ=255)

                                                               gTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGt                          >  2:4722064/1‑93 (MQ=255)

                                                                  gAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTggg                       <  1:2386145/93‑1 (MQ=255)

                                                                  gAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTggg                       >  1:3750298/1‑93 (MQ=255)

                                                                    aTTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGtt                     >  2:393994/1‑93 (MQ=255)

                                                                      ttGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtct                   <  2:4138964/93‑1 (MQ=255)

                                                                       tGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtctc                  >  2:1848637/1‑93 (MQ=255)

                                                                       tGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtctc                  <  1:1363420/93‑1 (MQ=255)

                                                                       tGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtctc                  >  1:1633766/1‑93 (MQ=255)

                                                                       tGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtctc                  <  2:445980/93‑1 (MQ=255)

                                                                       tGAACAAGGTGGCAAGGCCGGGGCTGCGTATGGTGCACAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtctc                  <  1:2708287/93‑1 (MQ=255)

                                                                         aaCAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTg                <  1:4200030/93‑1 (MQ=255)

                                                                             aGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCaaa            >  1:6855860/1‑93 (MQ=255)

                                                                               gTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCaaaaa          <  1:7030307/93‑1 (MQ=255)

                                                                                tGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCaaaaaa         <  1:6357146/93‑1 (MQ=255)

                                                                                tGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCaaaaaa         <  1:1587795/93‑1 (MQ=255)

                                                                                    gAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTa     <  2:690280/93‑1 (MQ=255)

                                                                                     aGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTAc    >  2:5744834/1‑93 (MQ=255)

                                                                                     aGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTAc    <  2:4243147/93‑1 (MQ=255)

                                                                                     aGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTAc    >  2:7725177/1‑93 (MQ=255)

                                                                                       gCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGc  <  2:7365129/93‑1 (MQ=255)

GAAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGC  >  NC_000913/3365546‑3365725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`