BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,054	A→G	H198R (CAC→CGC)	yhcG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,054	0	A	G	99.7%	970.2 / NA	312	H198R (CAC→CGC)	yhcG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (143/168); total (143/169)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 336 total aligned reads displayed.

GTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACACGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAG  >  NC_000913/3365965‑3366143

gTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACgcgc                                                                                        >  1:3893756/1‑93 (MQ=255)

 tCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCt                                                                                       >  1:1409889/1‑93 (MQ=255)

  cTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTc                                                                                      >  1:3646307/1‑93 (MQ=255)

    gTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCt                                                                                    >  2:2510711/1‑93 (MQ=255)

    gTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCt                                                                                    >  1:6450904/1‑93 (MQ=255)

     ttCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTg                                                                                   <  2:3688750/93‑1 (MQ=255)

      tCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGc                                                                                  <  1:1306644/93‑1 (MQ=255)

       cGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGcc                                                                                 <  2:6088339/93‑1 (MQ=255)

       cGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGcc                                                                                 <  1:328701/93‑1 (MQ=255)

       cGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGcc                                                                                 >  2:7581821/1‑93 (MQ=255)

        gCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCg                                                                                <  2:4370084/93‑1 (MQ=255)

         ccAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCga                                                                               >  1:6332611/1‑93 (MQ=255)

          cAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCgag                                                                              >  2:7249218/1‑93 (MQ=255)

          cAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCgag                                                                              >  1:1211179/1‑93 (MQ=255)

            gCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGAc                                                                            <  1:477850/93‑1 (MQ=255)

             cTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACg                                                                           >  1:7533644/1‑93 (MQ=255)

              tAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGc                                                                          >  1:4830665/1‑93 (MQ=255)

               agagCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCa                                                                         >  2:812131/1‑93 (MQ=255)

                 agCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCata                                                                       <  2:5670134/93‑1 (MQ=255)

                 agCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCata                                                                       <  2:1305182/93‑1 (MQ=255)

                  gCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCatat                                                                      >  1:2024130/1‑93 (MQ=255)

                  gCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCatat                                                                      >  2:5445501/1‑93 (MQ=255)

                    ggCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAttt                                                                    >  1:1425983/1‑92 (MQ=255)

                    ggCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCCCCTGCCGAGACGCATAttc                                                                    >  1:2692151/1‑93 (MQ=255)

                       aaaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAttcttc                                                                 >  1:5218566/1‑93 (MQ=255)

                       aaaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAttcttc                                                                 <  1:6646599/93‑1 (MQ=255)

                       aaaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAttcttc                                                                 <  2:921651/93‑1 (MQ=255)

                        aaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTcc                                                                <  2:6826859/93‑1 (MQ=255)

                        aaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTcc                                                                <  1:6262333/93‑1 (MQ=255)

                          ttGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGc                                                              >  1:6732834/1‑93 (MQ=255)

                              gACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACa                                                          >  1:303005/1‑93 (MQ=255)

                              gACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACa                                                          <  1:2526848/93‑1 (MQ=255)

                              gACCAAGTTTTAGGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACa                                                          <  1:7656377/93‑1 (MQ=255)

                                 ccAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGc                                                       <  1:7433352/93‑1 (MQ=255)

                                  cAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGACACGCATATTCTTCCGCAACAGGCg                                                      >  2:6677461/1‑93 (MQ=255)

                                  cAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCGGCCGAGACGCATATCCTTCCGCAACAgcga                                                      >  1:1626151/1‑90 (MQ=255)

                                    tttttATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCCTCCGCAACAGGCGAt                                                    >  2:7033728/2‑93 (MQ=255)

                                    gTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGAt                                                    >  1:2247245/1‑93 (MQ=255)

                                    gTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGAt                                                    >  1:3082824/1‑93 (MQ=255)

                                    gTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGAt                                                    <  2:7859112/93‑1 (MQ=255)

                                     ttttATGAGCGGACACTACTGTCAGATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATa                                                   <  2:4298121/93‑1 (MQ=255)

                                     ttttATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATa                                                   >  1:7112219/1‑93 (MQ=255)

                                     ttttATGAGCGGACACTACTGTCAAATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATa                                                   <  1:259903/93‑1 (MQ=255)

                                          tGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGa                                              >  1:4147166/1‑93 (MQ=255)

                                           gAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGAt                                             <  2:2091347/93‑1 (MQ=255)

                                             gCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATcc                                           <  2:2201693/93‑1 (MQ=255)

                                             gCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATcc                                           >  1:1786445/1‑93 (MQ=255)

                                              cGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATccc                                          <  1:6908265/93‑1 (MQ=255)

                                               ggACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCt                                         <  2:5508747/93‑1 (MQ=255)

                                                gACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCtt                                        <  1:3121990/93‑1 (MQ=255)

                                                 acacTACTGTCACATGACAAGTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCttt                                       >  2:7613440/1‑93 (MQ=255)

                                                 acacTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCttt                                       <  2:5852557/93‑1 (MQ=255)

                                                  cacTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtg                                      <  1:4933666/93‑1 (MQ=255)

                                                   actactGTCACATGACAACTCAGCCCTGCTGCAACCACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtgt                                     >  1:6951696/1‑93 (MQ=255)

                                                    ctactGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtgtg                                    >  1:2409497/1‑93 (MQ=255)

                                                    ctactGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtgtg                                    >  2:4645249/1‑93 (MQ=255)

                                                     tactGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGc                                   >  2:766105/1‑93 (MQ=255)

                                                      actGTCACATGACAAATCAGCCATGCTGTAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCt                                  <  2:6020527/93‑1 (MQ=255)

                                                      actGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCt                                  >  1:4461761/1‑93 (MQ=255)

                                                      actGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGAAACAGGCGATACGCGATCCCTTTGTGCt                                  >  2:4021691/1‑93 (MQ=255)

                                                       ctGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTc                                 <  1:931277/93‑1 (MQ=255)

                                                         gTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGa                               <  2:189005/93‑1 (MQ=255)

                                                          tCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGaa                              <  2:2963192/93‑1 (MQ=255)

                                                           cacaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAt                             <  2:2770698/93‑1 (MQ=255)

                                                           cacaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAt                             <  1:5614994/93‑1 (MQ=255)

                                                            acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            >  1:608768/1‑93 (MQ=255)

                                                            acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            >  1:4527299/1‑93 (MQ=255)

                                                            acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            >  2:6371153/1‑93 (MQ=255)

                                                             caTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAttt                           >  1:3750034/1‑93 (MQ=255)

                                                              aTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTc                          >  2:1635141/1‑93 (MQ=255)

                                                                gACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTg                        <  1:5010204/93‑1 (MQ=255)

                                                                 aCAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTgg                       <  2:3788777/93‑1 (MQ=255)

                                                                  cAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGa                      <  1:1091313/93‑1 (MQ=255)

                                                                  cAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGa                      <  1:1001510/93‑1 (MQ=255)

                                                                  cAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGa                      >  1:7825838/1‑93 (MQ=255)

                                                                  cAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGa                      >  2:1089465/1‑93 (MQ=255)

                                                                   aaaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGat                     >  2:864182/1‑92 (MQ=255)

                                                                   aaaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGaa                     <  1:3537208/93‑1 (MQ=255)

                                                                   aaaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGaa                     <  2:3151571/93‑1 (MQ=255)

                                                                     aTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAAtt                   >  1:4585829/1‑93 (MQ=255)

                                                                        aGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaa                >  2:5257685/1‑93 (MQ=255)

                                                                         gCCATGCTGCAACAACGCGTTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaaa               <  1:344362/93‑1 (MQ=255)

                                                                         gCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaaa               >  2:3396035/1‑93 (MQ=255)

                                                                         gCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaaa               <  2:722404/93‑1 (MQ=255)

                                                                          ccATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAg              >  2:472602/1‑93 (MQ=255)

                                                                          ccATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAg              <  2:6955309/93‑1 (MQ=255)

                                                                            aTGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGAt            >  1:7695976/1‑93 (MQ=255)

                                                                             tgctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATg           >  2:1739733/1‑93 (MQ=255)

                                                                             tgctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATg           >  1:3824481/1‑93 (MQ=255)

                                                                              gctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGa          <  2:4312923/93‑1 (MQ=255)

                                                                              gctgcAACAACACGCTCCTGCCGAGTCGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGa          <  1:5429390/93‑1 (MQ=255)

                                                                               ctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGaa         <  2:5154656/93‑1 (MQ=255)

                                                                                tgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAat        >  2:3257962/1‑93 (MQ=255)

                                                                                tgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAat        <  2:7779928/93‑1 (MQ=255)

                                                                                 gcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAata       >  2:5040299/1‑93 (MQ=255)

                                                                                 gcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAata       <  1:4659643/93‑1 (MQ=255)

                                                                                   aacaacGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATAtt     <  1:5377478/93‑1 (MQ=255)

                                                                                    acaacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTc    >  1:587811/1‑93 (MQ=255)

                                                                                     caacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCa   <  1:7002576/93‑1 (MQ=255)

                                                                                      aacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAg  >  2:6514659/1‑93 (MQ=255)

GTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACACGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAG  >  NC_000913/3365965‑3366143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`