BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	374,060	G→A	E323E (GAG→GAA)	mhpE →	4‑hydroxy‑2‑ketovalerate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	374,060	0	G	A	100.0%	1123.2 / NA	309	E323E (GAG→GAA)	mhpE	4‑hydroxy‑2‑ketovalerate aldolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (155/154); total (155/154)

Only 100 of 328 total aligned reads displayed.

TCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAC  >  NC_000913/373970‑374149

tCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAggaaga                                                                                         >  1:4062378/1‑93 (MQ=255)

  aCTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGata                                                                                       >  1:2285336/1‑93 (MQ=255)

   cTGTGAAACGGCGGTGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGatat                                                                                      <  2:666036/93‑1 (MQ=255)

   cTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGatat                                                                                      <  1:2706740/93‑1 (MQ=255)

    tgtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATg                                                                                     >  1:3395714/1‑93 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGa                                                                                    <  1:626757/93‑1 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGa                                                                                    <  2:455497/93‑1 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGa                                                                                    <  1:4246965/93‑1 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGa                                                                                    <  2:2786531/93‑1 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGa                                                                                    <  1:101551/93‑1 (MQ=255)

       gAAACGGCGGCGGCTCGTTATGGCTTAAGTGCGGTTGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATc                                                                                  <  1:1824437/93‑1 (MQ=255)

       gAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATc                                                                                  >  2:4672629/1‑93 (MQ=255)

         aaCGGCGGCGGGGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGt                                                                                <  1:3635615/93‑1 (MQ=255)

          aCGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGtt                                                                               <  1:4177684/93‑1 (MQ=255)

          aCGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGtt                                                                               >  2:2713546/1‑93 (MQ=255)

            ggcggcggcGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGa                                                                             <  1:5754072/93‑1 (MQ=255)

            ggcggcggcGCGTTATGGCTTAAGTGCGGTGGATATTCTCGCTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGa                                                                             >  1:6922075/1‑93 (MQ=255)

             gcggcggcgCGTTATGGGTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGAc                                                                            <  1:3088772/93‑1 (MQ=255)

             gcggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGAc                                                                            <  2:2244379/93‑1 (MQ=255)

             gcggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGAc                                                                            >  2:4110185/1‑93 (MQ=255)

               ggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGt                                                                          <  1:6205097/93‑1 (MQ=255)

               ggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGt                                                                          <  2:2446004/93‑1 (MQ=255)

               ggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGt                                                                          >  1:3540649/1‑93 (MQ=255)

               ggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGt                                                                          <  1:1107164/93‑1 (MQ=255)

                  ggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgc                                                                       >  2:3752062/1‑93 (MQ=255)

                   gcgcGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcg                                                                      <  2:3506274/93‑1 (MQ=255)

                   gcgcGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcg                                                                      >  2:7329016/1‑93 (MQ=255)

                    cgcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcgc                                                                     <  1:6768591/93‑1 (MQ=255)

                     gcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCt                                                                    >  2:6235625/1‑93 (MQ=255)

                     gcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCt                                                                    >  2:7687632/1‑93 (MQ=255)

                     gcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCt                                                                    >  1:6018050/1‑93 (MQ=255)

                      cgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTg                                                                   <  1:5166942/93‑1 (MQ=255)

                       gTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTgg                                                                  >  2:1803762/1‑93 (MQ=255)

                       gTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTgg                                                                  >  1:7892214/1‑93 (MQ=255)

                        ttATGGCTTAAGTGCGGGGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGTAGATATGATCGGTGACGTGGCGCTGGa                                                                 >  2:7024941/1‑93 (MQ=255)

                         tATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                >  1:7557738/1‑93 (MQ=255)

                         tATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                <  2:301875/93‑1 (MQ=255)

                         tATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                >  1:4622785/1‑93 (MQ=255)

                         tATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                >  2:2070605/1‑93 (MQ=255)

                         tATGGCTTAAGTGCGGGGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGGTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                <  1:1083315/93‑1 (MQ=255)

                           tGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGTAGATATGATCGTTGACGTCGCGCTGGATCt                                                              >  1:2457014/1‑93 (MQ=255)

                           tGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCt                                                              >  2:640985/1‑93 (MQ=255)

                           tGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCt                                                              >  1:222076/1‑93 (MQ=255)

                           tGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATAGTTGACGTGGCGCTGGATCt                                                              >  2:3949263/1‑93 (MQ=255)

                            ggCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGGGCTGGATCTg                                                             >  1:689106/1‑93 (MQ=255)

                            ggCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTg                                                             <  1:4698240/93‑1 (MQ=255)

                             gCTTAAGTGCGGGGGATATTCTCGTTGAGCTGGGCAAACGTCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCGGGATCTgc                                                            <  2:1365127/93‑1 (MQ=255)

                                  aGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaac                                                       <  1:6464856/93‑1 (MQ=255)

                                      cGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaacaaca                                                   <  2:3223627/93‑1 (MQ=255)

                                       ggTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaacaacaa                                                  <  1:5049722/93‑1 (MQ=255)

                                       ggTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaacaacaa                                                  <  2:5839707/93‑1 (MQ=255)

                                         tGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaat                                                >  1:5010525/1‑93 (MQ=255)

                                          ggATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaata                                               <  1:5329000/93‑1 (MQ=255)

                                           gATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCATGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaataa                                              <  1:1696244/93‑1 (MQ=255)

                                           gATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaacaac                                                    <  1:910779/87‑1 (MQ=255)

                                           gATATGCTCGTTGAGCTGGCCAAACGCAGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaataa                                              <  1:2893558/93‑1 (MQ=255)

                                            atatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaataat                                             <  2:715236/93‑1 (MQ=255)

                                            atatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaataat                                             <  2:5381957/93‑1 (MQ=255)

                                            atatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaataat                                             <  2:5620549/93‑1 (MQ=255)

                                             tatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatg                                            >  2:7431522/1‑93 (MQ=255)

                                             tatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatg                                            >  2:5066994/1‑93 (MQ=255)

                                              atgctcGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           <  1:417556/90‑1 (MQ=255)

                                              atTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           >  2:6822139/1‑93 (MQ=255)

                                              atTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           >  1:7232142/1‑93 (MQ=255)

                                               ttCTTGTTGAGCTGGGCAAACGCCGGTTGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatgat                                          <  1:4663715/93‑1 (MQ=255)

                                               ttCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatgat                                          <  1:1299872/93‑1 (MQ=255)

                                               ttCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatgat                                          >  2:3348960/1‑93 (MQ=255)

                                               ttCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatgat                                          >  1:6334262/1‑93 (MQ=255)

                                                 ctcGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAAtgatga                                        <  2:4945384/93‑1 (MQ=255)

                                                 ctcGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAAtgatga                                        <  2:3587385/93‑1 (MQ=255)

                                                 ctcGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAAtgatga                                        <  2:115070/93‑1 (MQ=255)

                                                  tcGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGAc                                       >  1:2588575/1‑93 (MQ=255)

                                                       gAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCg                                  >  2:5925196/1‑93 (MQ=255)

                                                       gAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCg                                  >  2:1428768/1‑93 (MQ=255)

                                                           tGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGaa                              <  2:5519994/93‑1 (MQ=255)

                                                           tGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGaa                              <  2:1582955/93‑1 (MQ=255)

                                                               cAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTg                          <  2:5739289/93‑1 (MQ=255)

                                                                 aaCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCa                        >  2:6917423/1‑93 (MQ=255)

                                                                  aCGCGGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAt                       <  1:3351285/93‑1 (MQ=255)

                                                                  aCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAt                       <  2:8132/93‑1 (MQ=255)

                                                                    gCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAttt                     >  2:6744481/1‑93 (MQ=255)

                                                                     ccGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAtttt                    <  2:4278099/93‑1 (MQ=255)

                                                                     ccGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAtttt                    <  2:5109955/93‑1 (MQ=255)

                                                                      cGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTg                   >  2:4190900/1‑93 (MQ=255)

                                                                       ggATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGt                  >  1:1956324/1‑93 (MQ=255)

                                                                        gATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTa                 <  1:4477453/93‑1 (MQ=255)

                                                                         aTGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTAt                >  1:7685807/1‑93 (MQ=255)

                                                                         aTGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTAt                <  1:968841/93‑1 (MQ=255)

                                                                         aTGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTAt                >  2:1972257/1‑93 (MQ=255)

                                                                         aTGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTAt                <  2:1217167/93‑1 (MQ=255)

                                                                             gtGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGccc            <  1:1157427/92‑1 (MQ=255)

                                                                              tGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCa           <  2:5455710/93‑1 (MQ=255)

                                                                               ggcggcCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCAt          >  2:6109990/1‑93 (MQ=255)

                                                                                gcggcCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATc         >  2:6134518/1‑93 (MQ=255)

                                                                                   gcCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCa      <  1:4859071/93‑1 (MQ=255)

                                                                                      aggaagATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGcc   >  1:3723339/1‑92 (MQ=255)

                                                                                      aggaagATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCa   >  1:7283908/1‑93 (MQ=255)

                                                                                       ggaagaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAc  >  1:7416804/1‑93 (MQ=255)

                                                                                       ggaagaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAc  >  1:7826059/1‑93 (MQ=255)

                                                                                       ggaagaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAc  >  1:1544067/1‑93 (MQ=255)

TCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAC  >  NC_000913/373970‑374149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`