BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,407,232	G→A	A176T (GCG→ACG)	prmA →	PrmA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,407,232	0	G	A	99.7%	1254.2 / NA	354	A176T (GCG→ACG)	prmA	PrmA
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (160/193); minor base C (0/1); total (160/194)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 378 total aligned reads displayed.

CTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGC  >  NC_000913/3407141‑3407321

 tctctGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCgcgacg                                                                                         <  1:7431217/93‑1 (MQ=255)

 tctctGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTGCCGGCATTCTGGCGATCgcgacg                                                                                         >  2:4291705/1‑93 (MQ=255)

      gtgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGaa                                                                                    >  2:5586857/1‑93 (MQ=255)

      gtgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGaa                                                                                    >  2:1608938/1‑93 (MQ=255)

       tgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGaaa                                                                                   <  2:717886/93‑1 (MQ=255)

        gCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAAc                                                                                  >  1:2747103/1‑93 (MQ=255)

        gCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAAc                                                                                  >  1:7743203/1‑93 (MQ=255)

          cTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTg                                                                                <  1:4631056/93‑1 (MQ=255)

           tGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCCGGCGATCGCGACGCTGAAACTgg                                                                               >  2:1109178/1‑93 (MQ=255)

            gCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTggg                                                                              <  2:3550063/93‑1 (MQ=255)

            gCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTggg                                                                              <  1:5997912/93‑1 (MQ=255)

              aaTGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTg                                                                            >  1:5955058/1‑93 (MQ=255)

               aTGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTgc                                                                           >  2:1293089/1‑93 (MQ=255)

               aTGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTgc                                                                           <  1:3224917/93‑1 (MQ=255)

                tGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTgca                                                                          <  1:1907565/93‑1 (MQ=255)

                      gACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCaaaa                                                                    <  1:6286300/93‑1 (MQ=255)

                        cAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGc                                                                  >  1:1336433/1‑93 (MQ=255)

                         aGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGcc                                                                 <  1:3752842/93‑1 (MQ=255)

                          gcgtCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCa                                                                <  2:4753292/90‑1 (MQ=255)

                          gCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCa                                                                <  2:7491197/93‑1 (MQ=255)

                           ccTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGTCAAAGCCAt                                                               >  1:113400/1‑93 (MQ=255)

                           ccTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCAt                                                               >  1:5299859/1‑93 (MQ=255)

                               gATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGt                                                           >  2:5147124/1‑93 (MQ=255)

                               gATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGt                                                           >  2:5977942/1‑93 (MQ=255)

                                 tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         >  1:3867749/1‑93 (MQ=255)

                                 tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         >  2:2749488/1‑93 (MQ=255)

                                 tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         >  1:5405795/1‑93 (MQ=255)

                                  ttAACCGGTAAAACAGTCATCGACTCTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAtt                                                        <  1:4168027/93‑1 (MQ=255)

                                    aaCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGa                                                      <  1:1997029/93‑1 (MQ=255)

                                    aaCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGa                                                      <  2:2032027/93‑1 (MQ=255)

                                    aaCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGa                                                      >  2:6540831/1‑93 (MQ=255)

                                     aCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGat                                                     <  1:3420533/93‑1 (MQ=255)

                                      ccGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGata                                                    <  2:226618/93‑1 (MQ=255)

                                      ccGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGATGCAGCAAAAGCCATTGGTATTGata                                                    <  1:1465006/93‑1 (MQ=255)

                                      ccGCTAAAACAGTCATCGTCTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGata                                                    <  2:1718922/93‑1 (MQ=255)

                                       cGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGatat                                                   <  2:3878959/93‑1 (MQ=255)

                                       cGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGatat                                                   <  1:1055421/93‑1 (MQ=255)

                                        ggTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATc                                                  <  1:3056082/93‑1 (MQ=255)

                                        ggTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATc                                                  <  2:2183733/93‑1 (MQ=255)

                                          tAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGa                                                <  2:7927237/93‑1 (MQ=255)

                                           aaaaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGAt                                               <  1:5513406/93‑1 (MQ=255)

                                           aaaaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGAt                                               <  2:6458431/93‑1 (MQ=255)

                                           aaaaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGAt                                               <  2:6804398/93‑1 (MQ=255)

                                             aaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATcc                                             >  1:2238407/1‑93 (MQ=255)

                                             aaCAGTCATCGACGTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACTCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATcc                                             <  1:5849200/93‑1 (MQ=255)

                                              aCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCg                                            >  1:6919133/1‑93 (MQ=255)

                                               cAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGc                                           >  1:7852817/1‑93 (MQ=255)

                                               cAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGc                                           >  2:4195394/1‑93 (MQ=255)

                                                aatCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCa                                          <  2:4058719/91‑1 (MQ=255)

                                                aGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCa                                          <  1:2343596/93‑1 (MQ=255)

                                                aGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCa                                          <  1:1716135/93‑1 (MQ=255)

                                                aGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCa                                          <  2:5243834/93‑1 (MQ=255)

                                                aGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCa                                          <  1:5793141/93‑1 (MQ=255)

                                                 gTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAg                                         >  2:3439578/1‑93 (MQ=255)

                                                 gTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAg                                         >  2:5515508/1‑93 (MQ=255)

                                                  tCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAgg                                        >  1:7620985/1‑93 (MQ=255)

                                                   cATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGc                                       <  1:3517945/93‑1 (MQ=255)

                                                   cATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGc                                       <  1:840744/93‑1 (MQ=255)

                                                   cATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGc                                       >  2:7726217/1‑93 (MQ=255)

                                                    aTCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCg                                      >  2:1146874/1‑93 (MQ=255)

                                                     tCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa                                     <  1:3588587/93‑1 (MQ=255)

                                                     tCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa                                     >  1:6813259/1‑93 (MQ=255)

                                                         cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 <  1:3313532/93‑1 (MQ=255)

                                                         cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 <  2:2792536/93‑1 (MQ=255)

                                                         cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 >  1:7506820/1‑93 (MQ=255)

                                                         cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 >  1:4078718/1‑93 (MQ=255)

                                                         cTTTGGCTGTGGTTCCGGCATTCGGGCGATCGCGCCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 <  1:1845411/93‑1 (MQ=255)

                                                          tttGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAg                                <  2:6252482/93‑1 (MQ=255)

                                                          tttGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAg                                >  1:7236254/1‑93 (MQ=255)

                                                          tttGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAg                                >  1:916241/1‑93 (MQ=255)

                                                            tGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGc                              <  1:6402667/93‑1 (MQ=255)

                                                             ggCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGcc                             <  2:6302371/93‑1 (MQ=255)

                                                             ggCTGTGGTTCCGGAATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGcc                             <  1:4327754/93‑1 (MQ=255)

                                                               cTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAg                           <  2:2365493/93‑1 (MQ=255)

                                                                tgtgGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGc                          >  1:4704932/1‑93 (MQ=255)

                                                                tgtgGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGc                          <  2:6091455/93‑1 (MQ=255)

                                                                      tCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt                    >  2:4894673/1‑93 (MQ=255)

                                                                        cGGCATTCTGTCGATCGCGACGCTGAACCTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa                  <  1:4917008/93‑1 (MQ=255)

                                                                        cGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa                  <  1:2933676/93‑1 (MQ=255)

                                                                        cGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa                  >  2:3279198/1‑93 (MQ=255)

                                                                         ggCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAAc                 <  2:3113197/93‑1 (MQ=255)

                                                                          gCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACg                >  2:893053/1‑93 (MQ=255)

                                                                          gCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACg                <  2:5679038/93‑1 (MQ=255)

                                                                           cATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGc               >  2:5007046/1‑93 (MQ=255)

                                                                           cATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGc               <  2:1214954/93‑1 (MQ=255)

                                                                            aTTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGcc              >  1:1592262/1‑93 (MQ=255)

                                                                            aTTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGcc              >  2:370415/1‑93 (MQ=255)

                                                                             ttCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCGGCCGCGATAACGCCg             >  2:4532613/1‑93 (MQ=255)

                                                                             ttCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg             <  2:3037508/93‑1 (MQ=255)

                                                                             ttCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg             >  2:4680633/1‑93 (MQ=255)

                                                                               cTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGaa           <  2:3806034/93‑1 (MQ=255)

                                                                                tGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAAc          <  2:4410455/93‑1 (MQ=255)

                                                                                  gCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt        >  2:194062/1‑93 (MQ=255)

                                                                                  gCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt        <  2:3478655/93‑1 (MQ=255)

                                                                                   cGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTa       <  1:656173/93‑1 (MQ=255)

                                                                                    gATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTaa      <  1:2544192/93‑1 (MQ=255)

                                                                                     aTCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAAt     <  2:7360084/93‑1 (MQ=255)

                                                                                     aTCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAAt     >  1:2668423/1‑93 (MQ=255)

                                                                                     aTCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAAt     >  2:6672939/1‑93 (MQ=255)

                                                                                     aTCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAAt     <  1:1758189/93‑1 (MQ=255)

CTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGC  >  NC_000913/3407141‑3407321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`