BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,746	G→A	A693T (GCC→ACC)	acrF →	AcrF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,746	0	G	A	99.0%	1053.9 / NA	313	A693T (GCC→ACC)	acrF	AcrF
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (151/158); minor base C (0/3); total (151/162)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 327 total aligned reads displayed.

ATTCCATTCAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGAC  >  NC_000913/3414656‑3414835

attccattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATAcc                                                                                         >  2:4450076/1‑93 (MQ=255)

attccattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCAACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATAcc                                                                                         <  2:6807591/93‑1 (MQ=255)

 ttccattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATAccc                                                                                        <  1:4157135/93‑1 (MQ=255)

 ttccattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATAccc                                                                                        >  2:195014/1‑93 (MQ=255)

    cattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTaa                                                                                     >  1:1030614/1‑93 (MQ=255)

     attcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAAc                                                                                    >  1:920825/1‑93 (MQ=255)

     attcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAAc                                                                                    >  2:496718/1‑93 (MQ=255)

      ttcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAAcc                                                                                   >  2:2192832/1‑93 (MQ=255)

       tcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAAccc                                                                                  <  1:1570597/93‑1 (MQ=255)

       tcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAAccc                                                                                  <  2:5608650/93‑1 (MQ=255)

       tcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTCCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAAccc                                                                                  >  2:4472533/1‑93 (MQ=255)

        cAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTTGGCTGGGTCACGATACCCTAACCCa                                                                                 >  2:3580500/1‑93 (MQ=255)

        cAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCa                                                                                 <  2:6694827/93‑1 (MQ=255)

        cAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCa                                                                                 <  1:7545431/93‑1 (MQ=255)

         aaTATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAg                                                                                <  1:1082757/93‑1 (MQ=255)

         aaTATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAg                                                                                >  2:6972844/1‑93 (MQ=255)

         aaTATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAg                                                                                >  1:3242811/1‑93 (MQ=255)

          atatGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAgg                                                                               >  2:1190387/1‑93 (MQ=255)

          atatGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAgg                                                                               >  1:2010341/1‑93 (MQ=255)

           tatGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGc                                                                              >  2:3811037/1‑93 (MQ=255)

            atGCCAGCCATTGTTGAACTGGGCACGGTAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGGCACGATACCCTAACCCAGGcc                                                                             >  2:3016636/1‑93 (MQ=255)

             tGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGccc                                                                            <  1:3394303/93‑1 (MQ=255)

             tGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGccc                                                                            >  1:4049503/1‑93 (MQ=255)

             tGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGccc                                                                            >  2:3983887/1‑93 (MQ=255)

              gccagccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCg                                                                           <  1:2491060/93‑1 (MQ=255)

               ccagccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGt                                                                          <  2:4168488/93‑1 (MQ=255)

                cagccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTa                                                                         >  2:7736911/1‑93 (MQ=255)

                  gccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAAc                                                                       <  1:2854418/93‑1 (MQ=255)

                   ccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAAcc                                                                      >  2:2301070/1‑93 (MQ=255)

                    caTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCa                                                                     <  1:2304399/93‑1 (MQ=255)

                      ttgttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGt                                                                   >  1:4652585/1‑93 (MQ=255)

                      ttgttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGt                                                                   <  2:1424332/93‑1 (MQ=255)

                      ttgttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGt                                                                   >  1:4413228/1‑93 (MQ=255)

                      ttgttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGt                                                                   >  1:1152747/1‑93 (MQ=255)

                        gttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTg                                                                 >  1:1918502/1‑93 (MQ=255)

                          tgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCt                                                               <  1:676577/93‑1 (MQ=255)

                           gAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCtt                                                              <  1:4445551/93‑1 (MQ=255)

                            aaCTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTg                                                             >  2:6054314/1‑93 (MQ=255)

                             aCTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTgg                                                            <  1:4127036/93‑1 (MQ=255)

                             aCTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTgg                                                            <  1:3549815/93‑1 (MQ=255)

                               tGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTa                                                          <  2:706594/93‑1 (MQ=255)

                               tGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTa                                                          <  1:6197789/93‑1 (MQ=255)

                               tGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTa                                                          <  2:3095499/93‑1 (MQ=255)

                               tGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTa                                                          <  2:2550512/93‑1 (MQ=255)

                               tGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTAACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTa                                                          >  2:842477/1‑93 (MQ=255)

                               tGGGCACGGCAACGGGTTTCGACGTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTa                                                          <  2:4303880/93‑1 (MQ=255)

                                gggCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGCTACCCTTACCCAGGCCCGTAACCAGTTGCTTGGGAt                                                         >  2:6413133/1‑93 (MQ=37)

                                gggCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTAt                                                         <  1:3671917/93‑1 (MQ=255)

                                  gCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATgg                                                       <  2:3472537/93‑1 (MQ=255)

                                   cACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATggc                                                      <  1:3103766/93‑1 (MQ=255)

                                   cACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATggc                                                      <  1:7608897/93‑1 (MQ=255)

                                    aCGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATggcg                                                     >  1:2426659/1‑93 (MQ=255)

                                         aaCGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCa                                                >  1:7772767/1‑93 (MQ=255)

                                          aCGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCaa                                               <  1:6524450/93‑1 (MQ=255)

                                          aCGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCaa                                               >  2:3295503/1‑93 (MQ=255)

                                          aCGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCaa                                               <  1:2973273/93‑1 (MQ=255)

                                           cGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAAc                                              <  1:1936671/93‑1 (MQ=255)

                                           cGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCATAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAAc                                              <  1:7414409/93‑1 (MQ=255)

                                             ggTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACAt                                            >  1:5154819/1‑93 (MQ=255)

                                              gTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATc                                           <  2:1660060/93‑1 (MQ=255)

                                              gTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATc                                           >  2:7146570/1‑93 (MQ=255)

                                               tttCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATcc                                          <  2:6168072/93‑1 (MQ=255)

                                               tttCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATcc                                          <  1:6968566/93‑1 (MQ=255)

                                                ttCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCt                                         <  1:1841589/93‑1 (MQ=255)

                                                 tCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTg                                        <  2:1741819/93‑1 (MQ=255)

                                                   gACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGcc                                      <  1:4526722/93‑1 (MQ=255)

                                                   gACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGcc                                      >  1:7448028/1‑93 (MQ=255)

                                                    aCTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCa                                     >  1:478730/1‑93 (MQ=255)

                                                      tttGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCTAAACCAGTTGCTTGTTATGGCGGCGCAACATCCTGCCCGc                                   >  1:1259779/1‑93 (MQ=255)

                                                      tttGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACACTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGc                                   >  1:4703187/1‑93 (MQ=255)

                                                           gTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGt                              <  1:4215181/93‑1 (MQ=255)

                                                           gTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGt                              >  2:463819/1‑93 (MQ=255)

                                                           gTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGt                              <  1:976624/93‑1 (MQ=255)

                                                            ttAATTGATCAGGCTGGTCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTc                             <  2:1902310/93‑1 (MQ=255)

                                                            ttAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTc                             >  2:7310714/1‑93 (MQ=255)

                                                            ttAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTc                             <  1:1441342/93‑1 (MQ=255)

                                                              aaTTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAg                           >  2:1120588/1‑93 (MQ=255)

                                                               aTTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGc                          >  2:6477085/1‑93 (MQ=255)

                                                               aTTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGc                          <  1:5489522/93‑1 (MQ=255)

                                                                  gATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTTCCAGCTTAGTCAGCGTg                       >  2:5174179/1‑93 (MQ=255)

                                                                  gATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTg                       <  1:3167953/93‑1 (MQ=255)

                                                                   aTCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTgc                      <  1:566246/93‑1 (MQ=255)

                                                                   aTCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTgc                      <  1:7276983/93‑1 (MQ=255)

                                                                      aGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGAGcc                   >  2:3204930/1‑93 (MQ=255)

                                                                       ggctgggctgGGTCACGATACCTTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGccg                  <  2:4794856/93‑2 (MQ=255)

                                                                        gctggtctggGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCt                 <  2:2925993/93‑1 (MQ=255)

                                                                        gctgggctggGTCCCGCTCCCCTGGCCCGGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCt                 <  1:6032949/93‑1 (MQ=255)

                                                                          tgggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTaa               <  1:7200261/93‑1 (MQ=255)

                                                                          tgggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTaa               >  2:7498724/1‑93 (MQ=255)

                                                                          tgggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTaa               <  2:7631924/93‑1 (MQ=255)

                                                                           gggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAAt              >  2:5886159/1‑93 (MQ=255)

                                                                            ggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATg             <  1:6869046/93‑1 (MQ=255)

                                                                            ggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATg             <  2:5871379/93‑1 (MQ=255)

                                                                              ctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGc           <  2:7416977/93‑1 (MQ=255)

                                                                                  gTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTgg       >  2:432771/1‑93 (MQ=255)

                                                                                   tCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGa      <  1:3864961/93‑1 (MQ=255)

                                                                                    cACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGaa     <  1:1752005/93‑1 (MQ=255)

                                                                                    cACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGaa     <  2:1786065/93‑1 (MQ=255)

                                                                                    cACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGaa     <  1:5915558/93‑1 (MQ=255)

                                                                                     aCGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAg    >  2:5319852/1‑93 (MQ=255)

ATTCCATTCAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGAC  >  NC_000913/3414656‑3414835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`