Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,109 G→A P152L (CCG→CTG)  rplC ← 50S ribosomal subunit protein L3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,1090GA99.1% 1199.0 / ‑3.6 333P152L (CCG→CTG) rplC50S ribosomal subunit protein L3
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (168/161);  total (170/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 344 total aligned reads displayed.

GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTA  >  NC_000913/3450019‑3450198
                                                                                          |                                                                                         
gCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGa                                                                                         >  2:6983634/1‑93 (MQ=255)
gCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGa                                                                                         >  2:1714800/1‑93 (MQ=255)
   tCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGGTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTc                                                                                      >  1:3427928/1‑93 (MQ=255)
    cAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCt                                                                                     <  1:412874/93‑1 (MQ=255)
    cAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCt                                                                                     >  2:3953189/1‑93 (MQ=255)
      aCGCGTACTACGTCAAGGCTCTGAACGGTTACACTTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTgg                                                                                   <  2:2925275/93‑1 (MQ=255)
      aCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTgg                                                                                   >  1:7901716/1‑93 (MQ=255)
      aCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTgg                                                                                   <  2:1444313/93‑1 (MQ=255)
      aCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTgg                                                                                   <  1:1378850/93‑1 (MQ=255)
       cgcgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGt                                                                                  <  1:5736006/93‑1 (MQ=255)
       cgcgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGt                                                                                  <  1:6952633/93‑1 (MQ=255)
         cgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTc                                                                                <  1:3924962/93‑1 (MQ=255)
           tactacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTg                                                                              >  1:1020728/1‑93 (MQ=255)
              tacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGAcc                                                                           >  2:3186371/1‑93 (MQ=255)
               acGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCg                                                                          >  1:648376/1‑93 (MQ=255)
               acGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCg                                                                          <  1:6614907/93‑1 (MQ=255)
                cGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGa                                                                         >  2:2539959/1‑93 (MQ=255)
                cGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGa                                                                         >  2:2442648/1‑93 (MQ=255)
                cGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGa                                                                         <  2:7649011/93‑1 (MQ=255)
                 gTCAAGGCTCTTAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGAt                                                                        <  2:2033164/93‑1 (MQ=255)
                 gTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGAt                                                                        >  2:4927387/1‑93 (MQ=255)
                  tCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATa                                                                       >  1:5298642/1‑93 (MQ=255)
                  tCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATa                                                                       >  2:3538559/1‑93 (MQ=255)
                   cAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATaa                                                                      >  2:6237016/1‑92 (MQ=255)
                   cAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAg                                                                      >  1:5912933/1‑93 (MQ=255)
                   cAAGGCTCTGAACCGTTCCACGTTAGTTACCCATCTGACCTGCCCTTTTCTTGCCTTTGAACACTTTGCCCACAGTCTAGTTCTGACCGAtca                                                                      >  1:3083889/1‑91 (MQ=255)
                     aGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGaa                                                                    >  1:863635/1‑93 (MQ=255)
                     aGGCCCTGAACCGTTACCCGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGaa                                                                    <  1:5408726/93‑1 (MQ=255)
                      ggCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAc                                                                   <  1:1914103/93‑1 (MQ=255)
                       gCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAcc                                                                  <  2:2301530/93‑1 (MQ=255)
                         tctGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCg                                                                <  1:3611353/93‑1 (MQ=255)
                          ctGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCgg                                                               >  2:3338113/1‑93 (MQ=255)
                           tGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGCTAGAACCCGGa                                                              >  2:5782129/1‑93 (MQ=255)
                            gAACGGTTACACGTTCTTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGaa                                                             <  1:4345357/93‑1 (MQ=255)
                            gAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGACCACTTTGCCCAGAGGCTGGTTCTGACCGATAGAACCCGGaa                                                             >  2:1761046/1‑93 (MQ=255)
                            gAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGaa                                                             <  2:1922066/93‑1 (MQ=255)
                            gAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGaa                                                             <  1:2484618/93‑1 (MQ=255)
                               cGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAcgc                                                          <  2:2161945/93‑1 (MQ=255)
                                ggTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAcgcg                                                         <  1:3216115/93‑1 (MQ=255)
                                ggTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAcgcg                                                         <  2:5325824/93‑1 (MQ=255)
                                 gTTTCACGTACGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAATCCGGAACGCgg                                                        <  1:524594/93‑1 (MQ=255)
                                 gTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCgg                                                        >  2:6609309/1‑93 (MQ=255)
                                 gTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCgg                                                        >  1:3709402/1‑93 (MQ=255)
                                  ttACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCggg                                                       >  2:5016396/1‑92 (MQ=255)
                                  ttACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCggg                                                       >  2:6029678/1‑92 (MQ=255)
                                  ttACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCggg                                                       >  1:7572398/1‑92 (MQ=255)
                                  ttACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGt                                                       <  2:2760103/93‑1 (MQ=255)
                                      ccgttctttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTgaga                                                   <  2:3768937/92‑1 (MQ=255)
                                       cgttcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGAc                                                  <  2:4257786/93‑1 (MQ=255)
                                        gttctttACCCATCAGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACa                                                 <  2:6559291/93‑1 (MQ=255)
                                        gttcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACa                                                 >  1:5542645/1‑93 (MQ=255)
                                         ttcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACaa                                                >  2:7260007/1‑93 (MQ=255)
                                          tcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAg                                               <  1:3490215/93‑1 (MQ=255)
                                           cgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAgg                                              >  1:7421833/1‑93 (MQ=255)
                                            gttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGa                                             <  2:7901715/93‑1 (MQ=255)
                                            gttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGa                                             >  2:6447626/1‑93 (MQ=255)
                                             ttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAg                                            <  1:6434247/93‑1 (MQ=255)
                                             ttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAg                                            <  1:4737133/93‑1 (MQ=255)
                                             ttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAg                                            <  1:2259753/93‑1 (MQ=255)
                                              tACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGt                                           >  2:1383000/1‑93 (MQ=255)
                                              tACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGt                                           >  1:797270/1‑93 (MQ=255)
                                                 ccATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAc                                        >  1:6167568/1‑93 (MQ=255)
                                                 ccATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAc                                        <  2:4081271/93‑1 (MQ=255)
                                                  cATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAcc                                       >  2:6734912/1‑93 (MQ=255)
                                                    tCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGt                                     >  2:1557894/1‑93 (MQ=255)
                                                     cTGGCCTGCCATTTTCTTGCCTTTGAACCCTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTg                                    <  1:380725/93‑1 (MQ=255)
                                                     cTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTg                                    <  1:5780957/93‑1 (MQ=255)
                                                        gccTGCCATTTTCTTGCCTTTTAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGATCCCGGACCGCGGTGAGACAAGGAGTTACCGTGAGt                                 <  1:5507922/92‑1 (MQ=255)
                                                        gccTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGt                                 <  1:4016042/92‑1 (MQ=255)
                                                         ccTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTa                                <  2:2852359/93‑1 (MQ=255)
                                                         ccTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTa                                <  2:5534090/93‑1 (MQ=255)
                                                           tGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGc                              >  2:7396193/1‑93 (MQ=255)
                                                           tGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGc                              >  2:119875/1‑93 (MQ=255)
                                                           cgCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGc                              <  1:4831407/92‑1 (MQ=255)
                                                            gCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCg                             <  1:429214/93‑1 (MQ=255)
                                                              cATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGAGAAGGAGTTACCGTGAGTAGCGTc                           >  2:4283916/1‑93 (MQ=255)
                                                               aTTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTcc                          >  1:1832702/1‑93 (MQ=255)
                                                                ttttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCt                         >  2:773421/1‑93 (MQ=255)
                                                                ttttCTTGCCTTTGAACACTTTGCCCACAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGCCAAGGCGGTACGGTGAACAGCggctt                         >  1:1096132/1‑89 (MQ=255)
                                                                 tttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTg                        <  1:1139402/93‑1 (MQ=255)
                                                                  ttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTgg                       <  1:1295681/93‑1 (MQ=255)
                                                                   tCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTggg                      >  2:1045242/1‑93 (MQ=255)
                                                                   tCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTggg                      >  1:3268446/1‑93 (MQ=255)
                                                                    cTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTggga                     >  2:4879850/1‑92 (MQ=255)
                                                                        ccTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACgg                 >  1:1663679/1‑93 (MQ=255)
                                                                        ccTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACgg                 >  1:7830601/1‑93 (MQ=255)
                                                                         cTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGa                >  1:2140172/1‑93 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGaa               >  2:4726215/1‑93 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGaa               <  2:561002/93‑1 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGaa               <  2:2521020/93‑1 (MQ=255)
                                                                           ttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAg              <  2:904469/93‑1 (MQ=255)
                                                                            tGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGt             >  1:505295/1‑93 (MQ=255)
                                                                              aaCACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTc           >  2:4493465/1‑93 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTcc          <  2:3400109/93‑1 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTcc          <  2:3834548/93‑1 (MQ=255)
                                                                                 acTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAg        <  1:2395870/93‑1 (MQ=255)
                                                                                  cTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgc       >  1:4536473/1‑93 (MQ=255)
                                                                                  cTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgc       <  1:3003088/93‑1 (MQ=255)
                                                                                   tttGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgcg      >  2:7507972/1‑93 (MQ=255)
                                                                                       cccAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTa  <  1:7164309/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTA  >  NC_000913/3450019‑3450198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑