BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,456,098	A→G	Q41Q (CAA→CAG)	yheG →	YheG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,456,098	0	A	G	99.7%	1100.4 / ‑5.4	349	Q41Q (CAA→CAG)	yheG	YheG
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (174/174); total (174/175)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

Only 100 of 352 total aligned reads displayed.

GGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGG  >  NC_000913/3456008‑3456187

    cTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAg                                                                                     >  1:6885839/1‑93 (MQ=255)

      ggCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGt                                                                                   >  1:7037173/1‑93 (MQ=255)

      ggCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGt                                                                                   >  2:6355408/1‑93 (MQ=255)

       gCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGtt                                                                                  <  1:2241701/93‑1 (MQ=255)

        cacaACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTg                                                                                 <  1:3550744/93‑1 (MQ=255)

         acaACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTgg                                                                                >  1:1416447/1‑93 (MQ=255)

         acaACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTgg                                                                                >  1:7478815/1‑93 (MQ=255)

          caACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGc                                                                               >  2:2237158/1‑93 (MQ=255)

          caACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGc                                                                               >  1:2969938/1‑93 (MQ=255)

           aaCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGcc                                                                              >  2:5950485/1‑93 (MQ=255)

            aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCa                                                                             >  1:6328386/1‑93 (MQ=255)

            aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCa                                                                             >  2:3363067/1‑93 (MQ=255)

            aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCa                                                                             <  2:3512270/93‑1 (MQ=255)

            aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCa                                                                             <  1:2364140/93‑1 (MQ=255)

             cGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAg                                                                            <  2:2098286/93‑1 (MQ=255)

             cGAATTGCTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAg                                                                            <  2:1714229/93‑1 (MQ=255)

              gAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGt                                                                           >  1:7731520/1‑93 (MQ=255)

               aaTTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTg                                                                          >  2:4778087/1‑93 (MQ=255)

                aTTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGc                                                                         >  1:4854172/1‑93 (MQ=255)

                  tGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATACGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCa                                                                       <  2:2337124/93‑1 (MQ=255)

                    gTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGa                                                                     <  2:282568/93‑1 (MQ=255)

                    gTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGa                                                                     <  1:2827608/93‑1 (MQ=255)

                     ttATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGAc                                                                    >  2:4665878/1‑93 (MQ=255)

                      tATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGAcc                                                                   >  2:1544491/1‑93 (MQ=255)

                      tATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGAcc                                                                   <  1:6259437/93‑1 (MQ=255)

                        tctcTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAt                                                                 >  1:2426383/1‑93 (MQ=255)

                        tctcTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGACAGTGCCAGACCAt                                                                 >  2:3040299/1‑93 (MQ=255)

                           ctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAc                                                              <  1:409474/93‑1 (MQ=255)

                           ctATTAAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAc                                                              <  2:1628664/93‑1 (MQ=255)

                            tATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAcc                                                             >  1:2011233/1‑93 (MQ=255)

                              ttCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAg                                                           <  1:6218127/93‑1 (MQ=255)

                                cAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCt                                                         >  1:7297742/1‑93 (MQ=255)

                                 agagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                        >  1:3052646/1‑93 (MQ=255)

                                 agagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                        >  1:6007105/1‑93 (MQ=255)

                                 agagGGCGAGATTATTTATCTCGCCAATACGCAATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                        <  1:7190477/93‑1 (MQ=255)

                                    gggCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTc                                                     <  2:4262552/93‑1 (MQ=255)

                                    gggCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTc                                                     <  2:5630967/93‑1 (MQ=255)

                                    gggCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTc                                                     <  2:5740400/93‑1 (MQ=255)

                                     ggCGAGATTATTTATCTCGCCGATACGCTATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGATCATGACCAGCTTGTca                                                    >  2:3718222/1‑93 (MQ=255)

                                       cGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTcaca                                                  <  2:6587021/93‑1 (MQ=255)

                                        gagaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAg                                                 <  1:1888375/93‑1 (MQ=255)

                                        gagaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAg                                                 >  1:1768279/1‑93 (MQ=255)

                                         agaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAgg                                                <  2:6223681/93‑1 (MQ=255)

                                          gaTTATTTACCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGc                                               <  1:5012129/93‑1 (MQ=255)

                                           gttatttatCTCGCCGGTACGCCATTCGGGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCt                                              <  1:6037339/92‑1 (MQ=255)

                                           attattTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCt                                              >  1:4451220/1‑93 (MQ=255)

                                           attattTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCt                                              <  1:1596427/93‑1 (MQ=255)

                                            ttatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTg                                             >  1:5204788/1‑93 (MQ=255)

                                            ttatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTg                                             >  2:1259844/1‑93 (MQ=255)

                                            ttatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTg                                             <  2:3894445/93‑1 (MQ=255)

                                            ttatctatCTCGCGGATGCGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTGGTCAAGTGCCAGACCATGACCAGCTTGTCACAGGCTg                                             <  1:884041/93‑1 (MQ=255)

                                             tatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGa                                            <  2:3655894/93‑1 (MQ=255)

                                             tatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGa                                            >  1:3824501/1‑93 (MQ=255)

                                              atttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAt                                           <  2:710933/93‑1 (MQ=255)

                                              atttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAt                                           >  2:7870854/1‑93 (MQ=255)

                                               tttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAtt                                          <  2:2360216/93‑1 (MQ=255)

                                               tttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAtt                                          >  1:1282118/1‑93 (MQ=255)

                                               tttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAtt                                          >  1:5417952/1‑93 (MQ=255)

                                                 tatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAtttt                                        <  2:7069860/93‑1 (MQ=255)

                                                  atCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTg                                       >  2:6503382/1‑93 (MQ=255)

                                                    ccccccGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGaa                                     <  2:3112766/89‑1 (MQ=255)

                                                       gCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGCTTTTGAAGCt                                  <  2:5090692/93‑1 (MQ=255)

                                                       gCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCt                                  >  2:18420/1‑93 (MQ=255)

                                                         cGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCg                                >  2:1358972/1‑93 (MQ=255)

                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                               >  1:5816296/1‑93 (MQ=255)

                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                               >  1:1021382/1‑93 (MQ=255)

                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                               >  1:4706279/1‑93 (MQ=255)

                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                               >  1:4001519/1‑93 (MQ=255)

                                                           aTACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGc                              >  1:6551159/1‑93 (MQ=255)

                                                              cGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTgg                           >  1:4237563/1‑93 (MQ=255)

                                                               gCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGa                          >  2:7380190/1‑93 (MQ=255)

                                                               gCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGa                          >  1:2653911/1‑93 (MQ=255)

                                                                ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                         <  2:76444/93‑1 (MQ=255)

                                                                ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                         <  2:4991747/93‑1 (MQ=255)

                                                                 cATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGGTCGGCTGGAAg                        <  2:2475962/93‑1 (MQ=255)

                                                                 cATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGCAg                        >  2:4378694/1‑93 (MQ=255)

                                                                  atgcGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGc                       <  2:4091987/90‑1 (MQ=255)

                                                                  aTTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGc                       >  1:3658054/1‑93 (MQ=255)

                                                                  aTTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGc                       <  1:3389605/93‑1 (MQ=255)

                                                                  aTTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGc                       >  1:5265206/1‑93 (MQ=255)

                                                                   ttCGAGCGGTTACTCTATATTCAGCTTCAGGCTGGCCAGTGCCATACCATGTCCAGCTTTTCACATGCTGATTGTGAAGCGCGGCCTGAAGCg                      >  2:7273372/1‑93 (MQ=255)

                                                                   ttCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTTATTTTGAAGCTCGGCTGGAAGCg                      <  2:2723796/93‑1 (MQ=255)

                                                                   ttCGAGCGGTTACTCGATATTCAGCGTCAGGTTGACCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCg                      <  1:7605258/93‑1 (MQ=255)

                                                                    tCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCgg                     <  1:3309687/93‑1 (MQ=255)

                                                                     cGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGt                    >  1:5677642/1‑93 (MQ=255)

                                                                      gAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTa                   <  1:6384510/93‑1 (MQ=255)

                                                                      gAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGGGCCAGACCATGACCAGCTTGTCACAGGCTGCTTTTGAAGCTCGGCTGGAAGCggat                   >  2:2553394/1‑91 (MQ=255)

                                                                           gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa              <  2:6786253/93‑1 (MQ=255)

                                                                           gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCCGCTGGAAGCGGTATTCCa              <  2:7743100/93‑1 (MQ=255)

                                                                            ttACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAt             >  2:79171/1‑93 (MQ=255)

                                                                            ttACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAt             >  1:1921999/1‑93 (MQ=255)

                                                                                 cGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGaa        <  2:609918/93‑1 (MQ=255)

                                                                                  gATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAt       >  1:1790791/1‑93 (MQ=255)

                                                                                  gATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAt       <  2:5364352/93‑1 (MQ=255)

                                                                                   atatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATa      >  2:4508117/1‑93 (MQ=255)

                                                                                   atatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATa      >  2:6323141/1‑93 (MQ=255)

                                                                                   atatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATa      >  2:569375/1‑93 (MQ=255)

                                                                                    tatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAtgc     >  2:911270/1‑91 (MQ=255)

                                                                                    tatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAc     <  1:5072177/93‑1 (MQ=255)

                                                                                    tatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAc     >  1:3957576/1‑93 (MQ=255)

GGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGG  >  NC_000913/3456008‑3456187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`