Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,472,167 A→C V8G (GTA→GGA)  rpsL ← 30S ribosomal subunit protein S12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,472,1670AC99.7% 1052.6 / ‑5.1 336V8G (GTA→GGA) rpsL30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (152/183);  total (152/184)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 351 total aligned reads displayed.

TATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAG  >  NC_000913/3472077‑3472255
                                                                                          |                                                                                        
tataCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTccc                                                                                        >  2:6361932/1‑93 (MQ=255)
tataCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTccc                                                                                        >  2:5353059/1‑93 (MQ=255)
tataCACGAGTACATACGCCACGTTTTTGCGGG‑ATGCTTTCCGCGGCGGCACGGTGCTTTTCGCCACTTTGCCAGCACGTGGTTTGCCTCCCa                                                                                       >  2:7371161/1‑93 (MQ=255)
  taCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAg                                                                                      <  1:5958883/93‑1 (MQ=255)
  taCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAg                                                                                      >  1:6958880/1‑93 (MQ=255)
   acacGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGc                                                                                     <  2:7769392/93‑1 (MQ=255)
    cacGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCt                                                                                    <  2:1135983/93‑1 (MQ=255)
    cacGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCt                                                                                    >  2:5031397/1‑93 (MQ=255)
      cGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCCGCTgg                                                                                  >  1:1333339/1‑93 (MQ=255)
       gAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGt                                                                                 >  2:3001534/1‑93 (MQ=255)
       gAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGt                                                                                 >  2:3848918/1‑93 (MQ=255)
        aGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGtt                                                                                <  2:6828600/93‑1 (MQ=255)
        aGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGtt                                                                                >  2:3784213/1‑93 (MQ=255)
         gTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTa                                                                               >  1:4126203/1‑93 (MQ=255)
          tACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTaa                                                                              >  1:1044636/1‑93 (MQ=255)
          tACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTaa                                                                              >  1:5460481/1‑93 (MQ=255)
          tACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTaa                                                                              <  1:2414151/93‑1 (MQ=255)
           aCATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAAc                                                                             <  1:3440440/93‑1 (MQ=255)
              tACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGt                                                                          >  1:7737830/1‑93 (MQ=255)
              tACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGt                                                                          <  2:1017065/93‑1 (MQ=255)
               aCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGtt                                                                         <  2:4874621/93‑1 (MQ=255)
               aCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGtt                                                                         <  2:705261/93‑1 (MQ=255)
               aCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGtt                                                                         <  2:5517878/93‑1 (MQ=255)
                cGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTg                                                                        <  2:3139714/93‑1 (MQ=255)
                 gCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGc                                                                       >  2:441452/1‑93 (MQ=255)
                   cACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCa                                                                     <  2:7128493/93‑1 (MQ=255)
                   cACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCa                                                                     >  2:5698248/1‑93 (MQ=255)
                    aCGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCAt                                                                    <  2:1838030/93‑1 (MQ=255)
                      gTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTa                                                                  >  1:2566500/1‑93 (MQ=255)
                        ttttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTaaa                                                                <  2:6269823/93‑1 (MQ=255)
                        ttttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTaaa                                                                <  1:2307043/93‑1 (MQ=255)
                        ttttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTaaa                                                                <  2:1400819/93‑1 (MQ=255)
                        ttttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTaaa                                                                <  1:5364784/93‑1 (MQ=255)
                        ttttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTaaa                                                                <  2:3974106/93‑1 (MQ=255)
                                gCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCt                                                        >  1:4792987/1‑93 (MQ=255)
                                gCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCt                                                        <  2:6686698/93‑1 (MQ=255)
                                 cATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTg                                                       <  2:862803/93‑1 (MQ=255)
                                 cATGCTTCCAGCGCAGGCACGTTCCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTg                                                       <  2:5663470/93‑1 (MQ=255)
                                  aTGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTgg                                                      <  2:163374/93‑1 (MQ=255)
                                  aTGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTgg                                                      <  2:6558849/93‑1 (MQ=255)
                                   tGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGt                                                     >  2:7584207/1‑93 (MQ=255)
                                   tGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGt                                                     >  1:1948562/1‑93 (MQ=255)
                                     cTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGttt                                                   >  2:270049/1‑93 (MQ=255)
                                      ttCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGtttt                                                  >  2:767079/1‑93 (MQ=255)
                                       tCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTa                                                 >  2:2407149/1‑93 (MQ=255)
                                         cAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAgt                                               >  1:6266737/1‑92 (MQ=255)
                                           gcgcAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCtt                                             <  1:5640925/93‑1 (MQ=255)
                                           gcgcAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCtt                                             <  2:4177770/93‑1 (MQ=255)
                                             gcAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCtttt                                           >  1:7901476/1‑93 (MQ=255)
                                             gcAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCtttt                                           >  1:2097824/1‑93 (MQ=255)
                                               aGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGc                                         <  1:2932713/93‑1 (MQ=255)
                                               aGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGGTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGc                                         >  1:1261082/1‑93 (MQ=255)
                                                   aCGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCg                                     >  1:7544579/1‑93 (MQ=255)
                                                    cGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGt                                    <  1:3328475/93‑1 (MQ=255)
                                                    cGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGt                                    >  1:586210/1‑93 (MQ=255)
                                                     gTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTa                                   <  2:1918159/93‑1 (MQ=255)
                                                      ttGCTTTTCGCAACTTTGCGATCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTaa                                  <  1:4975414/93‑1 (MQ=255)
                                                      ttGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCTAGCTGCTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTaa                                  <  1:3758527/93‑1 (MQ=255)
                                                      ttGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTaa                                  <  1:4434904/93‑1 (MQ=255)
                                                       tGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTaaa                                 >  2:4974935/1‑93 (MQ=255)
                                                       ggCTTTTCGCAACTGTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCGCCTGGTTTTAGCTTTTGCTTCGTaaa                                 <  1:1057904/92‑1 (MQ=255)
                                                        gCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAac                                <  2:3492124/93‑1 (MQ=255)
                                                         cTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAaca                               <  2:2549805/93‑1 (MQ=255)
                                                         cTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAaca                               <  1:7385320/93‑1 (MQ=255)
                                                         cTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAaca                               >  1:4670061/1‑93 (MQ=255)
                                                         cTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAaca                               >  1:4366129/1‑93 (MQ=255)
                                                          ttttCGCAACTTTGCGAGCACGTGGTTCGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAacac                              >  2:4760694/1‑93 (MQ=255)
                                                           tttCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACg                             <  2:1235213/93‑1 (MQ=255)
                                                           tttCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACg                             <  1:5583131/93‑1 (MQ=255)
                                                           tttCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACg                             >  1:7435307/1‑93 (MQ=255)
                                                            ttCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGt                            <  1:2274008/93‑1 (MQ=255)
                                                              cGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaa                          >  1:7526/1‑93 (MQ=255)
                                                               gCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaat                         <  1:3237740/93‑1 (MQ=255)
                                                                  aCTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATaaa                      <  1:1617375/93‑1 (MQ=255)
                                                                   cTTTGCGAGCACGTGGCTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATaaaa                     <  2:155809/93‑1 (MQ=255)
                                                                    tttGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAAc                    >  1:3129693/1‑93 (MQ=255)
                                                                    tttGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAAc                    <  1:3399844/93‑1 (MQ=255)
                                                                       gCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                 >  2:2774817/1‑93 (MQ=255)
                                                                       gCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                 <  2:6027757/93‑1 (MQ=255)
                                                                       gCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                 <  1:1806908/93‑1 (MQ=255)
                                                                       gCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                 >  2:4571373/1‑93 (MQ=255)
                                                                       gCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                 <  2:2123038/93‑1 (MQ=255)
                                                                         gAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCt               >  2:5476128/1‑93 (MQ=255)
                                                                         gAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCt               <  2:7188010/93‑1 (MQ=255)
                                                                          aGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTc              <  1:6065297/93‑1 (MQ=255)
                                                                          aGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTc              <  2:1041432/93‑1 (MQ=255)
                                                                            cACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcac            >  1:2733021/1‑93 (MQ=255)
                                                                            cACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcac            <  1:5757430/93‑1 (MQ=255)
                                                                             aCGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcaca           >  1:6840420/1‑93 (MQ=255)
                                                                              cGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcacac          <  2:5119296/93‑1 (MQ=255)
                                                                               gTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcacaca         >  1:379350/1‑93 (MQ=255)
                                                                                   tttGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAatat     <  2:4134278/93‑1 (MQ=255)
                                                                                   tttGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAatag     >  1:7208435/1‑92 (MQ=255)
                                                                                   tttGCGTCCCAGCTGGGTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAatat     >  1:482024/1‑93 (MQ=255)
                                                                                    ttGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATg    >  2:5839169/1‑93 (MQ=255)
                                                                                     tGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGa   <  1:349599/93‑1 (MQ=255)
                                                                                     tGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGa   <  2:7862871/93‑1 (MQ=255)
                                                                                      gCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAg  <  2:7290086/93‑1 (MQ=255)
                                                                                      gCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAg  <  2:2297271/93‑1 (MQ=255)
                                                                                      gCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAg  <  1:5138843/93‑1 (MQ=255)
                                                                                          |                                                                                        
TATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAG  >  NC_000913/3472077‑3472255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑