Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,539,774 G→A A578T (GCC→ACC)  feoB → ferrous iron transport protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,539,7740GA99.8% 1470.1 / ‑6.2 420A578T (GCC→ACC) feoBferrous iron transport protein B
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (179/240);  total (180/240)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 425 total aligned reads displayed.

GCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTT  >  NC_000913/3539684‑3539863
                                                                                          |                                                                                         
 cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCa                                                                                        <  2:6849064/93‑1 (MQ=255)
 cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCa                                                                                        <  1:4126212/93‑1 (MQ=255)
  gtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCAt                                                                                       <  2:3042885/93‑1 (MQ=255)
   tccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATg                                                                                      <  2:1378662/93‑1 (MQ=255)
   tccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATg                                                                                      >  2:2851573/1‑93 (MQ=255)
   tccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATg                                                                                      <  1:100479/93‑1 (MQ=255)
   tccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATg                                                                                      >  1:2476087/1‑93 (MQ=255)
       tcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGa                                                                                  >  2:3879166/1‑93 (MQ=255)
       tcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGa                                                                                  >  2:1647811/1‑93 (MQ=255)
       tcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGa                                                                                  <  2:514678/93‑1 (MQ=255)
         aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGaaa                                                                                >  2:7910240/1‑93 (MQ=255)
              ggTGATCCCCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGt                                                                           <  1:4498942/93‑1 (MQ=255)
              ggTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGt                                                                           <  2:4478751/93‑1 (MQ=255)
              ggTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGt                                                                           <  1:700226/93‑1 (MQ=255)
               gTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTg                                                                          <  2:219640/93‑1 (MQ=255)
               gTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTg                                                                          >  2:3368016/1‑93 (MQ=255)
                 gATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGt                                                                        <  2:3584152/93‑1 (MQ=255)
                  aTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCACCGGTTGGTCTGTTTACAGGTACCATGGCGAAAGAAGTGGTa                                                                       <  2:212045/93‑1 (MQ=255)
                  aTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTa                                                                       <  1:5400417/93‑1 (MQ=255)
                  aTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTa                                                                       >  1:2849565/1‑93 (MQ=255)
                   tCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAg                                                                      <  2:6145285/93‑1 (MQ=255)
                   tCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAg                                                                      <  2:7413082/93‑1 (MQ=255)
                   tCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAg                                                                      <  1:4665230/93‑1 (MQ=255)
                   tCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAg                                                                      <  2:4766307/93‑1 (MQ=255)
                    cACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGt                                                                     >  1:982228/1‑93 (MQ=255)
                     aCCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTCGTg                                                                    >  1:6362210/1‑93 (MQ=255)
                     aCCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTg                                                                    <  2:3472263/93‑1 (MQ=255)
                     aCCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTg                                                                    <  1:1240213/93‑1 (MQ=255)
                      cccgGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTgg                                                                   <  1:3146602/93‑1 (MQ=255)
                       cccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTggg                                                                  <  1:6828763/93‑1 (MQ=255)
                         cGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTa                                                                >  1:1564979/1‑93 (MQ=255)
                          ggTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTAc                                                               >  1:4902940/1‑93 (MQ=255)
                           gTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTagg                                                              >  1:5827053/1‑91 (MQ=255)
                           gTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACg                                                              >  2:2298694/1‑93 (MQ=255)
                           gTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGAGGTAGTGGGTACg                                                              >  2:6302369/1‑93 (MQ=255)
                              ttCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTc                                                           >  1:5641282/1‑93 (MQ=255)
                               tCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                                                          >  1:6200669/1‑93 (MQ=255)
                               tCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                                                          >  1:1465353/1‑93 (MQ=255)
                                cAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCaa                                                         >  2:4634347/1‑93 (MQ=255)
                                 aaGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCCac                                                        >  2:5118135/1‑93 (MQ=255)
                                 aaGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                                                        <  2:5864558/93‑1 (MQ=255)
                                  aGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAaca                                                       >  1:7513297/1‑93 (MQ=255)
                                  aGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAaca                                                       <  2:7506036/93‑1 (MQ=255)
                                   gCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAacac                                                      <  2:2576416/93‑1 (MQ=255)
                                   gCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAacac                                                      <  1:5308179/93‑1 (MQ=255)
                                     cAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc                                                    <  2:1243476/93‑1 (MQ=255)
                                     cAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc                                                    <  2:4087486/93‑1 (MQ=255)
                                      aaTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct                                                   >  1:2168275/1‑93 (MQ=255)
                                       aTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc                                                  <  1:2997117/93‑1 (MQ=255)
                                         tGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTa                                                <  1:5974983/93‑1 (MQ=255)
                                           gCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTaca                                              <  2:3274630/93‑1 (MQ=255)
                                            cGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac                                             <  1:185927/93‑1 (MQ=255)
                                             gTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACAcc                                            >  2:3135623/1‑93 (MQ=255)
                                              tGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCg                                           >  2:2017560/1‑93 (MQ=255)
                                                cATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCa                                         >  2:1070587/1‑93 (MQ=255)
                                                cATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCa                                         >  2:7545037/1‑93 (MQ=255)
                                                 aTGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAg                                        >  1:6436328/1‑93 (MQ=255)
                                                 aTGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAg                                        >  2:4334093/1‑93 (MQ=255)
                                                 aTGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAg                                        >  1:1760407/1‑93 (MQ=255)
                                                  tGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa                                       >  2:5455388/1‑93 (MQ=255)
                                                  tGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa                                       >  1:5896331/1‑93 (MQ=255)
                                                  tGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa                                       >  2:4152542/1‑93 (MQ=255)
                                                   gAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACTCTCTACACCGCAGaa                                      >  2:5042868/1‑93 (MQ=255)
                                                   gAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGaa                                      >  2:1797373/1‑93 (MQ=255)
                                                   gAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGaa                                      >  1:1676667/1‑93 (MQ=255)
                                                     aGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGaaaa                                    >  2:1343644/1‑93 (MQ=255)
                                                     aGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGaaaa                                    <  2:3729344/93‑1 (MQ=255)
                                                      gATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAat                                   <  2:147260/93‑1 (MQ=255)
                                                      gATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAat                                   <  1:7351268/93‑1 (MQ=255)
                                                        tAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAatat                                 >  2:6562271/1‑93 (MQ=255)
                                                         aaCTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATAtt                                >  1:2667819/1‑93 (MQ=255)
                                                          accggcaggcaACCGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTc                               <  2:935429/90‑1 (MQ=255)
                                                          aCTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTc                               <  2:7247061/93‑1 (MQ=255)
                                                          aCTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTc                               <  1:4242658/93‑1 (MQ=255)
                                                          aCTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTc                               <  2:6058246/93‑1 (MQ=255)
                                                            tGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAg                             <  2:7760982/93‑1 (MQ=255)
                                                            tGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAg                             >  2:6449993/1‑93 (MQ=255)
                                                             ggcaggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAgg                            <  1:7774546/93‑1 (MQ=255)
                                                             ggcaggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAgg                            >  1:7186702/1‑93 (MQ=255)
                                                             ggcaggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAgg                            <  1:4311447/93‑1 (MQ=255)
                                                              gcaggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGa                           >  1:3294756/1‑93 (MQ=255)
                                                                 ggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGa                        <  2:5935739/93‑1 (MQ=255)
                                                                 ggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGa                        >  1:6243915/1‑93 (MQ=255)
                                                                   caACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAag                      <  1:3541812/93‑1 (MQ=255)
                                                                      cGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGt                   <  2:5734362/93‑1 (MQ=255)
                                                                      cGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGt                   <  1:311845/93‑1 (MQ=255)
                                                                       ggTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGtt                  <  2:2923107/93‑1 (MQ=255)
                                                                       ggTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGtt                  <  2:4923940/93‑1 (MQ=255)
                                                                        gTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGtta                 >  1:2353279/1‑92 (MQ=255)
                                                                        gTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTc                 <  1:3376696/93‑1 (MQ=255)
                                                                         ttGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCa                >  2:1920016/1‑93 (MQ=255)
                                                                          tGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCaa               <  2:6987427/93‑1 (MQ=255)
                                                                            gCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATc             <  2:6366097/93‑1 (MQ=255)
                                                                            gCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATc             <  1:832500/93‑1 (MQ=255)
                                                                             ccTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATcc            >  1:5120415/1‑93 (MQ=255)
                                                                               tGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCgg          >  2:2357007/1‑93 (MQ=255)
                                                                               tGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCgg          >  2:5228535/1‑93 (MQ=255)
                                                                               tGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCgg          >  1:3966275/1‑93 (MQ=255)
                                                                                  ttACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAg       >  1:3834532/1‑93 (MQ=255)
                                                                                    aCAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGaa     <  1:1387511/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTT  >  NC_000913/3539684‑3539863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑