BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,971	T→C	G259G (GGT→GGC)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,971	0	T	C	99.5%	1155.6 / ‑5.5	371	G259G (GGT→GGC)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (163/206); total (164/207)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 381 total aligned reads displayed.

TGGTGCCGGTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGTGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAACGA  >  NC_000913/3544880‑3545060

  gTGCCGGTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGAc                                                                                        <  1:4942095/93‑1 (MQ=255)

   tGCCGGTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTACCGGTAGCGGAGTTCCTCGGCGAcc                                                                                       >  2:2827024/1‑93 (MQ=255)

    gcgcgtagtaCTGATGGCGATGCGTGCGATTGCCTAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGAccc                                                                                      <  1:6987859/89‑1 (MQ=255)

    gCCGGTGGTACTGATGGCGATGGGGGCGATGCCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGAccc                                                                                      <  2:1601400/93‑1 (MQ=255)

      cGGTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCgg                                                                                    <  1:4590134/93‑1 (MQ=255)

       ggTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGt                                                                                   >  1:7531514/1‑93 (MQ=255)

         tagtaCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTaa                                                                                 >  2:331636/1‑93 (MQ=255)

         tagtaCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTaa                                                                                 <  2:5256092/93‑1 (MQ=255)

         tagtaCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTaa                                                                                 >  1:513225/1‑93 (MQ=255)

          agtaCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAAt                                                                                <  2:1380866/93‑1 (MQ=255)

           gtaCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATg                                                                               >  1:4172951/1‑93 (MQ=255)

           gtaCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATg                                                                               <  1:4307349/93‑1 (MQ=255)

            taCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATgg                                                                              >  1:121716/1‑93 (MQ=255)

            taCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATgg                                                                              >  2:1996319/1‑93 (MQ=255)

               tGATGGCGATGGGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGTGACCCGGTAATGGCaa                                                                           <  2:6125265/93‑1 (MQ=255)

               tGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCaa                                                                           <  2:4460711/93‑1 (MQ=255)

               tGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCaa                                                                           >  2:2710988/1‑93 (MQ=255)

               tGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCaa                                                                           >  1:5814717/1‑93 (MQ=255)

               tGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCaa                                                                           >  1:3656694/1‑93 (MQ=255)

               tGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCaa                                                                           >  2:5372009/1‑93 (MQ=255)

                gATGGTGATGCTTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAAc                                                                          <  2:4859370/93‑1 (MQ=255)

                gATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAAc                                                                          <  1:7183191/93‑1 (MQ=255)

                  tGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGc                                                                        <  1:906893/93‑1 (MQ=255)

                    gCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTg                                                                      <  2:6480382/93‑1 (MQ=255)

                     cGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGa                                                                     <  1:6851523/93‑1 (MQ=255)

                     cGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGa                                                                     >  1:7789496/1‑93 (MQ=255)

                      gATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAt                                                                    >  1:3922857/1‑93 (MQ=255)

                      gATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAt                                                                    <  1:3717317/93‑1 (MQ=255)

                       agtcgtgcgAGTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAtt                                                                   <  1:4021624/90‑1 (MQ=255)

                       aTGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAtt                                                                   >  2:2071900/1‑93 (MQ=255)

                         gcgtgcgATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGc                                                                 <  1:5600878/93‑1 (MQ=255)

                         gcgtgcgATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGc                                                                 >  1:4466788/1‑93 (MQ=255)

                            tgcgATTGCCAAAATGATCCTGCCGAAAGGTCACGCTTCCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGt                                                              <  1:3255826/93‑1 (MQ=255)

                             gcgATTGCCGAAATGATCCTGCGGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTg                                                             <  1:1025921/93‑1 (MQ=255)

                               gATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTTCCGTGcg                                                           >  2:3408269/1‑92 (MQ=255)

                               gATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCt                                                           >  1:5053923/1‑93 (MQ=255)

                               gATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCt                                                           >  1:3196106/1‑93 (MQ=255)

                                 ttGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGa                                                         >  2:1913568/1‑93 (MQ=255)

                                 ttGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGa                                                         >  1:309722/1‑93 (MQ=255)

                                 ttGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGa                                                         >  2:3493139/1‑93 (MQ=255)

                                 cttccGAAATGATCCGACCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTCCCTCGGCGACCCGGTAATGGCTACGCTGATTGCCGTGCTGa                                                         <  1:5799581/90‑1 (MQ=255)

                                   gCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGAtt                                                       <  2:7589906/93‑1 (MQ=255)

                                   gCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGAtt                                                       >  1:2332198/1‑93 (MQ=255)

                                   gCCAAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGAtt                                                       <  1:635649/93‑1 (MQ=255)

                                    ccGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTg                                                      <  2:7843772/93‑1 (MQ=255)

                                      gAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCg                                                    >  1:6304354/1‑93 (MQ=255)

                                      gAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCg                                                    <  2:6864359/93‑1 (MQ=255)

                                       aaaTGATCCTGCCGAAAGGTCACGCTTCCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGa                                                   <  1:384603/93‑1 (MQ=255)

                                        aaTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGAt                                                  <  2:7491158/93‑1 (MQ=255)

                                        aaTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGAt                                                  <  2:3865835/93‑1 (MQ=255)

                                        aaTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGAt                                                  >  2:1806292/1‑93 (MQ=255)

                                         aTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATg                                                 >  1:5947359/1‑93 (MQ=255)

                                         aTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATg                                                 <  2:7061390/93‑1 (MQ=255)

                                          tGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGt                                                >  2:1119230/1‑93 (MQ=255)

                                          tGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGt                                                >  1:6206940/1‑93 (MQ=255)

                                             tCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGCTCCTTGGCGACCCGGCAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCa                                             >  2:4328936/1‑93 (MQ=255)

                                              ccTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCAc                                            <  2:2470076/93‑1 (MQ=255)

                                               cTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCAcc                                           >  2:1770822/1‑93 (MQ=255)

                                               cTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCAcc                                           >  2:4994388/1‑93 (MQ=255)

                                                tGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCt                                          >  2:7692804/1‑93 (MQ=255)

                                                  ccGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCttt                                        <  1:2680745/93‑1 (MQ=255)

                                                   cGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTg                                       >  1:5444183/1‑93 (MQ=255)

                                                   cGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTg                                       <  2:5112224/93‑1 (MQ=255)

                                                   cGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTg                                       >  1:4388298/1‑93 (MQ=255)

                                                    gAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTgg                                      >  2:7445833/1‑93 (MQ=255)

                                                    gAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTgg                                      <  1:2421313/93‑1 (MQ=255)

                                                    gAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTgg                                      <  1:5367965/93‑1 (MQ=255)

                                                     aaaGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGt                                     <  2:3716808/93‑1 (MQ=255)

                                                     aaaGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGt                                     >  2:7405139/1‑93 (MQ=255)

                                                      aaGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTc                                    <  1:1597814/93‑1 (MQ=255)

                                                      aaGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTc                                    <  2:7264888/93‑1 (MQ=255)

                                                        ggTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTa                                  <  2:554314/93‑1 (MQ=255)

                                                         gTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTaa                                 >  2:3705502/1‑93 (MQ=255)

                                                          tCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTaaa                                >  1:4861736/1‑93 (MQ=255)

                                                           cACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAAc                               <  1:7880130/93‑1 (MQ=255)

                                                              gCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGt                            >  2:6666837/1‑93 (MQ=255)

                                                              gCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGt                            <  1:3382333/93‑1 (MQ=255)

                                                                tttCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCgggg                          >  1:1865717/1‑93 (MQ=255)

                                                                   ccTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCg                       <  2:243403/93‑1 (MQ=255)

                                                                    cTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGt                      <  2:2949653/93‑1 (MQ=255)

                                                                    cTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGCTCTCCTTTGGTCTAACGCGTGGGCGt                      >  2:5697376/1‑93 (MQ=255)

                                                                     tGCCGGTAGCGGAGTTCCTCGGCGGCCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGtt                     <  2:6272009/93‑1 (MQ=255)

                                                                     tGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGtt                     <  2:3972257/93‑1 (MQ=255)

                                                                        cGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCaa                  <  1:213730/93‑1 (MQ=255)

                                                                        cGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCaa                  >  2:405590/1‑93 (MQ=255)

                                                                         ggTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAAt                 <  2:837528/93‑1 (MQ=255)

                                                                         ggTAGCGGAGTTCCTCGACGACCCGGTAATGGCAACGCTAAATGCCGAGCTGATTGCGATGTTCTCCTTTGGTCTAAACCGTGGTCGTTCAAt                 <  2:7017985/93‑1 (MQ=255)

                                                                          gTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATg                >  2:7082711/1‑93 (MQ=255)

                                                                          gTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATg                <  1:6054534/93‑1 (MQ=255)

                                                                            aGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGa              <  2:4674523/93‑1 (MQ=255)

                                                                            aGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGa              <  2:145738/93‑1 (MQ=255)

                                                                             gCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGAt             >  2:6052704/1‑93 (MQ=255)

                                                                             gCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGAt             <  2:5791219/93‑1 (MQ=255)

                                                                             gCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGAt             <  1:7057752/93‑1 (MQ=255)

                                                                              cGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATc            <  1:734431/93‑1 (MQ=255)

                                                                                 aGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTTCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGa         <  1:6467174/93‑1 (MQ=255)

                                                                                    tCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTa      >  1:2609286/1‑93 (MQ=255)

                                                                                     ccTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTaa     <  1:2595211/93‑1 (MQ=255)

                                                                                      cTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAAc    <  2:5201926/93‑1 (MQ=255)

                                                                                      cTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAAc    <  2:4789830/93‑1 (MQ=255)

TGGTGCCGGTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGTGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAACGA  >  NC_000913/3544880‑3545060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`