BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,582,668	G→A	L93L (TTG→TTA)	yrhB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,582,668	0	G	A	98.3%	1014.5 / NA	290	L93L (TTG→TTA)	yrhB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (209/75); minor base C (0/5); total (210/80)
Fisher's exact test for biased strand distribution p-value = 1.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 322 total aligned reads displayed.

AGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCG  >  NC_000913/3582578‑3582757

aGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTAcc                                                                                         >  2:7175963/1‑93 (MQ=255)

aGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTAcc                                                                                         <  1:2568375/93‑1 (MQ=255)

 gACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTAccc                                                                                        >  1:4282583/1‑93 (MQ=255)

 gACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTAccc                                                                                        >  1:3634777/1‑93 (MQ=255)

  aCAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCt                                                                                       <  1:5848009/93‑1 (MQ=255)

   cAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTg                                                                                      >  1:7503466/1‑93 (MQ=255)

    aGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGa                                                                                     <  2:2550682/93‑1 (MQ=255)

    aGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGa                                                                                     <  1:3148684/93‑1 (MQ=255)

     gtggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGAc                                                                                    >  2:656894/1‑93 (MQ=255)

        gtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACaaa                                                                                 <  2:2737880/93‑1 (MQ=255)

        gtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACaaa                                                                                 >  2:7584684/1‑93 (MQ=255)

         tgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACaaaa                                                                                >  1:7285680/1‑93 (MQ=255)

          gAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAAt                                                                               <  2:4056484/93‑1 (MQ=255)

           aaaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATa                                                                              <  2:7399902/93‑1 (MQ=255)

            aaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAg                                                                             >  1:3992661/1‑93 (MQ=255)

             attcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGc                                                                            >  2:3619981/1‑93 (MQ=255)

             attcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGc                                                                            >  2:4235531/1‑93 (MQ=255)

              ttcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAgac                                                                           >  2:5177828/1‑91 (MQ=255)

              ttcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGcc                                                                           <  1:729854/93‑1 (MQ=255)

              ttcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGcc                                                                           <  2:5358167/93‑1 (MQ=255)

              ttcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGcc                                                                           >  1:1032977/1‑93 (MQ=255)

              ttcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGcc                                                                           >  2:1892327/1‑93 (MQ=255)

               tcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGccc                                                                          >  1:6451498/1‑93 (MQ=255)

                cattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCct                                                                         <  2:7328899/93‑1 (MQ=255)

                cattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCct                                                                         >  1:5394585/1‑93 (MQ=255)

                 attcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCctc                                                                        <  2:4544727/93‑1 (MQ=255)

                  ttcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCctct                                                                       >  2:5476307/1‑93 (MQ=255)

                    ctctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTc                                                                     <  2:678281/93‑1 (MQ=255)

                    ctctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAACAGCCCTCTTc                                                                     >  2:2181087/1‑93 (MQ=255)

                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcc                                                                    <  2:4648414/93‑1 (MQ=255)

                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcc                                                                    <  1:6968061/93‑1 (MQ=255)

                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcc                                                                    >  2:1816863/1‑93 (MQ=255)

                       tGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcccc                                                                  >  1:2187705/1‑92 (MQ=255)

                       tGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCa                                                                  >  1:2265503/1‑93 (MQ=255)

                             cGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAaga                                                            <  1:4590478/93‑1 (MQ=255)

                             cGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAaga                                                            >  1:7656289/1‑93 (MQ=255)

                             cGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAaga                                                            <  1:6214839/93‑1 (MQ=255)

                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                           >  2:470340/1‑93 (MQ=255)

                               gCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAgg                                                          >  1:1512361/1‑93 (MQ=255)

                               gCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAgg                                                          >  1:4824616/1‑93 (MQ=255)

                               gCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAgg                                                          <  1:4047599/93‑1 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                        <  1:6273711/93‑1 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                        >  1:6101649/1‑93 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                        >  1:2773486/1‑93 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                        <  2:6377521/93‑1 (MQ=255)

                                  aaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGcc                                                       >  1:1728461/1‑93 (MQ=255)

                                   aaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCg                                                      <  1:734627/93‑1 (MQ=255)

                                    aCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGc                                                     <  1:7780710/93‑1 (MQ=255)

                                       gCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTaa                                                  >  2:5909541/1‑93 (MQ=255)

                                        cTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAAc                                                 >  2:6681441/1‑93 (MQ=255)

                                        cTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAAc                                                 >  2:3282770/1‑93 (MQ=255)

                                          ggAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCt                                               >  1:6819866/1‑93 (MQ=255)

                                            aagaaTATCTACAGGATTACGAAATAAAAAAGC‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTa                                             <  1:121847/93‑1 (MQ=255)

                                             agaaTATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                            >  1:1788013/1‑93 (MQ=255)

                                              aaaTATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGt                                           <  2:3050709/92‑1 (MQ=255)

                                               aaTATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTa                                          <  2:3933329/93‑1 (MQ=255)

                                               aaTATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTa                                          >  2:568661/1‑93 (MQ=255)

                                                atatCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAc                                         >  2:4454988/1‑93 (MQ=255)

                                                atatCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAc                                         >  2:4746794/1‑93 (MQ=255)

                                                 tatCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAcc                                        >  1:5985933/1‑93 (MQ=255)

                                                  atCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAccc                                       <  1:86030/93‑1 (MQ=255)

                                                   tCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAcccc                                      >  1:4487121/1‑93 (MQ=255)

                                                   tCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAcccc                                      >  1:2450129/1‑93 (MQ=255)

                                                      aCAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgcc                                   >  2:7906717/1‑93 (MQ=255)

                                                      aCAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgcc                                   >  1:5260432/1‑93 (MQ=255)

                                                         ggATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                                >  1:2359339/1‑93 (MQ=255)

                                                         ggATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                                >  1:5469093/1‑93 (MQ=255)

                                                         ggATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                                >  1:3483516/1‑93 (MQ=255)

                                                          gATTACGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTa                               >  1:3925018/1‑93 (MQ=255)

                                                            ttACGAAATAAAAAAGG‑CTCCCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTaaa                             <  2:6176522/93‑1 (MQ=255)

                                                              aCGAAATAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatc                           >  1:294999/1‑93 (MQ=255)

                                                                aaaaaaaaaaaGG‑CCCCCTTCGCCTCCCCCTAAAAAAATCCCCCTCCCCCCAGAAAGAGGCCCGCTAACCTTAGTCCCCCGCCGTTAAatcat                         <  2:1469624/92‑1 (MQ=255)

                                                                 aaaTAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcatc                        >  2:5866647/1‑93 (MQ=255)

                                                                  actAAAAAAGG‑CTACCTGCGGTTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATcc                       <  1:1961491/91‑1 (MQ=255)

                                                                  aaTAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                       >  1:66913/1‑93 (MQ=255)

                                                                  aaTAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                       >  1:3797838/1‑93 (MQ=255)

                                                                   aaaaaaaaGGCCTCCCTTCGGCTTCCCCTAAAAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                       <  2:374079/91‑1 (MQ=255)

                                                                   aTAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCa                      >  1:280882/1‑93 (MQ=255)

                                                                    tAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCAc                     >  1:713022/1‑93 (MQ=255)

                                                                    tAAAAAAGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCAc                     >  2:2368942/1‑93 (MQ=255)

                                                                     aaaaaaGG‑CTACCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACTTTAGTCCCCCGCCGTTAAATCATCCAcc                    <  2:3354719/93‑1 (MQ=255)

                                                                     aaaaaaGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCAcc                    >  2:7782275/1‑93 (MQ=255)

                                                                       aaaaGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGa                  >  2:6943725/1‑93 (MQ=255)

                                                                        aaaGC‑CTCCCTTCGGCTTCCCCTGACAAAATACCCCTCTCCCCACGAAGAGGCCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAg                 <  2:7149788/93‑1 (MQ=255)

                                                                         aaGG‑CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgc                >  2:4949591/1‑93 (MQ=255)

                                                                          aGG‑CTACCTTCGGCTTACCCCGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg               >  2:1390423/1‑93 (MQ=255)

                                                                               tACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCggg           >  1:6741557/1‑93 (MQ=255)

                                                                               tACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCggg           <  2:3513834/93‑1 (MQ=255)

                                                                               tACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCggg           >  1:2066758/1‑93 (MQ=255)

                                                                               tACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGACCGCggg           >  1:5797805/1‑93 (MQ=255)

                                                                                 ccTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTc         >  2:4009801/1‑93 (MQ=255)

                                                                                  cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg        >  2:1157912/1‑93 (MQ=255)

                                                                                  cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg        >  2:1018090/1‑93 (MQ=255)

                                                                                  cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg        >  1:1297143/1‑93 (MQ=255)

                                                                                  cTTCGGCTTACCCTGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg        <  2:2023766/93‑1 (MQ=255)

                                                                                    tgggCTTCCCCTAAAAAAATGGCCCCTTCCCCACGAAGAGGCCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGa      <  2:1656203/91‑1 (MQ=255)

                                                                                    tCGGCTTACCCTAACAAAATAGCCCTCTTCCCAGGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGa      <  2:7690515/93‑1 (MQ=255)

                                                                                    tCGCCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGa      <  2:2808363/93‑1 (MQ=255)

                                                                                      ggCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATg    >  2:2285752/1‑93 (MQ=255)

                                                                                       gCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgc   >  2:5958274/1‑93 (MQ=255)

AGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCG  >  NC_000913/3582578‑3582757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`