BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	384,616	A→G	D73G (GAC→GGC)	tauA →	TauA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	384,616	0	A	G	99.7%	939.4 / ‑5.2	300	D73G (GAC→GGC)	tauA	TauA
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (154/145); total (154/146)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.01e-01

Only 100 of 313 total aligned reads displayed.

ACGTCACCGTGGCGTATCAAACCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAG  >  NC_000913/384526‑384705

aCGTCACCGTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCt                                                                                         >  1:1006744/1‑93 (MQ=255)

aCGTCACCGTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCt                                                                                         >  2:2484386/1‑93 (MQ=255)

 cGTCACCGTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTg                                                                                        <  2:534721/93‑1 (MQ=255)

   tCACCGTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGc                                                                                      >  1:7248375/1‑93 (MQ=255)

   tCACCGTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGc                                                                                      <  1:2872487/93‑1 (MQ=255)

     aCCGTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGt                                                                                    >  1:7715331/1‑93 (MQ=255)

      ccGTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTa                                                                                   >  1:5974806/1‑93 (MQ=255)

      ccGTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAAGCCGACAACACCTCTGCTAAAGAAAGCGGAGCAACCGTGTGCTGGCGTa                                                                                   >  2:326102/1‑93 (MQ=255)

       cGTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTaa                                                                                  <  1:1226031/93‑1 (MQ=255)

       cGTGGCGTATCAAGCCCCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTaa                                                                                  <  1:4356299/93‑1 (MQ=255)

        gTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAg                                                                                 >  2:3813091/1‑93 (MQ=255)

        gTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAg                                                                                 >  1:1616765/1‑93 (MQ=255)

        gTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAg                                                                                 <  2:2353616/93‑1 (MQ=255)

        gTGGCGTATCAAGCCTCAGCCGAACCGGCGAAAGGGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAg                                                                                 >  2:6496493/1‑93 (MQ=255)

           gCGTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGttt                                                                              <  1:4001853/93‑1 (MQ=255)

             gTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGa                                                                            >  2:4562373/1‑93 (MQ=255)

             gTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGa                                                                            <  1:7341464/93‑1 (MQ=255)

             gTATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGa                                                                            >  2:1962226/1‑93 (MQ=255)

              taccAAGCCGCAGCCGACCCGGCGAACGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGAc                                                                           <  2:279926/90‑1 (MQ=255)

              tATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGAc                                                                           <  1:3247810/93‑1 (MQ=255)

              tATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGAc                                                                           <  1:7128808/93‑1 (MQ=255)

              tATCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGCTTGAc                                                                           >  1:2021201/1‑93 (MQ=255)

               aTCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACa                                                                          >  1:6861007/1‑93 (MQ=255)

               aTCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACa                                                                          <  1:3201871/93‑1 (MQ=255)

                tCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAg                                                                         >  1:5607762/1‑93 (MQ=255)

                tCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAg                                                                         >  2:3157940/1‑93 (MQ=255)

                tCAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAg                                                                         >  2:4706343/1‑93 (MQ=255)

                 cAAGCCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGc                                                                        <  2:1340908/93‑1 (MQ=255)

                  aagccTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCg                                                                       >  2:6973982/4‑93 (MQ=255)

                   agccTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCgg                                                                      >  1:5734105/3‑93 (MQ=255)

                    gccTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGa                                                                     <  2:1249906/92‑1 (MQ=255)

                    gccTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGa                                                                     <  1:5017792/92‑1 (MQ=255)

                    gccTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGGGGGATGGCGGAAGTTTGACAGCGGa                                                                     >  2:5878475/2‑93 (MQ=255)

                       tCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGcc                                                                  >  2:5827154/1‑93 (MQ=255)

                        cAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCa                                                                 <  1:6925903/93‑1 (MQ=255)

                          gCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGc                                                               <  2:3985108/93‑1 (MQ=255)

                          gCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGc                                                               >  2:75839/1‑93 (MQ=255)

                            cGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCAt                                                             <  2:7099058/93‑1 (MQ=255)

                             gAACCGGCGAAAGTGGCTCAGGCCGACACCCCCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATc                                                            <  1:6536670/93‑1 (MQ=255)

                             gAACCGGCGAAAGTGGCTCAGGCCGACAAGACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGGTTGACAGCGGAGGCAGCAta                                                            >  1:2862297/1‑92 (MQ=255)

                              aaCCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCg                                                           >  2:672081/1‑93 (MQ=255)

                               aCCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGt                                                          >  1:5302688/1‑93 (MQ=255)

                               aCCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGt                                                          <  1:6709236/93‑1 (MQ=255)

                                ccGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTg                                                         <  1:7936900/93‑1 (MQ=255)

                                  ggCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCg                                                       <  1:6106436/93‑1 (MQ=255)

                                  ggCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCg                                                       <  1:3973745/93‑1 (MQ=255)

                                   gCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCgg                                                      >  2:7384894/1‑93 (MQ=255)

                                     gAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGgc                                                    >  2:5000048/1‑93 (MQ=255)

                                      aaaGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGgcg                                                   >  1:2651050/1‑93 (MQ=255)

                                         gTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTg                                                <  1:5101782/93‑1 (MQ=255)

                                          tGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTgg                                               <  1:2328882/93‑1 (MQ=255)

                                             cTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCtt                                            >  1:5880547/1‑93 (MQ=255)

                                              tCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTc                                           <  1:3816274/93‑1 (MQ=255)

                                              tCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTc                                           <  2:6572691/93‑1 (MQ=255)

                                              tCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTc                                           <  2:7453207/93‑1 (MQ=255)

                                              tCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTc                                           >  2:3886704/1‑93 (MQ=255)

                                                aGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAg                                         >  1:5529479/1‑93 (MQ=255)

                                                 ggCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAgg                                        <  2:7627183/93‑1 (MQ=255)

                                                 ggCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAgg                                        <  1:680634/93‑1 (MQ=255)

                                                 ggCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAgg                                        <  2:2995359/93‑1 (MQ=255)

                                                 ggCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAgg                                        >  2:6400308/1‑93 (MQ=255)

                                                   ccGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCg                                      >  1:492692/1‑93 (MQ=255)

                                                   ccGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCg                                      <  1:6471965/93‑1 (MQ=255)

                                                     gACACCACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTTACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGAc                                    <  2:1665750/93‑1 (MQ=255)

                                                     gACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGAc                                    >  2:3077404/1‑93 (MQ=255)

                                                     gACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGAc                                    >  2:5679330/1‑93 (MQ=255)

                                                     gACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGAc                                    >  2:4237547/1‑93 (MQ=255)

                                                      acaacaCCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACg                                   <  1:970871/93‑1 (MQ=255)

                                                       caacaCCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGt                                  <  2:4785366/93‑1 (MQ=255)

                                                        aacaCCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTg                                 >  2:2577395/1‑93 (MQ=255)

                                                        aacaCCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTg                                 >  1:1858789/1‑93 (MQ=255)

                                                        aacaCCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTg                                 >  2:4347168/1‑93 (MQ=255)

                                                         acacCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGc                                <  1:2420261/93‑1 (MQ=255)

                                                         acacCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGc                                >  1:6180217/1‑93 (MQ=255)

                                                         acacCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGc                                >  2:7860194/1‑93 (MQ=255)

                                                         acacCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGc                                <  2:482010/93‑1 (MQ=255)

                                                           acCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCaa                              >  1:4163994/1‑93 (MQ=255)

                                                           acCTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCaa                              >  1:4555811/1‑93 (MQ=255)

                                                            ccTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCaaa                             >  2:6693467/1‑93 (MQ=255)

                                                            ccTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCaaa                             >  1:865892/1‑93 (MQ=255)

                                                            ccTTTGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCaaa                             >  1:2397318/1‑93 (MQ=255)

                                                              tttGCTAAAGAAAGCGGAGCATCCGGGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGGTGGCTTCAGGCGACGTGCAAATc                           <  2:191058/93‑1 (MQ=255)

                                                              tttGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATc                           >  1:143390/1‑93 (MQ=255)

                                                               ttGCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCg                          <  1:3494580/93‑1 (MQ=255)

                                                                 tcTAAAGAAAGCGGAGCAACCGTGGGCTGGCATAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGc                        <  2:2044257/92‑1 (MQ=255)

                                                                 gCTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGc                        <  1:1356432/93‑1 (MQ=255)

                                                                  cTAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCa                       >  1:4823637/1‑93 (MQ=255)

                                                                   tAAAGAAAGCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCaa                      >  1:4729468/1‑93 (MQ=255)

                                                                      agaaagCGGAGCAACCGTGGTCTGGCTTAAGTTTGACAGCGGAGCCAGCATCGTGCGGTCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCt                   <  2:3727055/93‑1 (MQ=255)

                                                                       gaaagCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTc                  <  2:5170279/93‑1 (MQ=255)

                                                                         aagCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCgg                <  1:1164609/93‑1 (MQ=255)

                                                                          agCGGAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGt               <  1:3614808/93‑1 (MQ=255)

                                                                             ggAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTcc            >  2:1461240/1‑93 (MQ=255)

                                                                             ggAGCAACCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTcc            <  2:6022152/93‑1 (MQ=255)

                                                                                  aaCCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGccc       >  1:4212020/1‑93 (MQ=255)

                                                                                  aaCCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGccc       >  2:6180219/1‑93 (MQ=255)

                                                                                   aCCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCg      >  2:5913771/1‑93 (MQ=255)

                                                                                   aCCGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCg      >  2:3672839/1‑93 (MQ=255)

                                                                                     cGTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGtt    >  2:3398821/1‑93 (MQ=255)

                                                                                      gTGGGCTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTa   <  1:7841033/93‑1 (MQ=255)

ACGTCACCGTGGCGTATCAAACCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAG  >  NC_000913/384526‑384705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`