BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,596,295	A→G	V332A (GTG→GCG)	b3460 ←	LivJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,596,295	0	A	G	99.8%	1378.3 / ‑5.3	437	V332A (GTG→GCG)	b3460	LivJ
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (185/250); total (187/250)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

Only 100 of 456 total aligned reads displayed.

GTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG  >  NC_000913/3596205‑3596384

gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         <  2:2492297/93‑1 (MQ=255)

gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         >  1:5650071/1‑93 (MQ=255)

gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         >  1:4962489/1‑93 (MQ=255)

 tgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCgg                                                                                        >  2:6439960/1‑93 (MQ=255)

 tgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCgg                                                                                        >  1:1701277/1‑93 (MQ=255)

  gccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGa                                                                                       <  1:7776490/93‑1 (MQ=255)

     gtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGtt                                                                                    <  1:2497639/93‑1 (MQ=255)

     gtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGtt                                                                                    <  2:7518773/93‑1 (MQ=255)

      ttGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTc                                                                                   >  1:2720715/1‑93 (MQ=255)

          cGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCtt                                                                               <  2:2840704/93‑1 (MQ=255)

           gTGCCAGTCAAATACGCCGAACTGAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCttt                                                                              <  2:5639191/93‑1 (MQ=255)

           gTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCttt                                                                              >  1:1801458/1‑93 (MQ=255)

           gTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCttt                                                                              >  1:3996151/1‑93 (MQ=255)

            tGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTc                                                                             <  1:4784493/93‑1 (MQ=255)

            tGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTc                                                                             <  1:4084247/93‑1 (MQ=255)

            tGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTc                                                                             >  1:3065465/1‑93 (MQ=255)

             gCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCa                                                                            <  2:2348492/93‑1 (MQ=255)

                aGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGt                                                                         >  1:1011369/1‑93 (MQ=255)

                    aaaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAttt                                                                     <  2:310826/93‑1 (MQ=255)

                    aaaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAttt                                                                     <  2:6152261/93‑1 (MQ=255)

                     aaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTg                                                                    >  2:635322/1‑93 (MQ=255)

                      aTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTgg                                                                   >  2:6235569/1‑93 (MQ=255)

                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  1:590869/93‑1 (MQ=255)

                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  >  2:6783545/1‑93 (MQ=255)

                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  2:1662902/93‑1 (MQ=255)

                         cGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGa                                                                <  2:7276606/93‑1 (MQ=255)

                          gCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAt                                                               >  2:3161058/1‑93 (MQ=255)

                          gCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAt                                                               <  1:5319891/93‑1 (MQ=255)

                            cGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAttt                                                             >  1:4230296/1‑93 (MQ=255)

                             gAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTc                                                            >  2:5559704/1‑93 (MQ=255)

                              aaCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                                           <  1:3323746/93‑1 (MQ=255)

                               aCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAg                                                          >  2:6629172/1‑93 (MQ=255)

                               aCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAg                                                          >  1:6379299/1‑93 (MQ=255)

                                cTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGc                                                         >  1:2811682/1‑93 (MQ=255)

                                cTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGc                                                         <  1:3786312/93‑1 (MQ=255)

                                  cAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                                       >  2:6062817/1‑93 (MQ=255)

                                   aaaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCgg                                                      <  1:1450578/93‑1 (MQ=255)

                                    aaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                                     >  2:5197419/1‑93 (MQ=255)

                                    aaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                                     >  2:5060873/1‑93 (MQ=255)

                                    aaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                                     <  2:6997941/93‑1 (MQ=255)

                                     aGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAt                                                    <  1:7506753/93‑1 (MQ=255)

                                       ccTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCg                                                  <  1:232429/93‑1 (MQ=255)

                                       ccTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCg                                                  <  2:3819866/93‑1 (MQ=255)

                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                <  1:6719925/93‑1 (MQ=255)

                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                >  2:2053954/1‑93 (MQ=255)

                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                >  2:4260076/1‑93 (MQ=255)

                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                >  2:7883967/1‑93 (MQ=255)

                                          ttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCa                                               <  2:2631481/93‑1 (MQ=255)

                                           tCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAg                                              >  1:2047904/1‑93 (MQ=255)

                                            cAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGa                                             >  1:606571/1‑93 (MQ=255)

                                              gATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACt                                           >  1:1753472/1‑93 (MQ=255)

                                               aTCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                                          <  1:114151/93‑1 (MQ=255)

                                                tCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                                         <  2:3979908/93‑1 (MQ=255)

                                                tCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                                         <  1:1205958/93‑1 (MQ=255)

                                                tCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGGATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                                         >  1:7406613/1‑93 (MQ=255)

                                                 cGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCTCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                                        >  1:3747059/1‑93 (MQ=255)

                                                  gCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                                       >  1:3466685/1‑93 (MQ=255)

                                                  gCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                                       <  1:415481/93‑1 (MQ=255)

                                                  gCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                                       <  1:5071064/93‑1 (MQ=255)

                                                  gCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                                       <  2:1031143/93‑1 (MQ=255)

                                                   ccTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTc                                      <  1:3577155/93‑1 (MQ=255)

                                                   ccTTTCTCATCCCAGGTCAGCGGCCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTc                                      <  2:1265496/93‑1 (MQ=255)

                                                    cTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                                     <  1:2928904/93‑1 (MQ=255)

                                                    cTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                                     <  1:5567753/93‑1 (MQ=255)

                                                    cTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                                     <  1:368167/93‑1 (MQ=255)

                                                    cTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAATTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                                     <  1:1027563/93‑1 (MQ=255)

                                                     tttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                                    <  1:163554/93‑1 (MQ=255)

                                                     tttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                                    >  1:1899370/1‑93 (MQ=255)

                                                     tttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                                    >  1:2401838/1‑93 (MQ=255)

                                                       tctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGc                                  <  1:69618/93‑1 (MQ=255)

                                                       tctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGc                                  >  2:3752388/1‑93 (MQ=255)

                                                        ctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGcc                                 <  2:7460567/93‑1 (MQ=255)

                                                            tCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGcc                             <  2:4087343/93‑1 (MQ=255)

                                                            tCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGcc                             >  2:4426797/1‑93 (MQ=255)

                                                               cAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGc                          >  2:3514853/1‑93 (MQ=255)

                                                               cAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGc                          >  1:383617/1‑93 (MQ=255)

                                                                 ggTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaa                        >  1:6563858/1‑93 (MQ=255)

                                                                 cgTCAGCGGTCACATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGGGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaa                        <  2:5933206/92‑1 (MQ=255)

                                                                  gTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaaa                       >  2:5146019/1‑93 (MQ=255)

                                                                  gTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaaa                       >  1:7914643/1‑93 (MQ=255)

                                                                    cAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa                     <  1:724824/93‑1 (MQ=255)

                                                                    cAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa                     <  1:927068/93‑1 (MQ=255)

                                                                      gCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAtt                   <  2:4758846/93‑1 (MQ=255)

                                                                      gCGGTCCCATTACGGGATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCACGCCCGCAAAGAtt                   >  2:578869/1‑93 (MQ=255)

                                                                        ggTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAGTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGc                 <  2:4618925/93‑1 (MQ=255)

                                                                         gTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa                <  2:876699/93‑1 (MQ=255)

                                                                         gTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa                >  2:1542289/1‑93 (MQ=255)

                                                                         gTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa                >  2:5249109/1‑93 (MQ=255)

                                                                           ctcATTACGGTATCCGCGGAGTTCGCTTGCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgc              <  2:3350676/91‑1 (MQ=255)

                                                                            ccATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcg             >  1:4604364/1‑93 (MQ=255)

                                                                             cATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcgc            <  2:1973771/93‑1 (MQ=255)

                                                                             cATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcgc            <  2:2270204/93‑1 (MQ=255)

                                                                              aTTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCACTGCCGCCTGCAAAGATTGCAGcgcg           >  1:4461830/1‑93 (MQ=255)

                                                                               ttACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCgcgg          >  2:5430097/1‑93 (MQ=255)

                                                                                  cGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGt       <  2:2901626/93‑1 (MQ=255)

                                                                                   ggTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTa      >  2:2147706/1‑93 (MQ=255)

                                                                                    gTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAg     <  2:3723965/93‑1 (MQ=255)

                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  <  2:6564207/93‑1 (MQ=255)

                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  <  2:6705351/93‑1 (MQ=255)

                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  >  1:4334023/1‑93 (MQ=255)

GTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG  >  NC_000913/3596205‑3596384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`