BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,614,182	T→C	F124L (TTC→CTC)	nikC →	NikC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,614,182	0	T	C	99.7%	1193.1 / ‑5.4	377	F124L (TTC→CTC)	nikC	NikC
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (194/181); total (195/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

Only 100 of 395 total aligned reads displayed.

GGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTG  >  NC_000913/3614092‑3614271

ggcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTc                                                                                         <  1:2461599/93‑1 (MQ=255)

 gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGctct                                                                                        >  1:3548634/1‑93 (MQ=255)

 gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGctct                                                                                        >  2:1589352/1‑93 (MQ=255)

 gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGctct                                                                                        <  2:1388088/93‑1 (MQ=255)

 gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGctct                                                                                        >  1:1787869/1‑93 (MQ=255)

 gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGctct                                                                                        >  1:5024406/1‑93 (MQ=255)

    gcgcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCtttt                                                                                     >  1:2085403/1‑92 (MQ=255)

    gcgcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTa                                                                                     <  1:3891363/93‑1 (MQ=255)

        cGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGt                                                                                 <  2:3548114/93‑1 (MQ=255)

        cGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGt                                                                                 <  1:7569333/93‑1 (MQ=255)

         gggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGtt                                                                                >  2:4466300/1‑93 (MQ=255)

          ggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTg                                                                               >  2:802311/1‑93 (MQ=255)

          ggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTg                                                                               >  1:4147777/1‑93 (MQ=255)

           gTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTgg                                                                              >  2:5190283/1‑93 (MQ=255)

            ttGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGc                                                                             <  1:6546142/93‑1 (MQ=255)

              gATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGt                                                                           >  1:1003495/1‑93 (MQ=255)

              gATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGGTGGCGt                                                                           >  1:6510457/1‑93 (MQ=255)

                 tGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCt                                                                        <  1:1264038/93‑1 (MQ=255)

                 tGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCt                                                                        <  2:2574863/93‑1 (MQ=255)

                     gggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGc                                                                    >  1:67199/1‑93 (MQ=255)

                     gggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGc                                                                    <  1:6896958/93‑1 (MQ=255)

                         gcgTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCg                                                                <  1:4762467/93‑1 (MQ=255)

                          cgTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCgg                                                               <  1:5066640/93‑1 (MQ=255)

                           gTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCggg                                                              >  2:466845/1‑93 (MQ=255)

                            ttGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGc                                                             >  1:4575050/1‑93 (MQ=255)

                            ttGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGc                                                             <  2:3309460/93‑1 (MQ=255)

                            ttGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGc                                                             <  1:1928454/93‑1 (MQ=255)

                            ttGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGc                                                             <  2:3498409/93‑1 (MQ=255)

                            ttGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGc                                                             >  2:169375/1‑93 (MQ=255)

                             tGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCt                                                            <  2:3373967/93‑1 (MQ=255)

                             tGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCt                                                            <  2:3959810/93‑1 (MQ=255)

                             tGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCt                                                            <  2:5422335/93‑1 (MQ=255)

                              gATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTg                                                           >  1:3415212/1‑93 (MQ=255)

                               aTCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGa                                                          >  1:1694652/1‑93 (MQ=255)

                                  aGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCa                                                       >  2:6965805/1‑93 (MQ=255)

                                    gCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAAc                                                     >  1:1351433/1‑93 (MQ=255)

                                     ccaccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACg                                                    <  2:4076288/93‑1 (MQ=255)

                                      caccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGt                                                   >  1:1589213/1‑93 (MQ=255)

                                      caccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGt                                                   >  1:7189033/1‑93 (MQ=255)

                                       cccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTa                                                  <  1:220011/92‑1 (MQ=255)

                                         caTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAAt                                                <  1:6197450/93‑1 (MQ=255)

                                          aTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACCGAAtt                                               >  2:5981867/1‑93 (MQ=255)

                                           tGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGTGTGCTCGGCACCGGGCTGACCAACGTAATTa                                              >  2:4260260/1‑93 (MQ=255)

                                           tGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTa                                              >  2:6782213/1‑93 (MQ=255)

                                           tGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTa                                              <  2:1862959/93‑1 (MQ=255)

                                           tGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTa                                              >  1:4209539/1‑93 (MQ=255)

                                            gcgcGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTat                                             <  2:7208446/93‑1 (MQ=255)

                                             cgcgTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatc                                            >  1:5375318/1‑93 (MQ=255)

                                                 tCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcca                                        >  1:3094338/1‑93 (MQ=255)

                                                  cGCCTATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccat                                       <  2:6143389/93‑1 (MQ=255)

                                                  cGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccat                                       <  2:7628110/93‑1 (MQ=255)

                                                  cGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccat                                       <  1:5718147/93‑1 (MQ=255)

                                                   gCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatc                                      >  2:5893418/1‑93 (MQ=255)

                                                   gCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatc                                      >  2:5681168/1‑93 (MQ=255)

                                                   gCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatc                                      >  1:6396804/1‑93 (MQ=255)

                                                   gCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatc                                      >  1:2822644/1‑93 (MQ=255)

                                                    ccGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcg                                     <  2:1496893/93‑1 (MQ=255)

                                                     cGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgc                                    >  2:5361243/1‑93 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                   >  1:2929125/1‑93 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                   >  2:289905/1‑93 (MQ=255)

                                                       atatGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGccc                                  >  2:7727166/1‑93 (MQ=255)

                                                       atatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTTACCAACGTAATTATCGCCATCGccc                                  <  2:2756884/93‑1 (MQ=255)

                                                       atatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGccc                                  >  1:5591256/1‑93 (MQ=255)

                                                       atatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGccc                                  <  2:6865715/93‑1 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                 >  1:2569552/1‑93 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                 >  2:7308418/1‑93 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                 >  1:4786707/1‑93 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                 >  1:7921103/1‑93 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                 <  2:373322/93‑1 (MQ=255)

                                                          tGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGGCCAACGTAATTATCGCCATCGCCCTGt                               >  1:6251124/1‑93 (MQ=255)

                                                          tGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGt                               >  2:2237744/1‑93 (MQ=255)

                                                            tttATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCg                             >  1:365284/1‑93 (MQ=255)

                                                               aTGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCAc                          >  1:953893/1‑93 (MQ=255)

                                                                 gACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTg                        >  2:1094569/1‑93 (MQ=255)

                                                                 gACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTg                        >  2:2612548/1‑93 (MQ=255)

                                                                  gacTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTgg                       <  2:6534952/91‑1 (MQ=255)

                                                                   ccTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTggg                      >  2:2812144/1‑93 (MQ=255)

                                                                   ccTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTggg                      <  2:6323161/93‑1 (MQ=255)

                                                                    cTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGc                     <  2:4791278/93‑1 (MQ=255)

                                                                    cTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGc                     >  1:6107055/1‑93 (MQ=255)

                                                                     ttCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCg                    >  2:2991605/1‑93 (MQ=255)

                                                                     ttCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCg                    <  2:3111738/93‑1 (MQ=255)

                                                                       cccGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTg                  <  1:7422772/93‑1 (MQ=255)

                                                                       cccGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTg                  <  2:7423180/93‑1 (MQ=255)

                                                                        ccGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTgg                 >  2:1969430/1‑93 (MQ=255)

                                                                         cGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGt                >  1:5813809/1‑93 (MQ=255)

                                                                           aCCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGGACCGGGATGACCAACGTAATTATCGCCATCGACCTGTCGCACTGGGCGTgggag              >  2:4596129/1‑90 (MQ=255)

                                                                           aCCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTAt              <  2:1787869/93‑1 (MQ=255)

                                                                           aCCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTAt              >  1:777543/1‑93 (MQ=255)

                                                                           aCCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTAt              >  1:7341809/1‑93 (MQ=255)

                                                                             cTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGc            <  1:889280/93‑1 (MQ=255)

                                                                              tCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCa           <  1:1523781/93‑1 (MQ=255)

                                                                               cGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCAc          <  1:707659/93‑1 (MQ=255)

                                                                               cGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCAc          <  1:4866720/93‑1 (MQ=255)

                                                                                gATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACg         >  2:2432821/1‑93 (MQ=255)

                                                                                gATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACg         >  1:3235959/1‑93 (MQ=255)

                                                                                 aTTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGc        >  2:5531954/1‑93 (MQ=255)

                                                                                 aTTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGc        >  2:494922/1‑93 (MQ=255)

                                                                                      gTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGt   <  1:1063048/93‑1 (MQ=255)

                                                                                       tCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTg  >  2:75513/1‑93 (MQ=255)

GGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTG  >  NC_000913/3614092‑3614271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`