BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	409,218	A→G	V9A (GTT→GCT)	yaiD ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	409,218	0	A	G	99.6%	805.5 / NA	254	V9A (GTT→GCT)	yaiD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (103/150); minor base T (0/1); total (103/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

TCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGC  >  NC_000913/409128‑409307

   tGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCAtt                                                                                      <  2:4508241/93‑1 (MQ=255)

    ggCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTa                                                                                     >  1:6350052/1‑93 (MQ=255)

      cTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTaaa                                                                                   <  2:5308709/93‑1 (MQ=255)

      cTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTaaa                                                                                   <  2:5604181/93‑1 (MQ=255)

       ttccGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAAt                                                                                  <  2:2717468/91‑1 (MQ=255)

       tGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAAt                                                                                  >  2:1708392/1‑93 (MQ=255)

       tGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAAt                                                                                  <  1:1403665/93‑1 (MQ=255)

       tGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAAt                                                                                  <  2:1111860/93‑1 (MQ=255)

        gCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAAtt                                                                                 >  1:5685229/1‑93 (MQ=255)

        gCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAAtt                                                                                 <  1:815699/93‑1 (MQ=255)

           gCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAAttttt                                                                              <  2:1319839/93‑1 (MQ=255)

             aTGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGa                                                                            >  1:5725560/1‑93 (MQ=255)

             aTGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGa                                                                            >  2:4914641/1‑93 (MQ=255)

              tGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGaa                                                                           >  2:6950757/1‑93 (MQ=255)

              tGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGaa                                                                           <  1:3924129/93‑1 (MQ=255)

               ggggTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAAc                                                                          >  1:6214165/1‑93 (MQ=255)

               ggggTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAAc                                                                          <  1:4061304/93‑1 (MQ=255)

               ggggTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAAc                                                                          <  1:700189/93‑1 (MQ=255)

                 ggTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACca                                                                        <  2:6227153/93‑1 (MQ=255)

                 ggTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACca                                                                        <  1:3851552/93‑1 (MQ=255)

                 ggTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACca                                                                        <  1:4571117/93‑1 (MQ=255)

                  gTAAATGGCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACcac                                                                       <  1:4131263/93‑1 (MQ=255)

                  gTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACcac                                                                       >  2:2217502/1‑93 (MQ=255)

                   tAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACcaca                                                                      <  2:4747125/93‑1 (MQ=255)

                   tAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACcaca                                                                      >  2:5722257/1‑93 (MQ=255)

                   tAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACcaca                                                                      >  1:225075/1‑93 (MQ=255)

                   tAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGGAAGCCATTAAATTTTTGAACcaca                                                                      >  2:5835434/1‑93 (MQ=255)

                     aaTGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGc                                                                    >  1:4697316/1‑93 (MQ=255)

                      aTGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCa                                                                   <  1:3409333/93‑1 (MQ=255)

                       tGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCAt                                                                  >  2:4792659/1‑93 (MQ=255)

                       tGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCAt                                                                  >  2:392551/1‑93 (MQ=255)

                        gCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATg                                                                 >  2:3247324/1‑93 (MQ=255)

                           aTCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATa                                                              >  1:1462736/1‑93 (MQ=255)

                           aTCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATa                                                              >  2:2553752/1‑93 (MQ=255)

                            tCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACTACAGCATGATaa                                                             <  2:3812896/93‑1 (MQ=255)

                            tCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATaa                                                             <  2:5539070/93‑1 (MQ=255)

                             cGAGGCTAGCTGTGTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAAt                                                            <  1:1001723/93‑1 (MQ=255)

                              gAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAAtt                                                           >  2:158670/1‑93 (MQ=255)

                                ggCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTc                                                         <  2:7680199/93‑1 (MQ=255)

                                ggCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTc                                                         >  1:1932350/1‑93 (MQ=255)

                                  cTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCa                                                       <  1:4174628/93‑1 (MQ=255)

                                  cTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCa                                                       <  2:5919810/93‑1 (MQ=255)

                                   tAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTGCAc                                                      >  1:4961118/1‑93 (MQ=255)

                                   tAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCAc                                                      >  1:3580516/1‑93 (MQ=255)

                                    tgCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACg                                                     <  2:6517276/92‑1 (MQ=255)

                                    aGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACg                                                     <  2:2797805/93‑1 (MQ=255)

                                        gTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCt                                                 <  1:2697143/93‑1 (MQ=255)

                                         tttttCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCtt                                                <  1:1132827/93‑1 (MQ=255)

                                          ttttCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTg                                               <  2:7376975/93‑1 (MQ=255)

                                            ttCCATCTCTCCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTc                                             <  1:3649912/93‑1 (MQ=255)

                                             tCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCg                                            >  2:6923690/1‑93 (MQ=255)

                                                 tctcTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTaa                                        <  1:5495292/93‑1 (MQ=255)

                                                 tctcTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTaa                                        <  1:3291551/93‑1 (MQ=255)

                                                     ttctGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAAttt                                    >  2:6988829/1‑93 (MQ=255)

                                                       ctGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAg                                  <  2:800912/93‑1 (MQ=255)

                                                       ctGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAg                                  >  1:3065044/1‑93 (MQ=255)

                                                         gCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCg                                <  1:1637709/93‑1 (MQ=255)

                                                         gCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCg                                >  2:3727637/1‑93 (MQ=255)

                                                         gCACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCg                                <  1:3603919/93‑1 (MQ=255)

                                                          cACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCgg                               <  1:7119090/93‑1 (MQ=255)

                                                          cACGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCgg                               <  1:6783526/93‑1 (MQ=255)

                                                           aCGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCggg                              <  1:7699247/93‑1 (MQ=255)

                                                           aCGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCggg                              >  1:3201215/1‑93 (MQ=255)

                                                           aCGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATGTAGCgga                              >  2:778043/1‑92 (MQ=255)

                                                            cGCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGc                             <  1:5338370/93‑1 (MQ=255)

                                                             gCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCa                            <  1:5020233/93‑1 (MQ=255)

                                                             gCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCa                            >  2:3712351/1‑93 (MQ=255)

                                                             gCAGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCa                            <  2:3883331/93‑1 (MQ=255)

                                                               aGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATg                          >  1:7303059/1‑93 (MQ=255)

                                                               aGCGAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATg                          >  1:7504674/1‑93 (MQ=255)

                                                                  gAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATa                       <  2:4378702/93‑1 (MQ=255)

                                                                  gAAATCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATa                       >  2:679118/1‑93 (MQ=255)

                                                                    aaTCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAAc                     <  2:5994310/93‑1 (MQ=255)

                                                                    aaTCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAAc                     >  2:6887448/1‑93 (MQ=255)

                                                                    aaTCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAAc                     <  1:7807554/93‑1 (MQ=255)

                                                                    aaTCTCGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAAc                     >  1:2872924/1‑93 (MQ=255)

                                                                      tctcGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGa                   >  1:7059311/1‑93 (MQ=255)

                                                                       ctcGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGaa                  <  2:933179/93‑1 (MQ=255)

                                                                        tcGCGGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAAt                 >  1:4359737/1‑93 (MQ=255)

                                                                         cgcgGCTAAGACGGTAATCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAAtt                <  1:953199/93‑1 (MQ=255)

                                                                         cgcgGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAAtt                <  1:7376160/93‑1 (MQ=255)

                                                                          gcgGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTg               >  2:6362239/1‑93 (MQ=255)

                                                                           cgGCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGt              <  1:5161178/93‑1 (MQ=255)

                                                                            ggCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTc             <  2:360461/93‑1 (MQ=255)

                                                                             gCTAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcg            <  1:6923691/93‑1 (MQ=255)

                                                                               tAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcggc          >  1:7758494/1‑93 (MQ=255)

                                                                               tAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcggc          >  1:1503865/1‑93 (MQ=255)

                                                                               tAAGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcggc          >  2:4130988/1‑93 (MQ=255)

                                                                                aaGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCTCGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcggcg         <  1:4117058/93‑1 (MQ=255)

                                                                                aaGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcggcg         <  1:603548/93‑1 (MQ=255)

                                                                                aaGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcggcg         <  2:7138394/93‑1 (MQ=255)

                                                                                 aGACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcggcgg        >  1:5394095/1‑93 (MQ=255)

                                                                                  gACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCggcggc       >  2:14564/1‑93 (MQ=255)

                                                                                  gACGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCggcggc       >  2:2991331/1‑93 (MQ=255)

                                                                                    cGGTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCt     <  2:5758210/93‑1 (MQ=255)

                                                                                     ggTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGTGGGCATGATAACGAATTGTCGGCGGCCtt    <  1:7843407/93‑1 (MQ=255)

                                                                                     ggTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCtt    <  1:5894213/93‑1 (MQ=255)

                                                                                      gTAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTg   <  2:7184706/93‑1 (MQ=255)

                                                                                       tAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGc  >  1:5846231/1‑93 (MQ=255)

                                                                                       tAAGCCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGc  >  1:2129774/1‑93 (MQ=255)

TCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGC  >  NC_000913/409128‑409307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`