BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,649,511	G→A	E197E (GAG→GAA)	yhiS →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,649,511	0	G	A	99.8%	1499.0 / ‑6.3	413	E197E (GAG→GAA)	yhiS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (230/182); total (231/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 420 total aligned reads displayed.

TAATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGT  >  NC_000913/3649421‑3649600

tAATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAAca                                                                                         >  1:966712/1‑93 (MQ=255)

  aTTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAAcaca                                                                                       <  2:1233447/93‑1 (MQ=255)

   ttttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAg                                                                                      >  2:3896207/1‑93 (MQ=255)

   ttttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTATATCAAAAAATGACGAAGTTATATATTGAACACAg                                                                                      <  2:5354934/93‑1 (MQ=255)

    tttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGt                                                                                     <  1:6535040/93‑1 (MQ=255)

    tttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGt                                                                                     <  2:3554880/93‑1 (MQ=255)

      tATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGa                                                                                   >  2:4178474/1‑93 (MQ=255)

      tATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGa                                                                                   <  1:2383896/93‑1 (MQ=255)

      tATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGa                                                                                   >  1:2627033/1‑93 (MQ=255)

       aTTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaa                                                                                  >  1:4300012/1‑93 (MQ=255)

        ttGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaaa                                                                                 <  2:6605414/93‑1 (MQ=255)

          gCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAAt                                                                               <  2:4013023/93‑1 (MQ=255)

            tGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaa                                                                             <  1:3102499/93‑1 (MQ=255)

             gAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaaa                                                                            >  2:4939706/1‑93 (MQ=255)

             gAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaaa                                                                            <  2:7115657/93‑1 (MQ=255)

              aacaacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaaaa                                                                           <  1:5581024/93‑1 (MQ=255)

               acaacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaaaaa                                                                          <  1:3399938/93‑1 (MQ=255)

                 aacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACa                                                                        >  1:2933447/1‑93 (MQ=255)

                 aacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACa                                                                        >  1:1939458/1‑93 (MQ=255)

                  acGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAg                                                                       <  1:1735685/93‑1 (MQ=255)

                  acGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAg                                                                       >  2:1843183/1‑93 (MQ=255)

                     aGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGaa                                                                    >  1:2116479/1‑92 (MQ=255)

                     aGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGAt                                                                    <  1:4840494/93‑1 (MQ=255)

                     aGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGAt                                                                    >  2:6307440/1‑93 (MQ=255)

                      gCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATg                                                                   >  1:5819550/1‑93 (MQ=255)

                        tGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGaa                                                                 <  1:7448529/93‑1 (MQ=255)

                        tGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGaa                                                                 <  2:3707004/93‑1 (MQ=255)

                         gACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGaaa                                                                >  2:2603428/1‑93 (MQ=255)

                          aCTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAg                                                               >  1:5008737/1‑93 (MQ=255)

                            ttACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTc                                                             >  2:2273581/1‑93 (MQ=255)

                            ttACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTc                                                             >  1:5202195/1‑93 (MQ=255)

                            ttACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTc                                                             <  1:551039/93‑1 (MQ=255)

                              aCCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGACAACAGTGAAACTAAAAACAGGATGAAAGTCtt                                                           >  2:567621/1‑93 (MQ=255)

                              aCCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCtt                                                           >  2:59811/1‑93 (MQ=255)

                               ccAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCttt                                                          >  2:6075459/1‑93 (MQ=255)

                                cAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAACAACAGGCTGAAAGTCTTTg                                                         >  1:5791910/1‑93 (MQ=255)

                                cAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTg                                                         >  2:4142897/1‑93 (MQ=255)

                                  aCTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGcc                                                       >  2:3793109/1‑93 (MQ=255)

                                  aCTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGcc                                                       <  1:3829762/93‑1 (MQ=255)

                                   cTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCg                                                      >  1:1122989/1‑93 (MQ=255)

                                    tAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCgg                                                     >  1:6675430/1‑93 (MQ=255)

                                    tAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCgg                                                     >  2:1413922/1‑93 (MQ=255)

                                      atatAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGaa                                                   >  2:3347186/1‑93 (MQ=255)

                                       tataAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAAc                                                  >  2:3249279/1‑93 (MQ=255)

                                       tataAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAAc                                                  >  1:6207561/1‑93 (MQ=255)

                                       tataAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAAc                                                  <  1:4614829/93‑1 (MQ=255)

                                       tataAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAAc                                                  >  2:2131698/1‑93 (MQ=255)

                                         taAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCa                                                >  2:2678502/1‑93 (MQ=255)

                                          aaaaGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCaa                                               >  2:7383213/1‑93 (MQ=255)

                                           aaaGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAAc                                              >  1:5288158/1‑93 (MQ=255)

                                            aaGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACt                                             <  2:3623301/93‑1 (MQ=255)

                                             aGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACtt                                            >  2:606107/1‑93 (MQ=255)

                                               atatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTa                                          <  1:6991785/93‑1 (MQ=255)

                                                tatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAt                                         >  1:4481273/1‑93 (MQ=255)

                                                 atTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAtt                                        >  2:7872654/1‑93 (MQ=255)

                                                 atTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAtt                                        >  1:2297336/1‑93 (MQ=255)

                                                  ttCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTg                                       <  2:7683386/93‑1 (MQ=255)

                                                  ttCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTg                                       >  1:7599798/1‑93 (MQ=255)

                                                  ttCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTAAAAATAAAAACAGGAAGAAAGTCTTTGCCGCAACCAACTTTAttt                                       >  2:5248340/1‑92 (MQ=255)

                                                   tCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGa                                      <  2:7535335/93‑1 (MQ=255)

                                                    cAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAt                                     >  2:4585667/1‑93 (MQ=255)

                                                       aGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTc                                  >  2:4417550/1‑93 (MQ=255)

                                                       aGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTc                                  <  1:3988948/93‑1 (MQ=255)

                                                         ccTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCaa                                >  1:1578036/1‑93 (MQ=255)

                                                         ccTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCaa                                >  2:6538705/1‑93 (MQ=255)

                                                         ccTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCaa                                >  1:7420536/1‑93 (MQ=255)

                                                         ccTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCaa                                >  2:3824470/1‑93 (MQ=255)

                                                           tGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAata                              >  1:7846638/1‑93 (MQ=255)

                                                           tGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGACCCAACTTTATTGATTTCAata                              <  1:4196687/93‑1 (MQ=255)

                                                            gTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAatat                             >  2:6670254/1‑93 (MQ=255)

                                                            gTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAatat                             >  2:7276885/1‑93 (MQ=255)

                                                             tATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATg                            >  1:2879741/1‑93 (MQ=255)

                                                              aTCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATga                           <  1:7072607/93‑1 (MQ=255)

                                                               tCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgac                          <  1:1291329/93‑1 (MQ=255)

                                                               tCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgac                          >  2:3022049/1‑93 (MQ=255)

                                                               tCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgac                          >  1:7127748/1‑93 (MQ=255)

                                                                 aaaaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacag                        <  1:2731497/93‑1 (MQ=255)

                                                                 aaaaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacag                        <  2:6847397/93‑1 (MQ=255)

                                                                  aaaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacagg                       >  1:5924871/1‑93 (MQ=255)

                                                                   aaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacagga                      >  1:5088211/1‑93 (MQ=255)

                                                                    aaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacaggac                     >  1:1173310/1‑93 (MQ=255)

                                                                    aaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacaggac                     >  2:2749763/1‑93 (MQ=255)

                                                                       tGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGa                  >  1:3681267/1‑93 (MQ=255)

                                                                       tGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGa                  <  1:3251154/93‑1 (MQ=255)

                                                                        gACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGaa                 >  2:2034860/1‑93 (MQ=255)

                                                                          cGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAAcc               <  1:329686/93‑1 (MQ=255)

                                                                           gAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCt              <  2:476087/93‑1 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTcc          >  1:3554544/1‑93 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTcc          <  1:6335394/93‑1 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTcc          <  2:4499571/93‑1 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGAcc          >  2:5131437/1‑93 (MQ=255)

                                                                                   atatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgt      <  1:1340290/93‑1 (MQ=255)

                                                                                    tatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgtt     >  2:4662019/1‑93 (MQ=255)

                                                                                    tatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgtt     >  2:5833775/1‑93 (MQ=255)

                                                                                    tatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgtt     >  2:244728/1‑93 (MQ=255)

                                                                                     atTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttc    >  1:544198/1‑93 (MQ=255)

                                                                                     atTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttc    <  2:1573998/93‑1 (MQ=255)

                                                                                       tGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttcgt  <  2:5502843/93‑1 (MQ=255)

                                                                                       tGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttcgt  >  2:1748075/1‑93 (MQ=255)

                                                                                       tGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGggtcgt  >  1:299873/1‑93 (MQ=255)

TAATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGT  >  NC_000913/3649421‑3649600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`