Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,670,614 C→T R215C (CGT→TGT)  yhjC → putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,670,6140CT99.8% 1425.2 / ‑5.8 398R215C (CGT→TGT) yhjCputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (197/200);  total (197/201)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.03e-01

Only 100 of 410 total aligned reads displayed.

CTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTG  >  NC_000913/3670524‑3670703
                                                                                          |                                                                                         
cTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAgtgtgt                                                                                         <  2:4149834/93‑1 (MQ=255)
 tGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGtt                                                                                        <  1:7525936/93‑1 (MQ=255)
 tGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGtt                                                                                        >  1:6187203/1‑93 (MQ=255)
  gACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTta                                                                                       >  2:7932155/1‑93 (MQ=255)
  gACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTta                                                                                       <  2:1638514/93‑1 (MQ=255)
  gACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTta                                                                                       <  1:629787/93‑1 (MQ=255)
  gACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTta                                                                                       <  2:5551601/93‑1 (MQ=255)
   aCCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTtat                                                                                      <  2:20065/93‑1 (MQ=255)
    ccATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTtata                                                                                     <  2:1918241/93‑1 (MQ=255)
    ccATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTtata                                                                                     <  1:5043794/93‑1 (MQ=255)
     cATGGTCAAATGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATac                                                                                    <  1:476040/93‑1 (MQ=255)
       tGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCACTAGTGTGTTATagac                                                                                  >  1:2087769/1‑93 (MQ=255)
        ggTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACAcc                                                                                 <  1:4291038/93‑1 (MQ=255)
        ggTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACAcc                                                                                 >  2:2394802/1‑93 (MQ=255)
         gTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCg                                                                                <  1:6689000/93‑1 (MQ=255)
          taaaCTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGc                                                                               <  2:762410/91‑1 (MQ=255)
          tCAACTGTGCCAGTCCGCACTATCTGACGCCCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGc                                                                               <  1:7086844/93‑1 (MQ=255)
             aCTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCAcc                                                                            >  2:5466837/1‑93 (MQ=255)
             aCTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCAcc                                                                            <  2:7342004/93‑1 (MQ=255)
              cTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCt                                                                           <  2:1748879/93‑1 (MQ=255)
               tgtgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCACAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTg                                                                          <  2:7011562/93‑1 (MQ=255)
               tgtgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTg                                                                          <  2:3685957/93‑1 (MQ=255)
               tgtgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTg                                                                          <  1:29822/93‑1 (MQ=255)
               tgtgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTg                                                                          >  1:6867569/1‑93 (MQ=255)
               tgtgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTg                                                                          >  1:1804112/1‑93 (MQ=255)
                gtgCCAGTCCGCTCTATCTGACCCGCTTTGGTTATCCGCAAATCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTgg                                                                         <  1:5981182/93‑1 (MQ=255)
                gtgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTgg                                                                         <  2:2559932/93‑1 (MQ=255)
                gtgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTgg                                                                         <  1:1620236/93‑1 (MQ=255)
                   ccAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAATCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGgtg                                                                      <  2:6615644/93‑1 (MQ=255)
                     aGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTa                                                                    <  1:3214471/93‑1 (MQ=255)
                       tCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACa                                                                  >  2:1365908/1‑93 (MQ=255)
                       tCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACa                                                                  >  2:6526783/1‑93 (MQ=255)
                       tCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACa                                                                  >  2:4059569/1‑93 (MQ=255)
                         cGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATc                                                                >  2:6155947/1‑93 (MQ=255)
                            aCTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGt                                                             >  2:3898672/1‑93 (MQ=255)
                            aCTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGt                                                             >  1:790253/1‑93 (MQ=255)
                             cTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGtt                                                            >  1:5860408/1‑93 (MQ=255)
                             cTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTAAATCCGtt                                                            >  1:5337509/1‑93 (MQ=255)
                              tATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTa                                                           <  1:2425790/93‑1 (MQ=255)
                              tATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTa                                                           <  1:4568254/93‑1 (MQ=255)
                              tATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTa                                                           <  2:4466147/93‑1 (MQ=255)
                               aTCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAg                                                          >  2:2700055/1‑93 (MQ=255)
                               aTCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAg                                                          >  1:4826700/1‑93 (MQ=255)
                                tCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAgg                                                         >  1:4720753/1‑93 (MQ=255)
                                  tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGtt                                                       <  2:3354560/93‑1 (MQ=255)
                                  tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGtt                                                       <  1:1995739/93‑1 (MQ=255)
                                   gACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGttt                                                      <  2:7215680/93‑1 (MQ=255)
                                   gACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGttt                                                      <  2:591038/93‑1 (MQ=255)
                                         cTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGt                                                <  2:5809547/93‑1 (MQ=255)
                                         cTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGt                                                <  1:3415545/93‑1 (MQ=255)
                                          tttGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGtt                                               <  2:111962/93‑1 (MQ=255)
                                           ttGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTg                                              <  1:5595753/93‑1 (MQ=255)
                                            tGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCGCCTGGGTGTACATCCGTTAGGTTTTGAGGTTGc                                             >  2:6479198/1‑93 (MQ=255)
                                            tGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGc                                             >  1:3816466/1‑93 (MQ=255)
                                             ggTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                            >  1:226642/1‑93 (MQ=255)
                                              gTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCa                                           <  1:6936858/93‑1 (MQ=255)
                                              gTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCa                                           <  1:7747583/93‑1 (MQ=255)
                                               ttATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAg                                          <  2:3453084/93‑1 (MQ=255)
                                               ttATCCGCAAAGCACCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAg                                          <  2:7796998/93‑1 (MQ=255)
                                                tATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGc                                         <  2:5688773/93‑1 (MQ=255)
                                                  tCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGt                                       >  2:156051/1‑93 (MQ=255)
                                                  tCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGt                                       >  2:2985413/1‑93 (MQ=255)
                                                    cGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTa                                     <  2:7712305/93‑1 (MQ=255)
                                                    cGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTa                                     >  1:4009657/1‑93 (MQ=255)
                                                     gCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTaa                                    >  2:3397725/1‑93 (MQ=255)
                                                     gCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTaa                                    <  1:850569/93‑1 (MQ=255)
                                                      cAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAAt                                   <  1:2684955/93‑1 (MQ=255)
                                                       aaaGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATg                                  >  2:2202329/1‑93 (MQ=255)
                                                        aaGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACATCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATgg                                 >  1:2558657/1‑93 (MQ=255)
                                                         aGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGc                                >  2:2076573/1‑93 (MQ=255)
                                                         aGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGc                                <  2:6317066/93‑1 (MQ=255)
                                                          gCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCg                               >  1:1726131/1‑93 (MQ=255)
                                                            cccGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTc                             >  1:6424448/1‑93 (MQ=255)
                                                             ccGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTcc                            >  2:3145846/1‑93 (MQ=255)
                                                              cGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCa                           <  2:2893395/93‑1 (MQ=255)
                                                               gatgatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAg                          <  1:7209316/93‑1 (MQ=255)
                                                               gatgatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAg                          >  1:2797796/1‑93 (MQ=255)
                                                               gatgatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAg                          >  1:395222/1‑93 (MQ=255)
                                                                  gatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTgg                       >  1:291221/1‑93 (MQ=255)
                                                                   atCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGt                      >  2:7466805/1‑93 (MQ=255)
                                                                   atCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGt                      >  1:6627921/1‑93 (MQ=255)
                                                                    tCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGCGGTTGACAGCGTTAATGGCGTCCAGTCGtt                     >  1:3485597/1‑93 (MQ=255)
                                                                        aCTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAg                 <  1:100598/93‑1 (MQ=255)
                                                                        aCTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAg                 <  2:6601261/93‑1 (MQ=255)
                                                                        aCTTCACACCCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGCTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAg                 >  2:5031455/1‑93 (MQ=255)
                                                                          ttCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTc               <  1:4953155/93‑1 (MQ=255)
                                                                          ttCACACGCAACAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTc               <  1:1404398/93‑1 (MQ=255)
                                                                              cacGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggc           <  2:6804811/93‑1 (MQ=255)
                                                                                  cAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCa       >  1:1238951/1‑93 (MQ=255)
                                                                                   aaTAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTTCAGTGGTTTAAGTCTGGCGGCAt      >  2:4303420/1‑93 (MQ=255)
                                                                                   aaTAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCAt      <  1:7615387/93‑1 (MQ=255)
                                                                                   aaTAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGCTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCAt      >  1:650168/1‑93 (MQ=255)
                                                                                    aTAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATg     >  1:6985757/1‑93 (MQ=255)
                                                                                     tAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGt    >  2:6728623/1‑93 (MQ=255)
                                                                                     tAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGt    >  1:3268444/1‑93 (MQ=255)
                                                                                     tAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGt    >  2:4736630/1‑93 (MQ=255)
                                                                                      aGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGtt   <  2:6883810/93‑1 (MQ=255)
                                                                                      aGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGtt   <  2:3541322/93‑1 (MQ=255)
                                                                                       gtgtgtTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTg  <  2:5128742/93‑1 (MQ=255)
                                                                                       gtgtgtTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTg  >  1:5397902/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTG  >  NC_000913/3670524‑3670703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑